LOCUS       sequence-271459-        8477 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes the hAAVS1 BxBI recognition site with Hygromycin resistance 
            and mRuby2 landing pad with GFP dropout destination vector as a type
            0 part to be used in the MTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8477)
  AUTHORS   Fonseca JP, Bonny AR, Kumar GR, Ng AH, Town J, Wu QC, Aslankoohi E, 
            Chen SY, Dods G, Harrigan P, Osimiri LC, Kistler AL, El-Samad H
  TITLE     A Toolkit for Rapid Modular Construction of Biological Circuits in 
            Mammalian Cells.
  JOURNAL   ACS Synth Biol. 2019 Nov 15;8(11):2593-2606. doi: 
            10.1021/acssynbio.9b00322. Epub 2019 Nov 5.
  PUBMED    31686495
REFERENCE   2  (bases 1 to 8477)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.9b00322"; journalName: "ACS Synth Biol"; date: 
            "2019-11-15- 15"; volume: "8"; issue: "11"; pages: "2593-2606"
COMMENT     Sequence Label: MTK0_057
FEATURES             Location/Qualifiers
     source          1..8477
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    209..242
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     misc_feature    414..943
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     CDS             complement(1240..2055)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(1240..2055)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     1963..1982
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     2047..2066
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      2280..2868
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2769..2788
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    3374..3875
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     protein_bind    3938..3971
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     protein_bind    4589..4622
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     enhancer        4644..4947
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4949..5224
                     /label=chicken beta-actin promoter
     intron          5225..6241
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     6165..6187
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     6249..6268
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    6307..6354
                     /label=Bxb1 attP
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="Minimal attP site for the Mycopbacteriophage Bxb1 
                     integrase (Voutev and Mann, 2017)"
     CDS             6365..7384
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             6365..7384
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             7400..7453
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             7460..7480
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7496..8203
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="VSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTMR
                     IKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGGV
                     VTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTHM
                     ALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAVA
                     KFAGLGGGMDELYK"
     primer_bind     complement(8364..8383)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     8418..8437
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 gttacagaga tgttacgaac cctgaacagg ttgactatta attgtgtctg cttgatgtgg
       61 acaccaggtg gcgctggaca tcagatttgg agaggcagtt gtctagggaa ccgggctctg
      121 tgccagcgca ggaggcaggc tggctctcct attccaggga tgctcatcca ggaaggaaag
      181 gttgcatgct ggacacacta accttacaga agttcctatt ctctagaaag tataggaact
      241 tcgagtgatt ggtgacagaa aagccccatc cttaggcctc ctccttccta gtctcctgat
      301 attgggtcta acccccacct cctgttaggc agattcctta tctggtgaca cacccccatt
      361 tcctggagcc atctctctcc ttgccagaac ctctaaggtt tgcttacgat ggagccagag
      421 aggatcctgg gagggagagc ttggcagggg gtgggaggga agggggggat gcgtgacctg
      481 cccggttctc agtggccacc ctgcgctacc ctctcccaga acctgagctg ctctgacgcg
      541 gccgtctggt gcgtttcact gatcctggtg ctgcagcttc cttacacttc ccaagaggag
      601 aagcagtttg gaaaaacaaa atcagaataa gttggtcctg agttctaact ttggctcttc
      661 acctttctag tccccaattt atattgttcc tccgtgcgtc agttttacct gtgagataag
      721 gccagtagcc agccccgtcc tggcagggct gtggtgagga ggggggtgtc cgtgtggaaa
      781 actccctttg tgagaatggt gcgtcctagg tgttcaccag gtcgtggccg cctctactcc
      841 ctttctcttt ctccatcctt ctttccttaa agagtcccca gtgctatctg ggacatattc
      901 ctccgcccag agcagggtcc cgcttcccta aggccctgct ctgggcttct gggtttgagt
      961 ccttggcaag cccaggagag gcgctcaggc ttccctgtcc cccttcctcg tccaccatct
     1021 catgcccctg gctctcctgc cccttcccta caggggttcc tggctctgct ctacctactg
     1081 cattaaaaaa aaattttttt ttttctaaga caaggtctga ctctgtctcc caggctggag
     1141 tgcagtggtg caatcacgct catagcctca acctcccggg ctccagtggg cctcccacct
     1201 ccgagcggcc gcgtgttaca accaattaac caattctgat tagaaaaact catcgagcat
     1261 caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg
     1321 tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta
     1381 tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa
     1441 aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa
     1501 aagcttatgc atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa
     1561 atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga ggcgaaatac
     1621 gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac
     1681 tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc
     1741 tgttttcccg gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg
     1801 cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt
     1861 aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt
     1921 cccatacaat cgatagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata
     1981 cccatataaa tcagcatcca tgttggaatt taatcgcggc ctggagcaag acgtttcccg
     2041 ttgaatatgg ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt
     2101 tcatgatgat atatttttat cttgtgcaat gtaacatcag agattttgag acacaacgtg
     2161 gctttgttga ataaatcgaa cttttgctga gttgaaggat cagtcatgac caaaatccct
     2221 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     2281 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     2341 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     2401 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     2461 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     2521 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     2581 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     2641 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     2701 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     2761 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     2821 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     2881 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     2941 ttatcccctg attctgtgga taaccgtgcg gccgccaatc cacaaccagt aagggctggc
     3001 ccaggtgtct ggccccacag ctgagctcct aaccgcaccg agcctctcca aaacatggtc
     3061 tttttttgct ccccactgcc accaccatca ccgtcccctg cctcatccag tcccccttcc
     3121 cattctccag agacttcctg ggccatctcc atgaccttcc agaccagcct gggcttcact
     3181 ctcaaatcca atgctttctc tgacctgcat tctctcccct gggcctgtgc cgctttctgt
     3241 ctgcagcttg tggcctgggt cacctctacg gctggcccag atccttccct gccgcctcct
     3301 tcaggttccg tcttcctcca ctccctcttc cccttgctct ctgctgtgtt gctgcccaag
     3361 gatgctcttt ccggagcact tccttctcgg cgctgcacca cgtgatgtcc tctgagcgga
     3421 tcctccccgt gtctgggtcc tctccgggca tctctcctcc ctcacccaac cccatgccgt
     3481 cttcactcgc tgggttccct tttccttctc cttctggggc ctgtgccatc tctcgtttct
     3541 taggatggcc ttctccgacg gatgtctccc ttgcgtcccg cctccccttc ttgtaggcct
     3601 gcatcatcac cgtttttctg gacaacccca aagtaccccg tctgcctggc tttagccacc
     3661 tctccatcct cttgctttct ttgcctggac accccgttct cctgtggatt cgggtcacct
     3721 ctcactcctt tcatttgggc agctccccta ccccccttac ctctctagtc tgtgctagct
     3781 cttccagccc cctgtcatgg catcttccag gggtccgaga gctcagctag tcttcttcct
     3841 ccaacccggg cccctatgtc cacttcagga cagcatgttt gctgcctcca gggatcctgt
     3901 gtccccgagc tgggaccacc ttatattccc aggccctgaa gttcctattc tctagaaagt
     3961 ataggaactt cctgaactgg ccgataattg cagacgatcg aattcgagtt ggcgcgcctg
     4021 tcattctaaa tctctctttc agcctaaagc tttttccccg tatcccccca ggtgtctgca
     4081 ggctcaaaga gcagcgagaa gcgttcagag gaaagcgatc ccgtgccacc ttccccgtgc
     4141 ccgggctgtc cccgcacgct gccggctcgg ggatgcgggg ggagcgccgg accggagcgg
     4201 agccccgggc ggctcgctgc tgccccctag cgggggaggg acgtaattac atccctgggg
     4261 gctttgggag ggggctgtcc ccgtgagctc ccaggcgcgc ctgtcattct aaatctctct
     4321 ttcagcctaa agctttttcc ccgtatcccc ccaggtgtct gcaggctcaa agagcagcga
     4381 gaagcgttca gaggaaagcg atcccgtgcc accttccccg tgcccgggct gtccccgcac
     4441 gctgccggct cggggatgcg gggggagcgc cggaccggag cggagccccg ggcggctcgc
     4501 tgctgccccc tagcggggga gggacgtaat tacatccctg ggggctttgg gggggggctg
     4561 tccccgtgag ctcaataaaa gagcccacat aacttcgtat aatgtatgct atacgaagtt
     4621 ataacgccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
     4681 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
     4741 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
     4801 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
     4861 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
     4921 tacgtattag tcatcgctat taccatggtc gaggtgagcc ccacgttctg cttcactctc
     4981 cccatctccc ccccctcccc acccccaatt ttgtatttat ttatttttta attattttgt
     5041 gcagcgatgg gggcgggggg ggggggggsg cgcgccaggc ggggcggggc ggggcgaggg
     5101 gcggggcggg gcgaggcgga gaggtgcggc ggcagccaat cagagcggcg cgctccgaaa
     5161 gtttcctttt atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg
     5221 ggcgggagtc gctgcgcgct gccttcgccc cgtgccccgc tccgccgccg cctcgcgccg
     5281 cccgccccgg ctctgactga ccgcgttact cccacaggtg agcgggcggg acggcccttc
     5341 tcctccgggc tgtaattagc gcttggttta atgacggctt gtttcttttc tgtggctgcg
     5401 tgaaagcctt gaggggctcc gggagggccc tttgtgcggg gggagcggct cggggggtgc
     5461 gtgcgtgtgt gtgtgcgtgg ggagcgccgc gtgcggctcc gcgctgcccg gcggctgtga
     5521 gcgctgcggg cgcggcgcgg ggctttgtgc gctccgcagt gtgcgcgagg ggagcgcggc
     5581 cgggggcggt gccccgcggt gcgggggggg ctgcgagggg aacaaaggct gcgtgcgggg
     5641 tgtgtgcgtg ggggggtgag cagggggtgt gggcgcgtcg gtcgggctgc aaccccccct
     5701 gcacccccct ccccgagttg ctgagcacgg cccggcttcg ggtgcggggc tccgtacggg
     5761 gcgtggcgcg gggctcgccg tgccgggcgg ggggtggcgg caggtggggg tgccgggcgg
     5821 ggcggggccg cctcgggccg gggagggctc gggggagggg cgcggcggcc cccggagtgc
     5881 cggcggctgt cgaggcgcgg cgagccgcag ccattgcctt ttatggtaat cgtgcgagag
     5941 ggcgcaggga cttcctttgt cccaaatctg tgcggagccg aaatctggga ggcgccgccg
     6001 caccccctct agcgggcgcg gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg
     6061 ggagggcctt cgtgcgtcgc cgcgccgccg tccccttctc cctctccagc ctcggggctg
     6121 tccgcggggg gacggctgcc ttcggggggg acggggcagg gcggggttcg gcttctggcg
     6181 tgtgaccggc ggctctagag cctctgctaa ccatgttcat gccttcttct ttttcctaca
     6241 gctcctgggc aacgtgctgg ttattgtgct gtctcatcat tttggcaaag aattcgccac
     6301 tatggtggtt tgtctggtca accaccgcgg tctcagtggt gtacggtaca aaccaggttc
     6361 tggtaaaaag cctgaactca ccgcgacgtc tgtcgagaag tttctgatcg aaaagttcga
     6421 cagcgtgtcc gacctgatgc agctctcgga gggcgaagaa tctcgtgctt tcagcttcga
     6481 tgtaggaggg cgtggatatg tcctgcgggt aaatagctgc gccgatggtt tctacaaaga
     6541 tcgttatgtt tatcggcact ttgcatcggc cgcgctcccg attccggaag tgcttgacat
     6601 tggggaattt agcgagagcc tgacctattg catctcccgc cgtgcacagg gtgtcacgtt
     6661 gcaagacctg cctgaaaccg aactgcccgc tgttctgcaa ccggtcgcgg aggccatgga
     6721 tgcgatcgct gcggccgatc ttagccagac gagcgggttc ggcccattcg gaccgcaagg
     6781 aatcggtcaa tacactacat ggcgtgattt catatgcgcg attgctgatc cccatgtgta
     6841 tcactggcaa actgtgatgg acgacaccgt cagtgcgtcc gtcgcgcagg ctctcgatga
     6901 gctgatgctt tgggccgagg actgccccga agtccggcac ctcgtgcacg cggatttcgg
     6961 ctccaacaat gtcctgacgg acaatggccg cataacagcg gtcattgact ggagcgaggc
     7021 gatgttcggg gattcccaat acgaggtcgc caacatcttc ttctggaggc cgtggttggc
     7081 ttgtatggag cagcagacgc gctacttcga gcggaggcat ccggagcttg caggatcgcc
     7141 gcggctccgg gcgtatatgc tccgcattgg tcttgaccaa ctctatcaga gcttggttga
     7201 cggcaatttc gatgatgcag cttgggcgca gggtcgatgc gacgcaatcg tccgatccgg
     7261 agccgggact gtcgggcgta cacaaatcgc ccgcagaagc gcggccgtct ggaccgatgg
     7321 ctgtgtagaa gtactcgccg atagtggaaa ccgacgcccc agcactcgtc cgagggcaaa
     7381 ggaaggatcc ggaagcggag agggcagagg aagtctgcta acatgcggtg acgtcgagga
     7441 gaatcctgga cctggatccc ccaagaagaa gaggaaggtg gaggacgccg gatccgtgtc
     7501 caaaggagag gagttaatca aggaaaacat gagaatgaaa gttgtcatgg agggctccgt
     7561 taatggtcac caattcaagt gtacagggga aggtgaaggt aatccttaca tgggtacaca
     7621 aactatgaga attaaagtaa ttgaaggcgg accactacca tttgcatttg acattctggc
     7681 aacgtcattc atgtacggat cacgaacttt catcaagtac cctaaaggta taccagactt
     7741 tttcaagcaa tcttttccag agggttttac atgggaaagg gttacaagat acgaagatgg
     7801 gggtgtcgtc acagttatgc aagatacttc attagaagat ggctgccttg tctatcatgt
     7861 gcaagtaaga ggggtgaatt ttccttctaa cggacctgtg atgcagaaaa agaccaaagg
     7921 ttgggaacca aatactgaaa tgatgtaccc agctgatgga ggtttgagag gctacacaca
     7981 catggcgctt aaagttgatg gtggaggtca tttgtcttgt agttttgtta ccacttatcg
     8041 ttctaaaaag actgttggca atatcaaaat gccaggaata catgctgtag accacagact
     8101 agaaagactc gaagagagcg ataacgaaat gttcgttgta cagagagagc atgccgtagc
     8161 caaatttgct ggcttaggcg gtggtatgga tgaattgtat aagggatccc atggcttccc
     8221 gccggaggtg gaggagcagg atgatggcgc gctgcccatg tcttgtgccc aggagagcgg
     8281 gatggaccgt caccctgcag cctgtgcttc tgctaggatc aatgtgtaac tcgagtggcg
     8341 atccagacat gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa
     8401 aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct
     8461 gcaataaacg ctgagca
//
