LOCUS       sequence-271454-       10502 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Quantitative measurement of CArG response element activity. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10502)
  AUTHORS   Mistriotis P, Bajpai VK, Wang X, Rong N, Shahini A, Asmani M, Liang 
            MS, Wang J, Lei P, Liu S, Zhao R, Andreadis ST
  TITLE     NANOG Reverses the Myogenic Differentiation Potential of Senescent 
            Stem Cells by Restoring ACTIN Filamentous Organization and 
            SRF-Dependent Gene Expression.
  JOURNAL   Stem Cells. 2017 Jan;35(1):207-221. doi: 10.1002/stem.2452. Epub 
            2016 Jul 11.
  PUBMED    27350449
REFERENCE   2  (bases 1 to 10502)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/stem.2452";
            journalName: "Stem Cells"; date: "2017-01"; volume: "35"; issue: 
            "1"; pages: "207-221"
COMMENT     Sequence Label: LVDP-CArG-RE-GPR
FEATURES             Location/Qualifiers
     source          1..10502
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        382..580
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             598..778
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    825..950
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1443..1676
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1861..1905
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             complement(2430..3107)
                     /codon_start=1
                     /product="noncytotoxic tetrameric variant of DsRed 
                     fluorescent protein (Strack et al., 2008)"
                     /label=DsRed-Express2
                     /note="mammalian codon-optimized"
                     /translation="MDSTENVIKPFMRFKVHMEGSVNGHEFEIEGEGEGKPYEGTQTAK
                     LQVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGTFIYHVKFIGVNFPSDGPVMQKKTLGWEPSTERLYPRDGVLKGEIHK
                     ALKLKGGGHYLVEFKSIYMAKKPVKLPGYYYVDSKLDITSHNEDYTVVEQYERAEARHH
                     LFQ"
     primer_bind     complement(2492..2515)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(2690..2709)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     2907..2927
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     promoter        complement(3125..3635)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3188..3206)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     polyA_signal    4530..4578
                     /note="synthetic polyadenylation signal"
     CDS             complement(4587..5282)
                     /codon_start=1
                     /product="Zoanthus green fluorescent protein"
                     /label=ZsGreen1
                     /note="mammalian codon-optimized"
                     /translation="MAQSKHGLTKEMTMKYRMEGCVDGHKFVITGEGIGYPFKGKQAIN
                     LCVVEGGPLPFAEDILSAAFMYGNRVFTEYPQDIVDYFKNSCPAGYTWDRSFLFEDGAV
                     CICNADITVSVEENCMYHESKFYGVNFPADGPVMKKMTDNWEPSCEKIIPVPKQGILKG
                     DVSMYLLLKDGGRLRCQFDTVYKAKSVPRKMPDWHFIQHKLTREDRSDAKNQKWHLTEH
                     AIASGSALP"
     regulatory      5279..5288
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(5379..5403)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(5400..5438)
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             6070..6250
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6276..6293)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6282..6506
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6552..6980
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6639..6658)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6849..6870
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6989..7009)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6994..7323
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7174..7309
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7236..7255
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7371..7418
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7437..7811
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7941..8062
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7978..7997)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8032..8051
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8111..8127)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8111..8127)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8124..8146)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8135..8151
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8159..8165,8166..8183,8184..8189))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8204..8225
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8342..8359)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8513..9101)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8593..8612)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9272..10063,10064..10132))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9895..9914
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10133..10237)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10312..10331)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg
      601 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
      661 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
      721 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
      781 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
      841 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
      901 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
      961 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     1021 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     1081 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     1141 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     1201 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     1261 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     1321 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     1381 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     1441 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     1501 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     1561 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     1621 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     1681 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     1741 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     1801 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     1861 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     1921 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     1981 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     2041 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     2101 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atcatcttta
     2161 atttctaata tatttagaat ctttaatttc taatatattt agaatcttta atttctaata
     2221 tatttagaat ctttaatttc taatatattt agaatcttta atttctaata tatttagaat
     2281 ctttaatttc taatatattt agaatcttta atttctaata tatttagaat ctttaatttc
     2341 taatatattt agaaggatct ccgcgtttat gaacaaacga cccaacaccc gtgggttttt
     2401 tctgtctttt tattgccgtc attctcgagc tactggaaca ggtggtggcg ggcctcggcg
     2461 cgctcgtact gctccaccac ggtgtagtcc tcgttgtggg aggtgatgtc cagcttggag
     2521 tccacgtagt agtagccggg cagcttcacg ggcttcttgg ccatgtagat tgacttgaac
     2581 tccaccaggt agtggccgcc gcccttcagc ttcagcgcct tgtggatctc gcccttcagc
     2641 acgccgtcgc gggggtacag gcgctcggtg gagggctccc agcccagagt cttcttctgc
     2701 attacggggc cgtcggaggg gaagttcacg ccgatgaact tcacgtggta gatgaaggtg
     2761 ccgtcctgca gggaggagtc ctgggtcacg gtcaccacgc cgccgtcctc gaagttcatc
     2821 acgcgctccc acttgaagcc ctcggggaag gacagcttct tgtagtcggg gatgtcggcg
     2881 gggtgcttca cgtacacctt ggagccgtac tggaactggg gggacaggat gtcccaggcg
     2941 aagggcaggg ggccgccctt ggtcacctgc agcttggcgg tctgggtgcc ctcgtagggc
     3001 ttgccctcgc cctcgccctc gatctcgaac tcgtggccgt tcacggagcc ctccatgtgc
     3061 accttgaagc gcatgaaggg cttgatgacg ttctcagtgc tatccatggt tgtgctagct
     3121 acagctgggg agagaggtcg gtgattcggt caacgaggga gccgactgcc gacgtgcgct
     3181 ccggaggctt gcagaatgcg gaacaccgcg cgggcaggaa cagggcccac actaccgccc
     3241 cacaccccgc ctcccgcacc gccccttccc ggccgctgct ctcggcgcgc cctgctgagc
     3301 agccgctatt ggccacagcc catcgcggtc ggcgcgctgc cattgctccc tggcgctgtc
     3361 cgtctgcgag ggtactagtg agacgtgcgg cttccgtttg tcacgtccgg cacgccgcga
     3421 accgcaagga accttcccga cttaggggcg gagcaggaag cgtcgccggg gggcccacaa
     3481 gggtagcggc gaagatccgg gtgacgctgc gaacggacgt gaagaatgtg cgagacccag
     3541 ggtcggcgcc gctgcgtttc ccggaaccac gcccagagca gccgcgtccc tgcgcaaacc
     3601 cagggctgcc ttggaaaagg cgcaacccca accccgtgga attggatccg tgacgcactg
     3661 aacaggttgc ccaaggaggc tgtggatgcc ccatccctgc aggcattcaa ggccaggctg
     3721 gatgtggctc tgggcagcct gggctgctgg ttggcgaccc tgcacatagc agggggttgg
     3781 agctgggtga gcactgtgct cctttgcaac ccaggccatt ctatgattct gtcattctaa
     3841 atctctcttt cagcctaaag ctttttcccc gtatcccccc aggtgtctgc aggctcaaag
     3901 agcagcgaga agcgttcaga ggaaagcgat cgcgtgccac cttccccgtg ccgggctgtc
     3961 cccgcacgct gccggctccg gctcggggat gcggggggag cgccggaccg gagcggagcc
     4021 ccgggcggct cgctgctgcc ccctagcgga agagggatgc aattacatcc ctgggggctt
     4081 tggcgggggg ctctccccgt gagctcccgc ggaaggttaa ttaactgaca gccacgatcc
     4141 cataggtgaa ggcaaaggcg tggcagctag gaacactcac tacaccccca ctcaagggaa
     4201 caccgtgccc agctaccctg agacagcccc actcccagga aatgcaggtg ccaaccagcc
     4261 ccagtgaggc atggcacctg agccagacac ccccaaatag tcccttccca cctcctcaag
     4321 catctgcact ctgggtgaac ccagaaaaac tggggtacct ggggagacat agaagggcca
     4381 ggccaggaac tccccaataa gcaggaacag agacctgacc cctgagcctc ccccaccctc
     4441 taaggctgct caatgtcaag gcaggaggga tctccacaca cctgcactct gggtaaggac
     4501 aagttggccc ccactgcccc acctctagac acacaaaaaa ccaacacaca gatctaatga
     4561 aaataaagat cttttattgc tagaagttag ggcaaggcgg agccggaggc gatggcgtgc
     4621 tcggtcaggt gccacttctg gttcttggcg tcgctgcggt cctcgcgggt cagcttgtgc
     4681 tggatgaagt gccagtcggg catcttgcgg ggcacggact tggccttgta cacggtgtcg
     4741 aactggcagc gcaagcggcc accgtccttc agcagcaggt acatgctcac gtcgcccttc
     4801 aagatgccct gcttgggcac ggggatgatc ttctcgcagg agggctccca gttgtcggtc
     4861 atcttcttca tcacggggcc gtcggcgggg aagttcacgc cgtagaactt ggactcgtgg
     4921 tacatgcagt tctcctccac gctcacggtg atgtcggcgt tgcagatgca cacggcgccg
     4981 tcctcgaaca ggaaggagcg gtcccaggtg tagccggcgg ggcaggagtt cttgaagtag
     5041 tcgacgatgt cctgggggta ctcggtgaac acgcggttgc cgtacatgaa ggcggcggac
     5101 aagatgtcct cggcgaaggg caaggggccg ccctccacca cgcacaggtt gatggcctgc
     5161 ttgcccttga aggggtagcc gatgccctcg ccggtgatca cgaacttgtg gccgtccacg
     5221 cagccctcca tgcggtactt catggtcatc tccttggtca ggccgtgctt ggactgggcc
     5281 atggtggcgg atggcgcgcc taccggtgga tcccggaggc tggatcggtc ccggtgtctt
     5341 ctatggaggt caaaacagcg tggatggcgt ctccaggcga tctgacggtt cactaaacga
     5401 gctctgctta tataggcctc ccaccgtaca cgcctacctc gacccgggta ccgtcgactg
     5461 cagaattcga accttttatg gccttttatg gccttttatg gccttttatg gccttttatg
     5521 gccttttatg ggttaactgc gatcgcaagc gctagaattc ttcgagacct agaaaaacat
     5581 ggagcaatca caagtagcaa tacagcagct accaatgctg attgtgcctg gctagaagca
     5641 caagaggagg aggaggtggg ttttccagtc acacctcagg tacctttaag accaatgact
     5701 tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta
     5761 attcactccc aacgaagaca agatatcctt gatctgtgga tctaccacac acaaggctac
     5821 ttccctgatt ggcagaacta cacaccaggg ccagggatca gatatccact gacctttgga
     5881 tggtgctaca agctagtacc agttgagcaa gagaaggtag aagaagccaa tgaaggagag
     5941 aacacccgct tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagta
     6001 ttagagtgga ggtttgacag ccgcctagca tttcatcaca tggcccgaga gctgcatccg
     6061 gactgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     6121 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     6181 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     6241 atctctagca gggcccgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca
     6301 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac
     6361 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat
     6421 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca
     6481 tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag
     6541 ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     6601 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     6661 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     6721 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     6781 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     6841 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
     6901 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     6961 acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc
     7021 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg
     7081 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     7141 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     7201 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     7261 tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
     7321 aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcagcacgt gttgacaatt
     7381 aatcatcggc atagtatatc ggcatagtat aatacgacaa ggtgaggaac taaaccatgg
     7441 ccaagttgac cagtgccgtt ccggtgctca ccgcgcgcga cgtcgccgga gcggtcgagt
     7501 tctggaccga ccggctcggg ttctcccggg acttcgtgga ggacgacttc gccggtgtgg
     7561 tccgggacga cgtgaccctg ttcatcagcg cggtccagga ccaggtggtg ccggacaaca
     7621 ccctggcctg ggtgtgggtg cgcggcctgg acgagctgta cgccgagtgg tcggaggtcg
     7681 tgtccacgaa cttccgggac gcctccgggc cggccatgac cgagatcggc gagcagccgt
     7741 gggggcggga gttcgccctg cgcgacccgg ccggcaactg cgtgcacttc gtggccgagg
     7801 agcaggactg acacgtgcta cgagatttcg attccaccgc cgccttctat gaaaggttgg
     7861 gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc
     7921 tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca
     7981 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     8041 ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg
     8101 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     8161 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     8221 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     8281 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     8341 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     8401 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     8461 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     8521 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     8581 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     8641 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     8701 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     8761 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     8821 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     8881 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     8941 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     9001 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     9061 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     9121 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     9181 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     9241 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     9301 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     9361 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     9421 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     9481 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     9541 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     9601 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     9661 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     9721 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     9781 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     9841 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     9901 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     9961 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
    10021 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
    10081 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
    10141 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
    10201 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
    10261 ctgacgtcga cggatcggga gatctcccga tcccctatgg tgcactctca gtacaatctg
    10321 ctctgatgcc gcatagttaa gccagtatct gctccctgct tgtgtgttgg aggtcgctga
    10381 gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc ttgaccgaca attgcatgaa
    10441 gaatctgctt agggttaggc gttttgcgct gcttcgcgat gtacgggcca gatatacgcg
    10501 tt
//
