LOCUS       sequence-271344-        9716 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentivirus for base editing activatable mScarletI expression in 
            mammalian cells. All-in-one vector with sgmScarletGO. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9716)
  AUTHORS   Katti A, Foronda M, Zimmerman J, Diaz B, Zafra MP, Goswami S, Dow LE
  TITLE     GO: a functional reporter system to identify and enrich base editing
            activity.
  JOURNAL   Nucleic Acids Res. 2020 Feb 29. pii: 5766653. doi: 
            10.1093/nar/gkaa124.
  PUBMED    32112097
REFERENCE   2  (bases 1 to 9716)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Feb 29. pii: 5766653. doi: 10.1093/nar/gkaa124."
COMMENT     Sequence Label: pRRL-mU6-mScarletGO-P2A-Blas-PGK-Neo
FEATURES             Location/Qualifiers
     source          1..9716
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1806..1923
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1982..2295
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for mouse U6 snRNA (Das 
                     et al., 1988)"
     primer_bind     2214..2234
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     primer_bind     3447..3470
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     CDS             3569..3625
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     promoter        4094..4593
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4129..4152)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4526..4545
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             4607..5410
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4670..4689)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5280..5299
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    5444..6032
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5497..5517)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5915..5926)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6239..6472
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6544..6665
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6581..6600)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6635..6654
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6705..6840
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6860..6879)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6861..6879)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6889..6906)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6889..6905)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6898..6920)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7047..7502
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7134..7153)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7344..7365
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7528..7632
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7633..7701,7702..8493)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7851..7870)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8664..9252
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9153..9172
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9406..9423
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9540..9561
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9576..9581,9582..9599,9600..9606)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9614..9630
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9619..9641
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9638..9654
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9638..9654
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9673..9693
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9675..9693
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
     1741 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcgg
     1801 ttaactttta aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac
     1861 ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat
     1921 tttatcgatc acgagactag cctcgatcgc aattctgcag acaaatggct ctagatctag
     1981 agatccgacg ccgccatctc taggcccgcg ccggccccct cgcacagact tgtgggagaa
     2041 gctcggctac tcccctgccc cggttaattt gcatataata tttcctagta actatagagg
     2101 cttaatgtgc gataaaagac agataatctg ttctttttaa tactagctac attttacatg
     2161 ataggcttgg atttctataa gagatacaaa tactaaatta ttattttaaa aaacagcaca
     2221 aaaggaaact caccctaact gtaaagtaat tgtgtgtttt gagactataa atatgcatgc
     2281 gagaaaagcc ttgtttgacg acggtgagca agggcggttt aagagctatg ctggaaacag
     2341 catagcaagt ttaaataagg ctagtccgtt atcaacttga aaaagtggca ccgagtcggt
     2401 gcttttttgt tttaagtctc gagatcgagg gctagcgact agctgcagta acgccatttt
     2461 gcaaggcatg gaaaaatacc aaaccaagaa tagagaagtt cagatcaagg gcgggtacat
     2521 gaaaatagct aacgttgggc caaacaggat atctgcggtg agcagtttcg gccccggccc
     2581 ggggccaaga acagatggtc accgcagttt cggccccggc ccgaggccaa gaacagatgg
     2641 tccccagata tggcccaacc ctcagcagtt tcttaagacc catcagatgt ttccaggctc
     2701 ccccaaggac ctgaaatgac cctgcgcctt atttgaatta accaatcagc ctgcttctcg
     2761 cttctgttcg cgcgcttctg cttcccgagc tctataaaag agctcacaac ccctcactcg
     2821 gcgcgccagt cctccgacag aattcgcgac gacggtgagc aagggcgagg ccgtgatcaa
     2881 ggagttcatg aggttcaagg tgcacatgga gggctccatg aacggtcacg agttcgaaat
     2941 cgagggcgaa ggcgagggca ggccctacga gggcacccag accgccaagc tgaaggtgac
     3001 aaagggcggc cccctgccct tctcctggga catcctgtcc cctcagttca tgtacggctc
     3061 cagggccttc atcaagcacc ccgccgacat ccccgactac tacaagcagt ccttccccga
     3121 gggcttcaag tgggagaggg tgatgaactt cgaggacggc ggcgccgtga ccgtgaccca
     3181 ggacacctcc ctggaggacg gcaccctgat ctacaaggtg aagctgaggg gcaccaactt
     3241 ccctcctgac ggccccgtga tgcagaagaa gacaatgggc tgggaggcct ccaccgagag
     3301 gctgtacccc gaggacggcg tgctgaaggg cgacatcaag atggccctga ggctgaagga
     3361 cggcggcaga tacctggccg acttcaagac cacctacaag gccaagaagc ccgtgcagat
     3421 gcccggcgcc tacaacgtgg acaggaagct ggacatcacc tcccacaacg aggactacac
     3481 cgtggtggag cagtacgaga ggtccgaggg caggcactcc accggcggca tggacgagct
     3541 gtataagaaa gcggccgcag gcagcggcgc caccaacttc agcctgctga agcaggccgg
     3601 cgacgtggag gagaaccccg gccccatgaa gaccttcaac atctctcagc aggaccttga
     3661 gctggtggaa gttgccactg agaaaatcac catgctctat gaggacaaca agcaccatgt
     3721 tggtgctgcc atcaggacca agacaggaga aatcatctct gctgtccaca ttgaagccta
     3781 cattggcagg gtcactgtgt gtgcagaggc cattgccatt gggtctgcag tctccaatgg
     3841 gcagaaggac tttgacacca ttgtggctgt gaggcacccc tacagtgatg aggtggacag
     3901 gtccatcaga gtggtgtccc cctgtggcat gtgcagggag ctcatctcag actatgcccc
     3961 tgattgcttt gttctgattg agatgaatgg caagctggtc aagacaacca ttgaggagct
     4021 gattccactg aaatacacca gaaactaatg tacaagtaat gacgtatgca tgcaattgac
     4081 gcgtaattct accgggtagg ggaggcgctt ttcccaaggc agtctggagc atgcgcttta
     4141 gcagccccgc tgggcacttg gcgctacaca agtggcctct ggcctcgcac acattccaca
     4201 tccaccggta ggcgccaacc ggctccgttc tttggtggcc ccttcgcgcc accttctact
     4261 cctcccctag tcaggaagtt cccccccgcc ccgcagctcg cgtcgtgcag gacgtgacaa
     4321 atggaagtag cacgtctcac tagtctcgtg cagatggaca gcaccgctga gcaatggaag
     4381 cgggtaggcc tttggggcag cggccaatag cagctttgct ccttcgcttt ctgggctcag
     4441 aggctgggaa ggggtgggtc cgggggcggg ctcaggggcg ggctcagggg cggggcgggc
     4501 gcccgaaggt cctccggagg cccggcattc tgcacgcttc aaaagcgcac gtctgccgcg
     4561 ctgttctcct cttcctcatc tccgggcctt tcgacctgca gccaatatgg gatcggccat
     4621 tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta
     4681 tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca
     4741 ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga
     4801 cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga
     4861 cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct
     4921 cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg
     4981 gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga
     5041 gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca
     5101 tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga
     5161 ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg
     5221 cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc
     5281 gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt
     5341 gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga
     5401 gttcttctga ggggatccgt cgacttcgaa acgcgtctgg aacaatcaac ctctggatta
     5461 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg
     5521 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc
     5581 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca
     5641 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac
     5701 cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact
     5761 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc
     5821 cgtggtgttg tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg
     5881 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc
     5941 ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac
     6001 gagtcggatc tccctttggg ccgcctcccc gcctggaatt aattctgcag tcgagaccta
     6061 gaaaaacatg gagcaatcac aagtagcaat acagcagcta ccaatgctga ttgtgcctgg
     6121 ctagaagcac aagaggagga ggaggtgggt tttccagtca cacctcaggt acctttaaga
     6181 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg
     6241 gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc
     6301 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     6361 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     6421 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt
     6481 tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag
     6541 aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     6601 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     6661 tcttatcatg tctggctcta gctatcccgc ccctaactcc gcccatcccg cccctaactc
     6721 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     6781 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     6841 tagggacgta cccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt
     6901 tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca
     6961 tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca
     7021 gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg
     7081 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     7141 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     7201 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     7261 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     7321 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     7381 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     7441 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat
     7501 ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     7561 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     7621 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     7681 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     7741 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     7801 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     7861 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     7921 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     7981 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     8041 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     8101 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     8161 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     8221 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     8281 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     8341 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     8401 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     8461 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     8521 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     8581 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     8641 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     8701 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     8761 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     8821 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     8881 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     8941 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     9001 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     9061 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     9121 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     9181 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     9241 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     9301 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     9361 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     9421 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
     9481 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
     9541 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt
     9601 atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat
     9661 tacgccaagc gcgcaattaa ccctcactaa agggaacaaa agctggagct gcaagc
//
