LOCUS       sequence-271514-        9748 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CRISPRa Gateway entry clone for catalytically dead AaCas12b (D570A) 
            fused with TV activation system, followed by T2A and MCP-VPR fusion 
            protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9748)
  AUTHORS   Ming M, Ren Q, Pan C, He Y, Zhang Y, Liu S, Zhong Z, Wang J, Malzahn
            AA, Wu J, Zheng X, Zhang Y, Qi Y
  TITLE     CRISPR-Cas12b enables efficient plant genome engineering.
  JOURNAL   Nat Plants. 2020 Mar;6(3):202-208. doi: 10.1038/s41477-020-0614-6. 
            Epub 2020 Mar 9.
  PUBMED    32170285
REFERENCE   2  (bases 1 to 9748)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41477-020-0614-6"; journalName: "Nat Plants"; date: 
            "2020-03"; volume: "6"; issue: "3"; pages: "202-208"
COMMENT     Sequence Label: pYPQ292 (AaCas12b)-D570A-TV-MS2-VPR
FEATURES             Location/Qualifiers
     source          1..9748
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             125..145
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3554..3601
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             4685..4834
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             4847..4996
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             4997..5050
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5054..5440
                     /codon_start=1
                     /product="bacteriophage MS2 coat protein"
                     /label=MS2-N55K
                     /note="binds with high affinity to a specific stem-loop 
                     structure in the viral RNA (Lim et al., 1994)"
                     /translation="ASNFTQFVLVDNGGTGDVTVAPSNFANGVAEWISSNSRSQAYKVT
                     CSVRQSSAQKRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATNSDCELI
                     VKAMQGLLKDGNPIPSAIAANSGIY"
     CDS             5441..5461
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5504..5524
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5528..5548
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5552..5572
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5573..5722
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             5723..5743
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5747..5767
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5768..6124
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALL"
     CDS             6149..6718
                     /codon_start=1
                     /product="transcriptional activation domain from the human 
                     herpesvirus 4 (Epstein-Barr virus) replication and 
                     transcription activator Rta/BRLF1 (Hardwick et al., 1992; 
                     Chavez et al., 2015)"
                     /label=Rta AD
                     /translation="RDSREGMFLPKPEAGSAISDVFEGREVCQPKRIRPFHPPGSPWAN
                     RPLPASLAPTPTGPVHEPVGSLTPAPVPQPLDPAPAVTPEASHLLEDPDEETSQAVKAL
                     REMADTVIPQKEEAAICGQMDLSHPPPRGHLDELTTTLESMTEDLNLDSPLTPELNEIL
                     DTFLNDECLLHAMHISTGLSIFDTSLF"
     terminator      6738..6990
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    7035..7158
                     /gene="mutant version of attR"
                     /label=attR5
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(7175..7194)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7176..7194)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7199..7215)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7199..7215)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             7646..8437
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      8530..9118
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9019..9038
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9272..9289
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(9448..9475)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(9567..9653)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     9702..9724
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9716..9733
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9717..9733
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgaattcgc ccttcaccat
      121 ggctcctaag aagaagcgga aggttggtat tcacggggtg cctgcggctg ccgtcaagtc
      181 catgaaggtc aagttgcgcc tggataacat gccagagatc agagccggac tttggaaact
      241 tcacaccgag gttaatgcgg gtgtgcggta ctatacggaa tggcttagcc ttttgaggca
      301 agaaaatctt tatcggagga gtcccaatgg cgatggagaa caagaatgct ataaaactgc
      361 tgaggaatgc aaggctgaac tccttgagag actcagagcc cgccaagttg agaatgggca
      421 ctgcggccct gctgggagtg atgacgaact gctgcaattg gcacggcaac tttatgaact
      481 tctggtccca caagcaatcg gggctaaagg tgatgcgcag caaatcgcaa ggaagtttct
      541 tagtcccctt gccgacaagg atgccgtggg tggtttggga atagcaaaag caggaaataa
      601 gcctaggtgg gttcggatga gggaggctgg agagccaggt tgggaagagg aaaaggctaa
      661 agccgaggcg agaaagagta cggatagaac cgccgatgtt cttcgcgctc ttgcagactt
      721 cggtcttaaa cctcttatga gagtctacac agactcagac atgtccagcg tgcagtggaa
      781 accacttcgc aaaggacaag cggtcagaac ctgggataga gacatgttcc aacaagcgat
      841 cgaaagaatg atgagttggg aatcgtggaa tcagcgcgtt ggagaagcgt acgcaaagct
      901 cgtggaacaa aagtcgaggt ttgaacagaa aaattttgtg ggacaagaac atcttgtcca
      961 acttgtcaat caacttcaac aagacatgaa ggaagcatca cacggcctgg agtcgaaaga
     1021 acaaactgcg cattacttga ctgggagagc gctgagaggg agcgacaaag tttttgagaa
     1081 gtgggaaaaa ctcgatcctg atgccccatt tgacctctat gataccgaaa tcaagaatgt
     1141 tcaacggagg aatactcgca ggttcggatc tcatgatctg tttgcgaagc tcgcggaacc
     1201 taaatatcag gcgctctgga gagaggacgc ttctttcctc acgaggtatg cggtttacaa
     1261 tagcattgtc agaaaactga atcacgctaa aatgtttgcg acttttactc ttccggatgc
     1321 taccgcccac ccgatctgga cgcggtttga caaactcggc ggcaacctgc accagtacac
     1381 tttcttgttt aacgaatttg gcgagggcag gcacgccatt cggtttcaga agctgttgac
     1441 ggttgaggat ggcgttgcta aagaggtcga cgacgtcacg gttccgattt ctatgtccgc
     1501 gcagctggat gacctcttgc ctcgggaccc acacgagctc gttgcactct acttccagga
     1561 ctacggtgca gaacaacatc tggctggaga gtttggcggc gcgaaaattc aataccgccg
     1621 cgatcaattg aaccacctgc acgccagaag aggcgccaga gatgtctacc ttaatctgag
     1681 cgtccgcgtt cagtcacaat ccgaagccag gggagaaagg cgccctccgt atgcagcggt
     1741 cttcaggctt gttggcgata accaccgcgc gtttgttcac tttgataaat tgtcagatta
     1801 cctcgcagaa cacccagacg atggtaagct ggggtcggaa ggtttgctct ctgggctcag
     1861 agtcatgtca gttgccttgg gtcttaggac ttccgcgagc atatctgtct tccgcgtcgc
     1921 aagaaaggac gaattgaagc cgaacagtga aggccgggtc cctttttgct tcccgatcga
     1981 agggaacgaa aacctcgttg ctgtccacga gcggagccaa ctgttgaagc ttcccggtga
     2041 aacggaatcg aaagatctga gagcgatcag agaagagcgc caaaggacgc ttagacagct
     2101 ccggacgcaa cttgcatact tgcgccttct ggttcgctgc ggtagtgaag acgttggaag
     2161 aagagagagg tcatgggcta aactcataga gcaacctatg gatgctaatc aaatgacgcc
     2221 tgattggaga gaagcattcg aagacgaact tcagaaactg aaatcccttt acgggatatg
     2281 cggcgatcgc gagtggacag aagcagtgta tgagtctgtg aggcgcgtgt ggcggcatat
     2341 gggtaaacag gtgcgcgatt ggagaaaaga cgttaggagc ggggaaagac ctaagatacg
     2401 gggatatcag aaagacgttg tcgggggaaa tagcattgaa cagattgaat atttggagcg
     2461 ccaatataag ttcctcaaat cctggtcttt cttcggcaaa gtgtcaggcc aggtgatacg
     2521 cgcggaaaag ggatcgcgct ttgcaataac tctgagagaa catattgatc atgccaaaga
     2581 agatcggttg aagaaactcg ccgatagaat catcatggag gcgcttggtt atgtctacgc
     2641 cttggacgat gaacggggaa agggaaagtg ggtcgccaag tatccacctt gccaactcat
     2701 tctcctcgaa gaactttccg aataccagtt taacaacgat cggccgccat cagagaataa
     2761 tcaactgatg cagtggtccc atcgcggtgt gtttcaagag ttgctcaatc aggcccaagt
     2821 ccatgatctg cttgttggca caatgtatgc agccttttcc tcccggtttg atgcaagaac
     2881 aggggctcct ggcatacgct gtagacgggt cccggcgagg tgcgcccgcg aacaaaaccc
     2941 tgaaccgttc ccctggtggt tgaacaagtt cgttgcggag cacaagctgg acgggtgtcc
     3001 tctgcgggcc gacgatctta ttcccaccgg ggaaggggaa ttctttgtga gccctttctc
     3061 ggcggaggaa ggggattttc accaaataca tgcagatctt aatgccgcac aaaatttgca
     3121 gaggagactg tggtcagact ttgatattag tcagatacgc ctccgctgtg actggggaga
     3181 ggtcgatggc gagcctgtgt tgataccaag aacgaccgga aagaggacag ccgattcgta
     3241 tggaaacaag gttttttaca cgaagacggg cgttacttac tacgaaagag aaagagggaa
     3301 gaagagaagg aaagtctttg cccaagaaga attgagcgag gaagaagccg agctcttggt
     3361 cgaagcggac gaggcacggg aaaagtctgt cgtcctcatg agggaccctt ccggaattat
     3421 taaccgggga gattggacgc ggcagaaaga gttttggtcc atggttaatc aacgcataga
     3481 aggctacctt gtcaagcaaa taagaagtcg cgtgagattg caggagagtg catgtgagaa
     3541 cactggggac ataaagcgtc ctgctgccac caaaaaggcc ggacaggcta agaaaaagaa
     3601 gggagacggc tctggatcgg ggtcgggttc tggctcagtc gacttgcttg atccggggac
     3661 accaatggac gcggacctgg tggcttcatc gaccgtggtt tgggaacagg acgccgatcc
     3721 attcgccggg accgccgatg actttcctgc ttttaatgag gaagagttgg cttggctgat
     3781 ggaactcctg ccgcagggcg gctcaggggg tctccttgac cccggcaccc ccatggacgc
     3841 tgacctcgtt gcaagttcga cggttgtttg ggagcaagat gcagatccgt ttgcgggtac
     3901 agctgatgac tttccagcct tcaacgaaga ggagctggca tggcttatgg agcttctgcc
     3961 tcaggccaga ggagggtcgg gtgggctctt ggatcctggg acgccgatgg atgccgacct
     4021 tgtcgcgtcc tctactgtgg tttgggagca ggacgccgac ccgttcgctg gtactgctga
     4081 tgactttccg gcctttaatg aggaggaact cgcttggctc atggagttgt tgccacaagg
     4141 gggtagtggc ggtcttctgg acccgggaac gcctatggat gctgacttgg tggcatcgag
     4201 caccgtcgtc tgggaacagg atgcggaccc gtttgctggt accgctgatg attttccggc
     4261 atttaacgag gaagaattgg cgtggctcat ggaattgctt ccccaggcga gagggggttc
     4321 cggtggcttg ctggatccgg gaacgcctat ggatgccgat ctcgtggcgt cgtcaaccgt
     4381 ggtctgggag caagacgctg acccgtttgc cggcacagca gatgattttc cagctttcaa
     4441 tgaggaagaa ctggcatggt tgatggaatt gcttccacag ggagggagcg gaggcctcct
     4501 cgatcctgga acacctatgg acgcagacct ggttgcctct tcaactgtcg tgtgggaaca
     4561 agatgcagac ccgttcgcag gtacagctga tgacttccct gcctttaatg aggaggaact
     4621 cgcgtggctc atggaacttt tgccccaagc acgcggtggc tctgggggtg gcggatcggg
     4681 aggagatgcg ctggacgatt tcgacttgga catgttgggc tcagacgctt tggacgactt
     4741 tgatctcgac atgcttgggt ccgacgcatt ggatgatttc gaccttgaca tgcttggttc
     4801 cgacgcactc gatgacttcg atcttgatat gctcgcccgc ggatctgacg ctctcgacga
     4861 cttcgatctt gacatgttgg gctcagatgc gctcgacgac tttgatctcg atatgttggg
     4921 tagcgacgca ctcgatgact ttgacttgga tatgctgggg agcgacgcct tggacgattt
     4981 cgatctggac atgctggagg gcagaggaag tcttctaaca tgcggtgacg tggaggagaa
     5041 tcccggccct atggcgtcaa atttcacgca gtttgttttg gttgataacg gcgggactgg
     5101 cgacgttaca gtagctccat caaattttgc gaacggagtc gctgagtgga ttagctcaaa
     5161 ttcaaggtcc caggcctaca aggttacctg ttctgttagg cagagttctg cgcaaaaaag
     5221 aaaatatacc atcaaggttg aagtccctaa agttgcaaca caaacagtcg gtggtgttga
     5281 gctccctgtg gcagcctgga gatcttactt aaacatggag ctaacaattc caatattcgc
     5341 tacaaactct gattgtgaac tgattgttaa ggcgatgcaa ggtctcttga aagatggaaa
     5401 ccctataccg tccgctatcg cagctaacag cggtatctat cctaagaaga agaggaaggt
     5461 tggctctgga tcggggtcgg gttctggctc aggatccggt accccaaaaa agaagagaaa
     5521 ggtcgacccg aagaagaaaa ggaaagtcga ccctaaaaaa aaacgcaaag ttgatgcgct
     5581 tgacgacttt gacttggaca tgttgggatc agatgccctc gacgactttg atctcgatat
     5641 gcttggttcg gacgcgcttg acgatttcga tcttgacatg ctcggttctg atgcacttga
     5701 tgacttcgat ttggacatgc tgcccaaaaa gaaaagaaag gtggatccaa agaagaaacg
     5761 caaagtccct actcaggctg gggaagggac tctgagtgaa gctctcctgc aacttcagtt
     5821 cgacgacgag gacttgggtg cccttctcgg gaacagcacc gacccggctg ttttcactga
     5881 cctcgcgtct gttgataatt ccgaattcca acagcttttg aatcaaggaa ttcctgttgc
     5941 cccacatact actgaaccca tgctgatgga atatcccgaa gcaataacta gactggtgac
     6001 cggtgcgcag cggccgccag acccagctcc agccccgctg ggtgcaccag gactgccgaa
     6061 tggcttgctg tcaggcgatg aagactttag ttcgatcgct gatatggact tctctgcact
     6121 gcttattaat tccaggtctt ctggtagtcg ggactctcgc gagggtatgt tcttgccaaa
     6181 accagaggct ggatcggcaa tatctgatgt ttttgaggga cgggaggtct gccagccgaa
     6241 acgcatccgc ccgtttcacc cgccaggtag cccctgggca aaccgcccat tgcccgcctc
     6301 gcttgcccca acccccaccg gtcctgttca cgaacccgtc ggatctctga ccccagcccc
     6361 tgttccacag ccgttggacc cagccccagc agtcaccccg gaggccagtc atctcctgga
     6421 ggacccagat gaagagacca gtcaggcagt taaggctttg cgggaaatgg ctgacaccgt
     6481 catacctcag aaagaagaag cagccatttg cggacagatg gatctgtctc atccccctcc
     6541 ccggggtcac cttgatgagc tgacaacaac gctggagagt atgacggaag atcttaactt
     6601 ggactcacct ctgaccccgg aactcaacga aatattggat acatttttga acgatgagtg
     6661 tctgcttcat gcaatgcaca tcagtaccgg actgagtata tttgatacta gcttgtttaa
     6721 gctttgatag gacgtccgat cgttcaaaca tttggcaata aagtttctta agattgaatc
     6781 ctgttgccgg tcttgcgatg attatcatat aatttctgtt gaattacgtt aagcatgtaa
     6841 taattaacat gtaatgcatg acgttattta tgagatgggt ttttatgatt agagtcccgc
     6901 aattatacat ttaatacgcg atagaaaaca aaatatagcg cgcaaactag gataaattat
     6961 cgcgcgcggt gtcatctatg ttactagatc gggaattgat cccccctcga cagcttccgg
     7021 aaagggcgaa ttcgcaactt tgtatacaaa agttgaacga gaaacgtaaa atgatataaa
     7081 tatcaatata ttaaattaga ttttgcataa aaaacagact acataatact gtaaaacaca
     7141 acatatccag tcactatgcc atccagctga tatcccctat agtgagtcgt attacatggt
     7201 catagctgtt tcctggcagc tctggcccgt gtctcaaaat ctctgatgtt acattgcaca
     7261 agataaaaat atatcatcat gcctcctctg gaccagccag gacagaaatg cctcgacttc
     7321 gctgctaccc aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca
     7381 gtggacataa gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa
     7441 ctggtccaga accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc
     7501 ttgttatgac tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg
     7561 ttacgccgtg ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag
     7621 tcgccctaaa acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca
     7681 actatcagag gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca
     7741 tttgtacggc tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt
     7801 tacggtgacc gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga
     7861 aacttcggct tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt
     7921 gcacgacgac atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg
     7981 gcagcgcaat gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc
     8041 tatcttgctg acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga
     8101 actctttgat ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct
     8161 atggaactcg ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg
     8221 catttggtac agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc
     8281 aatggagcgc ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct
     8341 tggacaagaa gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta
     8401 cgtgaaaggc gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa
     8461 atcccttaac gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     8521 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     8581 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     8641 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     8701 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     8761 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     8821 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     8881 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     8941 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     9001 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     9061 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     9121 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     9181 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     9241 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     9301 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
     9361 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
     9421 gagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
     9481 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
     9541 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
     9601 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
     9661 gcagttccct actctcgcgt taacgcttgc atggatgttt tcccagtcac gacgttgtaa
     9721 aacgacggcc agtcttaagc tcgggccc
//
