LOCUS       sequence-271380-        6894 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Retroviral plasmid expressing GFP - Nde1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6894)
  AUTHORS   Monda JK, Cheeseman IM
  TITLE     Nde1 promotes diverse dynein functions through differential 
            interactions and exhibits an isoform-specific proteasome 
            association.
  JOURNAL   Mol Biol Cell. 2018 Sep 15;29(19):2336-2345. doi: 
            10.1091/mbc.E18-07-0418. Epub 2018 Jul 19.
  PUBMED    30024347
REFERENCE   2  (bases 1 to 6894)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E18-07-0418"; journalName: "Mol Biol Cell"; date: 
            "2018-09-15- 15"; volume: "29"; issue: "19"; pages: "2336-2345"
COMMENT     Sequence Label: pJM108
FEATURES             Location/Qualifiers
     source          1..6894
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    201..400
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             601..1017
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     947..969
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     985..1001
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     regulatory      1052..1061
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1058..1060,1061..1063,1064..1774)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1058..1060,1061..1063,1064..1774)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1103..1124)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1364..1383)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1711..1732
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             1820..1840
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1853..1897
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     primer_bind     complement(3138..3158)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3143..3472
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3323..3458
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3480..3878
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             4047..4516
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(4646..4663)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4817..5405)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4897..4916)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5565..6356,6357..6425))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6188..6207
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6426..6530)
                     /gene="bla"
                     /label=AmpR promoter
     LTR             join(6561..6894,1..136)
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
ORIGIN
        1 tcctccgatt gactgagtcg cccgggtacc cgtgtatcca ataaaccctc ttgcagttgc
       61 atccgacttg tggtctcgct gttccttggg agggtctcct ctgagtgatt gactacccgt
      121 cagcgggggt ctttcatttg ggggctcgtc cgggatcggg agacccctgc ccagggacca
      181 ccgacccacc accgggaggc aagctggcca gcaacttatc tgtgtctgtc cgattgtcta
      241 gtgtctatga ctgattttat gcgcctgcgt cggtactagt tagctaacta gctctgtatc
      301 tggcggaccc gtggtggaac tgacgagttc tgaacacccg gccgcaaccc tgggagacgt
      361 cccagggact ttgggggccg tttttgtggc ccgacctgag gaagggagtc gatgtggaat
      421 ccgaccccgt caggatatgt ggttctggta ggagacgaga acctaaaaca gttcccgcct
      481 ccgtctgaat ttttgctttc ggtttggaac cgaagccgcg cgtcttgtct gctgcagcgc
      541 tgcagcatcg ttctgtgttg tctctgtctg actgtgtttc tgtatttgtc tgaaaattag
      601 ggccagactg ttaccactcc cttaagtttg accttaggtc actggaaaga tgtcgagcgg
      661 atcgctcaca accagtcggt agatgtcaag aagagacgtt gggttacctt ctgctctgca
      721 gaatggccaa cctttaacgt cggatggccg cgagacggca cctttaaccg agacctcatc
      781 acccaggtta agatcaaggt cttttcacct ggcccgcatg gacacccaga ccaggtcccc
      841 tacatcgtga cctgggaagc cttggctttt gacccccctc cctgggtcaa gccctttgta
      901 caccctaagc ctccgcctcc tcttcctcca tccgccccgt ctctccccct tgaacctcct
      961 cgttcgaccc cgcctcgatc ctccctttat ccagccctca ctccttctct aggcgccggc
     1021 cggatcccag tgtggtggta cgctaccggt cgccaccatg gtgagcaagg gcgaggagct
     1081 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
     1141 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat
     1201 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg
     1261 cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc
     1321 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
     1381 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
     1441 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag
     1501 ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat
     1561 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc
     1621 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct
     1681 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
     1741 cgggatcact ctcggcatgg acgagctgta caagtccgga ggggatcggt ggagctccac
     1801 cggtggcggc cgctctagag agaatcttta ttttcagggc gccgccaaat tcaaagaaac
     1861 cgctgctgct aaattcgaac gccagcacat ggacagcgga ggtggaggta ctagtctcga
     1921 gatggaagat agcggcaaaa ccttcagcag cgaagaagaa gaggcgaatt actggaagga
     1981 cctggccatg acctacaagc agcgtgccga aaatacccag gaagaactgc gcgaatttca
     2041 agaggggagc cgcgaatatg aggccgagct ggaaacacag ctgcagcaga tcgagacacg
     2101 caatcgcgat ctgctgagcg aaaataaccg cctgcgcatg gaactggaaa caattaaaga
     2161 gaaattcgag gtgcagcata gcgagggcta tcgccaaatt agcgccctgg aggatgatct
     2221 ggcccagacc aaagccatca aagatcagct gcagaaatac attcgcgagc tggagcaggc
     2281 aaatgatgat ctggaacgcg ccaaacgcgc caccattatg agtctggagg actttgagca
     2341 gcgtctgaac caggcaattg agcgcaatgc cttcctggaa agcgaattag atgagaaaga
     2401 gaatctgtta gagagtgtgc agcgcctgaa agacgaagcc cgtgacctgc gccaggagct
     2461 ggcagtgcag cagaagcaag aaaagccgcg caccccgatg ccgagtagtg ttgaagcaga
     2521 acgtaccgat accgcagtgc aagccaccgg cagcgttccg agtacaccga ttgcccatcg
     2581 cggcccgagt agcagtctga ataccccggg cagctttcgt cgtggtctgg atgatagcac
     2641 cggtggcaca ccgttaacac cggccgcccg tattagcgcc ctgaatattg tgggcgatct
     2701 gctgcgcaaa gtgggcgcac tggagagtaa actggccagt tgccgcaacc tggtgtatga
     2761 tcagagcccg aatcgtacag gtggtccggc aagcggccgc agtagtaaaa atcgtgatgg
     2821 cggcgaacgc cgtccgagta gtaccagcgt tccgctgggc gacaaaggcc tgggcaagcg
     2881 gctggagttc ggcaagcccc ctagccacat gagctcctct cccctgccca gcgcccaggg
     2941 cgtggtcaag atgctgctct gagaattctg cagtcgacgg taccgcgggc ccgggatcca
     3001 ccggatctag ataactgatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt
     3061 taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca attcgccagc
     3121 acagtggtcg accctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
     3181 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
     3241 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
     3301 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     3361 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     3421 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagcttca
     3481 tggccaagcc tttgtctcaa gaagaatcca ccctcattga aagagcaacg gctacaatca
     3541 acagcatccc catctctgaa gactacagcg tcgccagcgc agctctctct agcgacggcc
     3601 gcatcttcac tggtgtcaat gtatatcatt ttactggggg accttgtgca gaactcgtgg
     3661 tgctgggcac tgctgctgct gcggcagctg gcaacctgac ttgtatcgtc gcgatcggaa
     3721 atgagaacag gggcatcttg agcccctgcg gacggtgccg acaggtgctt ctcgatctgc
     3781 atcctgggat caaagccata gtgaaggaca gtgatggaca gccgacggca gttgggattc
     3841 gtgaattgct gccctctggt tatgtgtggg agggctaaga tcgataaaat aaaagatttt
     3901 atttagtctc cagaaaaagg ggggaatgaa agaccccacc tgtaggtttg gcaagctagc
     3961 ttaagtaacg ccattttgca aggcatggaa aaatacataa ctgagaatag agaagttcag
     4021 atcaaggtca ggaacagatg gaacagctga atatgggcca aacaggatat ctgtggtaag
     4081 cagttcctgc cccggctcag ggccaagaac agatggaaca gctgaatatg ggccaaacag
     4141 gatatctgtg gtaagcagtt cctgccccgg ctcagggcca agaacagatg gtccccagat
     4201 gcggtccagc cctcagcagt ttctagagaa ccatcagatg tttccagggt gccccaagga
     4261 cctgaaatga ccctgtgcct tatttgaact aaccaatcag ttcgcttctc gcttctgttc
     4321 gcgcgcttct gctccccgag ctcaataaaa gagcccacaa cccctcactc ggggcgccag
     4381 tcctccgatt gactgagtcg cccgggtacc cgtgtatcca ataaaccctc ttgcagttgc
     4441 atccgacttg tggtctcgct gttccttggg agggtctcct ctgagtgatt gactacccgt
     4501 cagcgggggt ctttcacatg cagcatgtat caaaattaat ttggtttttt ttcttaagta
     4561 tttacattaa atggccatag ttgcattaat gaatcggcca acgcgcgggg agaggcggtt
     4621 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     4681 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     4741 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     4801 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     4861 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     4921 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     4981 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     5041 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     5101 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     5161 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     5221 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     5281 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     5341 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     5401 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     5461 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttgcggc
     5521 cgcaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     5581 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     5641 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     5701 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     5761 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     5821 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     5881 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     5941 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     6001 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     6061 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     6121 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     6181 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     6241 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     6301 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     6361 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     6421 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     6481 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     6541 cgaaaagtgc cacctgcagc ctgaatatgg gccaaacagg atatctgtgg taagcagttc
     6601 ctgccccggc tcagggccaa gaacagatgg aacagctgaa tatgggccaa acaggatatc
     6661 tgtggtaagc agttcctgcc ccggctcagg gccaagaaca gatggtcccc agatgcggtc
     6721 cagccctcag cagtttctag agaaccatca gatgtttcca gggtgcccca aggacctgaa
     6781 atgaccctgt gccttatttg aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc
     6841 ttctgctccc cgagctcaat aaaagagccc acaacccctc actcggggcg ccag
//
