LOCUS       sequence-271457-       14427 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  3rd generation lentiviral plasmid for expression of Sp 
            dCas9-hTET1(CD) fusion protein in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14427)
  AUTHORS   Taghbalout A, Du M, Jillette N, Rosikiewicz W, Rath A, Heinen CD, Li
            S, Cheng AW
  TITLE     Enhanced CRISPR-based DNA demethylation by Casilio-ME-mediated 
            RNA-guided coupling of methylcytosine oxidation and DNA repair 
            pathways.
  JOURNAL   Nat Commun. 2019 Sep 20;10(1):4296. doi: 10.1038/s41467-019-12339-7.
  PUBMED    31541098
REFERENCE   2  (bases 1 to 14427)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-09-20"; volume: "10(1):4296. doi"; pages: " 
            10.1038/s41467-019-12339-7"
COMMENT     Sequence Label: pAT890_CMV/CAG_dCas9-hTET1(CD)
FEATURES             Location/Qualifiers
     source          1..14427
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1010..1030
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1012..1030
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1058..1284
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1285..1465
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1512..1637
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2130..2363
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2548..2592
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2767..3267
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     3186..3204
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             3289..3888
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3289..3308)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3785..3805
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    3930..4047
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        4164..4467
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4468..4671
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4622..4642
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    4694..4718
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4799..4825
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             4829..4849
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(4859..4882,4883..4885,4886..7441,7442..7444,
                     7445..8959)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the N863A mutation in the HNH 
                     catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             join(4859..4882,4883..4885,4886..7372,7373..7375,
                     7376..8959)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             8963..8983
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    complement(11228..11252)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             11254..11295
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    11337..11925
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(11390..11410)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(11808..11819)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             11997..12230
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    12308..12429
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12345..12364)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     12399..12418
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      12469..12604
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(12624..12643)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(12625..12643)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(12653..12670)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(12653..12669)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12662..12684)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      12811..13266
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(12898..12917)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     13108..13129
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        13292..13396
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(13397..13465,13466..14257)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(13615..13634)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     1021 tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc aatactcttg
     1081 tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac
     1141 cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag
     1201 acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga tattgtattt
     1261 aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg agcctgggag
     1321 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     1381 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     1441 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga
     1501 aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg
     1561 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag
     1621 atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc
     1681 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg
     1741 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa
     1801 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata
     1861 atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag
     1921 ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg
     1981 ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa
     2041 tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg
     2101 gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca
     2161 gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg
     2221 tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg
     2281 ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga
     2341 tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc
     2401 actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac
     2461 acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta
     2521 attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa
     2581 tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc
     2641 ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg
     2701 aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg
     2761 ggacccttgc gccttttcca aggcagccct gggtttgcgc agggacgcgg ctgctctggg
     2821 cgtggttccg ggaaacgcag cggcgccgac cctgggtctc gcacattctt cacgtccgtt
     2881 cgcagcgtca cccggatctt cgccgctacc cttgtgggcc ccccggcgac gcttcctgct
     2941 ccgcccctaa gtcgggaagg ttccttgcgg ttcgcggcgt gccggacgtg acaaacggaa
     3001 gccgcacgtc tcactagtac cctcgcagac ggacagcgcc agggagcaat ggcagcgcgc
     3061 cgaccgcgat gggctgtggc caatagcggc tgctcagcag ggcgcgccga gagcagcggc
     3121 cgggaagggg cggtgcggga ggcggggtgt ggggcggtag tgtgggccct gttcctgccc
     3181 gcgcggtgtt ccgcattctg caagcctccg gagcgcacgt cggcagtcgg ctccctcgtt
     3241 gaccgaatca ccgacctctc tccccagggg gtaccaccgg agcttaccat gaccgagtac
     3301 aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc
     3361 gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag
     3421 cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg
     3481 tgggtcgcgg acgacggcgc cgccgtggcg gtctggacca cgccggagag cgtcgaagcg
     3541 ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc
     3601 gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc
     3661 ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg
     3721 ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg
     3781 ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg
     3841 cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgaga attctagatc
     3901 ttgagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat tggggggtac
     3961 agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa
     4021 aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag agatccactt
     4081 tggcgccggc tcgagggggc ccggggttat taatagtaat caattacggg gtcattagtt
     4141 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
     4201 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
     4261 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
     4321 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
     4381 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
     4441 tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt
     4501 ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt
     4561 ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg
     4621 acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc tctctggcta
     4681 actgtcggga tcaacaagtt tgtacaaaaa agcaggctcc gaattcaccg gtgccgccac
     4741 catgatcgat ggtggcggtg gtagcggggg aggcggctcc gggggcggag gcagtatgta
     4801 cccatacgat gttccagatt acgcttcgcc gaagaaaaag cgcaaggtcg aagcgtccga
     4861 caagaagtac agcatcggcc tggccatcgg caccaactct gtgggctggg ccgtgatcac
     4921 cgacgagtac aaggtgccca gcaagaaatt caaggtgctg ggcaacaccg accggcacag
     4981 catcaagaag aacctgatcg gagccctgct gttcgacagc ggcgaaacag ccgaggccac
     5041 ccggctgaag agaaccgcca gaagaagata caccagacgg aagaaccgga tctgctatct
     5101 gcaagagatc ttcagcaacg agatggccaa ggtggacgac agcttcttcc acagactgga
     5161 agagtccttc ctggtggaag aggataagaa gcacgagcgg caccccatct tcggcaacat
     5221 cgtggacgag gtggcctacc acgagaagta ccccaccatc taccacctga gaaagaaact
     5281 ggtggacagc accgacaagg ccgacctgcg gctgatctat ctggccctgg cccacatgat
     5341 caagttccgg ggccacttcc tgatcgaggg cgacctgaac cccgacaaca gcgacgtgga
     5401 caagctgttc atccagctgg tgcagaccta caaccagctg ttcgaggaaa accccatcaa
     5461 cgccagcggc gtggacgcca aggccatcct gtctgccaga ctgagcaaga gcagacggct
     5521 ggaaaatctg atcgcccagc tgcccggcga gaagaagaat ggcctgttcg gcaacctgat
     5581 tgccctgagc ctgggcctga cccccaactt caagagcaac ttcgacctgg ccgaggatgc
     5641 caaactgcag ctgagcaagg acacctacga cgacgacctg gacaacctgc tggcccagat
     5701 cggcgaccag tacgccgacc tgtttctggc cgccaagaac ctgtccgacg ccatcctgct
     5761 gagcgacatc ctgagagtga acaccgagat caccaaggcc cccctgagcg cctctatgat
     5821 caagagatac gacgagcacc accaggacct gaccctgctg aaagctctcg tgcggcagca
     5881 gctgcctgag aagtacaaag agattttctt cgaccagagc aagaacggct acgccggcta
     5941 cattgacggc ggagccagcc aggaagagtt ctacaagttc atcaagccca tcctggaaaa
     6001 gatggacggc accgaggaac tgctcgtgaa gctgaacaga gaggacctgc tgcggaagca
     6061 gcggaccttc gacaacggca gcatccccca ccagatccac ctgggagagc tgcacgccat
     6121 tctgcggcgg caggaagatt tttacccatt cctgaaggac aaccgggaaa agatcgagaa
     6181 gatcctgacc ttccgcatcc cctactacgt gggccctctg gccaggggaa acagcagatt
     6241 cgcctggatg accagaaaga gcgaggaaac catcaccccc tggaacttcg aggaagtggt
     6301 ggacaagggc gcttccgccc agagcttcat cgagcggatg accaacttcg ataagaacct
     6361 gcccaacgag aaggtgctgc ccaagcacag cctgctgtac gagtacttca ccgtgtataa
     6421 cgagctgacc aaagtgaaat acgtgaccga gggaatgaga aagcccgcct tcctgagcgg
     6481 cgagcagaaa aaggccatcg tggacctgct gttcaagacc aaccggaaag tgaccgtgaa
     6541 gcagctgaaa gaggactact tcaagaaaat cgagtgcttc gactccgtgg aaatctccgg
     6601 cgtggaagat cggttcaacg cctccctggg cacataccac gatctgctga aaattatcaa
     6661 ggacaaggac ttcctggaca atgaggaaaa cgaggacatt ctggaagata tcgtgctgac
     6721 cctgacactg tttgaggaca gagagatgat cgaggaacgg ctgaaaacct atgcccacct
     6781 gttcgacgac aaagtgatga agcagctgaa gcggcggaga tacaccggct ggggcaggct
     6841 gagccggaag ctgatcaacg gcatccggga caagcagtcc ggcaagacaa tcctggattt
     6901 cctgaagtcc gacggcttcg ccaacagaaa cttcatgcag ctgatccacg acgacagcct
     6961 gacctttaaa gaggacatcc agaaagccca ggtgtccggc cagggcgata gcctgcacga
     7021 gcacattgcc aatctggccg gcagccccgc cattaagaag ggcatcctgc agacagtgaa
     7081 ggtggtggac gagctcgtga aagtgatggg ccggcacaag cccgagaaca tcgtgatcga
     7141 aatggccaga gagaaccaga ccacccagaa gggacagaag aacagccgcg agagaatgaa
     7201 gcggatcgaa gagggcatca aagagctggg cagccagatc ctgaaagaac accccgtgga
     7261 aaacacccag ctgcagaacg agaagctgta cctgtactac ctgcagaatg ggcgggatat
     7321 gtacgtggac caggaactgg acatcaaccg gctgtccgac tacgatgtgg acgccatcgt
     7381 gcctcagagc tttctgaagg acgactccat cgacaacaag gtgctgacca gaagcgacaa
     7441 gaaccggggc aagagcgaca acgtgccctc cgaagaggtc gtgaagaaga tgaagaacta
     7501 ctggcggcag ctgctgaacg ccaagctgat tacccagaga aagttcgaca atctgaccaa
     7561 ggccgagaga ggcggcctga gcgaactgga taaggccggc ttcatcaaga gacagctggt
     7621 ggaaacccgg cagatcacaa agcacgtggc acagatcctg gactcccgga tgaacactaa
     7681 gtacgacgag aatgacaagc tgatccggga agtgaaagtg atcaccctga agtccaagct
     7741 ggtgtccgat ttccggaagg atttccagtt ttacaaagtg cgcgagatca acaactacca
     7801 ccacgcccac gacgcctacc tgaacgccgt cgtgggaacc gccctgatca aaaagtaccc
     7861 taagctggaa agcgagttcg tgtacggcga ctacaaggtg tacgacgtgc ggaagatgat
     7921 cgccaagagc gagcaggaaa tcggcaaggc taccgccaag tacttcttct acagcaacat
     7981 catgaacttt ttcaagaccg agattaccct ggccaacggc gagatccgga agcggcctct
     8041 gatcgagaca aacggcgaaa ccggggagat cgtgtgggat aagggccggg attttgccac
     8101 cgtgcggaaa gtgctgagca tgccccaagt gaatatcgtg aaaaagaccg aggtgcagac
     8161 aggcggcttc agcaaagagt ctatcctgcc caagaggaac agcgataagc tgatcgccag
     8221 aaagaaggac tgggacccta agaagtacgg cggcttcgac agccccaccg tggcctattc
     8281 tgtgctggtg gtggccaaag tggaaaaggg caagtccaag aaactgaaga gtgtgaaaga
     8341 gctgctgggg atcaccatca tggaaagaag cagcttcgag aagaatccca tcgactttct
     8401 ggaagccaag ggctacaaag aagtgaaaaa ggacctgatc atcaagctgc ctaagtactc
     8461 cctgttcgag ctggaaaacg gccggaagag aatgctggcc tctgccggcg aactgcagaa
     8521 gggaaacgaa ctggccctgc cctccaaata tgtgaacttc ctgtacctgg ccagccacta
     8581 tgagaagctg aagggctccc ccgaggataa tgagcagaaa cagctgtttg tggaacagca
     8641 caagcactac ctggacgaga tcatcgagca gatcagcgag ttctccaaga gagtgatcct
     8701 ggccgacgct aatctggaca aagtgctgtc cgcctacaac aagcaccggg ataagcccat
     8761 cagagagcag gccgagaata tcatccacct gtttaccctg accaatctgg gagcccctgc
     8821 cgccttcaag tactttgaca ccaccatcga ccggaagagg tacaccagca ccaaagaggt
     8881 gctggacgcc accctgatcc accagagcat caccggcctg tacgagacac ggatcgacct
     8941 gtctcagctg ggaggcgaca gccccaagaa gaagagaaag gtggaggcca gcggaggcgg
     9001 cggtagcgga ggaggcgggt ccggcggcgg cggtagtggg ccggccgaac tgcccacctg
     9061 cagctgtctt gatcgagtta tacaaaaaga caaaggccca tattatacac accttggggc
     9121 aggaccaagt gttgctgctg tcagggaaat catggagaat aggtatggtc aaaaaggaaa
     9181 cgcaataagg atagaaatag tagtgtacac cggtaaagaa gggaaaagct ctcatgggtg
     9241 tccaattgct aagtgggttt taagaagaag cagtgatgaa gaaaaagttc tttgtttggt
     9301 ccggcagcgt acaggccacc actgtccaac tgctgtgatg gtggtgctca tcatggtgtg
     9361 ggatggcatc cctcttccaa tggccgaccg gctatacaca gagctcacag agaatctaaa
     9421 gtcatacaat gggcacccta ccgacagaag atgcaccctc aatgaaaatc gtacctgtac
     9481 atgtcaagga attgatccag agacttgtgg agcttcattc tcttttggct gttcatggag
     9541 tatgtacttt aatggctgta agtttggtag aagcccaagc cccagaagat ttagaattga
     9601 tccaagctct cccttacatg aaaaaaacct tgaagataac ttacagagtt tggctacacg
     9661 attagctcca atttataagc agtatgctcc agtagcttac caaaatcagg tggaatatga
     9721 aaatgttgcc cgagaatgtc ggcttggcag caaggaaggt cgtcccttct ctggggtcac
     9781 tgcttgcctg gacttctgtg ctcatcccca cagggacatt cacaacatga ataatggaag
     9841 cactgtggtt tgtaccttaa ctcgagaaga taaccgctct ttgggtgtta ttcctcaaga
     9901 tgagcagctc catgtgctac ctctttataa gctttcagac acagatgagt ttggctccaa
     9961 ggaaggaatg gaagccaaga tcaaatctgg ggccatcgag gtcctggcac cccgccgcaa
    10021 aaaaagaacg tgtttcactc agcctgttcc ccgttctgga aagaagaggg ctgcgatgat
    10081 gacagaggtt cttgcacata agataagggc agtggaaaag aaacctattc cccgaatcaa
    10141 gcggaagaat aactcaacaa caacaaacaa cagtaagcct tcgtcactgc caaccttagg
    10201 gagtaacact gagaccgtgc aacctgaagt aaaaagtgaa accgaacccc attttatctt
    10261 aaaaagttca gacaacacta aaacttattc gctgatgcca tccgctcctc acccagtgaa
    10321 agaggcatct ccaggcttct cctggtcccc gaagactgct tcagccacac cagctccact
    10381 gaagaatgac gcaacagcct catgcgggtt ttcagaaaga agcagcactc cccactgtac
    10441 gatgccttcg ggaagactca gtggtgccaa tgcagctgct gctgatggcc ctggcatttc
    10501 acagcttggc gaagtggctc ctctccccac cctgtctgct cctgtgatgg agcccctcat
    10561 taattctgag ccttccactg gtgtgactga gccgctaacg cctcatcagc caaaccacca
    10621 gccctccttc ctcacctctc ctcaagacct tgcctcttct ccaatggaag aagatgagca
    10681 gcattctgaa gcagatgagc ctccatcaga cgaaccccta tctgatgacc ccctgtcacc
    10741 tgctgaggag aaattgcccc acattgatga gtattggtca gacagtgagc acatcttttt
    10801 ggatgcaaat attggtgggg tggccatcgc acctgctcac ggctcggttt tgattgagtg
    10861 tgcccggcga gagctgcacg ctaccactcc tgttgagcac cccaaccgta atcatccaac
    10921 ccgcctctcc cttgtctttt accagcacaa aaacctaaat aagccccaac atggttttga
    10981 actaaacaag attaagtttg aggctaaaga agctaagaat aagaaaatga aggcctcaga
    11041 gcaaaaagac caggcagcta atgaaggtcc agaacagtcc tctgaagtaa atgaattgaa
    11101 ccaaattcct tctcataaag cattaacatt aacccatgac aatgttgtca ccgtgtcccc
    11161 ttatgctctc acacacgttg cggggcccta taaccattgg gtcatcgatt gattaattaa
    11221 gaattcgacc cagctttctt gtacaaagtg gttggtaagc ctatccctaa ccctctcctc
    11281 ggtctcgatt ctacgtagta atgagctagc gctaaccggt ggcgcgttaa gtcgacaatc
    11341 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
    11401 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
    11461 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc
    11521 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt
    11581 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg
    11641 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
    11701 gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct
    11761 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc
    11821 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc
    11881 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcgtcga ctttaagacc
    11941 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga
    12001 agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg ggtctctctg
    12061 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
    12121 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
    12181 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtacgtatag
    12241 tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag
    12301 tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
    12361 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
    12421 tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccat cccgccccta
    12481 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
    12541 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
    12601 ggcctaggga cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc tcactggccg
    12661 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag
    12721 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
    12781 aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg
    12841 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
    12901 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
    12961 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
    13021 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
    13081 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
    13141 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
    13201 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta
    13261 caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
    13321 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
    13381 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
    13441 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
    13501 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
    13561 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
    13621 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
    13681 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
    13741 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
    13801 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
    13861 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
    13921 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
    13981 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
    14041 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
    14101 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
    14161 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
    14221 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
    14281 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
    14341 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
    14401 ccccgtagaa aagatcaaag gatcttc
//
