LOCUS       sequence-271395-       10082 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses CPT1A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10082)
  AUTHORS   Galligan JT, Martinez-Noel G, Arndt V, Hayes S, Chittenden TW, 
            Harper JW, Howley PM
  TITLE     Proteomic analysis and identification of cellular interactors of the
            giant ubiquitin ligase HERC2.
  JOURNAL   J Proteome Res. 2015 Feb 6;14(2):953-66. doi: 10.1021/pr501005v. 
            Epub 2014 Dec 15.
  PUBMED    25476789
REFERENCE   2  (bases 1 to 10082)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/pr501005v";
            journalName: "J Proteome Res"; date: "2015-02-6- 6"; volume: "14"; 
            issue: "2"; pages: "953-66"
COMMENT     Sequence Label: p7056 pHAGE-P-CMVt-N-HA-GAW-CPT1A
FEATURES             Location/Qualifiers
     source          1..10082
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    52..169
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        221..524
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        525..728
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     679..699
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    730..748
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    751..769
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     779..803
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      890..899
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             902..928
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    944..968
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3237..3261)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        3280..3779
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3315..3338)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     3712..3731
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             3800..4399
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3800..3819)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4296..4316
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    4466..5054
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4519..4539)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4937..4948)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5129..5362
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(5481..5499)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5567..5671
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5672..5740,5741..6532)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5890..5909)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6703..7291
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7192..7211
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7445..7462
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7702..7723
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7738..7743,7744..7761,7762..7768)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7776..7792
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7781..7803
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7800..7816
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7800..7816
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7866..7885
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             8077..8710
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    8757..8882
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9379..9612
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9797..9841
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 acggatctcg acggtatcgc cgaattcaca aatggcagta ttcatccaca attttaaaag
       61 aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga
      121 catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaattttc gggtttatta
      181 cagggacagc agagatccag tttggactag tggagttccg cgttacataa cttacggtaa
      241 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
      301 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
      361 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
      421 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
      481 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
      541 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
      601 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
      661 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
      721 gcagagctct ccctatcagt gatagagatc tccctatcag tgatagagat cgtcgacgag
      781 ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata
      841 gaagacaccg ggaccgatcc agcctccgga ctctagcgtt taaacgctag ccaccatggc
      901 gtacccatac gatgttccag attacgctgc tcgaggttat caaacaagtt tgtacaaaaa
      961 agttggcatg gcagaagctc accaagctgt ggcctttcag ttcacggtca ctccggacgg
     1021 gattgacctg cggctgagcc atgaagctct tagacaaatc tatctctctg gacttcattc
     1081 ctggaaaaag aagttcatca gattcaagaa cggcatcatc actggcgtgt acccggcaag
     1141 cccctccagt tggcttatcg tggtggtggg cgtgatgaca acgatgtacg ccaagatcga
     1201 cccctcgtta ggaataattg caaaaatcaa tcggactctg gaaacggcca actgcatgtc
     1261 cagccagacg aagaacgtgg tcagcggcgt gctgtttggc accggcctgt gggtggccct
     1321 catcgtcacc atgcgctact ccctgaaagt gctgctctcc taccacgggt ggatgttcac
     1381 tgagcacggc aagatgagtc gtgccaccaa gatctggatg ggtatggtca agatcttttc
     1441 aggccgaaaa cccatgttgt acagcttcca gacatcgctg cctcgcctgc cggtcccggc
     1501 tgtcaaagac actgtgaaca ggtatctaca gtcggtgagg cctcttatga aggaagaaga
     1561 cttcaaacgg atgacagcac ttgctcaaga ttttgctgtc ggtcttggac caagattaca
     1621 gtggtatttg aagttaaaat cctggtgggc tacaaattac gtgagcgact ggtgggagga
     1681 gtacatctac ctccgaggac gagggccgct catggtgaac agcaactatt atgccatgga
     1741 tctgctgtat atccttccaa ctcacattca ggcagcaaga gccggcaacg ccatccatgc
     1801 catcctgctt tacaggcgca aactggaccg ggaggaaatc aaaccaattc gtcttttggg
     1861 atccacgatt ccactctgct ccgctcagtg ggagcggatg tttaatactt cccggatccc
     1921 aggagaggag acagacacca tccagcacat gagagacagc aagcacatcg tcgtgtacca
     1981 tcgaggacgc tacttcaagg tctggctcta ccatgatggg cggctgctga agccccggga
     2041 gatggagcag cagatgcaga ggatcctgga caatacctcg gagcctcagc ccggggaggc
     2101 caggctggca gccctcaccg caggagacag agttccctgg gccaggtgtc gtcaggccta
     2161 ttttggacgt gggaaaaata agcagtctct tgatgctgtg gagaaagcag cgttcttcgt
     2221 gacgttagat gaaactgaag aaggatacag aagtgaagac ccggatacgt caatggacag
     2281 ctacgccaaa tctctactac acggccgatg ttacgacagg tggtttgaca agtcgttcac
     2341 gtttgttgtc ttcaaaaacg ggaagatggg cctcaacgct gaacactcct gggcagatgc
     2401 gccgatcgtg gcccaccttt gggagtacgt catgtccatt gacagcctcc agctgggcta
     2461 tgcggaggat gggcactgca aaggcgacat caatccgaac attccgtacc ccaccaggct
     2521 gcagtgggac atcccggggg aatgtcaaga ggttatagag acctccctga acaccgcaaa
     2581 tcttctggca aacgacgtgg atttccattc cttcccattc gtagcctttg gtaaaggaat
     2641 catcaagaaa tgtcgcacga gcccagacgc ctttgtgcag ctggccctcc agctggcgca
     2701 ctacaaggac atgggcaagt tttgcctcac atacgaggcc tccatgaccc ggctcttccg
     2761 agaggggagg acggagaccg tgcgctcctg caccactgag tcatgcgact tcgtgcgggc
     2821 catggtggac ccggcccaga cggtggaaca gaggctgaag ttgttcaagt tggcgtctga
     2881 gaagcatcag catatgtatc gcctcgccat gaccggctct gggatcgatc gtcacctctt
     2941 ctgcctttac gtggtgtcta aatatctcgc tgtggagtcc cctttcctta aggaagtttt
     3001 atctgagcct tggagattat caacaagcca gacccctcag cagcaagtgg agctgtttga
     3061 cttggagaat aacccagagt acgtgtccag cggagggggc tttggaccgg ttgctgatga
     3121 cggctatggt gtgtcgtaca tccttgtggg agagaacctc atcaatttcc acatttcttc
     3181 caagttctct tgccctgaga cggggattat aagtcaagga ccaagttcag atacttgccc
     3241 aactttcttg tacaaagtgg ttcgataacg aattctaccg ggtaggggag gcgcttttcc
     3301 caaggcagtc tggagcatgc gctttagcag ccccgctggg cacttggcgc tacacaagtg
     3361 gcctctggcc tcgcacacat tccacatcca ccggtaggcg ccaaccggct ccgttctttg
     3421 gtggcccctt cgcgccacct tctactcctc ccctagtcag gaagttcccc cccgccccgc
     3481 agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg tctcactagt ctcgtgcaga
     3541 tggacagcac cgctgagcaa tggaagcggg taggcctttg gggcagcggc caatagcagc
     3601 tttgctcctt cgctttctgg gctcagaggc tgggaagggg tgggtccggg ggcgggctca
     3661 ggggngggct caggggcggg gcgggcgccc gaaggtcctc cggaggcccg gcattctgca
     3721 cgcttcaaaa gcgcacgtct gccgcgctgt tctcctcttc ctcatctccg ggcctttcga
     3781 cctgcagccc aagcttacca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga
     3841 cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg
     3901 ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct
     3961 cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc
     4021 ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg
     4081 catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc
     4141 gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca
     4201 ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc
     4261 cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct
     4321 cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac
     4381 ccgcaagccc ggtgcctgac gcccgcccca cgacccgcag cgcccgaccg aaaggagcgc
     4441 acgaccccat gcatcgatag atcctaatca acctctggat tacaaaattt gtgaaagatt
     4501 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
     4561 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
     4621 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
     4681 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
     4741 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
     4801 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
     4861 atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc
     4921 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
     4981 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg
     5041 ggccgcctcc ccgcctgaga tcctttaaga ccaatgactt acaaggcagc tgtagatctt
     5101 agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa
     5161 gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
     5221 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     5281 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     5341 tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa
     5401 cttgcaaaga aatgaatatc agagagtgag aggcccgggt taattaagga aagggctaga
     5461 tcattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
     5521 aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     5581 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     5641 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     5701 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     5761 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     5821 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     5881 taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg
     5941 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     6001 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     6061 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     6121 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     6181 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
     6241 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     6301 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     6361 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     6421 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     6481 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     6541 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     6601 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     6661 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     6721 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     6781 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     6841 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     6901 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     6961 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     7021 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     7081 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     7141 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     7201 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     7261 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     7321 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     7381 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     7441 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc
     7501 cgcgcgttgg ccgattcatt aatgcagcaa gctcatggct gactaatttt ttttatttat
     7561 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
     7621 ggaggcctag gcttttgcaa aaagctcccc gtggcacgac aggtttcccg actggaaagc
     7681 gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt
     7741 acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac
     7801 aggaaacagc tatgacatga ttacgaattt cacaaataaa gcattttttt cactgcattc
     7861 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatca actggataac
     7921 tcaagctaac caaaatcatc ccaaacttcc caccccatac cctattacca ctgccaatta
     7981 cctgtggttt catttactct aaacctgtga ttcctctgaa ttattttcat tttaaagaaa
     8041 ttgtatttgt taaatatgta ctacaaactt agtagttgga agggctaatt cactcccaaa
     8101 gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc cctgattagc
     8161 agaactacac accagggcca ggggtcagat atccactgac ctttggatgg tgctacaagc
     8221 tagtaccagt tgagccagat aaggtagaag aggccaataa aggagagaac accagcttgt
     8281 tacaccctgt gagcctgcat gggatggatg acccggagag agaagtgtta gagtggaggt
     8341 ttgacagccg cctagcattt catcacgtgg cccgagagct gcatccggag tacttcaaga
     8401 actgctgata tcgagcttgc tacaagggac tttccgctgg ggactttcca gggaggcgtg
     8461 gcctgggcgg gactggggag tggcgagccc tcagatcctg catataagca gctgcttttt
     8521 gcctgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     8581 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     8641 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     8701 atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct
     8761 ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg
     8821 tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg
     8881 tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg
     8941 gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg
     9001 cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac
     9061 aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc
     9121 tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag
     9181 aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccggccgc tgatcttcag
     9241 acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt
     9301 aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga
     9361 aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac
     9421 tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt
     9481 gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac
     9541 agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga
     9601 tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc
     9661 ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat
     9721 ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc
     9781 gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt
     9841 gtggaattgg tttaacataa caaattggct gtggtatata aaattattca taatgatagt
     9901 aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag
     9961 gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag
    10021 gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt
    10081 ga
//
