LOCUS       sequence-27156-e         575 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 575)
  AUTHORS   Tontonoz P, Hu E, Spiegelman BM
  TITLE     Stimulation of adipogenesis in fibroblasts by PPAR gamma 2, a 
            lipid-activated transcription factor.
  JOURNAL   Cell. 1994 Dec 30. 79(7):1147-56.
  PUBMED    8001151
REFERENCE   2  (bases 1 to 575)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 1994 
            Dec 30. 79(7):1147-56."
COMMENT     Sequence Label: pBABE puro PPAR gamma 2
FEATURES             Location/Qualifiers
     source          1..575
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             23..46
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             59..85
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             59..85
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 actcgacgct cgatgaacca tggactacaa ggacgacgat gacaagctcg atggaggata
       61 cccctacgac gtgcccgact acgccggagg actcgagatg ggtgaaactc tgggagattc
      121 tcctgttgac ccagagcatg gtgccttcgc tgatgcactg cctatgagca cttcacaaga
      181 aattaccatg gttgacacag agatgccatt ctggcccacc aacttcggaa tcagctctgt
      241 ggacctctcc gtgatggaag accactcgca ttcctttgac atcaagccct ttaccacagt
      301 tgatttctcc agcatttctg ctccacacta tgaagacatt ccattcacaa gagctgaccc
      361 aatggttgct gattacaaat atgacctgaa gctccaagaa taccaaagtg cgatcaaagt
      421 agaacctgca tctccacctt attattctga aaagacccag ctctacaaca ggcctcatga
      481 agaaccttct aactccctca tggccattga gtgccgagtc tgtggggata aagcatcagg
      541 cttccactat ggagttcatg cttgtgaagg atgca
//
