LOCUS       sequence-271406-       10704 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses fluorescently-tagged human Type I procollagen alpha-1 
            chain with mCerulean replacing exon 2-3 retaining N-propeptide minor
            triple helix and N-terminal cleavage site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10704)
  TITLE     Fluorescent protein-tagged human type I procollagen expression 
            vectors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10704)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: mCerulean-hCOL1A1
FEATURES             Location/Qualifiers
     source          1..10704
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     844..863
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        844..862
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(871..904)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             join(1141..1143,1144..1851)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIKA
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELY"
     primer_bind     complement(1183..1204)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1444..1463)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     complement(6252..6269)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6258..6482
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6528..6956
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6615..6634)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6825..6846
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6965..6985)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6970..7299
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7150..7285
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7212..7231
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             7366..8160
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7420..7439)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8030..8049
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8334..8455
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8371..8390)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8425..8444
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8504..8520)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8504..8520)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8517..8539)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8528..8544
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8552..8558,8559..8576,8577..8582))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8597..8618
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8735..8752)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8906..9491)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8986..9005)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9662..10453,10454..10522))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10285..10304
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10523..10627)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtccgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta cgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacaccaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta ataaccccgc cccgttgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagaatt
      841 ttgtaatacg actcactata gggcggccgc ataacttcgt atagcataca ttatacgaag
      901 ttatggatca ggccaaatcg gccgagctcg aattcgtcga gagcggagac gggagtttct
      961 cctcggggtc ggagcaggag gcacgcggag tgtgaggcca cgcatgagcg gacgctaacc
     1021 ccctccccag ccacaaagag tctacatgtc tagggtctag acatgttcag ctttgtggac
     1081 ctccggctcc tgctcctctt agcggccacc gccctcctga cgcacggcca agaggatatc
     1141 gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc
     1201 gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc
     1261 aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc
     1321 gtgaccaccc tgacctgggg cgtgcagtgc ttcgcccgct accccgacca catgaagcag
     1381 cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc
     1441 aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg
     1501 aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag
     1561 ctggagtaca acgccatcag cgacaacgtc tatatcaccg ccgacaagca gaagaacggc
     1621 atcaaggcca acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac
     1681 cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac
     1741 ctgagcaccc agtccaagct gagcaaagac cccaacgaga agcgcgatca catggtcctg
     1801 ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta cgaccaagaa
     1861 accaccggcg tcgagggacc caagggagac actggccccc gaggcccaag gggacccgca
     1921 ggcccccctg gccgagatgg catccctgga cagcctggac ttcccggacc ccccggaccc
     1981 cccggacctc ccggaccccc tggcctcgga ggaaactttg ctccccagct gtcttatggc
     2041 tatgatgaga aatcaaccgg aggaatttcc gtgcctggcc ccatgggtcc ctctggtcct
     2101 cgtggtctcc ctggcccccc tggtgcacct ggtccccaag gcttccaagg tccccctggt
     2161 gagcctggcg agcctggagc ttcaggtccc atgggtcccc gaggtccccc aggtccccct
     2221 ggaaagaatg gagatgatgg ggaagctgga aaacctggtc gtcctggtga gcgtgggcct
     2281 cctgggcctc agggtgctcg aggattgccc ggaacagctg gcctccctgg aatgaaggga
     2341 cacagaggtt tcagtggttt ggatggtgcc aagggagatg ctggtcctgc tggtcctaag
     2401 ggtgagcctg gcagccctgg tgaaaatgga gctcctggtc agatgggccc ccgtggcctg
     2461 cctggtgaga gaggtcgccc tggagcccct ggccctgctg gtgctcgtgg aaatgatggt
     2521 gctactggtg ctgccgggcc ccctggtccc accggccccg ctggtcctcc tggcttccct
     2581 ggtgctgttg gtgctaaggg tgaagctggt ccccaagggc cccgaggctc tgaaggtccc
     2641 cagggtgtgc gtggtgagcc tggcccccct ggccctgctg gtgctgctgg ccctgctgga
     2701 aaccctggtg ctgatggaca gcctggtgct aaaggtgcca atggtgctcc tggtattgct
     2761 ggtgctcctg gcttccctgg tgcccgaggc ccctctggac cccagggccc cggcggccct
     2821 cctggtccca agggtaacag cggtgaacct ggtgctcctg gcagcaaagg agacactggt
     2881 gctaagggag agcctggccc tgttggtgtt caaggacccc ctggccctgc tggagaggaa
     2941 ggaaagcgag gagctcgagg tgaacccgga cccactggcc tgcccggacc ccctggcgag
     3001 cgtggtggac ctggtagccg tggtttccct ggcgcagatg gtgttgctgg tcccaagggt
     3061 cccgctggtg aacgtggttc tcctggccct gctggcccca aaggatctcc tggtgaagct
     3121 ggtcgtcccg gtgaagctgg tctgcctggt gccaagggtc tgactggaag ccctggcagc
     3181 cctggtcctg atggcaaaac tggcccccct ggtcccgccg gtcaagatgg tcgccccgga
     3241 cccccaggcc cacctggtgc ccgtggtcag gctggtgtga tgggattccc tggacctaaa
     3301 ggtgctgctg gagagcccgg caaggctgga gagcgaggtg ttcccggacc ccctggcgct
     3361 gtcggtcctg ctggcaaaga tggagaggct ggagctcagg gaccccctgg ccctgctggt
     3421 cccgctggcg agagaggtga acaaggccct gctggctccc ccggattcca gggtctccct
     3481 ggtcctgctg gtcctccagg tgaagcaggc aaacctggtg aacagggtgt tcctggagac
     3541 cttggcgccc ctggcccctc tggagcaaga ggcgagagag gtttccctgg cgagcgtggt
     3601 gtgcaaggtc cccctggtcc tgctggtccc cgaggggcca acggtgctcc cggcaacgat
     3661 ggtgctaagg gtgatgctgg tgcccctgga gctcccggta gccagggcgc ccctggcctt
     3721 cagggaatgc ctggtgaacg tggtgcagct ggtcttccag ggcctaaggg tgacagaggt
     3781 gatgctggtc ccaaaggtgc tgatggctct cctggcaaag atggcgtccg tggtctgacc
     3841 ggccccattg gtcctcctgg ccctgctggt gcccctggtg acaagggtga aagtggtccc
     3901 agcggccctg ctggtcccac tggagctcgt ggtgcccccg gagaccgtgg tgagcctggt
     3961 ccccccggcc ctgctggctt tgctggcccc cctggtgctg acggccaacc tggtgctaaa
     4021 ggcgaacctg gtgatgctgg tgctaaaggc gatgctggtc cccctggccc tgccggaccc
     4081 gctggacccc ctggccccat tggtaatgtt ggtgctcctg gagccaaagg tgctcgcggc
     4141 agcgctggtc cccctggtgc tactggtttc cctggtgctg ctggccgagt cggtcctcct
     4201 ggcccctctg gaaatgctgg accccctggc cctcctggtc ctgctggcaa agaaggcggc
     4261 aaaggtcccc gtggtgagac tggccctgct ggacgtcctg gtgaagttgg tccccctggt
     4321 ccccctggcc ctgctggcga gaaaggatcc cctggtgctg atggtcctgc tggtgctcct
     4381 ggtactcccg ggcctcaagg tattgctgga cagcgtggtg tggtcggcct gcctggtcag
     4441 agaggagaga gaggcttccc tggtcttcct ggcccctctg gtgaacctgg caaacaaggt
     4501 ccctctggag caagtggtga acgtggtccc cctggtccca tgggcccccc tggattggct
     4561 ggaccccctg gtgaatctgg acgtgagggg gctcctggtg ccgaaggttc ccctggacga
     4621 gacggttctc ctggcgccaa gggtgaccgt ggtgagaccg gccccgctgg accccctggt
     4681 gctcctggtg ctcctggtgc ccctggcccc gttggccctg ctggcaagag tggtgatcgt
     4741 ggtgagactg gtcctgctgg tcccgccggt cctgtcggcc ctgttggcgc ccgtggcccc
     4801 gccggacccc aaggcccacg tggtgacaag ggtgagacag gcgaacaggg cgacagaggc
     4861 ataaagggtc accgtggctt ctctggcctc cagggtcccc ctggccctcc tggctctcct
     4921 ggtgaacaag gtccctctgg agcctctggt cctgctggtc cccgaggtcc ccctggctct
     4981 gctggtgctc ctggcaaaga tggactcaac ggtctccctg gccccattgg gccccctggt
     5041 cctcgcggtc gcactggtga tgctggtcct gttggtcccc ccggccctcc tggacctcct
     5101 ggtccccctg gtcctcccag cgctggtttc gacttcagct tcctgcccca gccacctcaa
     5161 gagaaggctc acgatggtgg ccgctactac cgggctgatg atgccaatgt ggttcgtgac
     5221 cgtgacctcg aggtggacac caccctcaag agcctgagcc agcagatcga gaacatccgg
     5281 agcccagagg gcagccgcaa gaaccccgcc cgcacctgcc gtgacctcaa gatgtgccac
     5341 tctgactgga agagtggaga gtactggatt gaccccaacc aaggctgcaa cctggatgcc
     5401 atcaaagtct tctgcaacat ggagactggt gagacctgcg tgtaccccac tcagcccagt
     5461 gtggcccaga agaactggta catcagcaag aaccccaagg acaagaggca tgtctggttc
     5521 ggcgagagca tgaccgatgg attccagttc gagtatggcg gccagggctc cgaccctgcc
     5581 gatgtggcca tccagctgac cttcctgcgc ctgatgtcca ccgaggcctc ccagaacatc
     5641 acctaccact gcaagaacag cgtggcctac atggaccagc agactggcaa cctcaagaag
     5701 gccctgctcc tccagggctc caacgagatc gagatccgcg ccgagggcaa cagccgcttc
     5761 acctacagcg tcactgtcga tggctgcacg agtcacaccg gagcctgggg caagacagtg
     5821 attgaataca aaaccaccaa gacctcccgc ctgcgcatca tcgatgtggc ccccttggac
     5881 gttggtgccc cagaccagga attcggcttc gacgttggcc atgtctgctt cctgtaaact
     5941 ccctccatcc caacctggct ccctcccacc caaccaactt tccccccaac ccggaaacag
     6001 acaagcaacc caaactgaac cccctcaaaa gccaaaaaat gggagacaat ttcacatgga
     6061 ctttggaaaa tatttttttc ctttgcattc atctctcaaa cttagttttt atctttgacc
     6121 aaccgaacat gaccaaaaac caaaagtgca ttcaacctta ccaaaaaaaa aaaaaaaact
     6181 ctccagcgct ggatcccctg gtgctgatgg tcctgctggt gctcctggta ctccccaaac
     6241 ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc
     6301 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga
     6361 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga
     6421 cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat
     6481 ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag
     6541 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     6601 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     6661 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     6721 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
     6781 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     6841 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
     6901 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt
     6961 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
     7021 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
     7081 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
     7141 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     7201 ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag
     7261 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata
     7321 tccattttcg gatctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat
     7381 ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca
     7441 caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg
     7501 gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg
     7561 cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact
     7621 gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct
     7681 caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg
     7741 cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt
     7801 actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc
     7861 gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc
     7921 gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga
     7981 ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc
     8041 cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt
     8101 atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga
     8161 gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt
     8221 tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg
     8281 gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt
     8341 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     8401 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     8461 tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg
     8521 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     8581 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     8641 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     8701 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     8761 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     8821 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     8881 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     8941 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     9001 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     9061 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     9121 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     9181 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     9241 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     9301 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     9361 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     9421 aacaaaccac cgctggtagc ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     9481 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     9541 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     9601 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     9661 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     9721 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     9781 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     9841 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     9901 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     9961 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
    10021 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
    10081 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
    10141 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
    10201 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
    10261 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
    10321 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
    10381 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
    10441 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
    10501 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
    10561 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
    10621 ttccgcgcac atttccccga aaagtgccac ctgacgtcta aacaaatagg ggttccgcgc
    10681 acatttcccc gaaaagtgcc acct
//
