LOCUS       sequence-271537-        6322 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Vimentin labeling with near-infrared fluorescent protein emiRFP670 
            (enhanced miRFP670).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6322)
  AUTHORS   Matlashov ME, Shcherbakova DM, Alvelid J, Baloban M, Pennacchietti 
            F, Shemetov AA, Testa I, Verkhusha VV
  TITLE     A set of monomeric near-infrared fluorescent proteins for multicolor
            imaging across scales.
  JOURNAL   Nat Commun. 2020 Jan 13;11(1):239. doi: 10.1038/s41467-019-13897-6.
  PUBMED    31932632
REFERENCE   2  (bases 1 to 6322)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-01-13"; volume: "11(1):239. doi"; pages: " 
            10.1038/s41467-019-13897-6"
COMMENT     Sequence Label: pvimentin-emiRFP670
FEATURES             Location/Qualifiers
     source          1..6322
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        76..379
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        380..583
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     534..554
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      2065..2074
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    3125..3246
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3162..3181)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3216..3235
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3253..3708)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3390..3411)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3602..3621
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3735..3839
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3841..4198
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3841..4032
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3864..3884)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4049..4184
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4233..5027
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4233..5027
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4287..4306)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4897..4916
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5215..5234)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5259..5306
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5635..6223
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6124..6143
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 attaccgcca tgcattagtt attaatagta atcaattacg gggtcattag ttcatagccc
       61 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
      121 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
      181 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
      241 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
      301 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
      361 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg
      421 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
      481 gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat
      541 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctggtttag tgaaccgtca
      601 gatccgctag cgctaccgga ctcagatctc gagctcaagc ttcgaattcc catgtccacc
      661 aggtccgtgt cctcgtcctc ctaccgcagg atgttcggcg gcccgggcac cgcgagccgg
      721 ccgagctcca gccggagcta cgtgactacg tccacccgca cctacagcct gggcagcgcg
      781 ctgcgcccca gcaccagccg cagcctctac gcctcgtccc cgggcggcgt gtatgccacg
      841 cgctcctctg ccgtgcgcct gcggagcagc gtgcccgggg tgcggctcct gcaggactcg
      901 gtggacttct cgctggccga cgccatcaac accgagttca agaacacccg caccaacgag
      961 aaggtggagc tgcaggagct gaatgaccgc ttcgccaact acatcgacaa ggtgcgcttc
     1021 ctggagcagc agaataagat cctgctggcc gagctcgagc agctcaaggg ccaaggcaag
     1081 tcgcgcctgg gggacctcta cgaggaggag atgcgggagc tgcgccggca ggtggaccag
     1141 ctaaccaacg acaaagcccg cgtcgaggtg gagcgcgaca acctggccga ggacatcatg
     1201 cgcctccggg agaaattgca ggaggagatg cttcagagag aggaagccga aaacaccctg
     1261 caatctttca gacaggatgt tgacaatgcg tctctggcac gtcttgacct tgaacgcaaa
     1321 gtggaatctt tgcaagaaga gattgccttt ttgaagaaac tccacgaaga ggaaatccag
     1381 gagctgcagg ctcagattca ggaacagcat gtccaaatcg atgtggatgt ttccaagcct
     1441 gacctcacgg ctgccctgcg tgacgtacgt cagcaatatg aaagtgtggc tgccaagaac
     1501 ctgcaggagg cagaagaatg gtacaaatcc aagtttgctg acctctctga ggctgccaac
     1561 cggaacaatg acgccctgcg ccaggcaaag caggagtcca ctgagtaccg gagacaggtg
     1621 cagtccctca cctgtgaagt ggatgccctt aaaggaacca atgagtccct ggaacgccag
     1681 atgcgtgaaa tggaagagaa ctttgccgtt gaagctgcta actaccaaga cactattggc
     1741 cgcctgcagg atgagattca gaatatgaag gaggaaatgg ctcgtcacct tcgtgaatac
     1801 caagacctgc tcaatgttaa gatggccctt gacattgaga ttgccaccta caggaagctg
     1861 ctggaaggcg aggagagcag gatttctctg cctcttccaa acttttcctc cctgaacctg
     1921 agggaaacta atctggattc actccctctg gttgataccc actcaaaaag gacacttctg
     1981 attaagacgg ttgaaactag agatggacag gttatcaacg aaacttctca gcatcacgat
     2041 gaccttgaag gggatccacc ggtcgccacc atggcggaag gctccgtcgc caggcagcct
     2101 gacctcttga cctgcgaaca tgaagagatc cacctcgccg gctcgatcca gccgcatggc
     2161 gcgcttctgg tcgtcagcga acatgatcat cgcgtcatcc aggccagcgc caacgccgcg
     2221 gaatttctga atctcggaag cgtactcggc gttccgctcg ccgagatcga cggcgatctg
     2281 ttgatcaaga tcctgccgca tctcgatccc accgccgaag gcatgccggt cgcggtgcgc
     2341 tgccggatcg gcaatccctc tacggagtac tgcggtctga tgcatcggcc tccggaaggc
     2401 gggctgatca tcgaactcga acgtgccggc ccgtcgatcg atctgtcagg cacgctggcg
     2461 ccggcgctgg agcggatccg cacggcgggt tcactgcgcg cgctgtgcga tgacaccgtg
     2521 ctgctgtttc agcagtgcac cggctacgac cgggtgatgg tgtatcgttt cgatgagcaa
     2581 ggccacggcc tggtattctc cgagtgccat gtgcctgggc tcgaatccta tttcggcaac
     2641 cgctatccgt cgtcgactgt cccgcagatg gcgcggcagc tgtacgtgcg gcagcgcgtc
     2701 cgcgtgctgg tcgacgtcac ctatcagccg gtgccgctgg agccgcggct gtcgccgctg
     2761 accgggcgcg atctcgacat gtcgggctgc ttcctgcgct cgatgtcgcc gtgccatctg
     2821 cagttcctga aggacatggg cgtgcgcgcc accctggcgg tgtcgctggt ggtcggcggc
     2881 aagctgtggg gcctggttgt ctgtcaccat tatctgccgc gcttcatccg tttcgagctg
     2941 cgggcgatct gcaaacggct cgccgaaagg atcgcgacgc ggatcaccgc gcttgagagc
     3001 taagcggccg cgactctaga tcataatcag ccataccaca tttgtagagg ttttacttgc
     3061 tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt
     3121 tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     3181 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     3241 atcttaaggc gtaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt
     3301 aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag
     3361 aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga
     3421 acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg
     3481 aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc
     3541 ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg
     3601 aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc
     3661 gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtca ggtggcactt
     3721 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     3781 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagtc
     3841 ctgaggcgga aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg
     3901 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg
     3961 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     4021 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     4081 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
     4141 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaga
     4201 tcgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag
     4261 gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg
     4321 gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca
     4381 agaccgacct gtccggtgcc ctgaatgaac tgcaagacga ggcagcgcgg ctatcgtggc
     4441 tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg
     4501 actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg
     4561 ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta
     4621 cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag
     4681 ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac
     4741 tgttcgccag gctcaaggcg agcatgcccg acggcgagga tctcgtcgtg acccatggcg
     4801 atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg
     4861 gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg
     4921 aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg
     4981 attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg
     5041 gttcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc
     5101 cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct
     5161 ccagcgcggg gatctcatgc tggagttctt cgcccaccct agggggaggc taactgaaac
     5221 acggaaggag acaataccgg aaggaacccg cgctatgacg gcaataaaaa gacagaataa
     5281 aacgcacggt gttgggtcgt ttgttcataa acgcggggtt cggtcccagg gctggcactc
     5341 tgtcgatacc ccaccgagac cccattgggg ccaatacgcc cgcgtttctt ccttttcccc
     5401 accccacccc ccaagttcgg gtgaaggccc agggctcgca gccaacgtcg gggcggcagg
     5461 ccctgccata gcctcaggtt actcatatat actttagatt gatttaaaac ttcattttta
     5521 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     5581 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     5641 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     5701 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     5761 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     5821 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     5881 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     5941 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     6001 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     6061 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     6121 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     6181 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     6241 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     6301 ccctgattct gtggataacc gt
//
