LOCUS       sequence-271495-        9634 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses EXOC7 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9634)
  AUTHORS   Wang S, Crisman L, Miller J, Datta I, Gulbranson DR, Tian Y, Yin Q, 
            Yu H, Shen J
  TITLE     Inducible Exoc7/Exo70 knockout reveals a critical role of the 
            exocyst in insulin-regulated GLUT4 exocytosis.
  JOURNAL   J Biol Chem. 2019 Dec 27;294(52):19988-19996. doi: 
            10.1074/jbc.RA119.010821. Epub 2019 Nov 18.
  PUBMED    31740584
REFERENCE   2  (bases 1 to 9634)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.RA119.010821"; journalName: "J Biol Chem"; date: 
            "2019-12-27- 27"; volume: "294"; issue: "52"; pages: "19988-19996"
COMMENT     Sequence Label: SHC003BSD-EXOC7
FEATURES             Location/Qualifiers
     source          1..9634
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     132..151
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     385..402
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    519..540
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(555..560,561..578,579..585)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    593..609
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     598..620
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     617..633
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     617..633
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     652..672
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        654..672
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        700..926
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             927..1107
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1154..1279
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1772..2005
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2190..2234
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2532..2780
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     2532..2552
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     2703..2722
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     enhancer        2843..3146
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3147..3350
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3301..3321
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      3382..3391
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3391..3456
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    5694..5811
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        5860..6370
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     6289..6307
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     LTR             6852..7085
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7157..7278
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7194..7213)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7248..7267
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7318..7453
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7473..7492)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7474..7492)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7502..7519)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7502..7518)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7511..7533)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7660..8115
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7747..7766)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7957..7978
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8141..8245
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8246..8314,8315..9106)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8464..8483)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(9277..9634,1..231)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
       61 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
      121 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
      181 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
      241 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
      301 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
      361 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
      421 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
      481 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat
      541 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc
      601 ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcaattaac
      661 cctcactaaa gggaacaaaa gctggagctg caagcttaat gtagtcttat gcaatactct
      721 tgtagtcttg caacatggta acgatgagtt agcaacatgc cttacaagga gagaaaaagc
      781 accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg tgccttatta ggaaggcaac
      841 agacgggtct gacatggatt ggacgaacca ctgaattgcc gcattgcaga gatattgtat
      901 ttaagtgcct agctcgatac ataaacgggt ctctctggtt agaccagatc tgagcctggg
      961 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
     1021 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
     1081 tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg
     1141 gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg
     1201 cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag
     1261 agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat
     1321 tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag
     1381 ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca
     1441 aatactggga cagctacaac catcccttca gacaggatca gaagaactta gatcattata
     1501 taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga
     1561 agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc
     1621 cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata
     1681 aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag
     1741 tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag
     1801 cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat
     1861 tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc
     1921 tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa
     1981 gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca
     2041 ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc
     2101 acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct
     2161 taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata
     2221 aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat
     2281 tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag
     2341 tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga
     2401 ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac agagacagat
     2461 ccattcgatt agtgaacgga tctcgacggt atcgatcacg agactagcct cgagcggccg
     2521 cccccttcac cgagggccta tttcccatga ttccttcata tttgcatata cgatacaagg
     2581 ctgttagaga gataattgga attaatttga ctgtaaacac aaagatatta gtacaaaata
     2641 cgtgacgtag aaagtaataa tttcttgggt agtttgcagt tttaaaatta tgttttaaaa
     2701 tggactatca tatgcttacc gtaacttgaa agtatttcga tttcttggct ttatatatct
     2761 tgtggaaagg acgaaacacc ggtagttatt aatagtaatc aattacgggg tcattagttc
     2821 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
     2881 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
     2941 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
     3001 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
     3061 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
     3121 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg
     3181 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
     3241 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga
     3301 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct ggtttagtga
     3361 accgtcagat ccgctagcct agccaccatg gactacaaag accatgacgg tgattataaa
     3421 gatcatgaca tcgattacaa ggatgacgat gacaagatga ttcccccaca ggaggcatcc
     3481 gctcgacggc gggagattga ggacaagctg aagcaggagg aggagactct gtccttcatc
     3541 cgagacagcc tggagaagag cgaccagctc actaagaaca tggtgtctat cttatcatcc
     3601 tttgagagcc gccttatgaa gctggagaac tccatcatcc ctgtgcacaa gcagacggag
     3661 aatctgcagc ggctgcagga gaatgttgag aagacgctgt cctgcctgga ccatgtcatc
     3721 agctactacc atgtggccag tgacactgag aagatcatca gagagggccc cacaggtagg
     3781 ctggaagagt acctgggaag catggccaag attcagaagg cagtggagta tttccaggac
     3841 aacagcccag acagcccgga actcaacaaa gtgaaactgc tctttgagcg cgggaaggag
     3901 gccctggagt ccgaatttcg cagcctgatg acgcggcaca gtaaggtcgt ctcgcccgtg
     3961 ctcatcttgg atctgatcag tggtgacgat gatctggagg cccaggagga cgtgaccctg
     4021 gagcacctgc ccgagagcgt gctccaggat gtcattcgca tctcccgctg gctggtggaa
     4081 tatggccgca accaagattt catgaacgtc tactaccaga tacgctccag ccagctggac
     4141 cgctccatca aaggactgaa ggagcatttc cataagagca gttcttcctc tggggttccc
     4201 tactcccctg ctatccccaa caagaggaaa gacacaccta ccaagaagcc agtcaagcgg
     4261 ccagggacga tccgtaaggc tcagaacctt ctgaaacagt attcccagca tggtctagat
     4321 gggaaaaagg ggggctctaa cctcattcct ctggaaggga gagatgacat gctggacgtg
     4381 gagaccgatg cctacatcca ctgcgtcagt gccttcgtca agctggcgca gagcgagtac
     4441 cagctgctgg ccgacatcat ccccgagcac caccagaaga agaccttcga ctccctgata
     4501 caggatgccc tggatgggct gatgcttgaa ggggagaaca tcgtgtctgc tgcccggaag
     4561 gccattgtgc gacacgactt ctccacggtg ctcaccgtct tccccatcct gcgacacctc
     4621 aagcagacca agcctgagtt tgaccaggtg ctccagggca cggctgccag cacaaagaac
     4681 aagctgcctg gcctcatcac atccatggag accatcggtg ccaaagcgct ggaggacttc
     4741 gcagacaaca tcaagaatga cccggacaag gagtacaaca tgccgaagga cggcaccgta
     4801 cacgagctca ccagcaatgc catcctcttc ctgcagcagc ttttggactt ccaggagacg
     4861 gcaggcgcca tgctggcctc ccaagagacc agctcttcgg ccaccagcta cagctctgag
     4921 ttcagcaagc ggctgctaag cacctatatc tgtaaagtgc tgggcaacct gcagttgaac
     4981 ttgctgagca agtccaaggt gtacgaggac ccagctctga gcgccatctt cctgcacaac
     5041 aactacaatt acatcctcaa gtccctggag aagtctgaac tgatccagct ggtggcagtg
     5101 acacagaaga ctgctgagcg ctcctaccgg gagcacattg agcagcagat ccagacctac
     5161 cagcgcagct ggttaaaggt gactgattac atcgcagaga agaatctacc tgtgttccag
     5221 ccgggagtca agctccggga caaggagcgg cagattatca aggagcgttt taagggcttc
     5281 aatgatggcc tcgaagaact gtgcaaaatc cagaaggcct gggctattcc agacacagag
     5341 cagagggaca ggattcgcca ggcccagaag accattgtca aggagaccta cggggccttt
     5401 ctacagaagt ttggcagcgt gcccttcacc aagaacccgg agaagtacat caagtacggg
     5461 gtggagcagg tgggcgacat gatcgatcgc cttttcgaca cctctgccta agtcgacccc
     5521 cagcatcctg cagaacgggg gccccatgtt cgccttccgc cgcgtggagg aggatcacag
     5581 caacaccgag ctgggcatcg tggagtacca gcacgccttc aagaccccgg atgcagatgc
     5641 cggtgaagaa taaaattctc gacctcgaga caaatggcag tattcatcca caattttaaa
     5701 agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca
     5761 gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat
     5821 tacagggaca gcagagatcc actttggccg cggctcgagg gggttggggt tgcgcctttt
     5881 ccaaggcagc cctgggtttg cgcagggacg cggctgctct gggcgtggtt ccgggaaacg
     5941 cagcggcgcc gaccctgggt ctcgcacatt cttcacgtcc gttcgcagcg tcacccggat
     6001 cttcgccgct acccttgtgg gccccccggc gacgcttcct gctccgcccc taagtcggga
     6061 aggttccttg cggttcgcgg cgtgccggac gtgacaaacg gaagccgcac gtctcactag
     6121 taccctcgca gacggacagc gccagggagc aatggcagcg cgccgaccgc gatgggctgt
     6181 ggccaatagc ggctgctcag cagggcgcgc cgagagcagc ggccgggaag gggcggtgcg
     6241 ggaggcgggg tgtggggcgg tagtgtgggc cctgttcctg cccgcgcggt gttccgcatt
     6301 ctgcaagcct ccggagcgca cgtcggcagt cggctccctc gttgaccgaa tcaccgacct
     6361 ctctccccag ggggatccct agccaccatg cctttgtctc aagaagaatc caccctcatt
     6421 gaaagagcaa cggctacaat caacagcatc cccatctctg aagactacag cgtcgccagc
     6481 gcagctctct ctagcgacgg ccgcatcttc actggtgtca atgtatatca ttttactggg
     6541 ggaccttgtg cagaactcgt ggtgctgggc actgctgctg ctgcggcagc tggcaacctg
     6601 acttgtatcg tcgcgatcgg aaatgagaac aggggcatct tgagcccctg cggacggtgc
     6661 cgacaggtgc ttctcgatct gcatcctggg atcaaagcca tagtgaagga cagtgatgga
     6721 cagccgacgg cagttgggat tcgtgaattg ctgccctctg gttatgtgtg ggagggctaa
     6781 ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga
     6841 aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg
     6901 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     6961 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     7021 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     7081 tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat
     7141 atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     7201 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     7261 actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccatc
     7321 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     7381 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
     7441 ttttttggag gcctagggac gtacccaatt cgccctatag tgagtcgtat tacgcgcgct
     7501 cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc
     7561 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc
     7621 gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt agcggcgcat
     7681 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     7741 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     7801 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     7861 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     7921 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     7981 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
     8041 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat
     8101 taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     8161 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     8221 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     8281 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     8341 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     8401 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     8461 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     8521 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     8581 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     8641 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     8701 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     8761 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     8821 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     8881 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     8941 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     9001 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     9061 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     9121 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     9181 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     9241 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     9301 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     9361 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     9421 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     9481 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     9541 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     9601 gggttcgtgc acacagccca gcttggagcg aacg
//
