LOCUS       sequence-271422-        8522 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Env gene from HIV patient plasma in pcDNA3.1 expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8522)
  AUTHORS   Joshi A, Sedano M, Beauchamp B, Punke EB, Mulla ZD, Meza A, Alozie 
            OK, Mukherjee D, Garg H
  TITLE     HIV-1 Env Glycoprotein Phenotype along with Immune Activation 
            Determines CD4 T Cell Loss in HIV Patients.
  JOURNAL   J Immunol. 2016 Feb 15;196(4):1768-79. doi: 
            10.4049/jimmunol.1501588. Epub 2016 Jan 13.
  PUBMED    26764036
REFERENCE   2  (bases 1 to 8522)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.4049/jimmunol.1501588"; journalName: "J Immunol"; date: 
            "2016-02-15- 15"; volume: "196"; issue: "4"; pages: "1768-79"
COMMENT     Sequence Label: pHP051Env
FEATURES             Location/Qualifiers
     source          1..8522
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        144..523
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        524..727
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     678..698
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     772..791
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        772..790
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    2696..2929
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3114..3158
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             3455..3481
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     CDS             3929..3970
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             3980..3997
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(4020..4037)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4026..4250
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4296..4724
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4383..4402)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4593..4614
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4733..4753)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4738..5067
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4918..5053
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4980..4999
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5134..5928
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5188..5207)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5798..5817
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6104..6225
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6141..6160)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6195..6214
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6274..6290)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6274..6290)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6287..6309)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6298..6314
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6322..6328,6329..6346,6347..6352))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6367..6388
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6505..6522)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6676..7264)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6756..6775)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7435..8226,8227..8295))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8058..8077
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8296..8400)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8475..8494)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa caaggcaagg
       61 cttgaccgac aattgcatga agaatctgct tagggttagg cgttttgcgc tgcttcgcga
      121 tgtacgggcc agatatacgc gttgacattg attattgact agttattaat agtaatcaat
      181 tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa
      241 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt
      301 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta
      361 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt
      421 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc
      481 tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca
      541 gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat
      601 tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa
      661 caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag
      721 cagagctctc tggctaacta gagaacccac tgcttactgg cttatcgaaa ttaatacgac
      781 tcactatagg gagacccaag ctggctagtt aagcttggta ccgagctcgg atccagtacc
      841 cttcaccgat caagctttag gcatctccta tggcaggaag aagcggagac agcgacgaag
      901 aactcctcag gacagtcaga ctcatcaagc atctatacca aagcagtaag tagtaaatgt
      961 aatgctacca ttagtaatat tagcaatagt agcactagta gtagtagtaa taatagcaat
     1021 agttgtgtgg accatagtaa tcatagaata tagaaaatta ttaagacaaa gaagaataga
     1081 cagattaatt gatagaataa gagaaagagc agaagacagt ggcaatgaga gtgaagggga
     1141 tcaggaagca ttggcagcgc tggtggaaat ggggcatgat gctccttggg atgttgatga
     1201 tctgtgatgc cacagaaaaa ttgtgggtca cagtatatta tggggtacct gtgtggagag
     1261 aagcaaacgc tactctattt tgtgcatcag atgctaaagc atatgataca gaggcacaca
     1321 atgtttgggc tacatatgcc tgtgtaccca cagaccccaa cccacaagaa gtagtattgg
     1381 gaaatgtgac agaaaatttt aatatgtgga aaaatgacat ggtggaacag atgcataagg
     1441 atataatcag tctatgggat gaaagcctaa agccatgtgt aaaattaacc ccactctgtg
     1501 ttaccttaaa ttgcaccgat gatttaaata ttactagtac cacaaataat actgctggca
     1561 ccactggtaa taacaccact aggggtaacg aaggagagct aagaggagaa atgaaaaact
     1621 gctctttcaa tgtcaccaca aacatgcaag ataagaagca gaaagaatat gcgctttttt
     1681 ataaactgga tgtagaacca atagataata gtaataggag ttataggttg ataagttgta
     1741 atacctcagt tgttacacag gcctgtccaa aagtaaactt tgagccaatt cccatacatt
     1801 attgtgcccc ggctggtttt gcgattctaa agtgtaataa taaaatattt gatggaaagg
     1861 gaccatgtac aaatgtcagc acagtacaat gtacacatgg aattaggcca gtagtatcaa
     1921 ctcagctgct gttaaatggc agtctagcag aaaaggagat aatgattaga tctaaaaatt
     1981 tctcggacaa tgctgaaacc ataatagtac agttgaacaa gtctgtagta attaattgta
     2041 taagacccca caacaataca agaaaaagta taacttttgg accagggagg gcattttatg
     2101 cagcagacca aataacagga gatataagaa aagcacactg taacattagt gaagcagatt
     2161 ggtatgacac tttaaaacag gtagctgcaa aattaggaga aaaatttaat aaaacagcaa
     2221 tagtctttga caattcctca ggaggggacc cagaaattgc aatgcacagt tttaattgtg
     2281 gaggagaatt tttctactgt aatactacac cactgtttaa taaaatttgg tttaatagga
     2341 ctgactggtc taataatact gcagggtcaa ataccactaa cggcataatt acactccaat
     2401 gcaggataaa acaaattata aacatgtggc agggaatagg aaaagcaatg tatgcccctc
     2461 ccatcagagg acagattaaa tgtacatcaa acattacagg gctgctatta acaagagatg
     2521 gtggtaatcc ttcaaacgca accgatgaga tcttcagacc cataggggga aatatgaagg
     2581 acaattggag aagtgaatta tataaatata aagtagtaaa acttgaacca ataggactgg
     2641 cacccaccaa ggcgcagaga agagtggtgc agagagaaaa aagagcagtg ggactaggag
     2701 ctgtgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagtg tcaatgacgc
     2761 tgacggtaca ggccagacaa ttattgtctg gtatagtgca acagcaaagc aatttgctga
     2821 gggctattga ggcgcaacaa catatgttgc aactcacagt ctggggcatc aagcagctcc
     2881 aggcgagaat cctggctgtg gaaagatacc taaatgatca acagctcctg ggaatttggg
     2941 gttgctctgg gagacacatt tgcaccactg ctgtgccttg ggatactagt tggagtaata
     3001 aatctctgag tgccatctgg gagaacatga cctggatgga gtgggagaga gagatcagaa
     3061 atcacacaaa aacaatatat tccttaattg aagaatcgca gagccagcag gaaaagaatg
     3121 aacaggaatt attggaattg gataagtggg caagtttgtg gagttggttt gatataacaa
     3181 actggctgtg gtatataaga atattcataa tgatagtagg aggcttagta ggtttaagaa
     3241 tatttttcac tgtactttct atagtgaata gagttaggca gggatactca ccattatcgt
     3301 ttcagaccct cctcccagca gctcccaggg gacccgacag gcccgaagga atagaagaag
     3361 acggtggaga aagaaacaga gatggatccg gtccattagt gaatggattc ttagcactca
     3421 tttgggtcga cctgcggagc ctgtgcctct tcagctacca ccgcttgaga gacttactct
     3481 tgattgctgc gaggattgtg gaacttctgg gacgcagggg gtgggaagcc ctccaatatt
     3541 ggtggaacct cctgcagtat tggagtcagg aactaaagaa tagtgctatt agtttgttta
     3601 atgccacagc catagcagta gctgaaggga cagatagggg tatagaaata gtgcaaagag
     3661 ctggtagagc tattcgcaac atacctagaa ggataagaca gggcttagaa agatctttgc
     3721 tataagatgg gaggcaagtg gtcaaaacgt gtaactggat ggggatggcc ggctgtaagg
     3781 gaaaggatga gacgagctca accagcagag ccagcagcag aaggggtggg agcagtatct
     3841 cgagacggat ccagctaagg gtcaagacaa ttctgcagat atccagcaca gtggcggccg
     3901 ctcgagtcta gagggcccgc ggttcgaagg taagcctatc cctaaccctc tcctcggtct
     3961 cgattctacg cgtaccggtc atcatcacca tcaccattga gtttaaaccc gctgatcagc
     4021 ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt
     4081 gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca
     4141 ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga
     4201 ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc
     4261 ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag
     4321 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     4381 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     4441 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     4501 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     4561 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     4621 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta
     4681 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg
     4741 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     4801 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt
     4861 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
     4921 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     4981 atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc
     5041 ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga
     5101 tctgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca
     5161 ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc
     5221 ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc
     5281 aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg
     5341 ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg
     5401 gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct
     5461 gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct
     5521 acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa
     5581 gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa
     5641 ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt gacccatggc
     5701 gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt
     5761 ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct
     5821 gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc
     5881 gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg
     5941 ggttcgcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac
     6001 cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat
     6061 cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc
     6121 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     6181 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc
     6241 gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
     6301 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     6361 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     6421 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     6481 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     6541 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     6601 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     6661 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     6721 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     6781 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     6841 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     6901 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     6961 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     7021 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     7081 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     7141 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     7201 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     7261 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     7321 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     7381 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     7441 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     7501 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     7561 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     7621 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     7681 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     7741 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     7801 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     7861 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     7921 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     7981 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     8041 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     8101 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     8161 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     8221 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     8281 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     8341 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     8401 cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatctcc cgatccccta
     8461 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tctgctccct
     8521 gc
//
