LOCUS       sequence-271578-         700 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR-Cas9 Knock down.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 700)
  AUTHORS   Arnan C, Ullrich S, Pulido-Quetglas C, Nurtdinov R, Esteban A, 
            Blanco-Fernandez J, Aparicio-Prat E, Johnson R, Perez-Lluch S, Guigo
            R
  TITLE     Paired guide RNA CRISPR-Cas9 screening for protein-coding genes and 
            lncRNAs involved in transdifferentiation of human B-cells to 
            macrophages.
  JOURNAL   BMC Genomics. 2022 May 26;23(1):402. doi: 
            10.1186/s12864-022-08612-7.
  PUBMED    35619054
REFERENCE   2  (bases 1 to 700)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Genomics."; date: "2022-05-26"; volume: "23(1):402. doi"; pages: " 
            10.1186/s12864-022-08612-7"
COMMENT     Sequence Label: pDECKO_mCherry_TREML2
FEATURES             Location/Qualifiers
     source          1..700
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        135..210
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        217..431
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     352..371
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     misc_RNA        460..535
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 tggggagttt gnagttttaa attatgtttt aaatggacta tcatatgctt accgtaactt
       61 gaaagtattt cgatttcttg gctttatata tcttgtggaa aggacgaaac accgggtgaa
      121 caccatcaca ccaggtttta gagctagaaa tagcaagtta aaataaggct agtccgttat
      181 caacttgaaa aagtggcacc gagtcggtgc ttttttgaac gctgacgtca tcaacccgct
      241 ccaaggaatc gcgggcccag tgtcactagg cgggaacacc cagcgcgcgt gcgccctggc
      301 aggaagatgg ctgtgaggga caggggagtg gcgccctgca atatttgcat gtcgctatgt
      361 gttctgggaa atcaccataa acgtgaaatg tctttggatt tgggagtctt ataagttctg
      421 tatgagacca ctctttccct aggcaaggtg atgagtcctg ttttagagct agaaatagca
      481 agttaaaata aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt
      541 taagcttggc gtaactagat cttgagacaa atggcagtat tcatccacaa ttttaaaaga
      601 aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac
      661 atacaaacta aagaattaca aaaacaaatt acaaaaattc
//
