LOCUS       sequence-271563-        3281 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Golden Gate Level 0 S module for N-terminal fluorescent protein 
            tagging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3281)
  AUTHORS   Luginbuehl LH, El-Sharnouby S, Wang N, Hibberd JM
  TITLE     Fluorescent reporters for functional analysis in rice leaves.
  JOURNAL   Plant Direct. 2020 Feb 11;4(2):e00188. doi: 10.1002/pld3.188. 
            eCollection 2020 Feb.
  PUBMED    32072132
REFERENCE   2  (bases 1 to 3281)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Direct."; date: "2020-02-11"; volume: "4(2):e00188. doi"; pages: " 
            10.1002/pld3.188. eCollection 2020 Feb"
COMMENT     Sequence Label: pL0M-S-mClover3-EC18157
FEATURES             Location/Qualifiers
     source          1..3281
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(44..148)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     449..471
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     463..480
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     464..480
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     491..510
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        491..509
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1442..1459)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1552..2140)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1632..1651)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgctacgttc ggtcagggta cggctccaat tacggctacg catactcttc ctttttcaat
       61 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
      121 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctaaattgt
      181 aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa
      241 ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt
      301 gagtggccgc tacagggcgc tcccattcgc cattcaggct gcgcaactgt tgggaagggc
      361 gtttcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg
      421 cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt
      481 gagcgcgacg taatacgact cactataggg cgaattgagt gaaggccgtc aaggccacgt
      541 gtcttgtcca gagctccact ctgtggtctc aaatggcgtc caagggggaa gaactgttta
      601 ccggagtcgt tcctattctg gtcgaactgg atggtgatgt caatggccac aagttctcgg
      661 tccggggtga aggtgagggt gacgcaacaa acggcaagct gacactcaag tttatttgca
      721 caacaggcaa gctgcctgtc ccgtggccaa cacttgttac gacattcggt tatggtgtcg
      781 catgtttttc acgctatcct gaccacatga agcagcatga tttcttcaaa tcggccatgc
      841 ccgaaggtta tgttcaggag cggactatat ctttcaagga cgacggaacg tacaaaacgc
      901 gcgccgaagt taagttcgaa ggtgacacct tggtgaatcg gattgagttg aagggaatcg
      961 actttaagga ggacgggaac atacttggac acaagttgga gtacaatttt aattctcatt
     1021 atgtctacat aaccgcggac aagcaaaaga attgtataaa ggcgaatttt aaaataaggc
     1081 ataatgtgga agatggaagc gtgcagcttg ctgatcatta tcaacaaaat acgccgatag
     1141 gagatgggcc agtgttgctg cctgacaacc actacctctc ccatcagagc aaattgtcaa
     1201 aagacccgaa tgaaaaacgc gatcacatgg tcctcctgga gtttgtcacc gcagctggta
     1261 tcacgcacgg catggatgaa ctttacaaag gaggttgaga ccacgaagtg ggtacctgga
     1321 gcacaagact ggcctcatgg gccttcactt cactgcccgc tttccagtcg ggaaacctgt
     1381 cgtgccagct gcattaacat ggtcatagct gtttccttgc gtattgggcg ctctccgctt
     1441 cctcgctcac tgactcgctg cgctcggtcg ttcgggtaaa gcctggggtg cctaatgagc
     1501 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     1561 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     1621 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     1681 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     1741 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     1801 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     1861 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     1921 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     1981 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     2041 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     2101 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     2161 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     2221 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     2281 aagtatatat gagtaaactt ggtctgacag ttattattta cccaccactt tggtaatttc
     2341 gcctttcacg taatgcacaa attcttccag ctgatccgca cggctggcca gacgatcttc
     2401 ttcctggccc agatacgcct gacgcgcttc cagaatcacc ggctgatact gcgccggcag
     2461 acgttccatg gcccaatccg ccgccacatc tttcggcgca attttgccgg tcaccgcgct
     2521 ataccaaata cggctcaggg tcagcaccac gttgcgttca tcacccgccc aatccggcgg
     2581 gctgttccac agggtcaggg tttcgttcag cgcttcaaac aggtcctgtt ccggcaccgg
     2641 atcaaacagt tcttccgctg ccggaccaac cagcgccacg ctatgttcac gcgctttggt
     2701 cagcagaatg gccagatcga tatcaatggt cgccggttca aaaatgccgg ccagaatatc
     2761 gttacgctgc cattcgccaa actgcagctc acgtttcgcc ggataacgcc acggaataat
     2821 gtcatcatgc accacgatgg tcacttccac cgcacgcaga atttcgcttt cacccgggct
     2881 cgcgctggtt tccagcagat cgttaatcag cgcacgacgg gtggtttcat ccagacgcac
     2941 ggtcacggtc accagcagat caatatcgct atgcggtttc aggccgccat ccaccgcgct
     3001 gccatacaga tgcacggcca gcagggtcgg ttccagatga cgttcaatca cgcccaccac
     3061 ttcagacagc tgggtgctca cttccgcaat caccgcttca cgcataatgt tcagtttggt
     3121 tttcggacgg ctgccctgct gggtcacatc gttgctgctc cacagcatca ggcaacggcc
     3181 caccgcataa cgcgcctgct gtttgctcgc acgcggcatg ctctgcacgc caaaaaagca
     3241 atcatagcac gccataaaca ccgccaccgc accattaccg c
//
