LOCUS       sequence-271505-        9871 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CRISPRa Gateway entry clone for catalytically dead AaCas12b (D570A) 
            fused with TV activation system, followed by T2A and MCP-TV fusion 
            protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9871)
  AUTHORS   Ming M, Ren Q, Pan C, He Y, Zhang Y, Liu S, Zhong Z, Wang J, Malzahn
            AA, Wu J, Zheng X, Zhang Y, Qi Y
  TITLE     CRISPR-Cas12b enables efficient plant genome engineering.
  JOURNAL   Nat Plants. 2020 Mar;6(3):202-208. doi: 10.1038/s41477-020-0614-6. 
            Epub 2020 Mar 9.
  PUBMED    32170285
REFERENCE   2  (bases 1 to 9871)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41477-020-0614-6"; journalName: "Nat Plants"; date: 
            "2020-03"; volume: "6"; issue: "3"; pages: "202-208"
COMMENT     Sequence Label: pYPQ292 (AaCas12b)-D570A-TV-MS2-TV
FEATURES             Location/Qualifiers
     source          1..9871
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(1..87)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     136..158
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     150..167
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     151..167
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    183..282
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             307..327
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3736..3783
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             4867..5016
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             5029..5178
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             5179..5232
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5236..5622
                     /codon_start=1
                     /product="bacteriophage MS2 coat protein"
                     /label=MS2-N55K
                     /note="binds with high affinity to a specific stem-loop 
                     structure in the viral RNA (Lim et al., 1994)"
                     /translation="ASNFTQFVLVDNGGTGDVTVAPSNFANGVAEWISSNSRSQAYKVT
                     CSVRQSSAQKRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATNSDCELI
                     VKAMQGLLKDGNPIPSAIAANSGIY"
     CDS             5623..5643
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6715..6864
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             6877..7026
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     terminator      7043..7295
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    7340..7463
                     /gene="mutant version of attR"
                     /label=attR5
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(7480..7499)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7481..7499)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7504..7520)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7504..7520)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             7951..8742
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      8835..9423
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9324..9343
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9577..9594
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(9753..9780)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
ORIGIN
        1 atttgtccta ctcaggagag cgttcaccga caaacaacag ataaaacgaa aggcccagtc
       61 ttccgactga gcctttcgtt ttatttgatg cctggcagtt ccctactctc gcgttaacgc
      121 ttgcatggat gttttcccag tcacgacgtt gtaaaacgac ggccagtctt aagctcgggc
      181 cccaaataat gattttattt tgactgatag tgacctgttc gttgcaacaa attgatgagc
      241 aatgcttttt tataatgcca actttgtaca aaaaagcagg ctccgaattc gcccttcacc
      301 atggctccta agaagaagcg gaaggttggt attcacgggg tgcctgcggc tgccgtcaag
      361 tccatgaagg tcaagttgcg cctggataac atgccagaga tcagagccgg actttggaaa
      421 cttcacaccg aggttaatgc gggtgtgcgg tactatacgg aatggcttag ccttttgagg
      481 caagaaaatc tttatcggag gagtcccaat ggcgatggag aacaagaatg ctataaaact
      541 gctgaggaat gcaaggctga actccttgag agactcagag cccgccaagt tgagaatggg
      601 cactgcggcc ctgctgggag tgatgacgaa ctgctgcaat tggcacggca actttatgaa
      661 cttctggtcc cacaagcaat cggggctaaa ggtgatgcgc agcaaatcgc aaggaagttt
      721 cttagtcccc ttgccgacaa ggatgccgtg ggtggtttgg gaatagcaaa agcaggaaat
      781 aagcctaggt gggttcggat gagggaggct ggagagccag gttgggaaga ggaaaaggct
      841 aaagccgagg cgagaaagag tacggataga accgccgatg ttcttcgcgc tcttgcagac
      901 ttcggtctta aacctcttat gagagtctac acagactcag acatgtccag cgtgcagtgg
      961 aaaccacttc gcaaaggaca agcggtcaga acctgggata gagacatgtt ccaacaagcg
     1021 atcgaaagaa tgatgagttg ggaatcgtgg aatcagcgcg ttggagaagc gtacgcaaag
     1081 ctcgtggaac aaaagtcgag gtttgaacag aaaaattttg tgggacaaga acatcttgtc
     1141 caacttgtca atcaacttca acaagacatg aaggaagcat cacacggcct ggagtcgaaa
     1201 gaacaaactg cgcattactt gactgggaga gcgctgagag ggagcgacaa agtttttgag
     1261 aagtgggaaa aactcgatcc tgatgcccca tttgacctct atgataccga aatcaagaat
     1321 gttcaacgga ggaatactcg caggttcgga tctcatgatc tgtttgcgaa gctcgcggaa
     1381 cctaaatatc aggcgctctg gagagaggac gcttctttcc tcacgaggta tgcggtttac
     1441 aatagcattg tcagaaaact gaatcacgct aaaatgtttg cgacttttac tcttccggat
     1501 gctaccgccc acccgatctg gacgcggttt gacaaactcg gcggcaacct gcaccagtac
     1561 actttcttgt ttaacgaatt tggcgagggc aggcacgcca ttcggtttca gaagctgttg
     1621 acggttgagg atggcgttgc taaagaggtc gacgacgtca cggttccgat ttctatgtcc
     1681 gcgcagctgg atgacctctt gcctcgggac ccacacgagc tcgttgcact ctacttccag
     1741 gactacggtg cagaacaaca tctggctgga gagtttggcg gcgcgaaaat tcaataccgc
     1801 cgcgatcaat tgaaccacct gcacgccaga agaggcgcca gagatgtcta ccttaatctg
     1861 agcgtccgcg ttcagtcaca atccgaagcc aggggagaaa ggcgccctcc gtatgcagcg
     1921 gtcttcaggc ttgttggcga taaccaccgc gcgtttgttc actttgataa attgtcagat
     1981 tacctcgcag aacacccaga cgatggtaag ctggggtcgg aaggtttgct ctctgggctc
     2041 agagtcatgt cagttgcctt gggtcttagg acttccgcga gcatatctgt cttccgcgtc
     2101 gcaagaaagg acgaattgaa gccgaacagt gaaggccggg tccctttttg cttcccgatc
     2161 gaagggaacg aaaacctcgt tgctgtccac gagcggagcc aactgttgaa gcttcccggt
     2221 gaaacggaat cgaaagatct gagagcgatc agagaagagc gccaaaggac gcttagacag
     2281 ctccggacgc aacttgcata cttgcgcctt ctggttcgct gcggtagtga agacgttgga
     2341 agaagagaga ggtcatgggc taaactcata gagcaaccta tggatgctaa tcaaatgacg
     2401 cctgattgga gagaagcatt cgaagacgaa cttcagaaac tgaaatccct ttacgggata
     2461 tgcggcgatc gcgagtggac agaagcagtg tatgagtctg tgaggcgcgt gtggcggcat
     2521 atgggtaaac aggtgcgcga ttggagaaaa gacgttagga gcggggaaag acctaagata
     2581 cggggatatc agaaagacgt tgtcggggga aatagcattg aacagattga atatttggag
     2641 cgccaatata agttcctcaa atcctggtct ttcttcggca aagtgtcagg ccaggtgata
     2701 cgcgcggaaa agggatcgcg ctttgcaata actctgagag aacatattga tcatgccaaa
     2761 gaagatcggt tgaagaaact cgccgataga atcatcatgg aggcgcttgg ttatgtctac
     2821 gccttggacg atgaacgggg aaagggaaag tgggtcgcca agtatccacc ttgccaactc
     2881 attctcctcg aagaactttc cgaataccag tttaacaacg atcggccgcc atcagagaat
     2941 aatcaactga tgcagtggtc ccatcgcggt gtgtttcaag agttgctcaa tcaggcccaa
     3001 gtccatgatc tgcttgttgg cacaatgtat gcagcctttt cctcccggtt tgatgcaaga
     3061 acaggggctc ctggcatacg ctgtagacgg gtcccggcga ggtgcgcccg cgaacaaaac
     3121 cctgaaccgt tcccctggtg gttgaacaag ttcgttgcgg agcacaagct ggacgggtgt
     3181 cctctgcggg ccgacgatct tattcccacc ggggaagggg aattctttgt gagccctttc
     3241 tcggcggagg aaggggattt tcaccaaata catgcagatc ttaatgccgc acaaaatttg
     3301 cagaggagac tgtggtcaga ctttgatatt agtcagatac gcctccgctg tgactgggga
     3361 gaggtcgatg gcgagcctgt gttgatacca agaacgaccg gaaagaggac agccgattcg
     3421 tatggaaaca aggtttttta cacgaagacg ggcgttactt actacgaaag agaaagaggg
     3481 aagaagagaa ggaaagtctt tgcccaagaa gaattgagcg aggaagaagc cgagctcttg
     3541 gtcgaagcgg acgaggcacg ggaaaagtct gtcgtcctca tgagggaccc ttccggaatt
     3601 attaaccggg gagattggac gcggcagaaa gagttttggt ccatggttaa tcaacgcata
     3661 gaaggctacc ttgtcaagca aataagaagt cgcgtgagat tgcaggagag tgcatgtgag
     3721 aacactgggg acataaagcg tcctgctgcc accaaaaagg ccggacaggc taagaaaaag
     3781 aagggagacg gctctggatc ggggtcgggt tctggctcag tcgacttgct tgatccgggg
     3841 acaccaatgg acgcggacct ggtggcttca tcgaccgtgg tttgggaaca ggacgccgat
     3901 ccattcgccg ggaccgccga tgactttcct gcttttaatg aggaagagtt ggcttggctg
     3961 atggaactcc tgccgcaggg cggctcaggg ggtctccttg accccggcac ccccatggac
     4021 gctgacctcg ttgcaagttc gacggttgtt tgggagcaag atgcagatcc gtttgcgggt
     4081 acagctgatg actttccagc cttcaacgaa gaggagctgg catggcttat ggagcttctg
     4141 cctcaggcca gaggagggtc gggtgggctc ttggatcctg ggacgccgat ggatgccgac
     4201 cttgtcgcgt cctctactgt ggtttgggag caggacgccg acccgttcgc tggtactgct
     4261 gatgactttc cggcctttaa tgaggaggaa ctcgcttggc tcatggagtt gttgccacaa
     4321 gggggtagtg gcggtcttct ggacccggga acgcctatgg atgctgactt ggtggcatcg
     4381 agcaccgtcg tctgggaaca ggatgcggac ccgtttgctg gtaccgctga tgattttccg
     4441 gcatttaacg aggaagaatt ggcgtggctc atggaattgc ttccccaggc gagagggggt
     4501 tccggtggct tgctggatcc gggaacgcct atggatgccg atctcgtggc gtcgtcaacc
     4561 gtggtctggg agcaagacgc tgacccgttt gccggcacag cagatgattt tccagctttc
     4621 aatgaggaag aactggcatg gttgatggaa ttgcttccac agggagggag cggaggcctc
     4681 ctcgatcctg gaacacctat ggacgcagac ctggttgcct cttcaactgt cgtgtgggaa
     4741 caagatgcag acccgttcgc aggtacagct gatgacttcc ctgcctttaa tgaggaggaa
     4801 ctcgcgtggc tcatggaact tttgccccaa gcacgcggtg gctctggggg tggcggatcg
     4861 ggaggagatg cgctggacga tttcgacttg gacatgttgg gctcagacgc tttggacgac
     4921 tttgatctcg acatgcttgg gtccgacgca ttggatgatt tcgaccttga catgcttggt
     4981 tccgacgcac tcgatgactt cgatcttgat atgctcgccc gcggatctga cgctctcgac
     5041 gacttcgatc ttgacatgtt gggctcagat gcgctcgacg actttgatct cgatatgttg
     5101 ggtagcgacg cactcgatga ctttgacttg gatatgctgg ggagcgacgc cttggacgat
     5161 ttcgatctgg acatgctgga gggcagagga agtcttctaa catgcggtga cgtggaggag
     5221 aatcccggcc ctatggcgtc aaatttcacg cagtttgttt tggttgataa cggcgggact
     5281 ggcgacgtta cagtagctcc atcaaatttt gcgaacggag tcgctgagtg gattagctca
     5341 aattcaaggt cccaggccta caaggttacc tgttctgtta ggcagagttc tgcgcaaaaa
     5401 agaaaatata ccatcaaggt tgaagtccct aaagttgcaa cacaaacagt cggtggtgtt
     5461 gagctccctg tggcagcctg gagatcttac ttaaacatgg agctaacaat tccaatattc
     5521 gctacaaact ctgattgtga actgattgtt aaggcgatgc aaggtctctt gaaagatgga
     5581 aaccctatac cgtccgctat cgcagctaac agcggtatct atcctaagaa gaagaggaag
     5641 gttggctctg gatcggggtc gggttctggc tcattgcttg atccggggac accaatggac
     5701 gcggacctgg tggcttcatc gaccgtggtt tgggaacagg acgccgatcc attcgccggg
     5761 accgccgatg actttcctgc ttttaatgag gaagagttgg cttggctgat ggaactcctg
     5821 ccgcagggcg gctcaggggg tctccttgac cccggcaccc ccatggacgc tgacctcgtt
     5881 gcaagttcga cggttgtttg ggagcaagat gcagatccgt ttgcgggtac agctgatgac
     5941 tttccagcct tcaacgaaga ggagctggca tggcttatgg agcttctgcc tcaggccaga
     6001 ggagggtcgg gtgggctctt ggatcctggg acgccgatgg atgccgacct tgtcgcgtcc
     6061 tctactgtgg tttgggagca ggacgccgac ccgttcgctg gtactgctga tgactttccg
     6121 gcctttaatg aggaggaact cgcttggctc atggagttgt tgccacaagg gggtagtggc
     6181 ggtcttctgg acccgggaac gcctatggat gctgacttgg tggcatcgag caccgtcgtc
     6241 tgggaacagg atgcggaccc gtttgctggt accgctgatg attttccggc atttaacgag
     6301 gaagaattgg cgtggctcat ggaattgctt ccccaggcga gagggggttc cggtggcttg
     6361 ctggatccgg gaacgcctat ggatgccgat ctcgtggcgt cgtcaaccgt ggtctgggag
     6421 caagacgctg acccgtttgc cggcacagca gatgattttc cagctttcaa tgaggaagaa
     6481 ctggcatggt tgatggaatt gcttccacag ggagggagcg gaggcctcct cgatcctgga
     6541 acacctatgg acgcagacct ggttgcctct tcaactgtcg tgtgggaaca agatgcagac
     6601 ccgttcgcag gtacagctga tgacttccct gcctttaatg aggaggaact cgcgtggctc
     6661 atggaacttt tgccccaagc acgcggtggc tctgggggtg gcggatcggg aggagatgcg
     6721 ctggacgatt tcgacttgga catgttgggc tcagacgctt tggacgactt tgatctcgac
     6781 atgcttgggt ccgacgcatt ggatgatttc gaccttgaca tgcttggttc cgacgcactc
     6841 gatgacttcg atcttgatat gctcgcccgc ggatctgacg ctctcgacga cttcgatctt
     6901 gacatgttgg gctcagatgc gctcgacgac tttgatctcg atatgttggg tagcgacgca
     6961 ctcgatgact ttgacttgga tatgctgggg agcgacgcct tggacgattt cgatctggac
     7021 atgctgtgat gataggacgt ccgatcgttc aaacatttgg caataaagtt tcttaagatt
     7081 gaatcctgtt gccggtcttg cgatgattat catataattt ctgttgaatt acgttaagca
     7141 tgtaataatt aacatgtaat gcatgacgtt atttatgaga tgggttttta tgattagagt
     7201 cccgcaatta tacatttaat acgcgataga aaacaaaata tagcgcgcaa actaggataa
     7261 attatcgcgc gcggtgtcat ctatgttact agatcgggaa ttgatccccc ctcgacagct
     7321 tccggaaagg gcgaattcgc aactttgtat acaaaagttg aacgagaaac gtaaaatgat
     7381 ataaatatca atatattaaa ttagattttg cataaaaaac agactacata atactgtaaa
     7441 acacaacata tccagtcact atgccatcca gctgatatcc cctatagtga gtcgtattac
     7501 atggtcatag ctgtttcctg gcagctctgg cccgtgtctc aaaatctctg atgttacatt
     7561 gcacaagata aaaatatatc atcatgcctc ctctggacca gccaggacag aaatgcctcg
     7621 acttcgctgc tacccaaggt tgccgggtga cgcacaccgt ggaaacggat gaaggcacga
     7681 acccagtgga cataagcctg ttcggttcgt aagctgtaat gcaagtagcg tatgcgctca
     7741 cgcaactggt ccagaacctt gaccgaacgc agcggtggta acggcgcagt ggcggttttc
     7801 atggcttgtt atgactgttt ttttggggta cagtctatgc ctcgggcatc caagcagcaa
     7861 gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca gcaacgatgt tacgcagcag
     7921 ggcagtcgcc ctaaaacaaa gttaaacatt atgagggaag cggtgatcgc cgaagtatcg
     7981 actcaactat cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc
     8041 gtacatttgt acggctccgc agtggatggc ggcctgaagc cacacagtga tattgatttg
     8101 ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc gagctttgat caacgacctt
     8161 ttggaaactt cggcttcccc tggagagagc gagattctcc gcgctgtaga agtcaccatt
     8221 gttgtgcacg acgacatcat tccgtggcgt tatccagcta agcgcgaact gcaatttgga
     8281 gaatggcagc gcaatgacat tcttgcaggt atcttcgagc cagccacgat cgacattgat
     8341 ctggctatct tgctgacaaa agcaagagaa catagcgttg ccttggtagg tccagcggcg
     8401 gaggaactct ttgatccggt tcctgaacag gatctatttg aggcgctaaa tgaaacctta
     8461 acgctatgga actcgccgcc cgactgggct ggcgatgagc gaaatgtagt gcttacgttg
     8521 tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac
     8581 tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca tacttgaagc tagacaggct
     8641 tatcttggac aagaagaaga tcgcttggcc tcgcgcgcag atcagttgga agaatttgtc
     8701 cactacgtga aaggcgagat caccaaggta gtcggcaaat aaccctcgag ccacccatga
     8761 ccaaaatccc ttaacgtgag ttacgcgtcg ttccactgag cgtcagaccc cgtagaaaag
     8821 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     8881 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     8941 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     9001 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     9061 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     9121 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     9181 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     9241 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     9301 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     9361 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     9421 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     9481 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     9541 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     9601 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
     9661 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatacgcgt
     9721 accgcgagcc aggaagagtt tgtagaaacg caaaaaggcc atccgtcagg atggccttct
     9781 gcttagtttg atgcctggca gtttatggcg ggcgtcctgc ccgccaccct ccgggccgtt
     9841 gcttcacaac gttcaaatcc gctcccggcg g
//
