LOCUS       sequence-271562-        3218 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Golden Gate Level 0 S module for N-terminal fluorescent protein 
            tagging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3218)
  AUTHORS   Luginbuehl LH, El-Sharnouby S, Wang N, Hibberd JM
  TITLE     Fluorescent reporters for functional analysis in rice leaves.
  JOURNAL   Plant Direct. 2020 Feb 11;4(2):e00188. doi: 10.1002/pld3.188. 
            eCollection 2020 Feb.
  PUBMED    32072132
REFERENCE   2  (bases 1 to 3218)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Direct."; date: "2020-02-11"; volume: "4(2):e00188. doi"; pages: " 
            10.1002/pld3.188. eCollection 2020 Feb"
COMMENT     Sequence Label: pL0M-S-mKO-kappa-EC18154
FEATURES             Location/Qualifiers
     source          1..3218
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(66..170)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     471..493
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     485..502
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     486..502
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     513..532
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        513..531
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1401..1418)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1511..2099)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1591..1610)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 accgccaccg caccattacc gccgctacgt tcggtcaggg tacggctcca attacggcta
       61 cgcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
      121 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
      181 cgaaaagtgc cacctaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt
      241 gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa
      301 aagaatagac cgagataggg ttgagtggcc gctacagggc gctcccattc gccattcagg
      361 ctgcgcaact gttgggaagg gcgtttcggt gcgggcctct tcgctattac gccagctggc
      421 gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg
      481 acgttgtaaa acgacggcca gtgagcgcga cgtaatacga ctcactatag ggcgaattga
      541 gtgaaggccg tcaaggccac gtgtcttgtc cagagctcca ctctgtggtc tcaaatggcg
      601 tctgttatca agcccgagat gaaaatgcgg tactacatgg atggctctgt caatggtcac
      661 gaatttacta tcgagggaga aggtacaggg cggccatacg aagggcatca agagatgact
      721 cttcgcgtga ctatggccga aggcggacct atgccatttg cattcgattt ggtttcgcac
      781 gtcttttgct atggacatcg cgttttcacg aaataccctg aagaaattcc tgattacttc
      841 aagcaagcct ttcccgaagg gctgagctgg gaaagatcat tggagttcga agatgggggt
      901 agtgcaagtg tgagtgccca tataagcttg cgcggcaata cattctatca caagtcgaag
      961 ttcactggag tcaattttcc tgctgatgga cctatcatgc agaaccagtc agtggactgg
     1021 gagccaagta cagaaaaaat cacagcatca gatggagttc tcaaaggcga cgtgaccatg
     1081 tatttgaagc ttgagggtgg gggcaatcat aagtgccagt tcaagactac ttacaaggcg
     1141 gcgaaggaaa tcttggaaat gcctggtgac cactacatcg gtcacagact ggtgagaaaa
     1201 acagagggga acattacaga gcaagttgaa gatgcagtgg cacatagcgg aggttgagac
     1261 cacgaagtgg gtacctggag cacaagactg gcctcatggg ccttcacttc actgcccgct
     1321 ttccagtcgg gaaacctgtc gtgccagctg cattaacatg gtcatagctg tttccttgcg
     1381 tattgggcgc tctccgcttc ctcgctcact gactcgctgc gctcggtcgt tcgggtaaag
     1441 cctggggtgc ctaatgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     1501 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     1561 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     1621 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     1681 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     1741 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     1801 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     1861 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     1921 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     1981 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     2041 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     2101 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     2161 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     2221 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt tattatttac
     2281 ccaccacttt ggtaatttcg cctttcacgt aatgcacaaa ttcttccagc tgatccgcac
     2341 ggctggccag acgatcttct tcctggccca gatacgcctg acgcgcttcc agaatcaccg
     2401 gctgatactg cgccggcaga cgttccatgg cccaatccgc cgccacatct ttcggcgcaa
     2461 ttttgccggt caccgcgcta taccaaatac ggctcagggt cagcaccacg ttgcgttcat
     2521 cacccgccca atccggcggg ctgttccaca gggtcagggt ttcgttcagc gcttcaaaca
     2581 ggtcctgttc cggcaccgga tcaaacagtt cttccgctgc cggaccaacc agcgccacgc
     2641 tatgttcacg cgctttggtc agcagaatgg ccagatcgat atcaatggtc gccggttcaa
     2701 aaatgccggc cagaatatcg ttacgctgcc attcgccaaa ctgcagctca cgtttcgccg
     2761 gataacgcca cggaataatg tcatcatgca ccacgatggt cacttccacc gcacgcagaa
     2821 tttcgctttc acccgggctc gcgctggttt ccagcagatc gttaatcagc gcacgacggg
     2881 tggtttcatc cagacgcacg gtcacggtca ccagcagatc aatatcgcta tgcggtttca
     2941 ggccgccatc caccgcgctg ccatacagat gcacggccag cagggtcggt tccagatgac
     3001 gttcaatcac gcccaccact tcagacagct gggtgctcac ttccgcaatc accgcttcac
     3061 gcataatgtt cagtttggtt ttcggacggc tgccctgctg ggtcacatcg ttgctgctcc
     3121 acagcatcag gcaacggccc accgcataac gcgcctgctg tttgctcgca cgcggcatgc
     3181 tctgcacgcc aaaaaagcaa tcatagcacg ccataaac
//
