LOCUS       sequence-271539-        6320 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Human alpha-tubulin labeling with a near-infrared fluorescent 
            protein emiRFP670 (enhanced miRFP670).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6320)
  AUTHORS   Matlashov ME, Shcherbakova DM, Alvelid J, Baloban M, Pennacchietti 
            F, Shemetov AA, Testa I, Verkhusha VV
  TITLE     A set of monomeric near-infrared fluorescent proteins for multicolor
            imaging across scales.
  JOURNAL   Nat Commun. 2020 Jan 13;11(1):239. doi: 10.1038/s41467-019-13897-6.
  PUBMED    31932632
REFERENCE   2  (bases 1 to 6320)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-01-13"; volume: "11(1):239. doi"; pages: " 
            10.1038/s41467-019-13897-6"
COMMENT     Sequence Label: pemiRFP670-tubulin
FEATURES             Location/Qualifiers
     source          1..6320
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        79..382
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        383..586
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     537..557
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      615..624
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    3126..3247
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3163..3182)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3217..3236
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3254..3709)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3391..3412)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3603..3622
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3736..3840
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3842..4199
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3842..4033
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3865..3885)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4050..4185
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4234..5028
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4234..5028
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4288..4307)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4898..4917
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5216..5235)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5260..5307
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5636..6224
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6125..6144
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgtattaccg ccatgcatta gttattaata gtaatcaatt acggggtcat tagttcatag
       61 cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
      121 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
      181 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
      241 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
      301 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
      361 attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata
      421 gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt
      481 ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca
      541 aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctggtt tagtgaaccg
      601 tcagatccgc tagcgccacc atggcggaag gctccgtcgc caggcagcct gacctcttga
      661 cctgcgaaca tgaagagatc cacctcgccg gctcgatcca gccgcatggc gcgcttctgg
      721 tcgtcagcga acatgatcat cgcgtcatcc aggccagcgc caacgccgcg gaatttctga
      781 atctcggaag cgtactcggc gttccgctcg ccgagatcga cggcgatctg ttgatcaaga
      841 tcctgccgca tctcgatccc accgccgaag gcatgccggt cgcggtgcgc tgccggatcg
      901 gcaatccctc tacggagtac tgcggtctga tgcatcggcc tccggaaggc gggctgatca
      961 tcgaactcga acgtgccggc ccgtcgatcg atctgtcagg cacgctggcg ccggcgctgg
     1021 agcggatccg cacggcgggt tcactgcgcg cgctgtgcga tgacaccgtg ctgctgtttc
     1081 agcagtgcac cggctacgac cgggtgatgg tgtatcgttt cgatgagcaa ggccacggcc
     1141 tggtattctc cgagtgccat gtgcctgggc tcgaatccta tttcggcaac cgctatccgt
     1201 cgtcgactgt cccgcagatg gcgcggcagc tgtacgtgcg gcagcgcgtc cgcgtgctgg
     1261 tcgacgtcac ctatcagccg gtgccgctgg agccgcggct gtcgccgctg accgggcgcg
     1321 atctcgacat gtcgggctgc ttcctgcgct cgatgtcgcc gtgccatctg cagttcctga
     1381 aggacatggg cgtgcgcgcc accctggcgg tgtcgctggt ggtcggcggc aagctgtggg
     1441 gcctggttgt ctgtcaccat tatctgccgc gcttcatccg tttcgagctg cgggcgatct
     1501 gcaaacggct cgccgaaagg atcgcgacgc ggatcaccgc gcttgagagc ggaggtggtg
     1561 gatcaggtgg aggaggttct ggaggtggtg gatcaggagg aggtggttct ggaggtggtg
     1621 gatcaggagg aagatctcga gtgcgtgagt gcatctccat ccacgttggc caggctggtg
     1681 tccagattgg caatgcctgc tgggagctct actgcctgga acacggcatc cagcccgatg
     1741 gccagatgcc aagtgacaag accattgggg gaggagatga ctccttcaac accttcttca
     1801 gtgagacggg cgctggcaag cacgtgcccc gggctgtgtt tgtagacttg gaacccacag
     1861 tcattgatga agttcgcact ggcacctacc gccagctctt ccaccctgag cagctcatca
     1921 caggcaagga agatgctgcc aataactatg cccgagggca ctacaccatt ggcaaggaga
     1981 tcattgacct tgtgttggac cgaattcgca agctggctga ccagtgcacc ggtcttcagg
     2041 gcttcttggt tttccacagc tttggtgggg gaactggttc tgggttcacc tccctgctca
     2101 tggaacgtct ctcagttgat tatggcaaga agtccaagct ggagttctcc atttacccag
     2161 caccccaggt ttccacagct gtagttgagc cctacaactc catcctcacc acccacacca
     2221 ccctggagca ctctgattgt gccttcatgg tagacaatga ggccatctat gacatctgtc
     2281 gtagaaacct cgatatcgag cgcccaacct acactaacct taaccgcctt attagccaga
     2341 ttgtgtcctc catcactgct tccctgagat ttgatggagc cctgaatgtt gacctgacag
     2401 aattccagac caacctggtg ccctaccccc gcatccactt ccctctggcc acatatgccc
     2461 ctgtcatctc tgctgagaaa gcctaccatg aacagctttc tgtagcagag atcaccaatg
     2521 cttgctttga gccagccaac cagatggtga aatgtgaccc tcgccatggt aaatacatgg
     2581 cttgctgcct gttgtaccgt ggtgacgtgg ttcccaaaga tgtcaatgct gccattgcca
     2641 ccatcaaaac caagcgcagc atccagtttg tggattggtg ccccactggc ttcaaggttg
     2701 gcatcaacta ccagcctccc actgtggtgc ctggtggaga cctggccaag gtacagagag
     2761 ctgtgtgcat gctgagcaac accacagcca ttgctgaggc ctgggctcgc ctggaccaca
     2821 agtttgacct gatgtatgcc aagcgtgcct ttgttcactg gtacgtgggt gaggggatgg
     2881 aggaaggcga gttttcagag gcccgtgaag atatggctgc ccttgagaag gattatgagg
     2941 aggttggtgt ggattctgtt gaaggagagg gtgaggaaga aggagaggaa tactaaggat
     3001 ccaccggatc tagataactg atcataatca gccataccac atttgtagag gttttacttg
     3061 ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg
     3121 ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     3181 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     3241 tatcttaacg cgtaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt
     3301 taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa
     3361 gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag
     3421 aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt
     3481 gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac
     3541 cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag
     3601 gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg
     3661 cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc aggtggcact
     3721 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     3781 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     3841 cctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
     3901 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
     3961 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     4021 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     4081 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
     4141 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaag
     4201 atcgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca
     4261 ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc
     4321 ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc
     4381 aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg gctatcgtgg
     4441 ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg
     4501 gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct
     4561 gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct
     4621 acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa
     4681 gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa
     4741 ctgttcgcca ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt gacccatggc
     4801 gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt
     4861 ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct
     4921 gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc
     4981 gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg
     5041 ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg
     5101 ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc
     5161 tccagcgcgg ggatctcatg ctggagttct tcgcccaccc tagggggagg ctaactgaaa
     5221 cacggaagga gacaataccg gaaggaaccc gcgctatgac ggcaataaaa agacagaata
     5281 aaacgcacgg tgttgggtcg tttgttcata aacgcggggt tcggtcccag ggctggcact
     5341 ctgtcgatac cccaccgaga ccccattggg gccaatacgc ccgcgtttct tccttttccc
     5401 caccccaccc cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc ggggcggcag
     5461 gccctgccat agcctcaggt tactcatata tactttagat tgatttaaaa cttcattttt
     5521 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     5581 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     5641 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     5701 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     5761 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     5821 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     5881 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     5941 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     6001 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     6061 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     6121 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     6181 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     6241 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     6301 cccctgattc tgtggataac
//
