LOCUS       sequence-271565-        5927 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Retroviral expression of H-RAS-V12.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5927)
  AUTHORS   Lock R, Roy S, Kenific CM, Su JS, Salas E, Ronen SM, Debnath J
  TITLE     Autophagy facilitates glycolysis during Ras-mediated oncogenic 
            transformation.
  JOURNAL   Mol Biol Cell. 2011 Jan 15;22(2):165-78. doi: 
            10.1091/mbc.E10-06-0500. Epub 2010 Nov 30.
  PUBMED    21119005
REFERENCE   2  (bases 1 to 5927)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E10-06-0500"; journalName: "Mol Biol Cell"; date: 
            "2011-01-15- 15"; volume: "22"; issue: "2"; pages: "165-78"
COMMENT     Sequence Label: pBABEneo-HRASV12
FEATURES             Location/Qualifiers
     source          1..5927
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             8..477
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    542..741
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             942..1358
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1288..1310
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1326..1342
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(2066..2086)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2071..2400
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2251..2386
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2757..3551
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2811..2830)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3421..3440
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     4074..4093
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     LTR             4355..4824
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     CDS             complement(join(4939..5730,5731..5799))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5562..5581
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5800..5904)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctgcagcctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca
       61 gggccaagaa cagatggaac agctgaatat gggccaaaca ggatatctgt ggtaagcagt
      121 tcctgccccg gctcagggcc aagaacagat ggtccccaga tgcggtccag ccctcagcag
      181 tttctagaga accatcagat gtttccaggg tgccccaagg acctgaaatg accctgtgcc
      241 ttatttgaac taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga
      301 gctcaataaa agagcccaca acccctcact cggggcgcca gtcctccgat tgactgagtc
      361 gcccgggtac ccgtgtatcc aataaaccct cttgcagttg catccgactt gtggtctcgc
      421 tgttccttgg gagggtctcc tctgagtgat tgactacccg tcagcggggg tctttcattt
      481 gggggctcgt ccgggatcgg gagacccctg cccagggacc accgacccac caccgggagg
      541 caagctggcc agcaacttat ctgtgtctgt ccgattgtct agtgtctatg actgatttta
      601 tgcgcctgcg tcggtactag ttagctaact agctctgtat ctggcggacc cgtggtggaa
      661 ctgacgagtt ctgaacaccc ggccgcaacc ctgggagacg tcccagggac tttgggggcc
      721 gtttttgtgg cccgacctga ggaagggagt cgatgtggaa tccgaccccg tcaggatatg
      781 tggttctggt aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt
      841 cggtttggaa ccgaagccgc gcgtcttgtc tgctgcagcg ctgcagcatc gttctgtgtt
      901 gtctctgtct gactgtgttt ctgtatttgt ctgaaaatta gggccagact gttaccactc
      961 ccttaagttt gaccttaggt cactggaaag atgtcgagcg gatcgctcac aaccagtcgg
     1021 tagatgtcaa gaagagacgt tgggttacct tctgctctgc agaatggcca acctttaacg
     1081 tcggatggcc gcgagacggc acctttaacc gagacctcat cacccaggtt aagatcaagg
     1141 tcttttcacc tggcccgcat ggacacccag accaggtccc ctacatcgtg acctgggaag
     1201 ccttggcttt tgacccccct ccctgggtca agccctttgt acaccctaag cctccgcctc
     1261 ctcttcctcc atccgccccg tctctccccc ttgaacctcc tcgttcgacc ccgcctcgat
     1321 cctcccttta tccagccctc actccttctc taggcgccgg ccggatccac cgacttctac
     1381 ctaaagagat ctgccaccat gactgaatac aagcttgtgg tggtgggcgc tgtaggcgtg
     1441 ggaaagagtg ccctgaccat ccagctgatc cagaaccact ttgtggacga gtatgatccc
     1501 actatagagg actcctaccg gaaacaggtg gtcattgatg gggagacatg tctactggac
     1561 atcttagaca cagcaggtca agaagagtat agtgccatgc gggaccagta catgcgcaca
     1621 ggggagggct tcctctgtgt atttgccatc aacaacacca agtccttcga ggacatccat
     1681 cagtacaggg agcagatcaa gcgggtgaaa gattcagatg atgtgccaat ggtgctggtg
     1741 ggcaacaagt gtgacctggc tgctcgcact gttgagtctc ggcaggccca ggaccttgct
     1801 cgcagctatg gcatccccta cattaaaaca tcagccaaga cccggcaggg cgtggaggat
     1861 gccttctata cactagtccg tgagattcgg cagcataaat tgcggaaact gaacccaccc
     1921 gatgagagtg gtcctggctg catgagctgc aaatgtgtgc tgtcctgaca ccaggctcag
     1981 gacatggagg tgccggatgc agggtggaca tgacccaggg ctcatctttg ggatcggaat
     2041 tcgccagcac agtggtcgac cctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca
     2101 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt
     2161 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     2221 gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     2281 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
     2341 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa
     2401 aagcttcacg ctgccgcaag cactcagggc gcaagggctg ctaaaggaag cggaacacgt
     2461 agaaagccag tccgcagaaa cggtgctgac cccggatgaa tgtcagctac tgggctatct
     2521 ggacaaggga aaacgcaagc gcaaagagaa agcaggtagc ttgcagtggg cttacatggc
     2581 gatagctaga ctgggcggtt ttatggacag caagcgaacc ggaattgcca gctggggcgc
     2641 cctctggtaa ggttgggaag ccctgcaaag taaactggat ggctttcttg ccgccaagga
     2701 tctgatggcg caggggatca agatctgatc aagagacagg atgaggatcg tttcgcatga
     2761 ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct
     2821 atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc
     2881 aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg
     2941 acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg
     3001 acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc
     3061 tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc
     3121 ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg
     3181 agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc
     3241 atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg
     3301 aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc
     3361 gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag
     3421 cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg
     3481 tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg
     3541 agttcttctg agcgggactc tggggttcgc atcgatgcat cgatgcggcg ggtgtggtgg
     3601 ttacgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     3661 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     3721 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     3781 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     3841 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     3901 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     3961 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     4021 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     4081 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     4141 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcatc gataaaataa
     4201 aagattttat ttagtctcca gaaaaagggg ggaatgaaag accccacctg taggtttggc
     4261 aagctagctt aagtaacgcc attttgcaag gcatggaaaa atacataact gagaatagag
     4321 aagttcagat caaggtcagg aacagatgga acagctgaat atgggccaaa caggatatct
     4381 gtggtaagca gttcctgccc cggctcaggg ccaagaacag atggaacagc tgaatatggg
     4441 ccaaacagga tatctgtggt aagcagttcc tgccccggct cagggccaag aacagatggt
     4501 ccccagatgc ggtccagccc tcagcagttt ctagagaacc atcagatgtt tccagggtgc
     4561 cccaaggacc tgaaatgacc ctgtgcctta tttgaactaa ccaatcagtt cgcttctcgc
     4621 ttctgttcgc gcgcttctgc tccccgagct caataaaaga gcccacaacc cctcactcgg
     4681 ggcgccagtc ctccgattga ctgagtcgcc cgggtacccg tgtatccaat aaaccctctt
     4741 gcagttgcat ccgacttgtg gtctcgctgt tccttgggag ggtctcctct gagtgattga
     4801 ctacccgtca gcgggggtct ttcacatgca gcatgtatca aaattaattt ggtttttttt
     4861 cttaagtatt tacattaaat ggccatagtg cggccgcaaa tcaatctaaa gtatatatga
     4921 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     4981 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     5041 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     5101 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     5161 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     5221 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     5281 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     5341 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     5401 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     5461 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     5521 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     5581 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     5641 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     5701 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     5761 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     5821 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     5881 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccac
//
