LOCUS       sequence-271432-        8649 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Doxycycline-inducible vector backbone containing the VP64 activator 
            domain with FLAG and NLS sequences, followed by a multiple cloning 
            site (MCS).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8649)
  AUTHORS   Adrian-Segarra JM, Weigel B, Mall M
  TITLE     Combining Cell Fate Reprogramming and Protein Engineering to Study 
            Transcription Factor Functions.
  JOURNAL   Methods Mol Biol. 2021;2352:227-236. doi: 
            10.1007/978-1-0716-1601-7_15.
  PUBMED    34324190
REFERENCE   2  (bases 1 to 8649)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods Mol
            Biol."; date: "2021"; volume: "2352:227-236. doi"; pages: " 
            10.1007/978-1-0716-1601-7_15"
COMMENT     Sequence Label: TetO-FUW-FLAG-NLS-VP64-MCS_N-term
FEATURES             Location/Qualifiers
     source          1..8649
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      6..15
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             15..38
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             39..59
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             78..227
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     misc_feature    292..880
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(345..365)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(763..774)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(883..899)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(884..900)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             1405..1585
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(1611..1628)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1617..1841
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      1887..2315
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1974..1993)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2184..2205
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(2324..2344)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2329..2658
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2509..2644
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     2571..2590
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        2706..2753
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             2772..3146
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    3276..3397
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3313..3332)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3367..3386
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3446..3462)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3446..3462)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3459..3481)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3470..3486
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3494..3500,3501..3518,3519..3524))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3539..3560
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3677..3694)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3848..4436)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3928..3947)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4607..5398,5399..5467))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5230..5249
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5468..5572)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(5647..5666)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        5838..6217
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6219..6417
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             6435..6615
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6662..6787
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7280..7513
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7698..7742
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8040..8157
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(8214..8232,8233..8255,8256..8274,8275..8297,8298..8316,
                     8317..8339,8340..8358,8359..8381,8382..8400,8401..8423,
                     8424..8442,8443..8465,8466..8484)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        8517..8555
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8552..8576
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 aattggccac catggactac aaggacgacg atgacaagcc aaagaagaag cgtaaggtaa
       61 tgggttccgg acgggctgac gcattggacg attttgatct ggatatgctg ggaagtgacg
      121 ccctcgatga ttttgacctt gacatgcttg gttcggatgc ccttgatgac tttgacctcg
      181 acatgctcgg cagtgacgcc cttgatgatt tcgacctgga catgctgatt aacggatcct
      241 ctagagaaga ctattaatta agctagctaa ttcgatatca agcttatcga taatcaacct
      301 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
      361 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
      421 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
      481 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
      541 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
      601 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
      661 gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt
      721 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
      781 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
      841 cctcagacga gtcggatctc cctttgggcc gcctccccgc atcgataccg tcgacctcga
      901 gacctagaaa aacatggagc aatcacaagt agcaatacag cagctaccaa tgctgattgt
      961 gcctggctag aagcacaaga ggaggaggag gtgggttttc cagtcacacc tcaggtacct
     1021 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg
     1081 ggactggaag ggctaattca ctcccaacga agacaagata tccttgatct gtggatctac
     1141 cacacacaag gctacttccc tgattggcag aactacacac cagggccagg gatcagatat
     1201 ccactgacct ttggatggtg ctacaagcta gtaccagttg agcaagagaa ggtagaagaa
     1261 gccaatgaag gagagaacac ccgcttgtta caccctgtga gcctgcatgg gatggatgac
     1321 ccggagagag aagtattaga gtggaggttt gacagccgcc tagcatttca tcacatggcc
     1381 cgagagctgc atccggactg tactgggtct ctctggttag accagatctg agcctgggag
     1441 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     1501 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     1561 tagtcagtgt ggaaaatctc tagcagggcc cgtttaaacc cgctgatcag cctcgactgt
     1621 gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga
     1681 aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag
     1741 taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga
     1801 agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac
     1861 cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg
     1921 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     1981 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     2041 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     2101 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     2161 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     2221 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     2281 aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta
     2341 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     2401 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     2461 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
     2521 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     2581 gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     2641 ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcag
     2701 cacgtgttga caattaatca tcggcatagt atatcggcat agtataatac gacaaggtga
     2761 ggaactaaac catggccaag ttgaccagtg ccgttccggt gctcaccgcg cgcgacgtcg
     2821 ccggagcggt cgagttctgg accgaccggc tcgggttctc ccgggacttc gtggaggacg
     2881 acttcgccgg tgtggtccgg gacgacgtga ccctgttcat cagcgcggtc caggaccagg
     2941 tggtgccgga caacaccctg gcctgggtgt gggtgcgcgg cctggacgag ctgtacgccg
     3001 agtggtcgga ggtcgtgtcc acgaacttcc gggacgcctc cgggccggcc atgaccgaga
     3061 tcggcgagca gccgtggggg cgggagttcg ccctgcgcga cccggccggc aactgcgtgc
     3121 acttcgtggc cgaggagcag gactgacacg tgctacgaga tttcgattcc accgccgcct
     3181 tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc
     3241 gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg
     3301 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     3361 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct
     3421 ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     3481 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     3541 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     3601 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     3661 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     3721 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     3781 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     3841 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     3901 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     3961 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     4021 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     4081 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     4141 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     4201 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     4261 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     4321 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     4381 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     4441 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     4501 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     4561 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     4621 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     4681 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     4741 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     4801 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     4861 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     4921 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     4981 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     5041 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     5101 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     5161 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     5221 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     5281 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     5341 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     5401 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     5461 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     5521 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     5581 cccgaaaagt gccacctgac gtcgacggat cgggagatct cccgatcccc tatggtgcac
     5641 tctcagtaca atctgctctg atgccgcata gttaagccag tatctgctcc ctgcttgtgt
     5701 gttggaggtc gctgagtagt gcgcgagcaa aatttaagct acaacaaggc aaggcttgac
     5761 cgacaattgc atgaagaatc tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg
     5821 ggccagatat acgcgttgac attgattatt gactagttat taatagtaat caattacggg
     5881 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
     5941 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
     6001 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
     6061 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
     6121 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
     6181 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat
     6241 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
     6301 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc
     6361 cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagcgc
     6421 gttttgcctg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     6481 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     6541 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     6601 ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga
     6661 gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg
     6721 actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga
     6781 gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc
     6841 agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg
     6901 attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca
     6961 gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc
     7021 aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa
     7081 gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg ctgatcttca
     7141 gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag
     7201 taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag
     7261 aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca
     7321 ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag
     7381 tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca
     7441 cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg
     7501 atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc
     7561 cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga
     7621 tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat
     7681 cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt
     7741 tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag
     7801 taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta
     7861 ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccgaca
     7921 ggcccgaagg aatagaagaa gaaggtggag agagagacag agacagatcc attcgattag
     7981 tgaacggatc ggcactgcgt gcgccaattc tgcagacaaa tggcagtatt catccacaat
     8041 tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata
     8101 gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttcgg
     8161 gtttattaca gggacagcag agatccagtt tggttagatc tcgagtttac cactccctat
     8221 cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg
     8281 aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact
     8341 ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga
     8401 aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgagttt
     8461 accactccct atcagtgata gagaaaagtg aaagtcgagc tcggtacccg ggtcgaggta
     8521 ggcgtgtacg gtgggaggcc tatataagca gagctcgttt agtgaaccgt cagatcgcct
     8581 ggagacgcca tccacgctgt tttgacctcc atagaagaca ccgggaccga tccagcctcc
     8641 gcggccccg
//
