LOCUS       sequence-271410-       10893 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentivirus for base editing activatable Luciferase2 expression in 
            mammalian cells. All in one vector with sgLuc2GO..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10893)
  AUTHORS   Katti A, Foronda M, Zimmerman J, Diaz B, Zafra MP, Goswami S, Dow LE
  TITLE     GO: a functional reporter system to identify and enrich base editing
            activity.
  JOURNAL   Nucleic Acids Res. 2020 Feb 29. pii: 5766653. doi: 
            10.1093/nar/gkaa124.
  PUBMED    32112097
REFERENCE   2  (bases 1 to 10893)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Feb 29. pii: 5766653. doi: 10.1093/nar/gkaa124."
COMMENT     Sequence Label: pLenti-mU6-Luc2GO-PGK-Neo
FEATURES             Location/Qualifiers
     source          1..10893
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1717..1965
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     1717..1737
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     1888..1907
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_feature    3991..4108
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4190..4503
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for mouse U6 snRNA (Das 
                     et al., 1988)"
     primer_bind     4422..4442
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     CDS             5075..6724
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="EDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDAH
                     IEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAPA
                     NDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQSM
                     YTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHAR
                     DPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYKI
                     QSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGYG
                     LTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMSG
                     YVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESILL
                     QHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVVF
                     VDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     misc_feature    6750..7338
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6803..6823)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7221..7232)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7410..7643
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7721..7842
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7758..7777)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7812..7831
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7882..8017
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8037..8056)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8038..8056)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8066..8083)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8066..8082)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8075..8097)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8224..8679
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8311..8330)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8521..8542
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8705..8809
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8810..8878,8879..9670)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9028..9047)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9841..10429
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10330..10349
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10583..10600
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10717..10738
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10753..10758,10759..10776,10777..10783)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10791..10807
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10796..10818
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10815..10831
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10815..10831
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10850..10870
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10852..10870
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccagagagg gcctatttcc catgattcct
     1741 tcatatttgc atatacgata caaggctgtt agagagataa ttagaattaa tttgactgta
     1801 aacacaaaga tattagtaca aaatacgtga cgtagaaagt aataatttct tgggtagttt
     1861 gcagttttaa aattatgttt taaaatggac tatcatatgc ttaccgtaac ttgaaagtat
     1921 ttcgatttct tggctttata tatcttgtgg aaaggacgaa acaccggaga cggttgtaaa
     1981 tgagcacaca aaatacacat gctaaaatat tatattctat gacctttata aaatcaacca
     2041 aaatcttctt tttaataact ttagtatcaa taattagaat ttttatgttc ctttttgcaa
     2101 acttttaata aaaatgagca aaataaaaaa acgctagttt tagtaactcg cgttgttttc
     2161 ttcaccttta ataatagcta ctccaccact tgttcctaag cggtcagctc ctgcttcaat
     2221 cattttttga gcatcttcaa atgttctaac tccaccagct gctttaacta aagcattgtc
     2281 tttaacaact gacttcatta gtttaacatc ttcaaatgtt gcacctgatt ttgaaaatcc
     2341 tgttgatgtt ttaacaaatt ctaatccagc ttcaacagct atttcacaag ctttcatgat
     2401 ttcttctttt gttaataaac aattttccat aatacattta acaacatgtg atccagctgc
     2461 tttttttaca gctttcatgt cttctaaaac taattcataa tttttgtctt ttaatgcacc
     2521 aatatttaat accatatcaa tttctgttgc accatcttta attgcttcag aaacttcgaa
     2581 tgcttttgta gctgttgtgc atgcacctag aggaaaacct acaacatttg ttattcctac
     2641 atttgtgcct tttaataatt ctttacaata gcttgttcaa tatgaattaa cacaaactgt
     2701 tgcaaaatca aattcaattg cttcatcaca taattgttta atttcagctt tcgtagcatc
     2761 ttgttttaat aatgtgtgat ctatatattt gtttagtttc attttttctc ctatatattc
     2821 atttttaatt ttaattcttt aataatttcg tctactttaa ctttagcgtt ttgaacagat
     2881 tcaccaacac ctataaaata aatttttagt ttaggttcag ttccacttgg gcgaacagca
     2941 aatcatgact tatcttctaa ataaaatttt agtaagtctt gtcctggcat attatacatt
     3001 ccatcgatgt agtcttcaac attaacaact ttaagtccag caatttgagt taagggtgtt
     3061 gctctcaatg atttcattaa tggttcaatt tttaatttct tttcttctgg tttaaaattc
     3121 aagtttaaag tgaaagtgta atatgcaccc atttctttaa ataaatcttc taaatagtct
     3181 actaatgttt tattttgttt tttataaaat caagcagcct ctgctattaa tatagaagct
     3241 tgtattccat ctttatctct agctgagtca tcaattacat atccataact ttcttcataa
     3301 gcaaaaacaa aatttaatcc gttatcttct tctttagcaa tttctctacc cattcattta
     3361 aatccagtta aagtttttac aatattaact ccatattttt catgagcgat tctatcaccc
     3421 aaatcacttg ttacaaaact tgaatataga gccggatttt ttggaatgct atttaagcgt
     3481 tttagatttg ataattttca atcaattaaa attggtcctg tttgatttcc atctaatctt
     3541 acaaaatgac catcatgttt tattgccatt ccaaatctgt cagcatctgg gtcattcata
     3601 ataataatat ctgcatcatg tttaatacca tattcaagcg gtatttttca tgcaggatca
     3661 aattctggat ttggatttac aacattttta aatgtttcat cttcaaatgc atgctcttca
     3721 acctcaataa cgttatatcc tgattcacgt aatatttttg gggtaaattt agttcctgtt
     3781 ccattaactg cgctaaaaat aatttttaaa tcttttttag cttcttgctc ttttttgtac
     3841 gtctctgttt aagagctatg ctggaaacag catagcaagt ttaaataagg ctagtccgtt
     3901 atcaactttg ctggaaacag caaagtggca ccgagtcggt gctttttttg aattctagat
     3961 cttgagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta
     4021 cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca
     4081 aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca gagatccact
     4141 ttggcgccgg ccgatcgcaa ttctgcagac aaatggctct agatctagag atccgacgcc
     4201 gccatctcta ggcccgcgcc ggccccctcg cacagacttg tgggagaagc tcggctactc
     4261 ccctgccccg gttaatttgc atataatatt tcctagtaac tatagaggct taatgtgcga
     4321 taaaagacag ataatctgtt ctttttaata ctagctacat tttacatgat aggcttggat
     4381 ttctataaga gatacaaata ctaaattatt attttaaaaa acagcacaaa aggaaactca
     4441 ccctaactgt aaagtaattg tgtgttttga gactataaat atgcatgcga gaaaagcctt
     4501 gtttgacgac ggtgagcaag ggcggtttaa gagctatgct ggaaacagca tagcaagttt
     4561 aaataaggct agtccgttat caacttgaaa aagtggcacc gagtcggtgc ttttttgttt
     4621 taagtctcga gatcgagggc tagcgactag ctgcagtaac gccattttgc aaggcatgga
     4681 aaaataccaa accaagaata gagaagttca gatcaagggc gggtacatga aaatagctaa
     4741 cgttgggcca aacaggatat ctgcggtgag cagtttcggc cccggcccgg ggccaagaac
     4801 agatggtcac cgcagtttcg gccccggccc gaggccaaga acagatggtc cccagatatg
     4861 gcccaaccct cagcagtttc ttaagaccca tcagatgttt ccaggctccc ccaaggacct
     4921 gaaatgaccc tgcgccttat ttgaattaac caatcagcct gcttctcgct tctgttcgcg
     4981 cgcttctgct tcccgagctc tataaaagag ctcacaaccc ctcactcggc gcgccagtcc
     5041 tccgacagaa ttcgcgacga cggtgagcaa gggcgaggat gccaaaaaca ttaagaaggg
     5101 cccagcgcca ttctacccac tcgaagatgg gaccgccggc gagcagctgc acaaagccat
     5161 gaagcgctac gccctggtgc ccggcaccat cgcctttacc gacgcacata tcgaggtgga
     5221 cattacctac gccgagtact tcgagatgag cgttcgactg gcagaagcta tgaagcgtta
     5281 tgggctgaat acaaaccatc ggatcgtggt gtgcagcgag aatagcttgc agttcttcat
     5341 gcccgtgttg ggtgccctgt tcatcggtgt ggctgtggcc ccagctaacg acatctacaa
     5401 cgagcgcgag ctgctgaaca gcatgggcat cagccagccc accgtcgtat tcgtgagcaa
     5461 gaaagggctg caaaagatcc tcaacgtgca aaagaagcta ccgatcatac aaaagatcat
     5521 catcatggat agcaagaccg actaccaggg cttccaaagc atgtacacct tcgtgacttc
     5581 ccatttgcca cccggcttca acgagtacga cttcgtgccc gagagcttcg accgggacaa
     5641 aaccatcgcc ctgatcatga acagtagtgg cagtaccgga ttgcccaagg gcgtagccct
     5701 accgcaccgc accgcttgtg tccgattcag tcatgcccgc gaccccatct tcggcaacca
     5761 gatcatcccc gacaccgcta tcctcagcgt ggtgccattt caccacggct tcggcatgtt
     5821 caccacgctg ggctacttga tctgcggctt tcgggtcgtg ctcatgtacc gctttgagga
     5881 agagctattc ttgcgcagct tacaagacta taagattcaa tctgccctgc tggtgcccac
     5941 actattcagc ttcttcgcta agagtactct catcgataag tacgacctaa gtaacttgca
     6001 cgagatcgcc agtggcgggg cgccgctcag caaggaagta ggtgaggccg tggccaaacg
     6061 cttccaccta ccaggcatcc gccagggcta cggcctgaca gaaacaacca gcgccattct
     6121 gatcaccccc gaaggggacg acaagcctgg cgcagtaggc aaggtggtgc ccttcttcga
     6181 ggctaaggtg gtggacttgg acaccggtaa gacactgggt gtgaaccagc gcggcgagct
     6241 gtgcgtccgt ggccccatga tcatgagcgg ctacgttaac aaccccgagg ctactaacgc
     6301 actcatcgac aaggatggct ggctgcacag cggcgacatc gcctactggg acgaggacga
     6361 gcacttcttc atcgtggacc ggctgaagag cctgatcaaa tacaagggct accaggtagc
     6421 cccagccgaa ctggagagca tcctgctgca acaccccaac atcttcgacg ccggggtcgc
     6481 cggcctgccc gacgacgatg ccggcgagct gcccgccgca gtcgtcgtgc tggaacacgg
     6541 taaaaccatg accgagaagg agatcgtgga ctatgtggcc agccaggtta caaccgccaa
     6601 gaagctgcgc ggtggtgttg tgttcgtgga cgaggtgcct aaaggactga ccggcaagtt
     6661 ggacgcccgc aagatccgcg agattctcat taaggccaag aagggcggca agatcgccgt
     6721 gtaataaacg cgtagcgcgt taagtcgaca atcaacctct ggattacaaa atttgtgaaa
     6781 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
     6841 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
     6901 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt
     6961 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc
     7021 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc
     7081 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg
     7141 ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga
     7201 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc
     7261 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc
     7321 tttgggccgc ctccccgcgt cgactttaag accaatgact tacaaggcag ctgtagatct
     7381 tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca
     7441 agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct
     7501 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     7561 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     7621 gtcagtgtgg aaaatctcta gcagtacgta tagtagttca tgtcatctta ttattcagta
     7681 tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta
     7741 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     7801 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct
     7861 atcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     7921 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
     7981 ctattccaga agtagtgagg aggctttttt ggaggcctag ggacgtaccc aattcgccct
     8041 atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa
     8101 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
     8161 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
     8221 gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     8281 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     8341 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     8401 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
     8461 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     8521 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     8581 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     8641 ttaacgcgaa ttttaacaaa atattaacgc ttacaattta ggtggcactt ttcggggaaa
     8701 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     8761 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     8821 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     8881 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     8941 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     9001 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     9061 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     9121 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     9181 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     9241 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     9301 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     9361 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     9421 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     9481 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     9541 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     9601 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     9661 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     9721 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     9781 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     9841 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     9901 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     9961 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
    10021 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
    10081 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
    10141 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
    10201 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
    10261 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
    10321 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
    10381 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
    10441 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
    10501 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
    10561 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
    10621 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
    10681 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
    10741 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
    10801 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
    10861 tcactaaagg gaacaaaagc tggagctgca agc
//
