LOCUS       sequence-271381-         701 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR-Cas9 library validation (positive control).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 701)
  AUTHORS   Arnan C, Ullrich S, Pulido-Quetglas C, Nurtdinov R, Esteban A, 
            Blanco-Fernandez J, Aparicio-Prat E, Johnson R, Perez-Lluch S, Guigo
            R
  TITLE     Paired guide RNA CRISPR-Cas9 screening for protein-coding genes and 
            lncRNAs involved in transdifferentiation of human B-cells to 
            macrophages.
  JOURNAL   BMC Genomics. 2022 May 26;23(1):402. doi: 
            10.1186/s12864-022-08612-7.
  PUBMED    35619054
REFERENCE   2  (bases 1 to 701)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Genomics."; date: "2022-05-26"; volume: "23(1):402. doi"; pages: " 
            10.1186/s12864-022-08612-7"
COMMENT     Sequence Label: pDECKO_mCherry_Rat_cebp-alfa
FEATURES             Location/Qualifiers
     source          1..701
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     43..62
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        143..218
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        225..439
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     360..379
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     misc_RNA        468..543
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 aattnttggn tagtttgcag ttttaaatta tgttttaaaa tggactatca tatgcttacc
       61 gtaacttgaa agtatttcga tttcttggct ttatatatct tgtggaaagg acgaaacacc
      121 ggtgctcgca gatgccgccc aggttttaga gctagaaata gcaagttaaa ataaggctag
      181 tccgttatca acttgaaaaa gtggcaccga gtcggtgctt ttttgaacgc tgacgtcatc
      241 aacccgctcc aaggaatcgc gggcccagtg tcactaggcg ggaacaccca gcgcgcgtgc
      301 gccctggcag gaagatggct gtgagggaca ggggagtggc gccctgcaat atttgcatgt
      361 cgctatgtgt tctgggaaat caccataaac gtgaaatgtc tttggatttg ggagtcttat
      421 aagttctgta tgagaccact ctttccccag gaactcgtcg ttgaagggtt ttagagctag
      481 aaatagcaag ttaaaataag gctagtccgt tatcaacttg aaaaagtggc accgagtcgg
      541 tgctttttta agcttggcgt aactagatct tgagacaaat ggcagtattc atccacaatt
      601 ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag
      661 caacagacat acaaactaaa gaattacaaa aacaaattac a
//
