LOCUS       sequence-271442-        9127 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression vector for constitutively active 
            dominant-negative HSF1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9127)
  AUTHORS   Moore CL, Dewal MB, Nekongo EE, Santiago S, Lu NB, Levine SS, 
            Shoulders MD
  TITLE     Transportable, Chemical Genetic Methodology for the Small 
            Molecule-Mediated Inhibition of Heat Shock Factor 1.
  JOURNAL   ACS Chem Biol. 2016 Jan 15;11(1):200-10. doi: 
            10.1021/acschembio.5b00740. Epub 2015 Nov 19.
  PUBMED    26502114
REFERENCE   2  (bases 1 to 9127)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acschembio.5b00740"; journalName: "ACS Chem Biol"; date: 
            "2016-01-15- 15"; volume: "11"; issue: "1"; pages: "200-10"
COMMENT     Sequence Label: pLenti CMV Puro dn-cHSF1
FEATURES             Location/Qualifiers
     source          1..9127
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     99..116
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    233..254
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(269..274,275..292,293..299)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    307..323
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     312..334
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     331..347
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     331..347
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     366..386
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        368..386
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        414..640
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             641..821
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    868..993
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1486..1719
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1904..1948
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2215..2331
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2354..2657
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2658..2861
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2812..2832
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2858..2882
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2966..2990
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4145..4169)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    4200..4788
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4253..4273)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4671..4682)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        4802..5301
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4837..4860)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5234..5253
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5322..5921
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5322..5341)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5818..5838
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             6059..6292
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6364..6485
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6401..6420)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6455..6474
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6525..6660
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6680..6699)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6681..6699)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6709..6726)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6709..6725)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6718..6740)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6867..7322
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6954..6973)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7164..7185
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7348..7452
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7453..7521,7522..8313)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7671..7690)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8484..9072
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8973..8992
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
       61 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
      121 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
      181 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
      241 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
      301 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca
      361 agcgcgcaat taaccctcac taaagggaac aaaagctgga gctgcaagct taatgtagtc
      421 ttatgcaata ctcttgtagt cttgcaacat ggtaacgatg agttagcaac atgccttaca
      481 aggagagaaa aagcaccgtg catgccgatt ggtggaagta aggtggtacg atcgtgcctt
      541 attaggaagg caacagacgg gtctgacatg gattggacga accactgaat tgccgcattg
      601 cagagatatt gtatttaagt gcctagctcg atacataaac gggtctctct ggttagacca
      661 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
      721 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
      781 atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac
      841 ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg
      901 cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga
      961 ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg
     1021 cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata
     1081 gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca
     1141 gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa
     1201 cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata
     1261 aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc
     1321 gcacagcaag cggccgctga tcttcagacc tggaggagga gatatgaggg acaattggag
     1381 aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa
     1441 ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct
     1501 tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca
     1561 ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga
     1621 ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat
     1681 cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg
     1741 aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga
     1801 acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag
     1861 cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt
     1921 attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg
     1981 gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc
     2041 tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca
     2101 cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag gtggagagag
     2161 agacagagac agatccattc gattagtgaa cggatctcga cggtatcggt taacttttaa
     2221 aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac
     2281 agacatacaa actaaagaat tacaaaaaca aattacaaaa ttcaaaattt tatcgataag
     2341 cttgggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
     2401 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
     2461 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
     2521 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
     2581 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
     2641 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
     2701 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
     2761 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
     2821 gcggtaggcg tgtacggtgg gaggtctata taagcagagc tcgtttagtg aaccgtcaga
     2881 tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccga ctctagtcca
     2941 gtgtggtgga attctgcaga tatcaacaag tttgtacaaa aaagcaggct ttaaaggaac
     3001 caattcagtc gactggatcc ggtaccacca tggatctgcc cgtgggcccc ggcgcggcgg
     3061 ggcccagcaa cgtcccggcc ttcctgacca agctgtggac cctcgtgagc gacccggaca
     3121 ccgacgcgct catctgctgg agcccgagcg ggaacagctt ccacgtgttc gaccagggcc
     3181 agtttgccaa ggaggtgctg cccaagtact tcaagcacaa caacatggcc agcttcgtgc
     3241 ggcagctcaa catgtatggc ttccggaaag tggtccacat cgagcagggc ggcctggtca
     3301 agccagagag agacgacacg gagttccagc acccatgctt cctgcgtggc caggagcagc
     3361 tccttgagaa catcaagagg aaagtgacca gtgtgtccac cctgaagagt gaagacataa
     3421 agatccgcca ggacagcgtc accaagctgc tgacggacgt gcagctgatg aaggggaagc
     3481 aggagtgcat ggactccaag ctcctggcca tgaagcatga gaatgaggct ctgtggcggg
     3541 aggtggccag ccttcggcag aagcatgccc agcaacagaa agtcatcctg ggggtgaaga
     3601 gaaagatccc cctgatgctg aacgacagtg gctcagcaca ttccatgccc aagtatagcc
     3661 ggcagttctc cctggagcac gtccacggct cgggccccta ctcggccccc tccccagcct
     3721 acagcagctc cagcctctac gcccctgatg ctgtggccag ctctggaccc atcatctccg
     3781 acatcaccga gctggctcct gccagcccca tggcctcccc cggcgggagc atagacgaga
     3841 ggcccctatc cagcagcccc ctggtgcgtg tcaaggagga gccccccagc ccgcctcaga
     3901 gcccccgggt agaggaggcg agtcccgggc gcccatcttc cgtggacacc ctcttgtccc
     3961 cgaccgccct cattgactcc atcctgcggg agagtgaacc tgcccccgcc tccgtcacag
     4021 ccctcacgga cgccaggggc cacacggaca ccgagggccg gcctccctcc cccccgccca
     4081 cctccacccc tgaaaagtgc ctcagcgtag cctgcctgta ggcggccgca ctcgagatat
     4141 ctagacccag ctttcttgta caaagtggtt gatatccagc acagtggcgg ccgctcgaca
     4201 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
     4261 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
     4321 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
     4381 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg
     4441 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta
     4501 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
     4561 tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg
     4621 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca
     4681 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc
     4741 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct ggaattctac
     4801 cgggtagggg aggcgctttt cccaaggcag tctggagcat gcgctttagc agccccgctg
     4861 ggcacttggc gctacacaag tggcctctgg cctcgcacac attccacatc caccggtagg
     4921 cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac cttctactcc tcccctagtc
     4981 aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat ggaagtagca
     5041 cgtctcacta gtctcgtgca gatggacagc accgctgagc aatggaagcg ggtaggcctt
     5101 tggggcagcg gccaatagca gctttgctcc ttcgctttct gggctcagag gctgggaagg
     5161 ggtgggtccg ggggcgggct caggggcggg ctcaggggcg gggcgggcgc ccgaaggtcc
     5221 tccggaggcc cggcattctg cacgcttcaa aagcgcacgt ctgccgcgct gttctcctct
     5281 tcctcatctc cgggcctttc gacctgcagc ccaagcttac catgaccgag tacaagccca
     5341 cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt
     5401 tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc gagcgggtca
     5461 ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg
     5521 cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg
     5581 tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc
     5641 aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca
     5701 ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg
     5761 gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca
     5821 acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag
     5881 gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg acgcccgccc cacgacccgc
     5941 agcgcccgac cgaaaggagc gcacgacccc atgcatctcg agggcccggt acctttaaga
     6001 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg
     6061 gaagggctag ctcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc
     6121 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     6181 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     6241 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt
     6301 tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag
     6361 aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     6421 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     6481 tcttatcatg tctggctcta gctatcccgc ccctaactcc gcccatcccg cccctaactc
     6541 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     6601 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     6661 tagggacgta cccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt
     6721 tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca
     6781 tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca
     6841 gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg
     6901 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     6961 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     7021 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     7081 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     7141 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     7201 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     7261 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat
     7321 ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     7381 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     7441 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     7501 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     7561 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     7621 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     7681 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     7741 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     7801 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     7861 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     7921 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     7981 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     8041 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     8101 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     8161 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     8221 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     8281 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     8341 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     8401 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     8461 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     8521 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     8581 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     8641 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     8701 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     8761 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     8821 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     8881 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     8941 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     9001 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     9061 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     9121 tttgctc
//
