LOCUS       sequence-271366-        6049 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Retroviral plasmid expressing GFP - Nde1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6049)
  AUTHORS   Monda JK, Cheeseman IM
  TITLE     Nde1 promotes diverse dynein functions through differential 
            interactions and exhibits an isoform-specific proteasome 
            association.
  JOURNAL   Mol Biol Cell. 2018 Sep 15;29(19):2336-2345. doi: 
            10.1091/mbc.E18-07-0418. Epub 2018 Jul 19.
  PUBMED    30024347
REFERENCE   2  (bases 1 to 6049)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E18-07-0418"; journalName: "Mol Biol Cell"; date: 
            "2018-09-15- 15"; volume: "29"; issue: "19"; pages: "2336-2345"
COMMENT     Sequence Label: pJM011
FEATURES             Location/Qualifiers
     source          1..6049
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             64..480
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     410..432
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     448..464
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     regulatory      515..524
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(521..523,524..526,527..1237)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(521..523,524..526,527..1237)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(566..587)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(827..846)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1174..1195
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             1283..1303
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1316..1360
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     primer_bind     complement(1756..1776)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        1761..2090
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      1941..2076
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2098..2496
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             2665..3134
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(3264..3281)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3435..4023)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3515..3534)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4183..4974,4975..5043))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4806..4825
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5044..5148)
                     /gene="bla"
                     /label=AmpR promoter
     LTR             5179..5648
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    5713..5912
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
ORIGIN
        1 cgctgcagca tcgttctgtg ttgtctctgt ctgactgtgt ttctgtattt gtctgaaaat
       61 tagggccaga ctgttaccac tcccttaagt ttgaccttag gtcactggaa agatgtcgag
      121 cggatcgctc acaaccagtc ggtagatgtc aagaagagac gttgggttac cttctgctct
      181 gcagaatggc caacctttaa cgtcggatgg ccgcgagacg gcacctttaa ccgagacctc
      241 atcacccagg ttaagatcaa ggtcttttca cctggcccgc atggacaccc agaccaggtc
      301 ccctacatcg tgacctggga agccttggct tttgaccccc ctccctgggt caagcccttt
      361 gtacacccta agcctccgcc tcctcttcct ccatccgccc cgtctctccc ccttgaacct
      421 cctcgttcga ccccgcctcg atcctccctt tatccagccc tcactccttc tctaggcgcc
      481 ggccggatcc cagtgtggtg gtacgctacc ggtcgccacc atggtgagca agggcgagga
      541 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa
      601 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt
      661 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta
      721 cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc
      781 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta
      841 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa
      901 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa
      961 cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa
     1021 gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac
     1081 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc
     1141 cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc
     1201 cgccgggatc actctcggca tggacgagct gtacaagtcc ggaggggatc ggtggagctc
     1261 caccggtggc ggccgctcta gagagaatct ttattttcag ggcgccgcca aattcaaaga
     1321 aaccgctgct gctaaattcg aacgccagca catggacagc ggaggtggag gtactagtct
     1381 cgagatggca ctggagtcca aactcgcttc ctgccggaac ctcgtgtacg atcagtcccc
     1441 aaaccgaaca ggtggcccag cctctgggcg gagcagcaag aacagagatg gcggggagag
     1501 acggccaagc agcaccagcg tgcctttggg tgataagggg ttggacacga gttgccgctg
     1561 gttgtccaaa tcaacaacca ggtcgtccag ctcctgctga ccgcgggccc gggatccacc
     1621 ggatctagat aactgatcat aatcagccat accacatttg tagaggtttt acttgcttta
     1681 aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat tcgccagcac
     1741 agtggtcgac cctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
     1801 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
     1861 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
     1921 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     1981 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
     2041 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagcttcatg
     2101 gccaagcctt tgtctcaaga agaatccacc ctcattgaaa gagcaacggc tacaatcaac
     2161 agcatcccca tctctgaaga ctacagcgtc gccagcgcag ctctctctag cgacggccgc
     2221 atcttcactg gtgtcaatgt atatcatttt actgggggac cttgtgcaga actcgtggtg
     2281 ctgggcactg ctgctgctgc ggcagctggc aacctgactt gtatcgtcgc gatcggaaat
     2341 gagaacaggg gcatcttgag cccctgcgga cggtgccgac aggtgcttct cgatctgcat
     2401 cctgggatca aagccatagt gaaggacagt gatggacagc cgacggcagt tgggattcgt
     2461 gaattgctgc cctctggtta tgtgtgggag ggctaagatc gataaaataa aagattttat
     2521 ttagtctcca gaaaaagggg ggaatgaaag accccacctg taggtttggc aagctagctt
     2581 aagtaacgcc attttgcaag gcatggaaaa atacataact gagaatagag aagttcagat
     2641 caaggtcagg aacagatgga acagctgaat atgggccaaa caggatatct gtggtaagca
     2701 gttcctgccc cggctcaggg ccaagaacag atggaacagc tgaatatggg ccaaacagga
     2761 tatctgtggt aagcagttcc tgccccggct cagggccaag aacagatggt ccccagatgc
     2821 ggtccagccc tcagcagttt ctagagaacc atcagatgtt tccagggtgc cccaaggacc
     2881 tgaaatgacc ctgtgcctta tttgaactaa ccaatcagtt cgcttctcgc ttctgttcgc
     2941 gcgcttctgc tccccgagct caataaaaga gcccacaacc cctcactcgg ggcgccagtc
     3001 ctccgattga ctgagtcgcc cgggtacccg tgtatccaat aaaccctctt gcagttgcat
     3061 ccgacttgtg gtctcgctgt tccttgggag ggtctcctct gagtgattga ctacccgtca
     3121 gcgggggtct ttcacatgca gcatgtatca aaattaattt ggtttttttt cttaagtatt
     3181 tacattaaat ggccatagtt gcattaatga atcggccaac gcgcggggag aggcggtttg
     3241 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     3301 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     3361 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     3421 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     3481 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     3541 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     3601 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     3661 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     3721 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     3781 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     3841 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     3901 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     3961 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     4021 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     4081 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttgcggccg
     4141 caaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     4201 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     4261 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     4321 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     4381 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     4441 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     4501 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     4561 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     4621 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     4681 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     4741 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     4801 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     4861 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     4921 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     4981 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     5041 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     5101 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     5161 aaaagtgcca cctgcagcct gaatatgggc caaacaggat atctgtggta agcagttcct
     5221 gccccggctc agggccaaga acagatggaa cagctgaata tgggccaaac aggatatctg
     5281 tggtaagcag ttcctgcccc ggctcagggc caagaacaga tggtccccag atgcggtcca
     5341 gccctcagca gtttctagag aaccatcaga tgtttccagg gtgccccaag gacctgaaat
     5401 gaccctgtgc cttatttgaa ctaaccaatc agttcgcttc tcgcttctgt tcgcgcgctt
     5461 ctgctccccg agctcaataa aagagcccac aacccctcac tcggggcgcc agtcctccga
     5521 ttgactgagt cgcccgggta cccgtgtatc caataaaccc tcttgcagtt gcatccgact
     5581 tgtggtctcg ctgttccttg ggagggtctc ctctgagtga ttgactaccc gtcagcgggg
     5641 gtctttcatt tgggggctcg tccgggatcg ggagacccct gcccagggac caccgaccca
     5701 ccaccgggag gcaagctggc cagcaactta tctgtgtctg tccgattgtc tagtgtctat
     5761 gactgatttt atgcgcctgc gtcggtacta gttagctaac tagctctgta tctggcggac
     5821 ccgtggtgga actgacgagt tctgaacacc cggccgcaac cctgggagac gtcccaggga
     5881 ctttgggggc cgtttttgtg gcccgacctg aggaagggag tcgatgtgga atccgacccc
     5941 gtcaggatat gtggttctgg taggagacga gaacctaaaa cagttcccgc ctccgtctga
     6001 atttttgctt tcggtttgga accgaagccg cgcgtcttgt ctgctgcag
//
