LOCUS       sequence-271574-         614 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR-Cas9 library validation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 614)
  AUTHORS   Arnan C, Ullrich S, Pulido-Quetglas C, Nurtdinov R, Esteban A, 
            Blanco-Fernandez J, Aparicio-Prat E, Johnson R, Perez-Lluch S, Guigo
            R
  TITLE     Paired guide RNA CRISPR-Cas9 screening for protein-coding genes and 
            lncRNAs involved in transdifferentiation of human B-cells to 
            macrophages.
  JOURNAL   BMC Genomics. 2022 May 26;23(1):402. doi: 
            10.1186/s12864-022-08612-7.
  PUBMED    35619054
REFERENCE   2  (bases 1 to 614)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Genomics."; date: "2022-05-26"; volume: "23(1):402. doi"; pages: " 
            10.1186/s12864-022-08612-7"
COMMENT     Sequence Label: pDECKO_mCherry_LINC02432
FEATURES             Location/Qualifiers
     source          1..614
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     38..57
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        138..213
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        220..434
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     355..374
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     misc_RNA        463..538
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 tggggtagtt tgcagtttta aattatgttt taaaatggac tatcatatgc ttaccgtaac
       61 ttgaaagtat ttcgatttct tggctttata tatcttgtgg aaaggacgaa acaccggcct
      121 tttataaaga tactgaggtt ttagagctag aaatagcaag ttaaaataag gctagtccgt
      181 tatcaacttg aaaaagtggc accgagtcgg tgcttttttg aacgctgacg tcatcaaccc
      241 gctccaagga atcgcgggcc cagtgtcact aggcgggaac acccagcgcg cgtgcgccct
      301 ggcaggaaga tggctgtgag ggacagggga gtggcgccct gcaatatttg catgtcgcta
      361 tgtgttctgg gaaatcacca taaacgtgaa atgtctttgg atttgggagt cttataagtt
      421 ctgtatgaga ccactctttc cccctgtagt atacgtaata acgttttaga gctagaaata
      481 gcaagttaaa ataaggctag tccgttatca acttgaaaaa gtggcaccga gtcggtgctt
      541 ttttaagctt ggcgtaacta gatcttgaga caaatggcag tattcatcca caattttaaa
      601 agaaaagggg ggat
//
