LOCUS       sequence-271466-        6763 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Retroviral plasmid expressing GFP - Nde1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6763)
  AUTHORS   Monda JK, Cheeseman IM
  TITLE     Nde1 promotes diverse dynein functions through differential 
            interactions and exhibits an isoform-specific proteasome 
            association.
  JOURNAL   Mol Biol Cell. 2018 Sep 15;29(19):2336-2345. doi: 
            10.1091/mbc.E18-07-0418. Epub 2018 Jul 19.
  PUBMED    30024347
REFERENCE   2  (bases 1 to 6763)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E18-07-0418"; journalName: "Mol Biol Cell"; date: 
            "2018-09-15- 15"; volume: "29"; issue: "19"; pages: "2336-2345"
COMMENT     Sequence Label: pJM131
FEATURES             Location/Qualifiers
     source          1..6763
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             95..511
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     441..463
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     479..495
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     regulatory      527..536
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1219..1230
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             1553..1570
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1580..1603
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             1604..1627
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             join(1637..1639,1640..1642,1643..2356)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTATGITLGMDELYK"
     primer_bind     complement(1682..1703)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1943..1962)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2290..2311
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(2501..2521)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2506..2835
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2686..2821
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2843..3241
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             3410..3879
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(4009..4026)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4180..4768)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4260..4279)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4928..5719,5720..5788))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5551..5570
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5789..5893)
                     /gene="bla"
                     /label=AmpR promoter
     LTR             5924..6393
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    6458..6657
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
ORIGIN
        1 gaaccgaagc cgcgcgtctt gtctgctgca gcgctgcagc atcgttctgt gttgtctctg
       61 tctgactgtg tttctgtatt tgtctgaaaa ttagggccag actgttacca ctcccttaag
      121 tttgacctta ggtcactgga aagatgtcga gcggatcgct cacaaccagt cggtagatgt
      181 caagaagaga cgttgggtta ccttctgctc tgcagaatgg ccaaccttta acgtcggatg
      241 gccgcgagac ggcaccttta accgagacct catcacccag gttaagatca aggtcttttc
      301 acctggcccg catggacacc cagaccaggt cccctacatc gtgacctggg aagccttggc
      361 ttttgacccc cctccctggg tcaagccctt tgtacaccct aagcctccgc ctcctcttcc
      421 tccatccgcc ccgtctctcc cccttgaacc tcctcgttcg accccgcctc gatcctccct
      481 ttatccagcc ctcactcctt ctctaggcgc cggccggatc ctcgaggcca ccatggagga
      541 ctccggaaag actttcagct ccgaggagga agaagctaac tattggaaag atctggcgat
      601 gacctacaaa cagagggcag aaaatacgca agaggaactc cgagaattcc aggagggaag
      661 ccgagaatat gaagctgaat tggagacgca gctgcaacaa attgaaacca ggaacagaga
      721 cctcctgtcc gaaaataacc gccttcgcat ggagctggaa accatcaagg agaagtttga
      781 agtgcagcac tctgaaggct accggcagat ctcagccttg gaggatgacc tcgcgcagac
      841 caaagccatt aaagaccaat tgcagaaata catcagagag ctggagcaag caaatgacga
      901 cctggaaaga gccaagcgcg ccacgatcat gtctctcgaa gactttgagc agcgcttgaa
      961 tcaggccatc gaaagaaatg ccttcctgga aagtgaactt gatgaaaaag agaatctcct
     1021 ggaatctgtt cagagactga aggatgaagc cagagatttg cggcaggaac tggccgtgca
     1081 gcagaagcag gagaaaccca ggacccccat gcccagctca gtggaagctg agaggacaga
     1141 cacagctgtg caggccacgg gctccgtgcc gtccacgccc attgctcacc gaggaccaag
     1201 ctcaagttta aacacacctg ggagcttcag acgtggcctg gacgactcca ccggggggac
     1261 ccccctcaca cctgcggccc ggatatcagc cctcaacatt gtgggagacc tactgcggaa
     1321 agtcggggca ctggagtcca aactcgcttc ctgccggaac ctcgtgtacg atcagtcccc
     1381 aaaccgaaca ggtggcccag cctctgggcg gagcagcaag aacagagatg gcggggagag
     1441 acggccaagc agcaccagcg tgcctttggg tgataagggg ttggacacga gttgccgctg
     1501 gttgtccaaa tcaacaacca ggtcgtccag ctcctgcggc cgcggcagca gccatcatca
     1561 tcatcatcac agcagcggcc tggaagttct gttccagggg cccctggaag ttctgttcca
     1621 ggggccctcc ggagggatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat
     1681 cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga
     1741 gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc
     1801 cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta
     1861 ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca
     1921 ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt
     1981 cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg
     2041 caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc
     2101 cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg
     2161 cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct
     2221 gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa
     2281 gcgcgatcac atggtcctgc tggagttcgt gaccgccacc gggatcactc ttggcatgga
     2341 cgagctgtac aagtaaagat ccaccggatc tagataactg atcataatca gccataccac
     2401 atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca
     2461 taaaatgaat gcaattcgcc agcacagtgg tcgaccctgt ggaatgtgtg tcagttaggg
     2521 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     2581 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     2641 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
     2701 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     2761 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     2821 ctaggctttt gcaaaaagct tcatggccaa gcctttgtct caagaagaat ccaccctcat
     2881 tgaaagagca acggctacaa tcaacagcat ccccatctct gaagactaca gcgtcgccag
     2941 cgcagctctc tctagcgacg gccgcatctt cactggtgtc aatgtatatc attttactgg
     3001 gggaccttgt gcagaactcg tggtgctggg cactgctgct gctgcggcag ctggcaacct
     3061 gacttgtatc gtcgcgatcg gaaatgagaa caggggcatc ttgagcccct gcggacggtg
     3121 ccgacaggtg cttctcgatc tgcatcctgg gatcaaagcc atagtgaagg acagtgatgg
     3181 acagccgacg gcagttggga ttcgtgaatt gctgccctct ggttatgtgt gggagggcta
     3241 agatcgataa aataaaagat tttatttagt ctccagaaaa aggggggaat gaaagacccc
     3301 acctgtaggt ttggcaagct agcttaagta acgccatttt gcaaggcatg gaaaaataca
     3361 taactgagaa tagagaagtt cagatcaagg tcaggaacag atggaacagc tgaatatggg
     3421 ccaaacagga tatctgtggt aagcagttcc tgccccggct cagggccaag aacagatgga
     3481 acagctgaat atgggccaaa caggatatct gtggtaagca gttcctgccc cggctcaggg
     3541 ccaagaacag atggtcccca gatgcggtcc agccctcagc agtttctaga gaaccatcag
     3601 atgtttccag ggtgccccaa ggacctgaaa tgaccctgtg ccttatttga actaaccaat
     3661 cagttcgctt ctcgcttctg ttcgcgcgct tctgctcccc gagctcaata aaagagccca
     3721 caacccctca ctcggggcgc cagtcctccg attgactgag tcgcccgggt acccgtgtat
     3781 ccaataaacc ctcttgcagt tgcatccgac ttgtggtctc gctgttcctt gggagggtct
     3841 cctctgagtg attgactacc cgtcagcggg ggtctttcac atgcagcatg tatcaaaatt
     3901 aatttggttt tttttcttaa gtatttacat taaatggcca tagttgcatt aatgaatcgg
     3961 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     4021 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     4081 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     4141 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     4201 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     4261 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     4321 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     4381 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     4441 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     4501 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     4561 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     4621 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     4681 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     4741 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     4801 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     4861 cttcacctag atccttttgc ggccgcaaat caatctaaag tatatatgag taaacttggt
     4921 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     4981 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     5041 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     5101 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     5161 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     5221 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     5281 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     5341 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     5401 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     5461 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     5521 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     5581 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     5641 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     5701 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     5761 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     5821 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     5881 taggggttcc gcgcacattt ccccgaaaag tgccacctgc agcctgaata tgggccaaac
     5941 aggatatctg tggtaagcag ttcctgcccc ggctcagggc caagaacaga tggaacagct
     6001 gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga
     6061 acagatggtc cccagatgcg gtccagccct cagcagtttc tagagaacca tcagatgttt
     6121 ccagggtgcc ccaaggacct gaaatgaccc tgtgccttat ttgaactaac caatcagttc
     6181 gcttctcgct tctgttcgcg cgcttctgct ccccgagctc aataaaagag cccacaaccc
     6241 ctcactcggg gcgccagtcc tccgattgac tgagtcgccc gggtacccgt gtatccaata
     6301 aaccctcttg cagttgcatc cgacttgtgg tctcgctgtt ccttgggagg gtctcctctg
     6361 agtgattgac tacccgtcag cgggggtctt tcatttgggg gctcgtccgg gatcgggaga
     6421 cccctgccca gggaccaccg acccaccacc gggaggcaag ctggccagca acttatctgt
     6481 gtctgtccga ttgtctagtg tctatgactg attttatgcg cctgcgtcgg tactagttag
     6541 ctaactagct ctgtatctgg cggacccgtg gtggaactga cgagttctga acacccggcc
     6601 gcaaccctgg gagacgtccc agggactttg ggggccgttt ttgtggcccg acctgaggaa
     6661 gggagtcgat gtggaatccg accccgtcag gatatgtggt tctggtagga gacgagaacc
     6721 taaaacagtt cccgcctccg tctgaatttt tgctttcggt ttg
//
