LOCUS       sequence-271520-       10367 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level1 ZmUBIp::LbCas12a (human codon optimized):NOSt.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10367)
  AUTHORS   Hahn F, Korolev A, Sanjurjo Loures L, Nekrasov V
  TITLE     A modular cloning toolkit for genome editing in plants.
  JOURNAL   BMC Plant Biol. 2020 Apr 23;20(1):179. doi: 
            10.1186/s12870-020-02388-2.
  PUBMED    32326901
REFERENCE   2  (bases 1 to 10367)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Plant 
            Biol."; date: "2020-04-23"; volume: "20(1):179. doi"; pages: " 
            10.1186/s12870-020-02388-2"
COMMENT     Sequence Label: pFH133
FEATURES             Location/Qualifiers
     source          1..10367
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2023..5706
                     /codon_start=1
                     /product="CRISPR-associated protein from Lachnospiraceae 
                     bacterium ND2006 (Zetsche et al., 2015)"
                     /label=LbCpf1
                     /translation="MSKLEKFTNCYSLSKTLRFKAIPVGKTQENIDNKRLLVEDEKRAE
                     DYKGVKKLLDRYYLSFINDVLHSIKLKNLNNYISLFRKKTRTEKENKELENLEINLRKE
                     IAKAFKGNEGYKSLFKKDIIETILPEFLDDKDEIALVNSFNGFTTAFTGFFDNRENMFS
                     EEAKSTSIAFRCINENLTRYISNMDIFEKVDAIFDKHEVQEIKEKILNSDYDVEDFFEG
                     EFFNFVLTQEGIDVYNAIIGGFVTESGEKIKGLNEYINLYNQKTKQKLPKFKPLYKQVL
                     SDRESLSFYGEGYTSDEEVLEVFRNTLNKNSEIFSSIKKLEKLFKNFDEYSSAGIFVKN
                     GPAISTISKDIFGEWNVIRDKWNAEYDDIHLKKKAVVTEKYEDDRRKSFKKIGSFSLEQ
                     LQEYADADLSVVEKLKEIIIQKVDEIYKVYGSSEKLFDADFVLEKSLKKNDAVVAIMKD
                     LLDSVKSFENYIKAFFGEGKETNRDESFYGDFVLAYDILLKVDHIYDAIRNYVTQKPYS
                     KDKFKLYFQNPQFMGGWDKDKETDYRATILRYGSKYYLAIMDKKYAKCLQKIDKDDVNG
                     NYEKINYKLLPGPNKMLPKVFFSKKWMAYYNPSEDIQKIYKNGTFKKGDMFNLNDCHKL
                     IDFFKDSISRYPKWSNAYDFNFSETEKYKDIAGFYREVEEQGYKVSFESASKKEVDKLV
                     EEGKLYMFQIYNKDFSDKSHGTPNLHTMYFKLLFDENNHGQIRLSGGAELFMRRASLKK
                     EELVVHPANSPIANKNPDNPKKTTTLSYDVYKDKRFSEDQYELHIPIAINKCPKNIFKI
                     NTEVRVLLKHDDNPYVIGIDRGERNLLYIVVVDGKGNIVEQYSLNEIINNFNGIRIKTD
                     YHSLLDKKEKERFEARQNWTSIENIKELKAGYISQVVHKICELVEKYDAVIALEDLNSG
                     FKNSRVKVEKQVYQKFEKMLIDKLNYMVDKKSNPCATGGALKGYQITNKFESFKSMSTQ
                     NGFIFYIPAWLTSKIDPSTGFVNLLKTKYTSIADSKKFISSFDRIMYVPEEDLFEFALD
                     YKNFSRTDADYIKKWKLYSYGNRIRIFRNPKKNNVFDWEEVCLTSAYKELFNKYGINYQ
                     QGDIRALLCEQSDKAFYSSFMALMSLMLQMRNSITGRTDVDFLISPVKNSDGIFYDSRN
                     YEAQENAILPKNADANGAYNIARKVLWAIGQFKKAEDEKLDKVKIAISNKEWLEYAQTS
                     VKH"
     CDS             5707..5754
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     terminator      5770..6022
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    6070..6094
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             complement(6250..7398)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     rep_origin      complement(7734..8322)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7814..7833)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8493..9284,9285..9353))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9116..9135
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9354..9458)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      9545..10176
                     /label=oriV
                     /note="incP origin of replication"
     misc_feature    10268..10292
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 tctgtgaaga caagcaagaa ttcaagcttg gaggtgcagc gtgacccggt cgtgcccctc
       61 tctagagata atgagcattg catgtctaag ttataaaaaa ttaccacata ttttttttgt
      121 cacacttgtt tgaagtgcag tttatctatc tttatacata tatttaaact ttactctacg
      181 aataatataa tctatagtac tacaataata tcagtgtttt agagaatcat ataaatgaac
      241 agttagacat ggtctaaagg acaattgagt attttgacaa caggactcta cagttttatc
      301 tttttagtgt gcatgtgttc tccttttttt ttgcaaatag cttcacctat ataatacttc
      361 atccatttta ttagtacatc catttagggt ttagggttaa tggtttttat agactaattt
      421 ttttagtaca tctattttat tctattttag cctctaaatt aagaaaacta aaactctatt
      481 ttagtttttt tatttaataa tttagatata aaatagaata aaataaagtg actaaaaatt
      541 aaacaaatac cctttaagaa attaaaaaaa ctaaggaaac atttttcttg tttcgagtag
      601 ataatgccag cctgttaaac gccgtcgacg agtctaacgg acaccaacca gcgaaccagc
      661 agcgtcgcgt cgggccaagc gaagcagacg gcacggcatc tctgtcgctg cctctggacc
      721 cctctcgaga gttccgctcc accgttggac ttgctccgct gtcggcatcc agaaattgcg
      781 tggcggagcg gcagacgtga gccggcacgg caggcggcct cctcctcctc tcacggcacg
      841 gcagctacgg gggattcctt tcccaccgct ccttcgcttt cccttcctcg cccgccgtaa
      901 taaatagaca ccccctccac accctctttc cccaacctcg tgttgttcgg agcgcacaca
      961 cacacaacca gatctccccc aaatccaccc gtcggcacct ccgcttcaag gtacgccgct
     1021 cgtcctcccc ccccccccct ctctaccttc tctagatcgg cgttccggtc catggttagg
     1081 gcccggtagt tctacttctg ttcatgtttg tgttagatcc gtgtttgtgt tagatccgtg
     1141 ctgctagcgt tcgtacacgg atgcgacctg tacgtcagac acgttctgat tgctaacttg
     1201 ccagtgtttc tctttgggga atcctgggat ggctctagcc gttccgcaga cgggatcgat
     1261 ttcatgattt tttttgtttc gttgcatagg gtttggtttg cccttttcct ttatttcaat
     1321 atatgccgtg cacttgtttg tcgggtcatc ttttcatgct tttttttgtc ttggttgtga
     1381 tgatgtggtc tggttgggcg gtcgttctag atcggagtag aattctgttt caaactacct
     1441 ggtggattta ttaattttgg atctgtatgt gtgtgccata catattcata gttacgaatt
     1501 gaagatgatg gatggaaata tcgatctagg ataggtatac atgttgatgc gggttttact
     1561 gatgcatata cagagatgct ttttgttcgc ttggttgtga tgatgtggtg tggttgggcg
     1621 gtcgttcatt cgttctagat cggagtagaa tactgtttca aactacctgg tgtatttatt
     1681 aattttggaa ctgtatgtgt gtgtcataca tcttcatagt tacgagttta agatggatgg
     1741 aaatatcgat ctaggatagg tatacatgtt gatgtgggtt ttactgatgc atatacatga
     1801 tggcatatgc agcatctatt catatgctct aaccttgagt acctatctat tataataaac
     1861 aagtatgttt tataattatt ttgatcttga tatacttgga tgatggcata tgcagcagct
     1921 atatgtggat ttttttagcc ctgccttcat acgctattta tttgcttggt actgtttctt
     1981 ttgtcgatgc tcaccctgtt gtttggtgtt acttctgcag gaatgagcaa gctggagaag
     2041 tttacaaact gctactccct gtctaagacc ctgaggttca aggccatccc tgtgggcaag
     2101 acccaggaga acatcgacaa taagcggctg ctggtggagg acgagaagag agccgaggat
     2161 tataagggcg tgaagaagct gctggatcgc tactatctgt cttttatcaa cgacgtgctg
     2221 cacagcatca agctgaagaa tctgaacaat tacatcagcc tgttccggaa gaaaaccaga
     2281 accgagaagg agaataagga gctggagaac ctggagatca atctgcggaa ggagatcgcc
     2341 aaggccttca agggcaacga gggctacaag tccctgttta agaaggatat catcgagaca
     2401 atcctgccag agttcctgga cgataaggac gagatcgccc tggtgaacag cttcaatggc
     2461 tttaccacag ccttcaccgg cttctttgat aacagagaga atatgttttc cgaggaggcc
     2521 aagagcacat ccatcgcctt caggtgtatc aacgagaatc tgacccgcta catctctaat
     2581 atggacatct tcgagaaggt ggacgccatc tttgataagc acgaggtgca ggagatcaag
     2641 gagaagatcc tgaacagcga ctatgatgtg gaggatttct ttgagggcga gttctttaac
     2701 tttgtgctga cacaggaggg catcgacgtg tataacgcca tcatcggcgg cttcgtgacc
     2761 gagagcggcg agaagatcaa gggcctgaac gagtacatca acctgtataa tcagaaaacc
     2821 aagcagaagc tgcctaagtt taagccactg tataagcagg tgctgagcga tcgggagtct
     2881 ctgagcttct acggcgaggg ctatacatcc gatgaggagg tgctggaggt gtttagaaac
     2941 accctgaaca agaacagcga gatcttcagc tccatcaaga agctggagaa gctgttcaag
     3001 aattttgacg agtactctag cgccggcatc tttgtgaaga acggccccgc catcagcaca
     3061 atctccaagg atatcttcgg cgagtggaac gtgatccggg acaagtggaa tgccgagtat
     3121 gacgatatcc acctgaagaa gaaggccgtg gtgaccgaga agtacgagga cgatcggaga
     3181 aagtccttca agaagatcgg ctccttttct ctggagcagc tgcaggagta cgccgacgcc
     3241 gatctgtctg tggtggagaa gctgaaggag atcatcatcc agaaggtgga tgagatctac
     3301 aaggtgtatg gctcctctga gaagctgttc gacgccgatt ttgtgctgga gaagagcctg
     3361 aagaagaacg acgccgtggt ggccatcatg aaggacctgc tggattctgt gaagagcttc
     3421 gagaattaca tcaaggcctt ctttggcgag ggcaaggaga caaacaggga cgagtccttc
     3481 tatggcgatt ttgtgctggc ctacgacatc ctgctgaagg tggaccacat ctacgatgcc
     3541 atccgcaatt atgtgaccca gaagccctac tctaaggata agttcaagct gtattttcag
     3601 aaccctcagt tcatgggcgg ctgggacaag gataaggaga cagactatcg ggccaccatc
     3661 ctgagatacg gctccaagta ctatctggcc atcatggata agaagtacgc caagtgcctg
     3721 cagaagatcg acaaggacga tgtgaacggc aattacgaga agatcaacta taagctgctg
     3781 cccggcccta ataagatgct gccaaaggtg ttcttttcta agaagtggat ggcctactat
     3841 aaccccagcg aggacatcca gaagatctac aagaatggca cattcaagaa gggcgatatg
     3901 tttaacctga atgactgtca caagctgatc gacttcttta aggatagcat ctcccggtat
     3961 ccaaagtggt ccaatgccta cgatttcaac ttttctgaga cagagaagta taaggacatc
     4021 gccggctttt acagagaggt ggaggagcag ggctataagg tgagcttcga gtctgccagc
     4081 aagaaggagg tggataagct ggtggaggag ggcaagctgt atatgttcca gatctataac
     4141 aaggactttt ccgataagtc tcacggcaca cccaatctgc acaccatgta cttcaagctg
     4201 ctgtttgacg agaacaatca cggacagatc aggctgagcg gaggagcaga gctgttcatg
     4261 aggcgcgcct ccctgaagaa ggaggagctg gtggtgcacc cagccaactc ccctatcgcc
     4321 aacaagaatc cagataatcc caagaaaacc acaaccctgt cctacgacgt gtataaggat
     4381 aagaggtttt ctgaggacca gtacgagctg cacatcccaa tcgccatcaa taagtgcccc
     4441 aagaacatct tcaagatcaa tacagaggtg cgcgtgctgc tgaagcacga cgataacccc
     4501 tatgtgatcg gcatcgatag gggcgagcgc aatctgctgt atatcgtggt ggtggacggc
     4561 aagggcaaca tcgtggagca gtattccctg aacgagatca tcaacaactt caacggcatc
     4621 aggatcaaga cagattacca ctctctgctg gacaagaagg agaaggagag gttcgaggcc
     4681 cgccagaact ggacctccat cgagaatatc aaggagctga aggccggcta tatctctcag
     4741 gtggtgcaca agatctgcga gctggtggag aagtacgatg ccgtgatcgc cctggaggac
     4801 ctgaactctg gctttaagaa tagccgcgtg aaggtggaga agcaggtgta tcagaagttc
     4861 gagaagatgc tgatcgataa gctgaactac atggtggaca agaagtctaa tccttgtgca
     4921 acaggcggcg ccctgaaggg ctatcagatc accaataagt tcgagagctt taagtccatg
     4981 tctacccaga acggcttcat cttttacatc cctgcctggc tgacatccaa gatcgatcca
     5041 tctaccggct ttgtgaacct gctgaaaacc aagtatacca gcatcgccga ttccaagaag
     5101 ttcatcagct cctttgacag gatcatgtac gtgcccgagg aggatctgtt cgagtttgcc
     5161 ctggactata agaacttctc tcgcacagac gccgattaca tcaagaagtg gaagctgtac
     5221 tcctacggca accggatcag aatcttccgg aatcctaaga agaacaacgt gttcgactgg
     5281 gaggaggtgt gcctgaccag cgcctataag gagctgttca acaagtacgg catcaattat
     5341 cagcagggcg atatcagagc cctgctgtgc gagcagtccg acaaggcctt ctactctagc
     5401 tttatggccc tgatgagcct gatgctgcag atgcggaaca gcatcacagg ccgcaccgac
     5461 gtggattttc tgatcagccc tgtgaagaac tccgacggca tcttctacga tagccggaac
     5521 tatgaggccc aggagaatgc catcctgcca aagaacgccg acgccaatgg cgcctataac
     5581 atcgccagaa aggtgctgtg ggccatcggc cagttcaaga aggccgagga cgagaagctg
     5641 gataaggtga agatcgccat ctctaacaag gagtggctgg agtacgccca gaccagcgtg
     5701 aagcacaaaa ggccggcggc cacgaaaaag gccggccagg caaaaaagaa aaagtgagct
     5761 tgtcaagcag atcgttcaaa catttggcaa taaagtttct taagattgaa tcctgttgcc
     5821 ggtcttgcga tgattatcat ataatttctg ttgaattacg ttaagcatgt aataattaac
     5881 atgtaatgca tgacgttatt tatgagatgg gtttttatga ttagagtccc gcaattatac
     5941 atttaatacg cgatagaaaa caaaatatag cgcgcaaact aggataaatt atcgcgcgcg
     6001 gtgtcatcta tgttactaga tcgacgctac tattgtcttc tgcacgaagt ggtttaaact
     6061 atcagtgttt gacaggatat attggcgggt aaacctaaga gaaaagagcg tttattagaa
     6121 taatcggata tttaaaaggg cgtgaaaagg tttatccgtt cgtccatttg tatgtgcatg
     6181 ccaaccacag ggttccccag atcaggcgct ggctgctgaa cccccagccg gaactgaccc
     6241 cacaaggccc tagcgtttgc aatgcaccag gtcatcattg acccaggcgt gttccaccag
     6301 gccgctgcct cgcaactctt cgcaggcttc gccgacctgc tcgcgccact tcttcacgcg
     6361 ggtggaatcc gatccgcaca tgaggcggaa ggtttccagc ttgagcgggt acggctcccg
     6421 gtgcgagctg aaatagtcga acatccgtcg ggccgtcggc gacagcttgc ggtacttctc
     6481 ccatatgaat ttcgtgtagt ggtcgccagc aaacagcacg acgatttcct cgtcgatcag
     6541 gacctggcaa cgggacgttt tcttgccacg gtccaggacg cggaagcggt gcagcagcga
     6601 caccgattcc aggtgcccaa cgcggtcgga cgtgaagccc atcgccgtcg cctgtaggcg
     6661 cgacaggcat tcctcggcct tcgtgtaata ccggccattg atcgaccagc ccaggtcctg
     6721 gcaaagctcg tagaacgtga aggtgatcgg ctcgccgata ggggtgcgct tcgcgtactc
     6781 caacacctgc tgccacacca gttcgtcatc gtcggcccgc agctcgacgc cggtgtaggt
     6841 gatcttcacg tccttgttga cgtggaaaat gaccttgttt tgcagcgcct cgcgcgggat
     6901 tttcttgttg cgcgtggtga acagggcaga gcgggccgtg tcgtttggca tcgctcgcat
     6961 cgtgtccggc cacggcgcaa tatcgaacaa ggaaagctgc atttccttga tctgctgctt
     7021 cgtgtgtttc agcaacgcgg cctgcttggc ctcgctgacc tgttttgcca ggtcctcgcc
     7081 ggcggttttt cgcttcttgg tcgtcatagt tcctcgcgtg tcgatggtca tcgacttcgc
     7141 caaacctgcc gcctcctgtt caagacgacg cgaacgctcc acggcggccg atggcgcggg
     7201 cagggcaggg ggagccagtt gcacgctgtc gcgctcgatc ttggccgtag cttgctggac
     7261 catcgagccg acggactgga aggtttcgcg gggcgcacgc atgacggtgc ggcttgcgat
     7321 ggtttcggca tcctcggcgg aaaaccccgc gtcgatcagt tcttgcctgt atgccttccg
     7381 gtcaaacgtc cgattcattc accctccttg cgggattgcc ccgactcacg ccggggcaat
     7441 gtgcccttat tcctgatttg acccgcctgg tgccttggtg tccagataat ccaccttatc
     7501 ggcaatgaag tcggtcccgt agaccgtctg gccgtccttc tcgtacttgg tattccgaat
     7561 cttgccctgc acgaatacca gcgacccctt gcccaaatac ttgccgtggg cctcggcctg
     7621 agagccaaaa cacttgatgc ggaagaagtc ggtgcgctcc tgcttgtcgc cggcatcgtt
     7681 gcgccacatc taggatctgc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     7741 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     7801 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     7861 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     7921 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     7981 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     8041 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     8101 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     8161 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     8221 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     8281 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     8341 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     8401 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     8461 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     8521 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     8581 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaacca
     8641 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     8701 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     8761 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     8821 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     8881 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     8941 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     9001 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     9061 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     9121 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     9181 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     9241 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     9301 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     9361 ctttttcaat attattgaag catttatcaa ggttattgtc tcatgagcgg atacatattt
     9421 gaatgtattt agaaaaataa acaaataggg gttccgcgca cgaattggcc agcgctgcca
     9481 tttttggggt gaggccgttc gcggccgagg ggcgcagccc ctggggggat gggaggcccg
     9541 cgttagcggg ccgggagggt tcgagaaggg ggggcacccc ccttcggcgt gcgcggtcac
     9601 gcgcacaggg cgcagccctg gttaaaaaca aggtttataa atattggttt aaaagcaggt
     9661 taaaagacag gttagcggtg gccgaaaaac gggcggaaac ccttgcaaat gctggatttt
     9721 ctgcctgtgg acagcccctc aaatgtcaat aggtgcgccc ctcatctgtc agcactctgc
     9781 ccctcaagtg tcaaggatcg cgcccctcat ctgtcagtag tcgcgcccct caagtgtcaa
     9841 taccgcaggg cacttatccc caggcttgtc cacatcatct gtgggaaact cgcgtaaaat
     9901 caggcgtttt cgccgatttg cgaggctggc cagctccacg tcgccggccg aaatcgagcc
     9961 tgcccctcat ctgtcaacgc cgcgccgggt gagtcggccc ctcaagtgtc aacgtccgcc
    10021 cctcatctgt cagtgagggc caagttttcc gcgaggtatc cacaacgccg gcggccgcgg
    10081 tgtctcgcac acggcttcga cggcgtttct ggcgcgtttg cagggccata gacggccgcc
    10141 agcccagcgg cgagggcaac cagcccggtg agcgtcgcaa aggagatcct gatctgactg
    10201 atgggctgcc tgtatcgagt ggtgattttg tgccgagctg ccggtcgggg agctgttggc
    10261 tggctggtgg caggatatat tgtggtgtaa acaaattgac gcttagacaa cttaataaca
    10321 cattgcggac gtttttaatg tactggggtg gatgcagtgg gccccac
//
