LOCUS       sequence-271404-        9381 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentivirus for base editing activatable GFP expression in mammalian 
            cells. All-in-one vector with sgGO. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9381)
  AUTHORS   Katti A, Foronda M, Zimmerman J, Diaz B, Zafra MP, Goswami S, Dow LE
  TITLE     GO: a functional reporter system to identify and enrich base editing
            activity.
  JOURNAL   Nucleic Acids Res. 2020 Feb 29. pii: 5766653. doi: 
            10.1093/nar/gkaa124.
  PUBMED    32112097
REFERENCE   2  (bases 1 to 9381)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Feb 29. pii: 5766653. doi: 10.1093/nar/gkaa124."
COMMENT     Sequence Label: pRRL-mU6-GFPGO2-IRES-mScarletI
FEATURES             Location/Qualifiers
     source          1..9381
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1806..1923
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1982..2295
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for mouse U6 snRNA (Das 
                     et al., 1988)"
     primer_bind     2214..2234
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     CDS             2870..2890
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2900..2920
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(2918..2920,2921..3634)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(2918..2920,2921..3631)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(2960..2981)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3221..3240)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3568..3589
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    3722..4274
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3866..3883)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4093..4112
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4298..4318
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4322..4342
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4346..4366
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     4980..5003
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     misc_feature    5109..5697
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5162..5182)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5580..5591)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5904..6137
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6209..6330
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6246..6265)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6300..6319
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6370..6505
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6525..6544)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6526..6544)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6554..6571)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6554..6570)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6563..6585)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6712..7167
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6799..6818)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7009..7030
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7193..7297
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7298..7366,7367..8158)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7516..7535)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8329..8917
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8818..8837
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9071..9088
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9205..9226
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9241..9246,9247..9264,9265..9271)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9279..9295
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9284..9306
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9303..9319
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9303..9319
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9338..9358
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9340..9358
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
     1741 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcgg
     1801 ttaactttta aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac
     1861 ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat
     1921 tttatcgatc acgagactag cctcgatcgc aattctgcag acaaatggct ctagatctag
     1981 agatccgacg ccgccatctc taggcccgcg ccggccccct cgcacagact tgtgggagaa
     2041 gctcggctac tcccctgccc cggttaattt gcatataata tttcctagta actatagagg
     2101 cttaatgtgc gataaaagac agataatctg ttctttttaa tactagctac attttacatg
     2161 ataggcttgg atttctataa gagatacaaa tactaaatta ttattttaaa aaacagcaca
     2221 aaaggaaact caccctaact gtaaagtaat tgtgtgtttt gagactataa atatgcatgc
     2281 gagaaaagcc ttgtttgcac gacggtaagc aagggcgttt aagagctatg ctggaaacag
     2341 catagcaagt ttaaataagg ctagtccgtt atcaacttga aaaagtggca ccgagtcggt
     2401 gcttttttgt tttaagtctc gagatcgagg gctagcgact agctgcagta acgccatttt
     2461 gcaaggcatg gaaaaatacc aaaccaagaa tagagaagtt cagatcaagg gcgggtacat
     2521 gaaaatagct aacgttgggc caaacaggat atctgcggtg agcagtttcg gccccggccc
     2581 ggggccaaga acagatggtc accgcagttt cggccccggc ccgaggccaa gaacagatgg
     2641 tccccagata tggcccaacc ctcagcagtt tcttaagacc catcagatgt ttccaggctc
     2701 ccccaaggac ctgaaatgac cctgcgcctt atttgaatta accaatcagc ctgcttctcg
     2761 cttctgttcg cgcgcttctg cttcccgagc tctataaaag agctcacaac ccctcactcg
     2821 gcgcgccagt cctccgacag aattcagcac gacggtaagc aagggcgggc caaagaagaa
     2881 acggaaagta ggcggctccc ccaagaagaa gcggaaggta agcaagggcg aggagctgtt
     2941 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
     3001 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg
     3061 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt
     3121 gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
     3181 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac
     3241 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
     3301 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca
     3361 caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg
     3421 ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat
     3481 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag
     3541 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg
     3601 gatcactctc ggcatggacg agctgtacaa gtaatgacgt ctccccgggt aatccggact
     3661 cgacctgcag ccaagcttat cacgcgttga gaacttcagg gtgagtttgg gcccccccct
     3721 aacgttactg gccgaagccg cttggaataa ggccggtgtg cgtttgtcta tatgttattt
     3781 tccaccatat tgccgtcttt tggcaatgtg agggcccgga aacctggccc tgtcttcttg
     3841 acgagcattc ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc
     3901 gtgaaggaag cagttcctct ggaagcttct tgaagacaaa caacgtctgt agcgaccctt
     3961 tgcaggcagc ggaacccccc acctggcgac aggtgcctct gcggccaaaa gccacgtgta
     4021 taagatacac ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg
     4081 gaaagagtca aatggctctc ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag
     4141 gtaccccatt gtatgggatc tgatctgggg cctcggtgca catgctttac atgtgtttag
     4201 tcgaggttaa aaaaacgtct aggccccccg aaccacgggg acgtggtttt cctttgaaaa
     4261 acacgatgat aatatggcca caaccatggg cagcgaccca aagaagaagc ggaaagtgga
     4321 ccccaagaaa aagagaaagg tggaccccaa aaagaagcgg aaggtgggca gccccgtggc
     4381 cacaatggtg agcaagggcg aggccgtgat caaggagttc atgaggttca aggtgcacat
     4441 ggagggctcc atgaacggtc acgagttcga aatcgagggc gaaggcgagg gcaggcccta
     4501 cgagggcacc cagaccgcca agctgaaggt gacaaagggc ggccccctgc ccttctcctg
     4561 ggacatcctg tcccctcagt tcatgtacgg ctccagggcc ttcatcaagc accccgccga
     4621 catccccgac tactacaagc agtccttccc cgagggcttc aagtgggaga gggtgatgaa
     4681 cttcgaggac ggcggcgccg tgaccgtgac ccaggacacc tccctggagg acggcaccct
     4741 gatctacaag gtgaagctga ggggcaccaa cttccctcct gacggccccg tgatgcagaa
     4801 gaagacaatg ggctgggagg cctccaccga gaggctgtac cccgaggacg gcgtgctgaa
     4861 gggcgacatc aagatggccc tgaggctgaa ggacggcggc agatacctgg ccgacttcaa
     4921 gaccacctac aaggccaaga agcccgtgca gatgcccggc gcctacaacg tggacaggaa
     4981 gctggacatc acctcccaca acgaggacta caccgtggtg gagcagtacg agaggtccga
     5041 gggcaggcac tccaccggcg gcatggacga gctgtataag tgagtcgact tcgaaacgcg
     5101 tctggaacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
     5161 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
     5221 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
     5281 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
     5341 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
     5401 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
     5461 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg tcctttccat
     5521 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
     5581 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc
     5641 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg
     5701 gaattaattc tgcagtcgag acctagaaaa acatggagca atcacaagta gcaatacagc
     5761 agctaccaat gctgattgtg cctggctaga agcacaagag gaggaggagg tgggttttcc
     5821 agtcacacct caggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca
     5881 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct
     5941 gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     6001 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     6061 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     6121 gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc
     6181 aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa
     6241 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     6301 tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta
     6361 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     6421 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
     6481 tagtgaggag gcttttttgg aggcctaggg acgtacccaa ttcgccctat agtgagtcgt
     6541 attacgcgcg ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta
     6601 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg
     6661 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg gacgcgccct
     6721 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     6781 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     6841 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
     6901 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
     6961 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     7021 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
     7081 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     7141 ttaacaaaat attaacgctt acaatttagg tggcactttt cggggaaatg tgcgcggaac
     7201 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     7261 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     7321 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     7381 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     7441 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     7501 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
     7561 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     7621 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     7681 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     7741 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     7801 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     7861 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     7921 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     7981 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     8041 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     8101 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     8161 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     8221 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     8281 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     8341 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     8401 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     8461 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     8521 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     8581 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     8641 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     8701 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     8761 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     8821 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     8881 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     8941 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     9001 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     9061 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc
     9121 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
     9181 agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc
     9241 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca
     9301 cacaggaaac agctatgacc atgattacgc caagcgcgca attaaccctc actaaaggga
     9361 acaaaagctg gagctgcaag c
//
