LOCUS       sequence-271436-        4405 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Template for generating in situ hybridization probe.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4405)
  AUTHORS   Toriba T, Tokunaga H, Nagasawa K, Nie F, Yoshida A, Kyozuka J
  TITLE     Suppression of Leaf Blade Development by BLADE-ON-PETIOLE Orthologs 
            Is a Common Strategy for Underground Rhizome Growth.
  JOURNAL   Curr Biol. 2020 Feb 3;30(3):509-516.e3. doi: 
            10.1016/j.cub.2019.11.055. Epub 2020 Jan 16.
  PUBMED    31956025
REFERENCE   2  (bases 1 to 4405)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2019.11.055"; journalName: "Curr Biol"; date: 
            "2020-02-3- 3"; volume: "30"; issue: "3"; pages: "509-516.e3"
COMMENT     Sequence Label: pGEM_ZmaBOP1binsitu
FEATURES             Location/Qualifiers
     source          1..4405
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..20
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(1547..1565)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1548..1565)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(1583..1599)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1583..1599)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1596..1618)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1607..1623
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1631..1637,1638..1655,1656..1661))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1676..1697
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1814..1831)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1985..2573)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2065..2084)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2744..3535,3536..3604))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3367..3386
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3605..3709)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3787..4242)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3924..3945)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4136..4155
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4368..4390
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4382..4399
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4383..4399
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 taatacgact cactataggg cgaattgggc ccttgagata caccatcctg ttgtccaagc
       61 tcagattcac catgcttgcg ttgttgccgc tggcattgaa ctcatggccg ccgccgccgg
      121 ggggctcacc gtacattggc gcggcattga cggctgcacc cgcggtgtga tgagcgtcct
      181 cgcgagacat gaccatggcc gccgactgca cgagctcgag gcagagccgg agcttgttgg
      241 gctcgatgtg cgccaggccc ggcagggcgc ccttgaagag gaagtcggag gtgagcgtgc
      301 ggaggatgtc gagcggcgtg acgccgtcga cggtgcgcac gttgggatcg gcgtggtggt
      361 cgagcagcac ggcgaccatg tcggggcaca ccatctcagc cgcgacatgc agcggcatct
      421 tcccggaggg acccgccggg tggttgacgt cggcggctcc gagctccagc agcgccttga
      481 cgacctcgcg gctgcagttc tcgacggcgt agtgcagcgc gagggcctcg tcgaggttga
      541 gcccctcgcc catgaccatg agcttgacga gctcgacgtc ggacgagtcg agcgcgcggc
      601 gcatgcggcg gatcttgtgc tggtcgtcga gctccaacgc agtcgacgag gcctccatcg
      661 tccccgccgg gtggtgctgc tggtggtggt ggtgcgcgag gaacggcgag cggcgggaca
      721 gcgaggagtt gtggcggagc tcgtcgatct tggcgaccac gtcgatgggc aggtgcttcg
      781 cgagcacctc cgccggcagg cccgacttgg cgacgaggtg ggagcaggtg gtccagagct
      841 ggtgcaggtc ttgcttccgg gacgccatca gcaccttcat cacgtcctcg atggacgcct
      901 tctccaccat gcccgccagc tgcttctgtg taaggagggc cagctcctcg acgccgaagg
      961 agcgggcggc ggcgagggtg tcgagcgcga ggtcgtccgc cgccgcgcag tgagtatgcc
     1021 agcacccgcg ctcgccgcac cccggccggg gctccccctt ctgcggcagg agggagacct
     1081 gcccgctgta caggaactgt agcagcagca ggaacacctc gtagctcacc gagttcacgg
     1141 gaatcacagc gcccggcgcg gccgccgagc cgccggtgcc gcgcggggag gacccggacg
     1201 cgccggaggg ggagcgcggg ctgaggtggt cgaacaccag cgcgccgggc gccggcgttg
     1261 actggtcggc ggcggcgccg cagaagaact tgcggaagaa gaggctgcgc gcggcgagga
     1321 tgcagcggtg cgcgtgcacg gggcggccct ccacgttgaa cgtcacgtcg ctgaaggcct
     1381 gcccgttgat gagcaggttc agatagtcca tggacagcga tttcagcgtg tcgtccatgg
     1441 gcgccggcgt ctgagtctcc cactttgctc gatctcgaat tcgcggccgc ctgcaggtcg
     1501 accatatggg agagctccca acgcgttgga tgcatagctt gagtattcta tagtgtcacc
     1561 taaatagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
     1621 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
     1681 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     1741 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     1801 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     1861 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     1921 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     1981 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     2041 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     2101 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     2161 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     2221 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     2281 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     2341 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     2401 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     2461 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     2521 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     2581 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     2641 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     2701 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     2761 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     2821 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     2881 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     2941 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     3001 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     3061 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     3121 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     3181 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     3241 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     3301 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     3361 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     3421 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     3481 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     3541 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     3601 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     3661 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     3721 gaaaagtgcc acctgatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc
     3781 atcaggaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
     3841 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
     3901 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
     3961 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
     4021 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
     4081 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
     4141 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa
     4201 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtccattcgc cattcaggct
     4261 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
     4321 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
     4381 ttgtaaaacg acggccagtg aattg
//
