LOCUS       sequence-27157-e         786 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 786)
  AUTHORS   Saks ME, Sampson JR, Nowak MW, Kearney PC, Du F, Abelson JN, Lester 
            HA, Dougherty DA
  TITLE     An engineered Tetrahymena tRNAGln for in vivo incorporation of 
            unnatural amino acids into proteins by nonsense suppression.
  JOURNAL   J Biol Chem. 1996 Sep 20. 271(38):23169-75.
  PUBMED    8798511
REFERENCE   2  (bases 1 to 786)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 1996 Sep 20. 271(38):23169-75."
COMMENT     Sequence Label: pUC19-THG73tRNA
FEATURES             Location/Qualifiers
     source          1..786
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(105..123)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(131..148)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(131..147)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(140..162)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(356..375)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     475..497
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(535..553)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        621..725
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gggtgactct agaggatccg gatgggtctc atggcggtcc tactgggatt cgaacccagg
       61 tcaagggatt tagagtcccc agtactaacc gctatactat agaacctata gtgagtcgta
      121 ttacgaattc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc
      181 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc
      241 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc ctgatgcggt
      301 attttctcct tacgcatctg tgcggtattt cacaccgcat atggtgcact ctcagtacaa
      361 tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc
      421 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga
      481 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg
      541 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg
      601 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
      661 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
      721 agagtatgag tattcaacat ttccgtgtcg cccttatccc tttttgcggc attttgcctc
      781 ctgttt
//
