LOCUS       sequence-271559-        3277 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Golden Gate Level 0 S module for N-terminal fluorescent protein 
            tagging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3277)
  AUTHORS   Luginbuehl LH, El-Sharnouby S, Wang N, Hibberd JM
  TITLE     Fluorescent reporters for functional analysis in rice leaves.
  JOURNAL   Plant Direct. 2020 Feb 11;4(2):e00188. doi: 10.1002/pld3.188. 
            eCollection 2020 Feb.
  PUBMED    32072132
REFERENCE   2  (bases 1 to 3277)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Direct."; date: "2020-02-11"; volume: "4(2):e00188. doi"; pages: " 
            10.1002/pld3.188. eCollection 2020 Feb"
COMMENT     Sequence Label: pL0M-S-mTurquoise2-EC18152
FEATURES             Location/Qualifiers
     source          1..3277
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        81..185
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      1370..1958
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1859..1878
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2051..2068
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(2996..3015)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2997..3015)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3026..3043)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3026..3042)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3035..3057)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaggtg
       61 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
      121 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
      181 agagtatgcg tagccgtaat tggagccgta ccctgaccga acgtagcggc ggtaatggtg
      241 cggtggcggt gtttatggcg tgctatgatt gcttttttgg cgtgcagagc atgccgcgtg
      301 cgagcaaaca gcaggcgcgt tatgcggtgg gccgttgcct gatgctgtgg agcagcaacg
      361 atgtgaccca gcagggcagc cgtccgaaaa ccaaactgaa cattatgcgt gaagcggtga
      421 ttgcggaagt gagcacccag ctgtctgaag tggtgggcgt gattgaacgt catctggaac
      481 cgaccctgct ggccgtgcat ctgtatggca gcgcggtgga tggcggcctg aaaccgcata
      541 gcgatattga tctgctggtg accgtgaccg tgcgtctgga tgaaaccacc cgtcgtgcgc
      601 tgattaacga tctgctggaa accagcgcga gcccgggtga aagcgaaatt ctgcgtgcgg
      661 tggaagtgac catcgtggtg catgatgaca ttattccgtg gcgttatccg gcgaaacgtg
      721 agctgcagtt tggcgaatgg cagcgtaacg atattctggc cggcattttt gaaccggcga
      781 ccattgatat cgatctggcc attctgctga ccaaagcgcg tgaacatagc gtggcgctgg
      841 ttggtccggc agcggaagaa ctgtttgatc cggtgccgga acaggacctg tttgaagcgc
      901 tgaacgaaac cctgaccctg tggaacagcc cgccggattg ggcgggtgat gaacgcaacg
      961 tggtgctgac cctgagccgt atttggtata gcgcggtgac cggcaaaatt gcgccgaaag
     1021 atgtggcggc ggattgggcc atggaacgtc tgccggcgca gtatcagccg gtgattctgg
     1081 aagcgcgtca ggcgtatctg ggccaggaag aagatcgtct ggccagccgt gcggatcagc
     1141 tggaagaatt tgtgcattac gtgaaaggcg aaattaccaa agtggtgggt aaataataac
     1201 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     1261 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     1321 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     1381 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     1441 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     1501 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     1561 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     1621 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     1681 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     1741 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     1801 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     1861 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     1921 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     1981 tacggttcct ggccttttgc tggccttttg ctcattaggc accccaggct ttacccgaac
     2041 gaccgagcgc agcgagtcag tgagcgagga agcggagagc gcccaatacg caaggaaaca
     2101 gctatgacca tgttaatgca gctggcacga caggtttccc gactggaaag cgggcagtga
     2161 agtgaaggcc catgaggcca gttaattaag aggtacccac ttcgtggtct caacctcctt
     2221 tatacagctc gtccataccc aaagttatcc ctgccgcggt cacaaactcc agcagaacca
     2281 tgtgatctct tttttcattc ggatccttac taagcttcga ttgagttgac aagtaatggt
     2341 tatccggcaa caagacaggt ccgtcgccga taggtgtgtt ctgttgataa tggtccgcca
     2401 gttgaacacc accgtcctcg atattgtgcc ggatcttaaa gttcgctttg ataccgttct
     2461 tttgcttgtc ggcagtgata taaacgttgt cgctaaagta gttgtactcc aacttatgac
     2521 ccaaaatgtt cccgtcctcc ttgaagtcga ttcccttgag ttctatgcga ttaacgaggg
     2581 tgtcgccctc gaacttgact tcggcgcgtg ttttatagtt cccatcatcc ttgaaaaata
     2641 tggttctttc ttggacgtag ccttcaggca tagcactctt gaagaaatcg tgctgtttca
     2701 tgtgatcggg ataccgggca aagcactgca ccccccatga cagtgttgta accagcgtgg
     2761 gccaggggac cggcagtttg ccggtagtgc agataaattt aagggtcagc ttaccatagg
     2821 tcgcatctcc ctctccttcc ccagacacgg agaacttatg tccattgaca tccccatcaa
     2881 gctccacgag tattggcacg acgcccgtga acagctcttc ccctttggac gccatttgag
     2941 accacagagt ggagctcatg gcgcgcctag gccttgacgg ccttcactca attcgcccta
     3001 tagtgagtcg tattacgtcg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa
     3061 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
     3121 atagcgaaga ggcccgcacc gaaacgccct tcccaacagt tgcgcagcct gaatggcgaa
     3181 tgggagcgcc ctgtagcggc cactcaaccc tatctcggtc tattcttttg atttataagg
     3241 gattttgccg atttcggcct attggttaaa aaatgag
//
