LOCUS       sequence-271373-        6523 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Retroviral plasmid expressing GFP PSMA3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6523)
  AUTHORS   Monda JK, Cheeseman IM
  TITLE     Nde1 promotes diverse dynein functions through differential 
            interactions and exhibits an isoform-specific proteasome 
            association.
  JOURNAL   Mol Biol Cell. 2018 Sep 15;29(19):2336-2345. doi: 
            10.1091/mbc.E18-07-0418. Epub 2018 Jul 19.
  PUBMED    30024347
REFERENCE   2  (bases 1 to 6523)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E18-07-0418"; journalName: "Mol Biol Cell"; date: 
            "2018-09-15- 15"; volume: "29"; issue: "19"; pages: "2336-2345"
COMMENT     Sequence Label: pJM019
FEATURES             Location/Qualifiers
     source          1..6523
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             258..674
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     604..626
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     642..658
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             1476..1493
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1503..1526
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             1527..1550
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             join(1560..1562,1563..1565,1566..2279)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTATGITLGMDELYK"
     primer_bind     complement(1605..1626)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1866..1885)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2213..2234
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(2424..2444)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2429..2758
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2609..2744
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2766..3164
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             3333..3802
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(3932..3949)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4103..4691)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4183..4202)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4851..5642,5643..5711))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5474..5493
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5712..5816)
                     /gene="bla"
                     /label=AmpR promoter
     LTR             5847..6316
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    join(6381..6523,1..57)
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
ORIGIN
        1 gcaaccctgg gagacgtccc agggactttg ggggccgttt ttgtggcccg acctgaggaa
       61 gggagtcgat gtggaatccg accccgtcag gatatgtggt tctggtagga gacgagaacc
      121 taaaacagtt cccgcctccg tctgaatttt tgctttcggt ttggaaccga agccgcgcgt
      181 cttgtctgct gcagcgctgc agcatcgttc tgtgttgtct ctgtctgact gtgtttctgt
      241 atttgtctga aaattagggc cagactgtta ccactccctt aagtttgacc ttaggtcact
      301 ggaaagatgt cgagcggatc gctcacaacc agtcggtaga tgtcaagaag agacgttggg
      361 ttaccttctg ctctgcagaa tggccaacct ttaacgtcgg atggccgcga gacggcacct
      421 ttaaccgaga cctcatcacc caggttaaga tcaaggtctt ttcacctggc ccgcatggac
      481 acccagacca ggtcccctac atcgtgacct gggaagcctt ggcttttgac ccccctccct
      541 gggtcaagcc ctttgtacac cctaagcctc cgcctcctct tcctccatcc gccccgtctc
      601 tcccccttga acctcctcgt tcgaccccgc ctcgatcctc cctttatcca gccctcactc
      661 cttctctagg cgccggccgg atcctcgagg ccaccatgag ctcaatcggc actgggtatg
      721 acctgtcagc ctctacattc tctcctgacg gaagagtttt tcaagttgaa tatgctatga
      781 aggctgtgga aaatagtagt acagctattg gaatcagatg caaagatggt gttgtctttg
      841 gggtagaaaa attagtcctt tctaaacttt atgaagaagg ttccaacaaa agacttttta
      901 atgttgatcg gcatgttgga atggcagtag caggtttgtt ggcagatgct cgttctttag
      961 cagacatagc aagagaagaa gcttccaact tcagatctaa ctttggctac aacattccac
     1021 taaaacatct tgcagacaga gtggccatgt atgtgcatgc atatacactc tacagtgctg
     1081 ttagaccttt tggctgcagt ttcatgttag ggtcttacag tgtgaatgac ggtgcgcaac
     1141 tctacatgat tgacccatca ggtgtttcat acggttattg gggctgtgcc atcggcaaag
     1201 ccagacaagc tgcaaagacg gaaatagaga agcttcagat gaaagaaatg acctgccgtg
     1261 atatcgttaa agaagttgca aaaataattt acatagtaca tgacgaagtt aaggataaag
     1321 cttttgaact agaactcagc tgggttggtg aattaactaa tggaagacat gaaattgttc
     1381 caaaagatat aagagaagaa gcagagaaat atgctaagga atctctgaag gaagaagatg
     1441 aatcagatga tgataatatg ggccgcggca gcagccatca tcatcatcat cacagcagcg
     1501 gcctggaagt tctgttccag gggcccctgg aagttctgtt ccaggggccc tccggaggga
     1561 tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg
     1621 gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg
     1681 gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc
     1741 tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc
     1801 agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct
     1861 tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg
     1921 tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca
     1981 agctggagta caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg
     2041 gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg
     2101 accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact
     2161 acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc
     2221 tgctggagtt cgtgaccgcc accgggatca ctcttggcat ggacgagctg tacaagtaaa
     2281 gatccaccgg atctagataa ctgatcataa tcagccatac cacatttgta gaggttttac
     2341 ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattc
     2401 gccagcacag tggtcgaccc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg
     2461 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg
     2521 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     2581 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     2641 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
     2701 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
     2761 gcttcatggc caagcctttg tctcaagaag aatccaccct cattgaaaga gcaacggcta
     2821 caatcaacag catccccatc tctgaagact acagcgtcgc cagcgcagct ctctctagcg
     2881 acggccgcat cttcactggt gtcaatgtat atcattttac tgggggacct tgtgcagaac
     2941 tcgtggtgct gggcactgct gctgctgcgg cagctggcaa cctgacttgt atcgtcgcga
     3001 tcggaaatga gaacaggggc atcttgagcc cctgcggacg gtgccgacag gtgcttctcg
     3061 atctgcatcc tgggatcaaa gccatagtga aggacagtga tggacagccg acggcagttg
     3121 ggattcgtga attgctgccc tctggttatg tgtgggaggg ctaagatcga taaaataaaa
     3181 gattttattt agtctccaga aaaagggggg aatgaaagac cccacctgta ggtttggcaa
     3241 gctagcttaa gtaacgccat tttgcaaggc atggaaaaat acataactga gaatagagaa
     3301 gttcagatca aggtcaggaa cagatggaac agctgaatat gggccaaaca ggatatctgt
     3361 ggtaagcagt tcctgccccg gctcagggcc aagaacagat ggaacagctg aatatgggcc
     3421 aaacaggata tctgtggtaa gcagttcctg ccccggctca gggccaagaa cagatggtcc
     3481 ccagatgcgg tccagccctc agcagtttct agagaaccat cagatgtttc cagggtgccc
     3541 caaggacctg aaatgaccct gtgccttatt tgaactaacc aatcagttcg cttctcgctt
     3601 ctgttcgcgc gcttctgctc cccgagctca ataaaagagc ccacaacccc tcactcgggg
     3661 cgccagtcct ccgattgact gagtcgcccg ggtacccgtg tatccaataa accctcttgc
     3721 agttgcatcc gacttgtggt ctcgctgttc cttgggaggg tctcctctga gtgattgact
     3781 acccgtcagc gggggtcttt cacatgcagc atgtatcaaa attaatttgg ttttttttct
     3841 taagtattta cattaaatgg ccatagttgc attaatgaat cggccaacgc gcggggagag
     3901 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     3961 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     4021 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     4081 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     4141 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     4201 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     4261 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     4321 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     4381 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     4441 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     4501 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     4561 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     4621 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     4681 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     4741 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     4801 tgcggccgca aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     4861 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     4921 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     4981 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     5041 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     5101 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     5161 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     5221 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     5281 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     5341 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     5401 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     5461 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     5521 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     5581 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     5641 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     5701 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     5761 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     5821 tttccccgaa aagtgccacc tgcagcctga atatgggcca aacaggatat ctgtggtaag
     5881 cagttcctgc cccggctcag ggccaagaac agatggaaca gctgaatatg ggccaaacag
     5941 gatatctgtg gtaagcagtt cctgccccgg ctcagggcca agaacagatg gtccccagat
     6001 gcggtccagc cctcagcagt ttctagagaa ccatcagatg tttccagggt gccccaagga
     6061 cctgaaatga ccctgtgcct tatttgaact aaccaatcag ttcgcttctc gcttctgttc
     6121 gcgcgcttct gctccccgag ctcaataaaa gagcccacaa cccctcactc ggggcgccag
     6181 tcctccgatt gactgagtcg cccgggtacc cgtgtatcca ataaaccctc ttgcagttgc
     6241 atccgacttg tggtctcgct gttccttggg agggtctcct ctgagtgatt gactacccgt
     6301 cagcgggggt ctttcatttg ggggctcgtc cgggatcggg agacccctgc ccagggacca
     6361 ccgacccacc accgggaggc aagctggcca gcaacttatc tgtgtctgtc cgattgtcta
     6421 gtgtctatga ctgattttat gcgcctgcgt cggtactagt tagctaacta gctctgtatc
     6481 tggcggaccc gtggtggaac tgacgagttc tgaacacccg gcc
//
