LOCUS       sequence-271532-        6346 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Vimentin labeling with near-infrared fluorescent protein miRFP713.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6346)
  AUTHORS   Matlashov ME, Shcherbakova DM, Alvelid J, Baloban M, Pennacchietti 
            F, Shemetov AA, Testa I, Verkhusha VV
  TITLE     A set of monomeric near-infrared fluorescent proteins for multicolor
            imaging across scales.
  JOURNAL   Nat Commun. 2020 Jan 13;11(1):239. doi: 10.1038/s41467-019-13897-6.
  PUBMED    31932632
REFERENCE   2  (bases 1 to 6346)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-01-13"; volume: "11(1):239. doi"; pages: " 
            10.1038/s41467-019-13897-6"
COMMENT     Sequence Label: pvimentin-miRFP713
FEATURES             Location/Qualifiers
     source          1..6346
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        73..376
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        377..580
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     531..551
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             2077..3024
                     /codon_start=1
                     /product="phytochrome-based near-infrared fluorescent 
                     protein, originally termed iRFP (Filonov et al., 2011)"
                     /label=iRFP713
                     /note="name was changed to iRFP713 (Shcherbakova and 
                     Verkhusha, 2013)"
                     /translation="AEGSVARQPDLLTCDDEPIHIPGAIQPHGLLLALAADMTIVAGSD
                     NLPELTGLAIGALIGRSAADVFDSETHNRLTIALAEPGAAVGAPITVGFTMRKDAGFIG
                     SWHRHDQLIFLELEPPQRDVAEPQAFFRRTNSAIRRLQAAETLESACAAAAQEVRKITG
                     FDRVMIYRFASDFSGEVIAEDRCAEVESKLGLHYPASTVPAQARRLYTINPVRIIPDIN
                     YRPVPVTPDLNPVTGRPIDLSFAILRSVSPVHLEFMRNIGMHGTMSISILRGERLWGLI
                     VCHHRTPYYVDLDGRQACKRVAERLATQIGVMEE"
     polyA_signal    3146..3267
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3183..3202)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3237..3256
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3274..3729)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3411..3432)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3623..3642
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3756..3860
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3862..4219
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3862..4053
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3885..3905)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4070..4205
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4254..5048
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4254..5048
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4308..4327)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4918..4937
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5236..5255)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5280..5327
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5656..6244
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6145..6164
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 accgccatgc attagttatt aatagtaatc aattacgggg tcattagttc atagcccata
       61 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
      121 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
      181 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
      241 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
      301 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
      361 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt
      421 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca
      481 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg
      541 cggtaggcgt gtacggtggg aggtctatat aagcagagct ggtttagtga accgtcagat
      601 ccgctagcgc taccggactc agatctcgag ctcaagcttc gaattcccat gtccaccagg
      661 tccgtgtcct cgtcctccta ccgcaggatg ttcggcggcc cgggcaccgc gagccggccg
      721 agctccagcc ggagctacgt gactacgtcc acccgcacct acagcctggg cagcgcgctg
      781 cgccccagca ccagccgcag cctctacgcc tcgtccccgg gcggcgtgta tgccacgcgc
      841 tcctctgccg tgcgcctgcg gagcagcgtg cccggggtgc ggctcctgca ggactcggtg
      901 gacttctcgc tggccgacgc catcaacacc gagttcaaga acacccgcac caacgagaag
      961 gtggagctgc aggagctgaa tgaccgcttc gccaactaca tcgacaaggt gcgcttcctg
     1021 gagcagcaga ataagatcct gctggccgag ctcgagcagc tcaagggcca aggcaagtcg
     1081 cgcctggggg acctctacga ggaggagatg cgggagctgc gccggcaggt ggaccagcta
     1141 accaacgaca aagcccgcgt cgaggtggag cgcgacaacc tggccgagga catcatgcgc
     1201 ctccgggaga aattgcagga ggagatgctt cagagagagg aagccgaaaa caccctgcaa
     1261 tctttcagac aggatgttga caatgcgtct ctggcacgtc ttgaccttga acgcaaagtg
     1321 gaatctttgc aagaagagat tgcctttttg aagaaactcc acgaagagga aatccaggag
     1381 ctgcaggctc agattcagga acagcatgtc caaatcgatg tggatgtttc caagcctgac
     1441 ctcacggctg ccctgcgtga cgtacgtcag caatatgaaa gtgtggctgc caagaacctg
     1501 caggaggcag aagaatggta caaatccaag tttgctgacc tctctgaggc tgccaaccgg
     1561 aacaatgacg ccctgcgcca ggcaaagcag gagtccactg agtaccggag acaggtgcag
     1621 tccctcacct gtgaagtgga tgcccttaaa ggaaccaatg agtccctgga acgccagatg
     1681 cgtgaaatgg aagagaactt tgccgttgaa gctgctaact accaagacac tattggccgc
     1741 ctgcaggatg agattcagaa tatgaaggag gaaatggctc gtcaccttcg tgaataccaa
     1801 gacctgctca atgttaagat ggcccttgac attgagattg ccacctacag gaagctgctg
     1861 gaaggcgagg agagcaggat ttctctgcct cttccaaact tttcctccct gaacctgagg
     1921 gaaactaatc tggattcact ccctctggtt gatacccact caaaaaggac acttctgatt
     1981 aagacggttg aaactagaga tggacaggtt atcaacgaaa cttctcagca tcacgatgac
     2041 cttgaagggg atccaccggt cggtggcggt ggcagcgcgg aaggctccgt cgccaggcag
     2101 cctgacctct tgacctgcga cgatgagccg atccatatcc ccggtgccat ccaaccgcat
     2161 ggactgctgc tcgccctcgc cgccgacatg acgatcgttg ccggcagcga caaccttccc
     2221 gaactcaccg gactggcgat cggcgccctg atcggccgct ctgcggccga tgtcttcgac
     2281 tcggagacgc acaaccgtct gacgatcgcc ttggccgagc ccggggcggc cgtcggagca
     2341 ccgatcactg tcggcttcac gatgcgaaag gacgcaggct tcatcggctc ctggcatcgc
     2401 catgatcagc tcatcttcct cgagctcgag cctccccagc gggacgtcgc cgagccgcag
     2461 gcgttcttcc gccgcaccaa cagcgccatc cgccgcctgc aggccgccga aaccttggaa
     2521 agcgcctgcg ccgccgcggc gcaagaggtg cggaagatta ccggcttcga tcgggtgatg
     2581 atctatcgct tcgcctccga cttcagcggc gaagtgatcg cagaggatcg gtgcgccgag
     2641 gtcgagtcaa aactaggcct gcactatcct gcctcaaccg tgccggcgca ggcccgtcgg
     2701 ctctatacca tcaacccggt acggatcatt cccgatatca attatcggcc ggtgccggtc
     2761 accccagacc tcaatccggt caccgggcgg ccgattgatc ttagcttcgc catcctgcgc
     2821 agcgtctcgc ccgtccatct ggagttcatg cgcaacatag gcatgcacgg cacgatgtcg
     2881 atctcgattt tgcgcggcga gcgactgtgg ggattgatcg tttgccatca ccgaacgccg
     2941 tactacgtcg atctcgatgg ccgccaagcc tgcaagaggg tcgccgagag gctggccact
     3001 cagatcggcg tgatggaaga gtgagcggcc gcgactctag atcataatca gccataccac
     3061 atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca
     3121 taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata
     3181 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     3241 tttgtccaaa ctcatcaatg tatcttaagg cgtaaattgt aagcgttaat attttgttaa
     3301 aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca
     3361 aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga
     3421 acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc
     3481 agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc
     3541 gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc
     3601 cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg
     3661 caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac
     3721 agggcgcgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     3781 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     3841 aatattgaaa aaggaagagt cctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt
     3901 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     3961 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     4021 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
     4081 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     4141 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
     4201 aggcctaggc ttttgcaaag atcgatcaag agacaggatg aggatcgttt cgcatgattg
     4261 aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg
     4321 actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg
     4381 ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg
     4441 aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg
     4501 ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc
     4561 tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc
     4621 tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc
     4681 gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc
     4741 aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gagcatgccc gacggcgagg
     4801 atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct
     4861 tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt
     4921 tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc
     4981 tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt
     5041 tcttctgagc gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc
     5101 acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg
     5161 ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc
     5221 tagggggagg ctaactgaaa cacggaagga gacaataccg gaaggaaccc gcgctatgac
     5281 ggcaataaaa agacagaata aaacgcacgg tgttgggtcg tttgttcata aacgcggggt
     5341 tcggtcccag ggctggcact ctgtcgatac cccaccgaga ccccattggg gccaatacgc
     5401 ccgcgtttct tccttttccc caccccaccc cccaagttcg ggtgaaggcc cagggctcgc
     5461 agccaacgtc ggggcggcag gccctgccat agcctcaggt tactcatata tactttagat
     5521 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     5581 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     5641 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     5701 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     5761 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     5821 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     5881 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     5941 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     6001 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     6061 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     6121 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     6181 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     6241 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     6301 catgttcttt cctgcgttat cccctgattc tgtggataac cgtatt
//
