LOCUS       sequence-271421-        8741 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentivirus for base editing activatable GFP expression in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8741)
  AUTHORS   Katti A, Foronda M, Zimmerman J, Diaz B, Zafra MP, Goswami S, Dow LE
  TITLE     GO: a functional reporter system to identify and enrich base editing
            activity.
  JOURNAL   Nucleic Acids Res. 2020 Feb 29. pii: 5766653. doi: 
            10.1093/nar/gkaa124.
  PUBMED    32112097
REFERENCE   2  (bases 1 to 8741)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Feb 29. pii: 5766653. doi: 10.1093/nar/gkaa124."
COMMENT     Sequence Label: pRRL-GFPGO-PGK-Neo
FEATURES             Location/Qualifiers
     source          1..8741
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1806..1923
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             join(2368..2370,2371..3084)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(2368..2370,2371..3081)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(2410..2431)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2671..2690)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3018..3039
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        3119..3618
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3154..3177)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     3551..3570
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             3632..4435
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3695..3714)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4305..4324
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    4469..5057
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4522..4542)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4940..4951)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5264..5497
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5569..5690
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5606..5625)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5660..5679
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5730..5865
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5885..5904)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5886..5904)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5914..5931)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5914..5930)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5923..5945)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6072..6527
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6159..6178)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6369..6390
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6553..6657
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6658..6726,6727..7518)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6876..6895)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7689..8277
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8178..8197
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8431..8448
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8565..8586
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8601..8606,8607..8624,8625..8631)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8639..8655
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8644..8666
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8663..8679
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8663..8679
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8698..8718
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8700..8718
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
     1741 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcgg
     1801 ttaactttta aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac
     1861 ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat
     1921 tttatcgatc acgagactag cctcgatcga gctagctgca gtaacgccat tttgcaaggc
     1981 atggaaaaat accaaaccaa gaatagagaa gttcagatca agggcgggta catgaaaata
     2041 gctaacgttg ggccaaacag gatatctgcg gtgagcagtt tcggccccgg cccggggcca
     2101 agaacagatg gtcaccgcag tttcggcccc ggcccgaggc caagaacaga tggtccccag
     2161 atatggccca accctcagca gtttcttaag acccatcaga tgtttccagg ctcccccaag
     2221 gacctgaaat gaccctgcgc cttatttgaa ttaaccaatc agcctgcttc tcgcttctgt
     2281 tcgcgcgctt ctgcttcccg agctctataa aagagctcac aacccctcac tcggcgcgcc
     2341 agtcctccga cagaattcgc gacgacggtg agcaagggcg aggagctgtt caccggggtg
     2401 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc
     2461 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc
     2521 aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc
     2581 agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
     2641 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
     2701 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
     2761 gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat
     2821 atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc
     2881 gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
     2941 cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc
     3001 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc
     3061 ggcatggacg agctgtacaa gtaatgacgt atgcatgcaa ttgacgcgta attctaccgg
     3121 gtaggggagg cgcttttccc aaggcagtct ggagcatgcg ctttagcagc cccgctgggc
     3181 acttggcgct acacaagtgg cctctggcct cgcacacatt ccacatccac cggtaggcgc
     3241 caaccggctc cgttctttgg tggccccttc gcgccacctt ctactcctcc cctagtcagg
     3301 aagttccccc ccgccccgca gctcgcgtcg tgcaggacgt gacaaatgga agtagcacgt
     3361 ctcactagtc tcgtgcagat ggacagcacc gctgagcaat ggaagcgggt aggcctttgg
     3421 ggcagcggcc aatagcagct ttgctccttc gctttctggg ctcagaggct gggaaggggt
     3481 gggtccgggg gcgggctcag gggcgggctc aggggcgggg cgggcgcccg aaggtcctcc
     3541 ggaggcccgg cattctgcac gcttcaaaag cgcacgtctg ccgcgctgtt ctcctcttcc
     3601 tcatctccgg gcctttcgac ctgcagccaa tatgggatcg gccattgaac aagatggatt
     3661 gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca
     3721 gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct
     3781 ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg cagcgcggct
     3841 atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc
     3901 gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct
     3961 tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga
     4021 tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg
     4081 gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc
     4141 agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac
     4201 ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat
     4261 cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga
     4321 tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc
     4381 cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagggga
     4441 tccgtcgact tcgaaacgcg tctggaacaa tcaacctctg gattacaaaa tttgtgaaag
     4501 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
     4561 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
     4621 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
     4681 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
     4741 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
     4801 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
     4861 gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac
     4921 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
     4981 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct
     5041 ttgggccgcc tccccgcctg gaattaattc tgcagtcgag acctagaaaa acatggagca
     5101 atcacaagta gcaatacagc agctaccaat gctgattgtg cctggctaga agcacaagag
     5161 gaggaggagg tgggttttcc agtcacacct caggtacctt taagaccaat gacttacaag
     5221 gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac
     5281 tcccaacgaa gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc
     5341 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     5401 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     5461 ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt
     5521 attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt
     5581 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     5641 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
     5701 ctctagctat cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     5761 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
     5821 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggg acgtacccaa
     5881 ttcgccctat agtgagtcgt attacgcgcg ctcactggcc gtcgttttac aacgtcgtga
     5941 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag
     6001 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa
     6061 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     6121 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     6181 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     6241 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     6301 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     6361 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
     6421 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     6481 acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt
     6541 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     6601 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     6661 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     6721 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     6781 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     6841 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     6901 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     6961 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     7021 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     7081 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     7141 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     7201 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     7261 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     7321 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     7381 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     7441 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     7501 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     7561 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     7621 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     7681 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     7741 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     7801 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     7861 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     7921 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     7981 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     8041 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     8101 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     8161 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     8221 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     8281 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     8341 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     8401 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     8461 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
     8521 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc
     8581 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt
     8641 gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc caagcgcgca
     8701 attaaccctc actaaaggga acaaaagctg gagctgcaag c
//
