LOCUS       sequence-271360-        9318 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentivirus for base editing activatable GFP expression in mammalian 
            cells. All-in-one vector with sgGO2. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9318)
  AUTHORS   Katti A, Foronda M, Zimmerman J, Diaz B, Zafra MP, Goswami S, Dow LE
  TITLE     GO: a functional reporter system to identify and enrich base editing
            activity.
  JOURNAL   Nucleic Acids Res. 2020 Feb 29. pii: 5766653. doi: 
            10.1093/nar/gkaa124.
  PUBMED    32112097
REFERENCE   2  (bases 1 to 9318)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Feb 29. pii: 5766653. doi: 10.1093/nar/gkaa124."
COMMENT     Sequence Label: pRRL-mU6-GFPGO-IRES-mScarletI
FEATURES             Location/Qualifiers
     source          1..9318
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1806..1923
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1982..2295
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for mouse U6 snRNA (Das 
                     et al., 1988)"
     primer_bind     2214..2234
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     CDS             join(2855..2857,2858..3571)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(2855..2857,2858..3568)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(2897..2918)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3158..3177)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3505..3526
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    3659..4211
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3803..3820)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4030..4049
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4235..4255
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4259..4279
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4283..4303
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     4917..4940
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     misc_feature    5046..5634
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5099..5119)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5517..5528)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5841..6074
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6146..6267
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6183..6202)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6237..6256
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6307..6442
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6462..6481)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6463..6481)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6491..6508)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6491..6507)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6500..6522)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6649..7104
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6736..6755)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6946..6967
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7130..7234
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7235..7303,7304..8095)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7453..7472)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8266..8854
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8755..8774
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9008..9025
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9142..9163
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9178..9183,9184..9201,9202..9208)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9216..9232
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9221..9243
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9240..9256
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9240..9256
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9275..9295
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9277..9295
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
     1741 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcgg
     1801 ttaactttta aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac
     1861 ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat
     1921 tttatcgatc acgagactag cctcgatcgc aattctgcag acaaatggct ctagatctag
     1981 agatccgacg ccgccatctc taggcccgcg ccggccccct cgcacagact tgtgggagaa
     2041 gctcggctac tcccctgccc cggttaattt gcatataata tttcctagta actatagagg
     2101 cttaatgtgc gataaaagac agataatctg ttctttttaa tactagctac attttacatg
     2161 ataggcttgg atttctataa gagatacaaa tactaaatta ttattttaaa aaacagcaca
     2221 aaaggaaact caccctaact gtaaagtaat tgtgtgtttt gagactataa atatgcatgc
     2281 gagaaaagcc ttgtttgacg acggtgagca agggcggttt aagagctatg ctggaaacag
     2341 catagcaagt ttaaataagg ctagtccgtt atcaacttga aaaagtggca ccgagtcggt
     2401 gcttttttgt tttaagtctc gagatcgagg gctagcgact agctgcagta acgccatttt
     2461 gcaaggcatg gaaaaatacc aaaccaagaa tagagaagtt cagatcaagg gcgggtacat
     2521 gaaaatagct aacgttgggc caaacaggat atctgcggtg agcagtttcg gccccggccc
     2581 ggggccaaga acagatggtc accgcagttt cggccccggc ccgaggccaa gaacagatgg
     2641 tccccagata tggcccaacc ctcagcagtt tcttaagacc catcagatgt ttccaggctc
     2701 ccccaaggac ctgaaatgac cctgcgcctt atttgaatta accaatcagc ctgcttctcg
     2761 cttctgttcg cgcgcttctg cttcccgagc tctataaaag agctcacaac ccctcactcg
     2821 gcgcgccagt cctccgacag aattcgcgac gacggtgagc aagggcgagg agctgttcac
     2881 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
     2941 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac
     3001 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca
     3061 gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
     3121 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg
     3181 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga
     3241 cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa
     3301 cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca
     3361 caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg
     3421 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa
     3481 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat
     3541 cactctcggc atggacgagc tgtacaagta atgacgtctc cccgggtaat ccggactcga
     3601 cctgcagcca agcttatcac gcgttgagaa cttcagggtg agtttgggcc cccccctaac
     3661 gttactggcc gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat gttattttcc
     3721 accatattgc cgtcttttgg caatgtgagg gcccggaaac ctggccctgt cttcttgacg
     3781 agcattccta ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg
     3841 aaggaagcag ttcctctgga agcttcttga agacaaacaa cgtctgtagc gaccctttgc
     3901 aggcagcgga accccccacc tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa
     3961 gatacacctg caaaggcggc acaaccccag tgccacgttg tgagttggat agttgtggaa
     4021 agagtcaaat ggctctcctc aagcgtattc aacaaggggc tgaaggatgc ccagaaggta
     4081 ccccattgta tgggatctga tctggggcct cggtgcacat gctttacatg tgtttagtcg
     4141 aggttaaaaa aacgtctagg ccccccgaac cacggggacg tggttttcct ttgaaaaaca
     4201 cgatgataat atggccacaa ccatgggcag cgacccaaag aagaagcgga aagtggaccc
     4261 caagaaaaag agaaaggtgg accccaaaaa gaagcggaag gtgggcagcc ccgtggccac
     4321 aatggtgagc aagggcgagg ccgtgatcaa ggagttcatg aggttcaagg tgcacatgga
     4381 gggctccatg aacggtcacg agttcgaaat cgagggcgaa ggcgagggca ggccctacga
     4441 gggcacccag accgccaagc tgaaggtgac aaagggcggc cccctgccct tctcctggga
     4501 catcctgtcc cctcagttca tgtacggctc cagggccttc atcaagcacc ccgccgacat
     4561 ccccgactac tacaagcagt ccttccccga gggcttcaag tgggagaggg tgatgaactt
     4621 cgaggacggc ggcgccgtga ccgtgaccca ggacacctcc ctggaggacg gcaccctgat
     4681 ctacaaggtg aagctgaggg gcaccaactt ccctcctgac ggccccgtga tgcagaagaa
     4741 gacaatgggc tgggaggcct ccaccgagag gctgtacccc gaggacggcg tgctgaaggg
     4801 cgacatcaag atggccctga ggctgaagga cggcggcaga tacctggccg acttcaagac
     4861 cacctacaag gccaagaagc ccgtgcagat gcccggcgcc tacaacgtgg acaggaagct
     4921 ggacatcacc tcccacaacg aggactacac cgtggtggag cagtacgaga ggtccgaggg
     4981 caggcactcc accggcggca tggacgagct gtataagtga gtcgacttcg aaacgcgtct
     5041 ggaacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
     5101 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
     5161 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
     5221 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
     5281 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
     5341 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
     5401 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc
     5461 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
     5521 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
     5581 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctggaa
     5641 ttaattctgc agtcgagacc tagaaaaaca tggagcaatc acaagtagca atacagcagc
     5701 taccaatgct gattgtgcct ggctagaagc acaagaggag gaggaggtgg gttttccagt
     5761 cacacctcag gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt
     5821 tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac aagatctgct
     5881 ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     5941 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     6001 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     6061 gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa
     6121 gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata
     6181 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     6241 tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc gcccctaact
     6301 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     6361 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag
     6421 tgaggaggct tttttggagg cctagggacg tacccaattc gccctatagt gagtcgtatt
     6481 acgcgcgctc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc
     6541 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc
     6601 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgggac gcgccctgta
     6661 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
     6721 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
     6781 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
     6841 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat
     6901 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
     6961 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc
     7021 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta
     7081 acaaaatatt aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     7141 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     7201 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     7261 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     7321 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     7381 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     7441 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
     7501 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     7561 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     7621 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     7681 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     7741 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     7801 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     7861 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     7921 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     7981 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     8041 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     8101 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     8161 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     8221 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     8281 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     8341 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     8401 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     8461 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     8521 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     8581 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     8641 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     8701 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     8761 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     8821 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     8881 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     8941 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     9001 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct
     9061 ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc
     9121 gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt
     9181 acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac
     9241 aggaaacagc tatgaccatg attacgccaa gcgcgcaatt aaccctcact aaagggaaca
     9301 aaagctggag ctgcaagc
//
