LOCUS       sequence-271568-         657 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR-Cas9 library validation (negative control).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 657)
  AUTHORS   Arnan C, Ullrich S, Pulido-Quetglas C, Nurtdinov R, Esteban A, 
            Blanco-Fernandez J, Aparicio-Prat E, Johnson R, Perez-Lluch S, Guigo
            R
  TITLE     Paired guide RNA CRISPR-Cas9 screening for protein-coding genes and 
            lncRNAs involved in transdifferentiation of human B-cells to 
            macrophages.
  JOURNAL   BMC Genomics. 2022 May 26;23(1):402. doi: 
            10.1186/s12864-022-08612-7.
  PUBMED    35619054
REFERENCE   2  (bases 1 to 657)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Genomics."; date: "2022-05-26"; volume: "23(1):402. doi"; pages: " 
            10.1186/s12864-022-08612-7"
COMMENT     Sequence Label: pDECKO_mCherry_non-targeting
FEATURES             Location/Qualifiers
     source          1..657
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     16..35
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        115..190
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        197..411
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     332..351
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     misc_RNA        440..515
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 tnatgtttta aaatggacta tcatatgctt accgtaactt gaaagtattt cgatttcttg
       61 gctttatata tcttgtggaa aggacgaaac accggaagtc gtactcgttg aagcgtttta
      121 gagctagaaa tagcaagtta aaataaggct agtccgttat caacttgaaa aagtggcacc
      181 gagtcggtgc ttttttgaac gctgacgtca tcaacccgct ccaaggaatc gcgggcccag
      241 tgtcactagg cgggaacacc cagcgcgcgt gcgccctggc aggaagatgg ctgtgaggga
      301 caggggagtg gcgccctgca atatttgcat gtcgctatgt gttctgggaa atcaccataa
      361 acgtgaaatg tctttggatt tgggagtctt ataagttctg tatgagacca ctctttcccg
      421 tgcggtaggg ctacgccctg ttttagagct agaaatagca agttaaaata aggctagtcc
      481 gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt taagcttggc gtaactagat
      541 cttgagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta
      601 cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta aagaatt
//
