LOCUS       sequence-271383-        5977 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Retroviral plasmid expressing GFP - Nde1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5977)
  AUTHORS   Monda JK, Cheeseman IM
  TITLE     Nde1 promotes diverse dynein functions through differential 
            interactions and exhibits an isoform-specific proteasome 
            association.
  JOURNAL   Mol Biol Cell. 2018 Sep 15;29(19):2336-2345. doi: 
            10.1091/mbc.E18-07-0418. Epub 2018 Jul 19.
  PUBMED    30024347
REFERENCE   2  (bases 1 to 5977)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E18-07-0418"; journalName: "Mol Biol Cell"; date: 
            "2018-09-15- 15"; volume: "29"; issue: "19"; pages: "2336-2345"
COMMENT     Sequence Label: pJM110
FEATURES             Location/Qualifiers
     source          1..5977
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             121..537
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     467..489
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     505..521
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     regulatory      572..581
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(578..580,581..583,584..1294)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(578..580,581..583,584..1294)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(623..644)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(884..903)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1231..1252
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             1340..1360
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1373..1417
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     primer_bind     complement(1741..1761)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        1746..2075
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      1926..2061
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2083..2481
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             2650..3119
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(3249..3266)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3420..4008)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3500..3519)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4168..4959,4960..5028))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4791..4810
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5029..5133)
                     /gene="bla"
                     /label=AmpR promoter
     LTR             5164..5633
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    5698..5897
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
ORIGIN
        1 ccgtctgaat ttttgctttc ggtttggaac cgaagccgcg cgtcttgtct gctgcagcgc
       61 tgcagcatcg ttctgtgttg tctctgtctg actgtgtttc tgtatttgtc tgaaaattag
      121 ggccagactg ttaccactcc cttaagtttg accttaggtc actggaaaga tgtcgagcgg
      181 atcgctcaca accagtcggt agatgtcaag aagagacgtt gggttacctt ctgctctgca
      241 gaatggccaa cctttaacgt cggatggccg cgagacggca cctttaaccg agacctcatc
      301 acccaggtta agatcaaggt cttttcacct ggcccgcatg gacacccaga ccaggtcccc
      361 tacatcgtga cctgggaagc cttggctttt gacccccctc cctgggtcaa gccctttgta
      421 caccctaagc ctccgcctcc tcttcctcca tccgccccgt ctctccccct tgaacctcct
      481 cgttcgaccc cgcctcgatc ctccctttat ccagccctca ctccttctct aggcgccggc
      541 cggatcccag tgtggtggta cgctaccggt cgccaccatg gtgagcaagg gcgaggagct
      601 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
      661 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat
      721 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg
      781 cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc
      841 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
      901 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
      961 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag
     1021 ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat
     1081 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc
     1141 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct
     1201 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
     1261 cgggatcact ctcggcatgg acgagctgta caagtccgga ggggatcggt ggagctccac
     1321 cggtggcggc cgctctagag agaatcttta ttttcagggc gccgccaaat tcaaagaaac
     1381 cgctgctgct aaattcgaac gccagcacat ggacagcgga ggtggaggta ctagtctcga
     1441 gatggcctct gggcggagca gcaagaacag agatggcggg gagagacggc caagcagcac
     1501 cagcgtgcct ttgggtgata aggggttgga cacgagttgc cgctggttgt ccaaatcaac
     1561 aaccaggtcg tccagctcct gctgaccgcg ggcccgggat ccaccggatc tagataactg
     1621 atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac
     1681 ctccccctga acctgaaaca taaaatgaat gcaattcgcc agcacagtgg tcgaccctgt
     1741 ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     1801 aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag
     1861 gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc
     1921 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     1981 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
     2041 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct tcatggccaa gcctttgtct
     2101 caagaagaat ccaccctcat tgaaagagca acggctacaa tcaacagcat ccccatctct
     2161 gaagactaca gcgtcgccag cgcagctctc tctagcgacg gccgcatctt cactggtgtc
     2221 aatgtatatc attttactgg gggaccttgt gcagaactcg tggtgctggg cactgctgct
     2281 gctgcggcag ctggcaacct gacttgtatc gtcgcgatcg gaaatgagaa caggggcatc
     2341 ttgagcccct gcggacggtg ccgacaggtg cttctcgatc tgcatcctgg gatcaaagcc
     2401 atagtgaagg acagtgatgg acagccgacg gcagttggga ttcgtgaatt gctgccctct
     2461 ggttatgtgt gggagggcta agatcgataa aataaaagat tttatttagt ctccagaaaa
     2521 aggggggaat gaaagacccc acctgtaggt ttggcaagct agcttaagta acgccatttt
     2581 gcaaggcatg gaaaaataca taactgagaa tagagaagtt cagatcaagg tcaggaacag
     2641 atggaacagc tgaatatggg ccaaacagga tatctgtggt aagcagttcc tgccccggct
     2701 cagggccaag aacagatgga acagctgaat atgggccaaa caggatatct gtggtaagca
     2761 gttcctgccc cggctcaggg ccaagaacag atggtcccca gatgcggtcc agccctcagc
     2821 agtttctaga gaaccatcag atgtttccag ggtgccccaa ggacctgaaa tgaccctgtg
     2881 ccttatttga actaaccaat cagttcgctt ctcgcttctg ttcgcgcgct tctgctcccc
     2941 gagctcaata aaagagccca caacccctca ctcggggcgc cagtcctccg attgactgag
     3001 tcgcccgggt acccgtgtat ccaataaacc ctcttgcagt tgcatccgac ttgtggtctc
     3061 gctgttcctt gggagggtct cctctgagtg attgactacc cgtcagcggg ggtctttcac
     3121 atgcagcatg tatcaaaatt aatttggttt tttttcttaa gtatttacat taaatggcca
     3181 tagttgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     3241 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     3301 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     3361 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     3421 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     3481 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     3541 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     3601 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     3661 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     3721 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     3781 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     3841 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     3901 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     3961 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     4021 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     4081 atgagattat caaaaaggat cttcacctag atccttttgc ggccgcaaat caatctaaag
     4141 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     4201 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     4261 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     4321 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     4381 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     4441 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     4501 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     4561 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     4621 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     4681 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     4741 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     4801 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     4861 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     4921 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     4981 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     5041 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     5101 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctgc
     5161 agcctgaata tgggccaaac aggatatctg tggtaagcag ttcctgcccc ggctcagggc
     5221 caagaacaga tggaacagct gaatatgggc caaacaggat atctgtggta agcagttcct
     5281 gccccggctc agggccaaga acagatggtc cccagatgcg gtccagccct cagcagtttc
     5341 tagagaacca tcagatgttt ccagggtgcc ccaaggacct gaaatgaccc tgtgccttat
     5401 ttgaactaac caatcagttc gcttctcgct tctgttcgcg cgcttctgct ccccgagctc
     5461 aataaaagag cccacaaccc ctcactcggg gcgccagtcc tccgattgac tgagtcgccc
     5521 gggtacccgt gtatccaata aaccctcttg cagttgcatc cgacttgtgg tctcgctgtt
     5581 ccttgggagg gtctcctctg agtgattgac tacccgtcag cgggggtctt tcatttgggg
     5641 gctcgtccgg gatcgggaga cccctgccca gggaccaccg acccaccacc gggaggcaag
     5701 ctggccagca acttatctgt gtctgtccga ttgtctagtg tctatgactg attttatgcg
     5761 cctgcgtcgg tactagttag ctaactagct ctgtatctgg cggacccgtg gtggaactga
     5821 cgagttctga acacccggcc gcaaccctgg gagacgtccc agggactttg ggggccgttt
     5881 ttgtggcccg acctgaggaa gggagtcgat gtggaatccg accccgtcag gatatgtggt
     5941 tctggtagga gacgagaacc taaaacagtt cccgcct
//
