LOCUS       sequence-271564-        3959 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Golden Gate Level 0 S module for N-terminal fluorescent protein 
            tagging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3959)
  AUTHORS   Luginbuehl LH, El-Sharnouby S, Wang N, Hibberd JM
  TITLE     Fluorescent reporters for functional analysis in rice leaves.
  JOURNAL   Plant Direct. 2020 Feb 11;4(2):e00188. doi: 10.1002/pld3.188. 
            eCollection 2020 Feb.
  PUBMED    32072132
REFERENCE   2  (bases 1 to 3959)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Direct."; date: "2020-02-11"; volume: "4(2):e00188. doi"; pages: " 
            10.1002/pld3.188. eCollection 2020 Feb"
COMMENT     Sequence Label: pL0M-S-tdTomato-EC18158
FEATURES             Location/Qualifiers
     source          1..3959
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     146..168
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     160..177
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     161..177
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     188..207
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        188..206
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1817..1834)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1927..2515)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2007..2026)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        complement(3700..3804)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tggccgctac agggcgctcc cattcgccat tcaggctgcg caactgttgg gaagggcgtt
       61 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      121 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgag
      181 cgcgacgtaa tacgactcac tatagggcga attgagtgaa ggccgtcaag gccgcatcac
      241 tctgtggtct caaatggcga gcaagggcga ggaggttatt aaggaattca tgaggttcaa
      301 agtgcggatg gagggctcca tgaatggcca tgaatttgag attgagggtg agggtgaagg
      361 taggccttac gagggaacgc agactgcaaa gttgaaagtg accaagggag ggcccctccc
      421 ttttgcgtgg gacatcctct cgccccagtt catgtatggt agcaaggcgt acgtgaagca
      481 tccggcagac attccggact ataaaaagct gtcttttcct gagggcttta agtgggagcg
      541 ggttatgaat tttgaggacg gtggccttgt tacagtgaca caagactcga gccttcaaga
      601 cgggactctg atatataagg ttaaaatgag gggtactaat tttcccccgg acggcccggt
      661 gatgcaaaag aaaaccatgg ggtgggaggc gtcaactgag cgcctctatc cgcgcgacgg
      721 ggtgctcaag ggcgaaattc accaggcatt gaaactcaag gatggtggtc attatcttgt
      781 cgaatttaaa acgatataca tggccaaaaa acccgtgcag ttgcccggtt attattatgt
      841 cgatactaag ctcgatatta catcgcacaa cgaggactac acgattgtcg aacagtacga
      901 acgcagcgaa ggtagacacc accttttttt ggggcatggt actggttcga cgggatcggg
      961 gtcgtcgggt acggcatcat cggaggacaa caacatggcg gtgattaaag agttcatgag
     1021 gttcaaagtt aggatggagg ggtctatgaa tggacatgaa ttcgaaattg agggcgaggg
     1081 ggaaggacgc ccttatgagg ggactcagac agcgaagctt aaggttacta agggaggacc
     1141 tctgccgttt gcttgggata ttctgagccc gcagttcatg tatggatcga aagcctatgt
     1201 taaacatccc gctgacatcc cagactacaa aaagctttca tttccggaag gtttcaaatg
     1261 ggaaagagtc atgaatttcg aagatggagg cctcgttact gttacgcaag acagctctct
     1321 tcaggacggg acattgatat ataaggttaa gatgcgcggg accaactttc cgcctgatgg
     1381 tccggtcatg caaaaaaaga ctatgggctg ggaggcgagt acagaaaggc tttacccaag
     1441 agatggcgtg cttaagggtg aaatacatca agctcttaaa cttaaagatg gagggcatta
     1501 tctcgttgag ttcaaaacga tctatatggc taagaaaccg gttcagctgc ctggctatta
     1561 ttatgttgat accaaattgg atattacaag tcataacgag gactatacta tagttgaaca
     1621 gtatgaaagg tccgagggta ggcatcacct gttcctttat ggaatggacg aactctataa
     1681 gggaggttga gaccacgaag tgctgggcct catgggcctt cacttcactg cccgctttcc
     1741 agtcgggaaa cctgtcgtgc cagctgcatt aacatggtca tagctgtttc cttgcgtatt
     1801 gggcgctctc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg gtaaagcctg
     1861 gggtgcctaa tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     1921 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     1981 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     2041 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     2101 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     2161 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     2221 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     2281 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     2341 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     2401 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     2461 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     2521 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     2581 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     2641 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttatt atttacccac
     2701 cactttggta atttcgcctt tcacgtaatg cacaaattct tccagctgat ccgcacggct
     2761 ggccagacga tcttcttcct ggcccagata cgcctgacgc gcttccagaa tcaccggctg
     2821 atactgcgcc ggcagacgtt ccatggccca atccgccgcc acatctttcg gcgcaatttt
     2881 gccggtcacc gcgctatacc aaatacggct cagggtcagc accacgttgc gttcatcacc
     2941 cgcccaatcc ggcgggctgt tccacagggt cagggtttcg ttcagcgctt caaacaggtc
     3001 ctgttccggc accggatcaa acagttcttc cgctgccgga ccaaccagcg ccacgctatg
     3061 ttcacgcgct ttggtcagca gaatggccag atcgatatca atggtcgccg gttcaaaaat
     3121 gccggccaga atatcgttac gctgccattc gccaaactgc agctcacgtt tcgccggata
     3181 acgccacgga ataatgtcat catgcaccac gatggtcact tccaccgcac gcagaatttc
     3241 gctttcaccc gggctcgcgc tggtttccag cagatcgtta atcagcgcac gacgggtggt
     3301 ttcatccaga cgcacggtca cggtcaccag cagatcaata tcgctatgcg gtttcaggcc
     3361 gccatccacc gcgctgccat acagatgcac ggccagcagg gtcggttcca gatgacgttc
     3421 aatcacgccc accacttcag acagctgggt gctcacttcc gcaatcaccg cttcacgcat
     3481 aatgttcagt ttggttttcg gacggctgcc ctgctgggtc acatcgttgc tgctccacag
     3541 catcaggcaa cggcccaccg cataacgcgc ctgctgtttg ctcgcacgcg gcatgctctg
     3601 cacgccaaaa aagcaatcat agcacgccat aaacaccgcc accgcaccat taccgccgct
     3661 acgttcggtc agggtacggc tccaattacg gctacgcata ctcttccttt ttcaatatta
     3721 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     3781 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccaccta aattgtaagc
     3841 gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa
     3901 taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgag
//
