LOCUS       sequence-271371-        6688 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Retroviral plasmid expressing GFP PSMD14.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6688)
  AUTHORS   Monda JK, Cheeseman IM
  TITLE     Nde1 promotes diverse dynein functions through differential 
            interactions and exhibits an isoform-specific proteasome 
            association.
  JOURNAL   Mol Biol Cell. 2018 Sep 15;29(19):2336-2345. doi: 
            10.1091/mbc.E18-07-0418. Epub 2018 Jul 19.
  PUBMED    30024347
REFERENCE   2  (bases 1 to 6688)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E18-07-0418"; journalName: "Mol Biol Cell"; date: 
            "2018-09-15- 15"; volume: "29"; issue: "19"; pages: "2336-2345"
COMMENT     Sequence Label: pJM014
FEATURES             Location/Qualifiers
     source          1..6688
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             71..487
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     417..439
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     455..471
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     regulatory      503..512
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1454..1471
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1481..1504
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             1505..1528
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             join(1538..1540,1541..1543,1544..2257)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTATGITLGMDELYK"
     primer_bind     complement(1583..1604)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1844..1863)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2191..2212
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(2402..2422)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2407..2736
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2587..2722
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2744..3142
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             3311..3780
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(3910..3927)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4081..4669)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4161..4180)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4829..5620,5621..5689))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5452..5471
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5690..5794)
                     /gene="bla"
                     /label=AmpR promoter
     LTR             5825..6294
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    6359..6558
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
ORIGIN
        1 gctgcagcgc tgcagcatcg ttctgtgttg tctctgtctg actgtgtttc tgtatttgtc
       61 tgaaaattag ggccagactg ttaccactcc cttaagtttg accttaggtc actggaaaga
      121 tgtcgagcgg atcgctcaca accagtcggt agatgtcaag aagagacgtt gggttacctt
      181 ctgctctgca gaatggccaa cctttaacgt cggatggccg cgagacggca cctttaaccg
      241 agacctcatc acccaggtta agatcaaggt cttttcacct ggcccgcatg gacacccaga
      301 ccaggtcccc tacatcgtga cctgggaagc cttggctttt gacccccctc cctgggtcaa
      361 gccctttgta caccctaagc ctccgcctcc tcttcctcca tccgccccgt ctctccccct
      421 tgaacctcct cgttcgaccc cgcctcgatc ctccctttat ccagccctca ctccttctct
      481 aggcgccggc cggatcctcg aggccaccat ggacagactt cttagacttg gaggaggtat
      541 gcctggactg ggccaggggc cacctacaga tgctcctgca gtggacacag cagaacaagt
      601 ctatatctct tccctggcac tgttaaaaat gttaaaacat ggccgtgctg gagttccaat
      661 ggaagttatg ggtttgatgc ttggagaatt tgttgatgat tataccgtca gagtgattga
      721 tgtgtttgct atgccacagt caggaacagg tgtcagtgtg gaggcagttg atccagtgtt
      781 ccaagctaaa atgttggata tgttgaagca gacaggaagg ccggagatgg ttgttggttg
      841 gtatcacagt caccctggct ttggttgttg gctttctggt gtggatatca acactcagca
      901 gagctttgaa gccttgtcgg agagagctgt ggcagtggtt gtggatccca ttcagagtgt
      961 aaaaggaaag gttgttattg atgccttcag attgatcaat gctaatatga tggtcttagg
     1021 acatgaacca agacaaacaa cttcgaatct gggtcactta aacaagccat ctatccaggc
     1081 attaattcat ggactaaaca gacattatta ctccattact attaactatc ggaaaaatga
     1141 actggaacag aagatgttgc taaatttgca taagaagagt tggatggaag gtttgacact
     1201 tcaggactac agtgaacatt gtaaacacaa tgaatcagtg gtaaaggaga tgttggaatt
     1261 agccaagaat tacaataagg ctgtagaaga agaagataag atgacacctg aacagctggc
     1321 aataaagaat gttggcaagc aggaccccaa acgtcatttg gaggaacatg tggatgtact
     1381 tatgacctca aatattgtcc agtgtttagc agctatgttg gatactgtcg tatttaaagg
     1441 ccgcggcagc agccatcatc atcatcatca cagcagcggc ctggaagttc tgttccaggg
     1501 gcccctggaa gttctgttcc aggggccctc cggagggatg gtgagcaagg gcgaggagct
     1561 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
     1621 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat
     1681 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg
     1741 cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc
     1801 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
     1861 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
     1921 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag
     1981 ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat
     2041 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc
     2101 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct
     2161 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccac
     2221 cgggatcact cttggcatgg acgagctgta caagtaaaga tccaccggat ctagataact
     2281 gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca
     2341 cctccccctg aacctgaaac ataaaatgaa tgcaattcgc cagcacagtg gtcgaccctg
     2401 tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     2461 caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca
     2521 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact
     2581 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     2641 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag
     2701 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc ttcatggcca agcctttgtc
     2761 tcaagaagaa tccaccctca ttgaaagagc aacggctaca atcaacagca tccccatctc
     2821 tgaagactac agcgtcgcca gcgcagctct ctctagcgac ggccgcatct tcactggtgt
     2881 caatgtatat cattttactg ggggaccttg tgcagaactc gtggtgctgg gcactgctgc
     2941 tgctgcggca gctggcaacc tgacttgtat cgtcgcgatc ggaaatgaga acaggggcat
     3001 cttgagcccc tgcggacggt gccgacaggt gcttctcgat ctgcatcctg ggatcaaagc
     3061 catagtgaag gacagtgatg gacagccgac ggcagttggg attcgtgaat tgctgccctc
     3121 tggttatgtg tgggagggct aagatcgata aaataaaaga ttttatttag tctccagaaa
     3181 aaggggggaa tgaaagaccc cacctgtagg tttggcaagc tagcttaagt aacgccattt
     3241 tgcaaggcat ggaaaaatac ataactgaga atagagaagt tcagatcaag gtcaggaaca
     3301 gatggaacag ctgaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc
     3361 tcagggccaa gaacagatgg aacagctgaa tatgggccaa acaggatatc tgtggtaagc
     3421 agttcctgcc ccggctcagg gccaagaaca gatggtcccc agatgcggtc cagccctcag
     3481 cagtttctag agaaccatca gatgtttcca gggtgcccca aggacctgaa atgaccctgt
     3541 gccttatttg aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc ttctgctccc
     3601 cgagctcaat aaaagagccc acaacccctc actcggggcg ccagtcctcc gattgactga
     3661 gtcgcccggg tacccgtgta tccaataaac cctcttgcag ttgcatccga cttgtggtct
     3721 cgctgttcct tgggagggtc tcctctgagt gattgactac ccgtcagcgg gggtctttca
     3781 catgcagcat gtatcaaaat taatttggtt ttttttctta agtatttaca ttaaatggcc
     3841 atagttgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     3901 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     3961 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     4021 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     4081 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     4141 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     4201 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     4261 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     4321 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     4381 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     4441 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     4501 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     4561 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     4621 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     4681 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     4741 catgagatta tcaaaaagga tcttcaccta gatccttttg cggccgcaaa tcaatctaaa
     4801 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     4861 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     4921 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     4981 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     5041 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     5101 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     5161 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     5221 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     5281 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     5341 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     5401 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     5461 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     5521 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     5581 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     5641 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     5701 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     5761 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     5821 cagcctgaat atgggccaaa caggatatct gtggtaagca gttcctgccc cggctcaggg
     5881 ccaagaacag atggaacagc tgaatatggg ccaaacagga tatctgtggt aagcagttcc
     5941 tgccccggct cagggccaag aacagatggt ccccagatgc ggtccagccc tcagcagttt
     6001 ctagagaacc atcagatgtt tccagggtgc cccaaggacc tgaaatgacc ctgtgcctta
     6061 tttgaactaa ccaatcagtt cgcttctcgc ttctgttcgc gcgcttctgc tccccgagct
     6121 caataaaaga gcccacaacc cctcactcgg ggcgccagtc ctccgattga ctgagtcgcc
     6181 cgggtacccg tgtatccaat aaaccctctt gcagttgcat ccgacttgtg gtctcgctgt
     6241 tccttgggag ggtctcctct gagtgattga ctacccgtca gcgggggtct ttcatttggg
     6301 ggctcgtccg ggatcgggag acccctgccc agggaccacc gacccaccac cgggaggcaa
     6361 gctggccagc aacttatctg tgtctgtccg attgtctagt gtctatgact gattttatgc
     6421 gcctgcgtcg gtactagtta gctaactagc tctgtatctg gcggacccgt ggtggaactg
     6481 acgagttctg aacacccggc cgcaaccctg ggagacgtcc cagggacttt gggggccgtt
     6541 tttgtggccc gacctgagga agggagtcga tgtggaatcc gaccccgtca ggatatgtgg
     6601 ttctggtagg agacgagaac ctaaaacagt tcccgcctcc gtctgaattt ttgctttcgg
     6661 tttggaaccg aagccgcgcg tcttgtct
//
