LOCUS       sequence-271350-       10904 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Heterologous, copper-inducible expression of (E)-alpha-bisabolene 
            synthase from Abies grandis (AgBIS) in Synechocystis sp. PCC 6803. 
            The AgBIS CDS is fused to a C-termial Flag-tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10904)
  AUTHORS   Dienst D, Wichmann J, Mantovani O, Rodrigues JS, Lindberg P
  TITLE     High density cultivation for efficient sesquiterpenoid biosynthesis 
            in Synechocystis sp. PCC 6803.
  JOURNAL   Sci Rep. 2020 Apr 3;10(1):5932. doi: 10.1038/s41598-020-62681-w.
  PUBMED    32246065
REFERENCE   2  (bases 1 to 10904)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2020-04-3"; volume: "10(1):5932. doi"; pages: " 
            10.1038/s41598-020-62681-w"
COMMENT     Sequence Label: pSHDY*in_PpetE:BCD2:AgBIS:Flag
FEATURES             Location/Qualifiers
     source          1..10904
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(160..975)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(160..975)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     883..902
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     promoter        complement(976..1067)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1093..1110)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(1154..1945)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVMHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_feature    2151..2172
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      2175..2246
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2262..2289
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     RBS             2803..2811
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             5287..5310
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     terminator      5331..5402
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      5418..5445
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(5658..6509)
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETASLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     CDS             complement(6496..7335)
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             complement(7846..8817)
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     oriT            10056..10143
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     rep_origin      10485..10879
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
ORIGIN
        1 ggtaccgagt cgatgtcgac taacgaccct gccctgaacc gacgacaagc tgacgaccgg
       61 gtctccgcaa gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
      121 taaatacatt caaatatgta tccgctcatg aattaattct tagaaaaact catcgagcat
      181 caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg
      241 tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta
      301 tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa
      361 aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa
      421 aagtttatgc atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa
      481 atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gacgaaatac
      541 gcggtcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac
      601 tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc
      661 tgttttcccg gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg
      721 cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt
      781 aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt
      841 cccatacaat cgatagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata
      901 cccatataaa tcagcatcca tgttggaatt taatcgcggc ctagagcaag acgtttcccg
      961 ttgaatatgg ctcatactct tcctttttca atattattga agcatttatc agggttattg
     1021 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gcatgcagcg
     1081 ctcttccgct tcctcgctca ctgactcgct acgctcggtc gttcgactct cgagagcggc
     1141 cgaagcttag acattatttg ccgactacct tggtgatctc gcctttcacg tagtggacaa
     1201 attcttccaa ctgatctgcg cgcgaggcca agcgatcttc ttcttgtcca agataagcct
     1261 gtctagcttc aagtatgacg ggctgatact gggccggcag gcgctccatt gcccagtcgg
     1321 cagcgacatc cttcggcgcg attttgccgg ttactgcgct gtaccaaatg cgggacaacg
     1381 taagcactac atttcgctca tcgccagccc agtcgggcgg cgagttccat agcgttaagg
     1441 tttcatttag cgcctcaaat agatcctgtt caggaaccgg atcaaagagt tcctccgccg
     1501 ctggacctac caaggcaacg ctatgttctc ttgcttttgt cagcaagata gccagatcaa
     1561 tgtcgatcgt ggctggctcg aagatacctg caagaatgtc attgcgctgc cattctccaa
     1621 attgcagttc gcgcttagct ggataacgcc acggaatgat gtcgtcgtgc ataacaatgg
     1681 tgacttctac agcgcggaga atctcgctct ctccagggga agccgaagtt tccaaaaggt
     1741 cgttgatcaa agctcgccgc gttgtttcat caagccttac ggtcaccgta accagcaaat
     1801 caatatcact gtgtggcttc aggccgccat ccactgcgga gccgtacaaa tgtacggcca
     1861 gcaacgtcgg ttcgagatgg cgctcgatga cgccaactac ctctgatagt tgagtcgata
     1921 cttcggcgat caccgcttcc ctcatgatgt ttaactttgt tttagggcga ctgccctgct
     1981 gcgtaacatc gttgctgctc cataacatca aacatcgacc cacggcgtaa cgcgcttgct
     2041 gcttggatgc ccgaggcata gactgtaccc caaaaaaaca gtcataacaa gccatgaaaa
     2101 ccgccactgc gccgttacca ccgctgcgtt cggtcaaggt tctggaccag gaattcgcgg
     2161 ccgcttctag agatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta
     2221 tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc
     2281 ctttctgcgt ttttacccta cacttaaacc ccatctcaat acttcccatc aaatccaacc
     2341 taactcatac attccaaact aatctaactt cttccatcat taaccacacc tctctatcat
     2401 cacctattcc cctctatctc cctcaaattc tttcacatat taaaaactta ctcttcctcc
     2461 tttcctcctt aaaatactaa ataactctta aaatagaagg gatagcaagc taatttttat
     2521 gacggcgatc gccaaaaaca aagaaaattc agcaattacc gtgggtagca aaaaatcccc
     2581 atctaaagtt cagtaaatat agctagaaca accaagcatt ttcggcaaag tactattcag
     2641 atagaacgag aaatgagctt gttctatccg cccggggctg aggctgtata atctacgacg
     2701 ggctgtcaaa cattgtgata ccatgggcag aagggcccaa gttcacttaa aaaggagatc
     2761 aacaatgaaa gcaattttcg tactgaaaca tcttaatcat gctaaggagg ttttctaatg
     2821 gctagtgtga gtgccgttag caaagtgagt tctctggtgt gcgatctgtc tagcacttcc
     2881 gggctcattc ggcgtacagc caaccctcat ccaaatgtgt gggggtacga tctggtgcac
     2941 tctttgaaat ctccctatat cgattcttcc tatcgtgaac gggctgaagt gctcgtttct
     3001 gagatcaaag ccatgctgaa tcccgctatt accggcgacg gggaatccat gattacccca
     3061 tccgcctatg atactgcctg ggttgcccgc gttcccgcaa ttgatggtag cgcccgtccc
     3121 caatttcccc aaacggttga ctggattctg aagaaccagt taaaagacgg ctcttgggga
     3181 atccaatccc actttctatt gtccgatcgg ttattagcca ccttgagctg cgtcttagta
     3241 ttattgaagt ggaacgttgg cgatctccaa gtggaacagg gcattgaatt tattaaaagt
     3301 aatctcgagc tagtcaagga cgaaacggat caggatagtt tggtgacaga ttttgagatt
     3361 attttcccca gtttactgcg tgaagcgcag tccctgcggt tgggactgcc gtatgactta
     3421 ccttatatcc atttgttaca aacaaaacga caagaacgat tagctaagct gtctcgcgag
     3481 gaaatctacg ctgtgcctag tcctctgtta tactccttag aaggtattca ggatatcgtc
     3541 gaatgggaac gtattatgga agttcagagt caagacggaa gttttttgag cagcccagcc
     3601 tccactgctt gtgtgtttat gcataccggt gatgccaaat gtttagaatt tctcaattct
     3661 gtgatgatca aatttggcaa ttttgtgcca tgtttatatc cagtagactt gttggaacgg
     3721 ctgttgattg ttgataatat cgtccggttg gggatttacc gtcattttga gaaggagatt
     3781 aaagaagcgc tggattatgt ttaccgccat tggaatgagc gcgggattgg ttggggtcgg
     3841 ctaaatccca ttgcggactt agaaactacc gcattgggct ttcgcttgct acgtctgcat
     3901 cgttataatg tgtcccccgc gatttttgat aactttaaag atgccaatgg caagtttatt
     3961 tgcagcacag ggcagtttaa taaagatgtg gcgtccatgt taaatctcta ccgcgcctct
     4021 caattagcct ttcctggtga aaatattttg gacgaagcca aatcttttgc gacgaaatac
     4081 ctccgcgagg cgctcgaaaa gagcgaaacc tcctccgctt ggaataacaa acagaatctg
     4141 tcccaagaaa ttaaatatgc cttaaagacc tcctggcacg caagcgtacc tcgggttgaa
     4201 gctaagcgct attgccaggt gtaccgccct gattacgccc gcattgccaa gtgtgtttac
     4261 aaactcccct acgtcaacaa cgagaaattc ttggaactcg ggaaattaga cttcaatatt
     4321 attcagagta ttcatcaaga agaaatgaag aatgtaacca gttggtttcg tgacagtggt
     4381 ctacccttgt ttacctttgc ccgagaacgt cccctagagt tttactttct ggtggcagcg
     4441 ggtacctacg aaccccaata cgccaagtgc cggtttttgt ttaccaaagt ggcctgttta
     4501 caaaccgtcc tcgatgacat gtatgatacc tatggcacat tagatgaatt aaaattgttt
     4561 accgaagccg tccgtcggtg ggacctgagt tttaccgaaa atctgcccga ttacatgaag
     4621 ttatgctatc aaatttatta tgatattgtg cacgaagttg cctgggaagc cgaaaaagaa
     4681 caaggccgcg aattggtgag cttttttcgt aaagggtggg aggactatct attgggctac
     4741 tatgaagaag ctgagtggtt agccgccgaa tatgtgccca ccttagatga atatattaag
     4801 aatggcatca ccagcattgg acaacgcatt ttgttgttaa gcggcgtttt gattatggac
     4861 ggccaattac tatcccaaga ggctctcgaa aaagtcgact accccgggcg gcgtgttcta
     4921 actgaactca attctttaat ttcccggtta gccgacgata ccaaaacata caaagctgaa
     4981 aaagcccgtg gcgaattggc cagcagtatt gaatgttata tgaaagatca ccccgaatgt
     5041 acagaagaag aagctctgga tcacatctat agcatcttag aaccggccgt caaagagcta
     5101 acccgcgaat ttttaaagcc agacgatgtg ccctttgcgt gcaaaaaaat gctcttcgaa
     5161 gaaacccgtg ttactatggt gattttcaaa gatggtgatg gttttggagt gagcaagcta
     5221 gaagttaaag atcatatcaa ggagtgcttg attgaacctt tgcccctccg gtccggtagc
     5281 ggaagtgact ataaggatga tgatgacaag ggatagccgc ggccaggcat caaataaaac
     5341 gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc
     5401 tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat actgcaggag
     5461 cagaagagca tacatctgga agcaaagcca ggaaagcggc ctatggagct gtgcggcagc
     5521 gctcagtagg caatttttca aaatattgtt aagccttttc tgagcatggt atttttcatg
     5581 gtattaccaa ttagcaggaa aataagccat tgaatataaa agataaaaat gtcttgttta
     5641 caatagagtg gggggggtca gcctgccgcc ttgggccggg tgatgtcgta cttgcccgcc
     5701 gcgaactcgg ttaccgtcca gcccagcgcg accagctccg gcaacgcctc gcgcacccgc
     5761 tggcggcgct tgcgcatggt cgaaccactg gcctctgacg gccagacata gccgcacaag
     5821 gtatctatgg aagccttgcc ggttttgccg gggtcgatcc agccacacag ccgctggtgc
     5881 agcaggctgg cggtttcgct gtccagcgcc cgcacctcgt ccatgctgat gcgcacatgc
     5941 tggccgccac ccatgacggc ctgcgcgatc aaggggttca gggccacgta caggcgcccg
     6001 tccgcctcgt cgctggcgta ctccgacagc agccgaaacc cctgccgctt gcggccattc
     6061 tgggcgatga tggatacctt ccaaaggcgc tcgatgcagt cctgtatgtg cttgagcgcc
     6121 ccaccactat cgacctctgc cccgatttcc tttgccagcg cccgatagct acctttgacc
     6181 accatggcat cagcggtgac ggcctcccac ttgggttcca ggaacagccg gagctgccgt
     6241 ccgccttcgg tcttgggttc cgggccaagc actaggccat taggcccagc catggccacc
     6301 agcccttgca ggatgcgcag atcatcagcg cccagcggct ccgggccgct gaactcgatc
     6361 cgcttgccgt cgccgtagtc atacgtcacg tccagcttgc tgcgcttgcg ctcgccccgc
     6421 ttgagggcac ggaacaggcc gggggccaga cagtgcgccg ggtcgtgccg gacgtggctg
     6481 aggctgtgct tgttcttagg cttcaccacg gggcaccccc ttgctcttgc gctgcctctc
     6541 cagcacggcg ggcttgagca ccccgccgtc atgccgcctg aaccaccgat cagcgaacgg
     6601 tgcgccatag ttggccttgc tcacaccgaa gcggacgaag aaccggcgct ggtcgtcgtc
     6661 cacaccccat tcctcggcct cggcgctggt catgctcgac aggtaggact gccagcggat
     6721 gttatcgacc agtaccgagc tgccccggct ggcctgctgc tggtcgcctg cgcccatcat
     6781 ggccgcgccc ttgctggcat ggtgcaggaa cacgatagag cacccggtat cggcggcgat
     6841 ggcctccatg cgaccgatga cctgggccat ggggccgctg gcgttttctt cctcgatgtg
     6901 gaaccggcgc agcgtgtcca gcaccatcag gcggcggccc tcggcggcgc gcttgaggcc
     6961 gtcgaaccac tccggggcca tgatgttggg caggctgccg atcagcggct ggatcagcag
     7021 gccgtcagcc acggcttgcc gttcctcggc gctgaggtgc gccccaaggg cgtgcaggcg
     7081 gtgatgaatg gcggtgggcg ggtcttcggc gggcaggtag atcaccgggc cggtgggcag
     7141 ttcgcccacc tccagcagat ccggcccgcc tgcaatctgt gcggccagtt gcagggccag
     7201 catggattta ccggcaccac cgggcgacac cagcgccccg accgtaccgg ccaccatgtt
     7261 gggcaaaacg tagtccagcg gtggcggcgc tgctgcgaac gcctccagaa tattgatagg
     7321 cttatgggta gccattgatt gcctcctttg caggcagttg gtggttaggc gctggcgggg
     7381 tcactacccc cgccctgcgc cgctctgagt tcttccaggc actcgcgcag cgcctcgtat
     7441 tcgtcgtcgg tcagccagaa cttgcgctga cgcatccctt tggccttcat gcgctcggca
     7501 tatcgcgctt ggcgtacagc gtcagggctg gccagcaggt cgccggtctg cttgtccttt
     7561 tggtctttca tatcagtcac cgagaaactt gccggggccg aaaggcttgt cttcgcggaa
     7621 caaggacaag gtgcagccgt caaggttaag gctggccata tcagcgactg aaaagcggcc
     7681 agcctcggcc ttgtttgacg tataaccaaa gccaccgggc aaccaatagc ccttgtcact
     7741 tttgatcagg tagaccgacc ctgaagcgct tttttcgtat tccataaaac ccccttctgt
     7801 gcgtgagtac tcatagtata acaggcgtga gtaccaacgc aagcactaca tgctgaaatc
     7861 tggcccgccc ctgtccatgc ctcgctggcg gggtgccggt gcccgtgcca gctcggcccg
     7921 cgcaagctgg acgctgggca gacccatgac cttgctgacg gtgcgctcga tgtaatccgc
     7981 ttcgtggccg ggcttgcgct ctgccagcgc tgggctggcc tcggccatgg ccttgccgat
     8041 ttcctcggca ctgcggcccc ggctggccag cttctgcgcg gcgataaagt cgcacttgct
     8101 gaggtcatca ccgaagcgct tgaccagccc ggccatctcg ctgcggtact cgtccagcgc
     8161 cgtgcgccgg tggcggctaa gctgccgctc gggcagttcg aggctggcca gcctgcgggc
     8221 cttctcctgc tgccgctggg cctgctcgat ctgctggcca gcctgctgca ccagcgccgg
     8281 gccagcggtg gcggtcttgc ccttggattc acgcagcagc acccacggct gataaccggc
     8341 gcgggtggtg tgcttgtcct tgcggttggt gaagcccgcc aagcggccat agtggcggct
     8401 gtcggcgctg gccgggtcgg cgtcgtactc gctggccagc gtccgggcaa tctgcccccg
     8461 aagttcaccg cctgcggcgt cggccacctt gacccatgcc tgatagttct tcgggctggt
     8521 ttccactacc agggcaggct cccggccctc ggctttcatg tcatccaggt caaactcgct
     8581 gaggtcgtcc accagcacca gaccatgccg ctcctgctcg gcgggcctga tatacacgtc
     8641 attgccctgg gcattcatcc gcttgagcca tggcgtgttc tggagcactt cggcggctga
     8701 ccattcccgg ttcatcatct ggccggtggt ggcgtccctg acgccgatat cgaagcgctc
     8761 acagcccatg gccttgagct gtcggcctat ggcctgcaaa gtcctgtcgt tcttcatcgg
     8821 gccaccaagc gcagccagat cgagccgtcc tcggttgtca gtggcgtcag gtcgagcaag
     8881 agcaacgatg cgatcagcag caccaccgta ggcatcatgg aagccagcat cacggttagc
     8941 catagcttcc agtgccaccc ccgcgacgcg ctccgggcgc tctgcgcggc gctgctcacc
     9001 tcggcggcta cctcccgcaa ctctttggcc agctccaccc atgccgcccc tgtctggcgc
     9061 tgggctttca gccactccgc cgcctgcgcc tcgctggcct gctgggtctg gctcatgacc
     9121 tgccgggctt cgtcggccag tgtcgccatg ctctgggcca gcggttcgat ctgctccgct
     9181 aactcgttga tgcctctgga tttcttcact ctgtcgattg cgttcatggt ctattgcctc
     9241 ccggtattcc tgtaagtcga tgatctgggc gttggcggtg tcgatgttca gggccacgtc
     9301 tgcccggtcg gtgcggatgc cccggccttc catctccacc acgttcggcc ccaggtgaac
     9361 accgggcagg cgctcgatgc cctgcgcctc aagtgttctg tggtcaatgc gggcgtcgtg
     9421 gccagcccgc tctaatgccc ggttggcatg gtcggcccat gcctcgcggg tctgctcaag
     9481 ccatgccttg ggcttgagcg cttcggtctt ctgtgccccg cccttctccg gggtcttgcc
     9541 gttgtaccgc ttgaaccact gagcggcggg ccgctcgatg ccgtcattga tccgctcgga
     9601 gatcatcagg tggcagtgcg ggttctcgcc gccaccggca tggatggcca gcgtatacgg
     9661 caggcgctcg gcaccggtca ggtgctgggc gaactcggac gccagcgcct tctgctggtc
     9721 gagggtcagc tcgaccggca gggcaaattc gacctccttg aacagccgcc cattggcgcg
     9781 ttcatacagg tcggcagcat cccagtagtc ggcgggccgc tcgacgaact ccggcatgtg
     9841 cccggattcg gcgtgcaaga cttcatccat gtcgcgggca tacttgcctt cgcgctggat
     9901 gtagtcggcc ttggccctgg ccgattggcc gcccgacctg ctgccggttt tcgccgtaag
     9961 gtgataaatc gccatgctgc ctcgctgttg cttttgcttt tcggctccat gcaatggccc
    10021 tcggagagcg caccgcccga agggtggccg ttaggccagt ttctcgaaga gaaaccggta
    10081 agtgcgccct cccctacaaa gtagggtcgg gattgccgcc gctgtgcctc catgatagcc
    10141 tacgagacag cacattaaca atggggtgtc aagatggtta aggggagcaa caaggcggcg
    10201 gatcggctgg ccaagctcga agaacaacga gcgcgaatca atgccgaaat tcagcgggtg
    10261 cgggcaaggg aacagcagca agagcgcaag aacgaaacaa ggcgcaaggt gctggtgggg
    10321 gccatgattt tggccaaggt gaacagcagc gagtggccgg aggatcggct catggcggca
    10381 atggatgcgt accttgaacg cgaccacgac cgcgccttgt tcggtctgcc gccacgccag
    10441 aaggatgagc cgggctgaat gatcgaccga gacaggccct gcggggctgc acacgcgccc
    10501 ccacccttcg ggtaggggga aaggccgcta aagcggctaa aagcgctcca gcgtatttct
    10561 gcggggtttg gtgtggggtt tagcgggctt tgcccgcctt tccccctgcc gcgcagcggt
    10621 ggggcggtgt gtagcctagc gcagcgaata gaccagctat ccggcctctg gccgggcata
    10681 ttgggcaagg gcagcagcgc cccacaaggg cgctgataac cgcgcctagt ggattattct
    10741 tagataatca tggatggatt tttccaacac cccgccagcc cccgcccctg ctgggtttgc
    10801 aggtttgggg gcgtgacagt tattgcaggg gttcgtgaca gttattgcag gggggcgtga
    10861 cagttattgc aggggttcgt gacagttagt acgggagtga cggg
//
