LOCUS       sequence-271561-        3272 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Golden Gate Level 0 S module for N-terminal fluorescent protein 
            tagging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3272)
  AUTHORS   Luginbuehl LH, El-Sharnouby S, Wang N, Hibberd JM
  TITLE     Fluorescent reporters for functional analysis in rice leaves.
  JOURNAL   Plant Direct. 2020 Feb 11;4(2):e00188. doi: 10.1002/pld3.188. 
            eCollection 2020 Feb.
  PUBMED    32072132
REFERENCE   2  (bases 1 to 3272)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Direct."; date: "2020-02-11"; volume: "4(2):e00188. doi"; pages: " 
            10.1002/pld3.188. eCollection 2020 Feb"
COMMENT     Sequence Label: pL0M-S-mNeonGreen-EC18153
FEATURES             Location/Qualifiers
     source          1..3272
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     267..289
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     281..298
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     282..298
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     309..328
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        309..327
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1251..1268)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1361..1949)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1441..1460)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        complement(3134..3238)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc
       61 aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga
      121 gtggccgcta cagggcgctc ccattcgcca ttcaggctgc gcaactgttg ggaagggcgt
      181 ttcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg
      241 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga
      301 gcgcgacgta atacgactca ctatagggcg aattgagtga aggccgtcaa ggccacgtgt
      361 cttgtccaga gctccactct gtggtctcaa atggcgtcga agggcgaaga ggataatatg
      421 gcctcgctcc cggcgactca cgaattgcat atattcgggt cgattaacgg ggtcgacttc
      481 gatatggtgg gccaaggtac agggaatccg aacgatggat atgaagaact caacctgaag
      541 tcgactaagg gagatctcca gttttcgcct tggatactgg tcccgcatat aggctacgga
      601 ttccatcagt atttgccgta cccagacgga atgtccccgt ttcaggcagc catggttgat
      661 ggatctggat accaagtgca tcgcactatg cagtttgagg atggtgccag tcttacggtt
      721 aattatcggt acacatacga agggtcccac attaagggcg aggcccaagt taaaggtacg
      781 ggattcccag ccgacgggcc cgtgatgacc aattcgctta ctgcagctga ttggtgtcgg
      841 agcaaaaaga cgtaccccaa tgataaaact atcatctcaa ctttcaaatg gtcatatacc
      901 acaggaaacg gtaagagata taggtctacg gccaggacta catatacgtt cgcaaaacct
      961 atggcagcga actacttgaa gaatcaacca atgtacgtct ttcgcaaaac ggagctgaaa
     1021 cactctaaga cagaattgaa cttcaaagaa tggcaaaaag cgttcacgga cgtgatggga
     1081 atggatgaac tctataaagg aggttgagac cacgaagtgg gtacctggag cacaagactg
     1141 gcctcatggg ccttcacttc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     1201 cattaacatg gtcatagctg tttccttgcg tattgggcgc tctccgcttc ctcgctcact
     1261 gactcgctgc gctcggtcgt tcgggtaaag cctggggtgc ctaatgagca aaaggccagc
     1321 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     1381 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     1441 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     1501 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     1561 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     1621 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     1681 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     1741 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     1801 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     1861 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     1921 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     1981 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     2041 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     2101 agtaaacttg gtctgacagt tattatttac ccaccacttt ggtaatttcg cctttcacgt
     2161 aatgcacaaa ttcttccagc tgatccgcac ggctggccag acgatcttct tcctggccca
     2221 gatacgcctg acgcgcttcc agaatcaccg gctgatactg cgccggcaga cgttccatgg
     2281 cccaatccgc cgccacatct ttcggcgcaa ttttgccggt caccgcgcta taccaaatac
     2341 ggctcagggt cagcaccacg ttgcgttcat cacccgccca atccggcggg ctgttccaca
     2401 gggtcagggt ttcgttcagc gcttcaaaca ggtcctgttc cggcaccgga tcaaacagtt
     2461 cttccgctgc cggaccaacc agcgccacgc tatgttcacg cgctttggtc agcagaatgg
     2521 ccagatcgat atcaatggtc gccggttcaa aaatgccggc cagaatatcg ttacgctgcc
     2581 attcgccaaa ctgcagctca cgtttcgccg gataacgcca cggaataatg tcatcatgca
     2641 ccacgatggt cacttccacc gcacgcagaa tttcgctttc acccgggctc gcgctggttt
     2701 ccagcagatc gttaatcagc gcacgacggg tggtttcatc cagacgcacg gtcacggtca
     2761 ccagcagatc aatatcgcta tgcggtttca ggccgccatc caccgcgctg ccatacagat
     2821 gcacggccag cagggtcggt tccagatgac gttcaatcac gcccaccact tcagacagct
     2881 gggtgctcac ttccgcaatc accgcttcac gcataatgtt cagtttggtt ttcggacggc
     2941 tgccctgctg ggtcacatcg ttgctgctcc acagcatcag gcaacggccc accgcataac
     3001 gcgcctgctg tttgctcgca cgcggcatgc tctgcacgcc aaaaaagcaa tcatagcacg
     3061 ccataaacac cgccaccgca ccattaccgc cgctacgttc ggtcagggta cggctccaat
     3121 tacggctacg catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     3181 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     3241 catttccccg aaaagtgcca cctaaattgt aa
//
