LOCUS       sequence-271554-         615 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR-Cas9 library validation (positive control).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 615)
  AUTHORS   Arnan C, Ullrich S, Pulido-Quetglas C, Nurtdinov R, Esteban A, 
            Blanco-Fernandez J, Aparicio-Prat E, Johnson R, Perez-Lluch S, Guigo
            R
  TITLE     Paired guide RNA CRISPR-Cas9 screening for protein-coding genes and 
            lncRNAs involved in transdifferentiation of human B-cells to 
            macrophages.
  JOURNAL   BMC Genomics. 2022 May 26;23(1):402. doi: 
            10.1186/s12864-022-08612-7.
  PUBMED    35619054
REFERENCE   2  (bases 1 to 615)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Genomics."; date: "2022-05-26"; volume: "23(1):402. doi"; pages: " 
            10.1186/s12864-022-08612-7"
COMMENT     Sequence Label: pDECKO_mCherry_SPI1
FEATURES             Location/Qualifiers
     source          1..615
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     44..63
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        144..219
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        226..440
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     361..380
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     misc_RNA        469..544
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 ganatnctgg gtagtttgca gttttaaatt atgttttaaa atggactatc atatgcttac
       61 cgtaacttga aagtatttcg atttcttggc tttatatatc ttgtggaaag gacgaaacac
      121 cggactcgct gtgcacgtgg tgggttttag agctagaaat agcaagttaa aataaggcta
      181 gtccgttatc aacttgaaaa agtggcaccg agtcggtgct tttttgaacg ctgacgtcat
      241 caacccgctc caaggaatcg cgggcccagt gtcactaggc gggaacaccc agcgcgcgtg
      301 cgccctggca ggaagatggc tgtgagggac aggggagtgg cgccctgcaa tatttgcatg
      361 tcgctatgtg ttctgggaaa tcaccataaa cgtgaaatgt ctttggattt gggagtctta
      421 taagttctgt atgagaccac tctttccctg ggtactggag gcacatccgt tttagagcta
      481 gaaatagcaa gttaaaataa ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg
      541 gtgctttttt aagcttggcg taactagatc ttgagacaaa tggcagtatt catccacaat
      601 tttaaaagaa aaggg
//
