LOCUS       sequence-271353-        6685 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Retroviral plasmid expressing GFP - Nde1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6685)
  AUTHORS   Monda JK, Cheeseman IM
  TITLE     Nde1 promotes diverse dynein functions through differential 
            interactions and exhibits an isoform-specific proteasome 
            association.
  JOURNAL   Mol Biol Cell. 2018 Sep 15;29(19):2336-2345. doi: 
            10.1091/mbc.E18-07-0418. Epub 2018 Jul 19.
  PUBMED    30024347
REFERENCE   2  (bases 1 to 6685)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E18-07-0418"; journalName: "Mol Biol Cell"; date: 
            "2018-09-15- 15"; volume: "29"; issue: "19"; pages: "2336-2345"
COMMENT     Sequence Label: pJM007
FEATURES             Location/Qualifiers
     source          1..6685
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             320..736
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     666..688
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     704..720
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     regulatory      771..780
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(777..779,780..782,783..1493)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(777..779,780..782,783..1493)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(822..843)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1083..1102)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1430..1451
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             1539..1559
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1572..1616
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             2327..2338
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     primer_bind     complement(2648..2668)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2653..2982
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2833..2968
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2990..3388
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             3557..4026
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(4156..4173)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4327..4915)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4407..4426)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5075..5866,5867..5935))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5698..5717
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5936..6040)
                     /gene="bla"
                     /label=AmpR promoter
     LTR             6071..6540
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    join(6605..6685,1..119)
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
ORIGIN
        1 agctaactag ctctgtatct ggcggacccg tggtggaact gacgagttct gaacacccgg
       61 ccgcaaccct gggagacgtc ccagggactt tgggggccgt ttttgtggcc cgacctgagg
      121 aagggagtcg atgtggaatc cgaccccgtc aggatatgtg gttctggtag gagacgagaa
      181 cctaaaacag ttcccgcctc cgtctgaatt tttgctttcg gtttggaacc gaagccgcgc
      241 gtcttgtctg ctgcagcgct gcagcatcgt tctgtgttgt ctctgtctga ctgtgtttct
      301 gtatttgtct gaaaattagg gccagactgt taccactccc ttaagtttga ccttaggtca
      361 ctggaaagat gtcgagcgga tcgctcacaa ccagtcggta gatgtcaaga agagacgttg
      421 ggttaccttc tgctctgcag aatggccaac ctttaacgtc ggatggccgc gagacggcac
      481 ctttaaccga gacctcatca cccaggttaa gatcaaggtc ttttcacctg gcccgcatgg
      541 acacccagac caggtcccct acatcgtgac ctgggaagcc ttggcttttg acccccctcc
      601 ctgggtcaag ccctttgtac accctaagcc tccgcctcct cttcctccat ccgccccgtc
      661 tctccccctt gaacctcctc gttcgacccc gcctcgatcc tccctttatc cagccctcac
      721 tccttctcta ggcgccggcc ggatcccagt gtggtggtac gctaccggtc gccaccatgg
      781 tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg
      841 acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca
      901 agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg
      961 tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc
     1021 acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca
     1081 aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga
     1141 accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc
     1201 tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca
     1261 tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc
     1321 actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc
     1381 tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc
     1441 tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtccggag
     1501 gggatcggtg gagctccacc ggtggcggcc gctctagaga gaatctttat tttcagggcg
     1561 ccgccaaatt caaagaaacc gctgctgcta aattcgaacg ccagcacatg gacagcggag
     1621 gtggaggtac tagtctcgag atggaggact ccggaaagac tttcagctcc gaggaggaag
     1681 aagctaacta ttggaaagat ctggcgatga cctacaaaca gagggcagaa aatacgcaag
     1741 aggaactccg agaattccag gagggaagcc gagaatatga agctgaattg gagacgcagc
     1801 tgcaacaaat tgaaaccagg aacagagacc tcctgtccga aaataaccgc cttcgcatgg
     1861 agctggaaac catcaaggag aagtttgaag tgcagcactc tgaaggctac cggcagatct
     1921 cagccttgga ggatgacctc gcgcagacca aagccattaa agaccaattg cagaaataca
     1981 tcagagagct ggagcaagca aatgacgacc tggaaagagc caagcgcgcc acgatcatgt
     2041 ctctcgaaga ctttgagcag cgcttgaatc aggccatcga aagaaatgcc ttcctggaaa
     2101 gtgaacttga tgaaaaagag aatctcctgg aatctgttca gagactgaag gatgaagcca
     2161 gagatttgcg gcaggaactg gccgtgcagc agaagcagga gaaacccagg acccccatgc
     2221 ccagctcagt ggaagctgag aggacagaca cagctgtgca ggccacgggc tccgtgccgt
     2281 ccacgcccat tgctcaccga ggaccaagct caagtttaaa cacacctggg agcttcagac
     2341 gtggcctgga cgactccacc ggggggaccc ccctcacacc tgcggcccgg atatcagccc
     2401 tcaacattgt gggagaccta ctgcggaaag tcggggcact ggagtccaaa ctcgcttcct
     2461 gccggaacct cgtgtacgat cagtccccat gaccgcgggc ccgggatcca ccggatctag
     2521 ataactgatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct
     2581 cccacacctc cccctgaacc tgaaacataa aatgaatgca attcgccagc acagtggtcg
     2641 accctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga
     2701 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
     2761 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc
     2821 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     2881 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
     2941 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagcttca tggccaagcc
     3001 tttgtctcaa gaagaatcca ccctcattga aagagcaacg gctacaatca acagcatccc
     3061 catctctgaa gactacagcg tcgccagcgc agctctctct agcgacggcc gcatcttcac
     3121 tggtgtcaat gtatatcatt ttactggggg accttgtgca gaactcgtgg tgctgggcac
     3181 tgctgctgct gcggcagctg gcaacctgac ttgtatcgtc gcgatcggaa atgagaacag
     3241 gggcatcttg agcccctgcg gacggtgccg acaggtgctt ctcgatctgc atcctgggat
     3301 caaagccata gtgaaggaca gtgatggaca gccgacggca gttgggattc gtgaattgct
     3361 gccctctggt tatgtgtggg agggctaaga tcgataaaat aaaagatttt atttagtctc
     3421 cagaaaaagg ggggaatgaa agaccccacc tgtaggtttg gcaagctagc ttaagtaacg
     3481 ccattttgca aggcatggaa aaatacataa ctgagaatag agaagttcag atcaaggtca
     3541 ggaacagatg gaacagctga atatgggcca aacaggatat ctgtggtaag cagttcctgc
     3601 cccggctcag ggccaagaac agatggaaca gctgaatatg ggccaaacag gatatctgtg
     3661 gtaagcagtt cctgccccgg ctcagggcca agaacagatg gtccccagat gcggtccagc
     3721 cctcagcagt ttctagagaa ccatcagatg tttccagggt gccccaagga cctgaaatga
     3781 ccctgtgcct tatttgaact aaccaatcag ttcgcttctc gcttctgttc gcgcgcttct
     3841 gctccccgag ctcaataaaa gagcccacaa cccctcactc ggggcgccag tcctccgatt
     3901 gactgagtcg cccgggtacc cgtgtatcca ataaaccctc ttgcagttgc atccgacttg
     3961 tggtctcgct gttccttggg agggtctcct ctgagtgatt gactacccgt cagcgggggt
     4021 ctttcacatg cagcatgtat caaaattaat ttggtttttt ttcttaagta tttacattaa
     4081 atggccatag ttgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     4141 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     4201 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     4261 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     4321 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     4381 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     4441 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     4501 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     4561 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     4621 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     4681 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     4741 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     4801 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     4861 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     4921 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     4981 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttgcggc cgcaaatcaa
     5041 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     5101 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     5161 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     5221 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     5281 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     5341 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     5401 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     5461 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     5521 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     5581 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     5641 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     5701 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     5761 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     5821 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     5881 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     5941 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     6001 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     6061 cacctgcagc ctgaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc
     6121 tcagggccaa gaacagatgg aacagctgaa tatgggccaa acaggatatc tgtggtaagc
     6181 agttcctgcc ccggctcagg gccaagaaca gatggtcccc agatgcggtc cagccctcag
     6241 cagtttctag agaaccatca gatgtttcca gggtgcccca aggacctgaa atgaccctgt
     6301 gccttatttg aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc ttctgctccc
     6361 cgagctcaat aaaagagccc acaacccctc actcggggcg ccagtcctcc gattgactga
     6421 gtcgcccggg tacccgtgta tccaataaac cctcttgcag ttgcatccga cttgtggtct
     6481 cgctgttcct tgggagggtc tcctctgagt gattgactac ccgtcagcgg gggtctttca
     6541 tttgggggct cgtccgggat cgggagaccc ctgcccaggg accaccgacc caccaccggg
     6601 aggcaagctg gccagcaact tatctgtgtc tgtccgattg tctagtgtct atgactgatt
     6661 ttatgcgcct gcgtcggtac tagtt
//
