LOCUS       sequence-271470-       12819 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  3rd generation lentiviral expression of TALE-hTET1(CD) in mammalian 
            cells targeting MLH1 promoter region.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12819)
  AUTHORS   Taghbalout A, Du M, Jillette N, Rosikiewicz W, Rath A, Heinen CD, Li
            S, Cheng AW
  TITLE     Enhanced CRISPR-based DNA demethylation by Casilio-ME-mediated 
            RNA-guided coupling of methylcytosine oxidation and DNA repair 
            pathways.
  JOURNAL   Nat Commun. 2019 Sep 20;10(1):4296. doi: 10.1038/s41467-019-12339-7.
  PUBMED    31541098
REFERENCE   2  (bases 1 to 12819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-09-20"; volume: "10(1):4296. doi"; pages: " 
            10.1038/s41467-019-12339-7"
COMMENT     Sequence Label: pAT801_CMV/CAG_TALE_A-hTET1(CD)
FEATURES             Location/Qualifiers
     source          1..12819
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1010..1030
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1012..1030
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1058..1284
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1285..1465
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1512..1637
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2130..2363
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2548..2592
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2767..3267
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     3186..3204
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             3289..3888
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3289..3308)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3785..3805
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    3930..4047
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        4164..4467
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4468..4671
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4622..4642
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    4694..4718
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4745..4762
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             4787..4807
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4811..4831
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4835..4855
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7331..7351
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7355..7375
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    complement(9620..9644)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             9646..9687
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    9729..10317
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9782..9802)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10200..10211)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             10389..10622
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    10700..10821
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10737..10756)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10791..10810
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      10861..10996
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(11016..11035)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(11017..11035)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(11045..11062)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(11045..11061)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11054..11076)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      11203..11658
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11290..11309)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11500..11521
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        11684..11788
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11789..11857,11858..12649)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(12007..12026)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     1021 tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc aatactcttg
     1081 tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac
     1141 cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag
     1201 acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga tattgtattt
     1261 aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg agcctgggag
     1321 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     1381 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     1441 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga
     1501 aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg
     1561 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag
     1621 atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc
     1681 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg
     1741 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa
     1801 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata
     1861 atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag
     1921 ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg
     1981 ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa
     2041 tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg
     2101 gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca
     2161 gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg
     2221 tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg
     2281 ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga
     2341 tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc
     2401 actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac
     2461 acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta
     2521 attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa
     2581 tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc
     2641 ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg
     2701 aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg
     2761 ggacccttgc gccttttcca aggcagccct gggtttgcgc agggacgcgg ctgctctggg
     2821 cgtggttccg ggaaacgcag cggcgccgac cctgggtctc gcacattctt cacgtccgtt
     2881 cgcagcgtca cccggatctt cgccgctacc cttgtgggcc ccccggcgac gcttcctgct
     2941 ccgcccctaa gtcgggaagg ttccttgcgg ttcgcggcgt gccggacgtg acaaacggaa
     3001 gccgcacgtc tcactagtac cctcgcagac ggacagcgcc agggagcaat ggcagcgcgc
     3061 cgaccgcgat gggctgtggc caatagcggc tgctcagcag ggcgcgccga gagcagcggc
     3121 cgggaagggg cggtgcggga ggcggggtgt ggggcggtag tgtgggccct gttcctgccc
     3181 gcgcggtgtt ccgcattctg caagcctccg gagcgcacgt cggcagtcgg ctccctcgtt
     3241 gaccgaatca ccgacctctc tccccagggg gtaccaccgg agcttaccat gaccgagtac
     3301 aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc
     3361 gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag
     3421 cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg
     3481 tgggtcgcgg acgacggcgc cgccgtggcg gtctggacca cgccggagag cgtcgaagcg
     3541 ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc
     3601 gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc
     3661 ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg
     3721 ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg
     3781 ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg
     3841 cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgaga attctagatc
     3901 ttgagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat tggggggtac
     3961 agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa
     4021 aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag agatccactt
     4081 tggcgccggc tcgagggggc ccggggttat taatagtaat caattacggg gtcattagtt
     4141 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
     4201 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
     4261 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
     4321 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
     4381 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
     4441 tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt
     4501 ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt
     4561 ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg
     4621 acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc tctctggcta
     4681 actgtcggga tcaacaagtt tgtacaaaaa agcaggctcc gaattcaccg gtgccgccac
     4741 catgcaccac catcatcacc atatcgatgg cggaggcgga tctgatccaa aaaagaagag
     4801 aaaggtagat ccaaaaaaga agagaaaggt agatccaaaa aagaagagaa aggtaggatc
     4861 taccggatct agaaacgatg gtggtggtgg aagcgggggt gggggcagcg gtggaggggg
     4921 aagcgggcgc gccgtggatc tacgcacgct cggctacagt cagcagcagc aagagaagat
     4981 caaaccgaag gtgcgttcga cagtggcgca gcaccacgag gcactggtgg gccatgggtt
     5041 tacacacgcg cacatcgttg cgctcagcca acacccggca gcgttaggga ccgtcgctgt
     5101 cacgtatcag cacataatca cggcgttgcc agaggcgaca cacgaagaca tcgttggcgt
     5161 cggcaaacag tggtccggcg cacgcgccct ggaggccttg ctcacggatg cgggggagtt
     5221 gagaggtccg ccgttacagt tggacacagg ccaacttgtg aagattgcaa aacgtggcgg
     5281 cgtgaccgca atggaggcag tgcatgcatc gcgcaatgca ctgacgggtg cccccctgaa
     5341 cctgaccccg gaccaagtgg tggctatcgc cagcaacaat ggcggcaagc aagcgctcga
     5401 aacggtgcag cggctgttgc cggtgctgtg ccaggaccat ggcctgaccc cggaccaagt
     5461 ggtggctatc gccagcaaca ttggcggcaa gcaagcgctc gaaacggtgc agcggctgtt
     5521 gccggtgctg tgccaggacc atggcctgac cccggaccaa gtggtggcta tcgccagcaa
     5581 caatggcggc aagcaagcgc tcgaaacggt gcagcggctg ttgccggtgc tgtgccagga
     5641 ccatggcctg accccggacc aagtggtggc tatcgccagc aacattggcg gcaagcaagc
     5701 gctcgaaacg gtgcagcggc tgttgccggt gctgtgccag gaccatggcc tgaccccgga
     5761 ccaagtggtg gctatcgcca gcaacattgg cggcaagcaa gcgctcgaaa cggtgcagcg
     5821 gctgttgccg gtgctgtgcc aggaccatgg cctgaccccg gaccaagtgg tggctatcgc
     5881 cagcaacatt ggcggcaagc aagcgctcga aacggtgcag cggctgttgc cggtgctgtg
     5941 ccaggaccat ggcctgaccc cggaccaagt ggtggctatc gccagcaacg gtggcggcaa
     6001 gcaagcgctc gaaacggtgc agcggctgtt gccggtgctg tgccaggacc atggcctgac
     6061 cccggaccaa gtggtggcta tcgccagcaa cggtggcggc aagcaagcgc tcgaaacggt
     6121 gcagcggctg ttgccggtgc tgtgccagga ccatggcctg accccggacc aagtggtggc
     6181 tatcgccagc aacggtggcg gcaagcaagc gctcgaaacg gtgcagcggc tgttgccggt
     6241 gctgtgccag gaccatggcc tgaccccgga ccaagtggtg gctatcgcca gcaacaatgg
     6301 cggcaagcaa gcgctcgaaa cggtgcagcg gctgttgccg gtgctgtgcc aggaccatgg
     6361 cctgaccccg gaccaagtgg tggctatcgc cagcaacatt ggcggcaagc aagcgctcga
     6421 aacggtgcag cggctgttgc cggtgctgtg ccaggaccat ggcctgactc cggaccaagt
     6481 ggtggctatc gccagccacg atggcggcaa gcaagcgctc gaaacggtgc agcggctgtt
     6541 gccggtgctg tgccaggacc atggcctgac cccggaccaa gtggtggcta tcgccagcaa
     6601 cggtggcggc aagcaagcgc tcgaaacggt gcagcggctg ttgccggtgc tgtgccagga
     6661 ccatggcctg accccggacc aagtggtggc tatcgccagc aacaatggcg gcaagcaagc
     6721 gctcgaaacg gtgcagcggc tgttgccggt gctgtgccag gaccatggcc tgaccccgga
     6781 ccaagtggtg gctatcgcca gcaacaatgg cggcaagcaa gcgctcgaaa cggtgcagcg
     6841 gctgttgccg gtgctgtgcc aggaccatgg cctgactccg gaccaagtgg tggctatcgc
     6901 cagccacgat ggcggcaagc aagcgctcga aacggtgcag cggctgttgc cggtgctgtg
     6961 ccaggaccat ggcctgaccc cggaccaagt ggtggctatc gccagcaaca ttggcggcaa
     7021 gcaagcgctc gaaacggtgc agcggctgtt gccggtgctg tgccaggacc atggcctgac
     7081 cccggaccaa gtggtggcta tcgccagcaa cggtggcggc aagcaagcgc tcgaaagcat
     7141 tgtggcccag ctgagccggc ctgatccggc gttggccgcg ttgaccaacg accacctcgt
     7201 cgccttggcc tgcctcggcg gacgtcctgc catggatgca gtgaaaaagg gattgccgca
     7261 cgcgccggaa ttgatcagaa gagtcaatcg ccgtattggc gaacgcacgt cccatcgcgt
     7321 tgccagggac ccaaagaaga agcgcaaagt ggatcctaaa aagaaaagaa aggtaggcgg
     7381 ccgcggggga ggcggttccg gtggcggcgg aagcggaggt ggaggatcag ggccggccga
     7441 actgcccacc tgcagctgtc ttgatcgagt tatacaaaaa gacaaaggcc catattatac
     7501 acaccttggg gcaggaccaa gtgttgctgc tgtcagggaa atcatggaga ataggtatgg
     7561 tcaaaaagga aacgcaataa ggatagaaat agtagtgtac accggtaaag aagggaaaag
     7621 ctctcatggg tgtccaattg ctaagtgggt tttaagaaga agcagtgatg aagaaaaagt
     7681 tctttgtttg gtccggcagc gtacaggcca ccactgtcca actgctgtga tggtggtgct
     7741 catcatggtg tgggatggca tccctcttcc aatggccgac cggctataca cagagctcac
     7801 agagaatcta aagtcataca atgggcaccc taccgacaga agatgcaccc tcaatgaaaa
     7861 tcgtacctgt acatgtcaag gaattgatcc agagacttgt ggagcttcat tctcttttgg
     7921 ctgttcatgg agtatgtact ttaatggctg taagtttggt agaagcccaa gccccagaag
     7981 atttagaatt gatccaagct ctcccttaca tgaaaaaaac cttgaagata acttacagag
     8041 tttggctaca cgattagctc caatttataa gcagtatgct ccagtagctt accaaaatca
     8101 ggtggaatat gaaaatgttg cccgagaatg tcggcttggc agcaaggaag gtcgtccctt
     8161 ctctggggtc actgcttgcc tggacttctg tgctcatccc cacagggaca ttcacaacat
     8221 gaataatgga agcactgtgg tttgtacctt aactcgagaa gataaccgct ctttgggtgt
     8281 tattcctcaa gatgagcagc tccatgtgct acctctttat aagctttcag acacagatga
     8341 gtttggctcc aaggaaggaa tggaagccaa gatcaaatct ggggccatcg aggtcctggc
     8401 accccgccgc aaaaaaagaa cgtgtttcac tcagcctgtt ccccgttctg gaaagaagag
     8461 ggctgcgatg atgacagagg ttcttgcaca taagataagg gcagtggaaa agaaacctat
     8521 tccccgaatc aagcggaaga ataactcaac aacaacaaac aacagtaagc cttcgtcact
     8581 gccaacctta gggagtaaca ctgagaccgt gcaacctgaa gtaaaaagtg aaaccgaacc
     8641 ccattttatc ttaaaaagtt cagacaacac taaaacttat tcgctgatgc catccgctcc
     8701 tcacccagtg aaagaggcat ctccaggctt ctcctggtcc ccgaagactg cttcagccac
     8761 accagctcca ctgaagaatg acgcaacagc ctcatgcggg ttttcagaaa gaagcagcac
     8821 tccccactgt acgatgcctt cgggaagact cagtggtgcc aatgcagctg ctgctgatgg
     8881 ccctggcatt tcacagcttg gcgaagtggc tcctctcccc accctgtctg ctcctgtgat
     8941 ggagcccctc attaattctg agccttccac tggtgtgact gagccgctaa cgcctcatca
     9001 gccaaaccac cagccctcct tcctcacctc tcctcaagac cttgcctctt ctccaatgga
     9061 agaagatgag cagcattctg aagcagatga gcctccatca gacgaacccc tatctgatga
     9121 ccccctgtca cctgctgagg agaaattgcc ccacattgat gagtattggt cagacagtga
     9181 gcacatcttt ttggatgcaa atattggtgg ggtggccatc gcacctgctc acggctcggt
     9241 tttgattgag tgtgcccggc gagagctgca cgctaccact cctgttgagc accccaaccg
     9301 taatcatcca acccgcctct cccttgtctt ttaccagcac aaaaacctaa ataagcccca
     9361 acatggtttt gaactaaaca agattaagtt tgaggctaaa gaagctaaga ataagaaaat
     9421 gaaggcctca gagcaaaaag accaggcagc taatgaaggt ccagaacagt cctctgaagt
     9481 aaatgaattg aaccaaattc cttctcataa agcattaaca ttaacccatg acaatgttgt
     9541 caccgtgtcc ccttatgctc tcacacacgt tgcggggccc tataaccatt gggtcatcga
     9601 ttgattaatt aagaattcga cccagctttc ttgtacaaag tggttggtaa gcctatccct
     9661 aaccctctcc tcggtctcga ttctacgtag taatgagcta gcgctaaccg gtggcgcgtt
     9721 aagtcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
     9781 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
     9841 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
     9901 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
     9961 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
    10021 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
    10081 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt
    10141 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
    10201 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc
    10261 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcgtc
    10321 gactttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa
    10381 ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac
    10441 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
    10501 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
    10561 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
    10621 cagtacgtat agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg
    10681 aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa
    10741 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
    10801 caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc
    10861 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
    10921 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
    10981 ggcttttttg gaggcctagg gacgtaccca attcgcccta tagtgagtcg tattacgcgc
    11041 gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta
    11101 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
    11161 atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg
    11221 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
    11281 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
    11341 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
    11401 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
    11461 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
    11521 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
    11581 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa
    11641 tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
    11701 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
    11761 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
    11821 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
    11881 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
    11941 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
    12001 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
    12061 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
    12121 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
    12181 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
    12241 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
    12301 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
    12361 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
    12421 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
    12481 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
    12541 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
    12601 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
    12661 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
    12721 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
    12781 ctgagcgtca gaccccgtag aaaagatcaa aggatcttc
//
