LOCUS       sequence-271579-         700 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR-Cas9 library validation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 700)
  AUTHORS   Arnan C, Ullrich S, Pulido-Quetglas C, Nurtdinov R, Esteban A, 
            Blanco-Fernandez J, Aparicio-Prat E, Johnson R, Perez-Lluch S, Guigo
            R
  TITLE     Paired guide RNA CRISPR-Cas9 screening for protein-coding genes and 
            lncRNAs involved in transdifferentiation of human B-cells to 
            macrophages.
  JOURNAL   BMC Genomics. 2022 May 26;23(1):402. doi: 
            10.1186/s12864-022-08612-7.
  PUBMED    35619054
REFERENCE   2  (bases 1 to 700)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Genomics."; date: "2022-05-26"; volume: "23(1):402. doi"; pages: " 
            10.1186/s12864-022-08612-7"
COMMENT     Sequence Label: pDECKO_mCherry_KLF4
FEATURES             Location/Qualifiers
     source          1..700
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     42..61
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        142..217
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        224..438
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     359..378
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     misc_RNA        467..542
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 anttctgggt agtttgcagt tttaaattat gttttaaaat ggactatcat atgcttaccg
       61 taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga cgaaacaccg
      121 gagtgtcttc tcccttcccg cgttttagag ctagaaatag caagttaaaa taaggctagt
      181 ccgttatcaa cttgaaaaag tggcaccgag tcggtgcttt tttgaacgct gacgtcatca
      241 acccgctcca aggaatcgcg ggcccagtgt cactaggcgg gaacacccag cgcgcgtgcg
      301 ccctggcagg aagatggctg tgagggacag gggagtggcg ccctgcaata tttgcatgtc
      361 gctatgtgtt ctgggaaatc accataaacg tgaaatgtct ttggatttgg gagtcttata
      421 agttctgtat gagaccactc tttcccctgg gggaagtcgc ttcatggttt tagagctaga
      481 aatagcaagt taaaataagg ctagtccgtt atcaacttga aaaagtggca ccgagtcggt
      541 gcttttttaa gcttggcgta actagatctt gagacaaatg gcagtattca tccacaattt
      601 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
      661 aacagacata caaactaaag aattacaaaa acaaattaca
//
