LOCUS       sequence-27138-e         724 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 724)
  AUTHORS   Behm-Ansmant I, Rehwinkel J, Doerks T, Stark A, Bork P, Izaurralde E
  TITLE     mRNA degradation by miRNAs and GW182 requires both CCR4:NOT 
            deadenylase and DCP1:DCP2 decapping complexes.
  JOURNAL   Genes Dev. 2006 Jul 15. 20(14):1885-98.
  PUBMED    16815998
REFERENCE   2  (bases 1 to 724)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            2006 Jul 15. 20(14):1885-98."
COMMENT     Sequence Label: pAc5.1B-lambdaN-HA
FEATURES             Location/Qualifiers
     source          1..724
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(18..59)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             complement(132..158)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(183..248)
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
ORIGIN
        1 tgatgatgac cggtacgcgt agaatcgaga ccgaggagag ggttagggat aggcttacct
       61 tcgaaccgcg ggccctctag actcgagcgg ccgccactgt gctggatatc tgcagaattc
      121 aagcttggag gagcgtaatc tggaacatcg tatgggtaag ccatatgtgg aggagatcca
      181 ccgtttgcag ctttccattg agcttgtttc tcagcgcgac gctcacgtcg tcgtgtttgt
      241 gcgtccatgt tggtaccccg atccggggtc tctggattag acgactgctg gctgatggag
      301 cggctttgtg tcgggaggag tatccacaca gcacaagaac tcaaacggta gtgatatgaa
      361 aactggtccc gcagcccgtt ttataaagcc cgcttagcgc taaaacctta tcagccgcaa
      421 tgctctccgc tctcaagtcg cgttcaaaac ttttaccata tatgggtatg aattgttcgc
      481 cgattgggtt ttcatttacg gagaatttcc tccgcaactg ggtgttcgtg cttgtgtgtg
      541 tcttgtatat cgccatgaag agtgtacttc catgggtcct atcagttgcg gcatgtgatt
      601 caatttgggg gttactcata ttcgatactt cagctggtta tctttatgct gtgatggtca
      661 cggtgctttt acacattttt tccccaacta ctcattgtat gccaaaacat acatatgtac
      721 atgt
//
