LOCUS       sequence-27137-e         768 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 768)
  AUTHORS   Behm-Ansmant I, Rehwinkel J, Doerks T, Stark A, Bork P, Izaurralde E
  TITLE     mRNA degradation by miRNAs and GW182 requires both CCR4:NOT 
            deadenylase and DCP1:DCP2 decapping complexes.
  JOURNAL   Genes Dev. 2006 Jul 15. 20(14):1885-98.
  PUBMED    16815998
REFERENCE   2  (bases 1 to 768)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            2006 Jul 15. 20(14):1885-98."
COMMENT     Sequence Label: pAc5.1B-lambdaN-HA
FEATURES             Location/Qualifiers
     source          1..768
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             87..113
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             186..227
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             237..254
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(277..294)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    327..461
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(351..370)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     405..424
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 acgcacaaac acgacgacgt gagcgtcgcg ctgagaaaca agctcaatgg aaagctgcaa
       61 acggtggatc tcctccacat atggcttacc catacgatgt tccagattac gctcctccaa
      121 gcttgaattc tgcagatatc cagcacagtg gcggccgctc gagtctagag ggcccgcggt
      181 tcgaaggtaa gcctatccct aaccctctcc tcggtctcga ttctacgcgt accggtcatc
      241 atcaccatca ccattgagtt taaacccgct gatcagcctc gactgtgcct tctaaggcct
      301 gagctcgctg atcagcctcg atcgaggatc cagacatgat aagatacatt gatgagtttg
      361 gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta
      421 ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc
      481 attttatgtt tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag taaaacctct
      541 acaaatgtgg tatggctgat tatgatcagt cgaccgatgc ccttgagagc cttcaaccca
      601 gtcagctcct tccggtgggc gcggggcatg actatcgtcg ccgcacttat gactgtcttc
      661 tttatcatgc anctcgtagg acaggtgccg gcagcgctct gggtcatttt cggcgaggac
      721 cgctttcgct ggagcgcgac gatgatcggc ctgtcgcttg cggtattc
//
