LOCUS       sequence-271582-         699 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR-Cas9 library validation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 699)
  AUTHORS   Arnan C, Ullrich S, Pulido-Quetglas C, Nurtdinov R, Esteban A, 
            Blanco-Fernandez J, Aparicio-Prat E, Johnson R, Perez-Lluch S, Guigo
            R
  TITLE     Paired guide RNA CRISPR-Cas9 screening for protein-coding genes and 
            lncRNAs involved in transdifferentiation of human B-cells to 
            macrophages.
  JOURNAL   BMC Genomics. 2022 May 26;23(1):402. doi: 
            10.1186/s12864-022-08612-7.
  PUBMED    35619054
REFERENCE   2  (bases 1 to 699)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Genomics."; date: "2022-05-26"; volume: "23(1):402. doi"; pages: " 
            10.1186/s12864-022-08612-7"
COMMENT     Sequence Label: pDECKO_mCherry_CEACAM1
FEATURES             Location/Qualifiers
     source          1..699
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     42..61
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        141..216
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        223..437
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     358..377
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     misc_RNA        466..541
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 atnctggggt agtttgcagt tttaaattat gttttaaaat ggactatcat atgcttaccg
       61 taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga cgaaacaccg
      121 gttattggcg tggcaggtat gttttagagc tagaaatagc aagttaaaat aaggctagtc
      181 cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt ttgaacgctg acgtcatcaa
      241 cccgctccaa ggaatcgcgg gcccagtgtc actaggcggg aacacccagc gcgcgtgcgc
      301 cctggcagga agatggctgt gagggacagg ggagtggcgc cctgcaatat ttgcatgtcg
      361 ctatgtgttc tgggaaatca ccataaacgt gaaatgtctt tggatttggg agtcttataa
      421 gttctgtatg agaccactct ttcccgacag ggtttatgct gaggggtttt agagctagaa
      481 atagcaagtt aaaataaggc tagtccgtta tcaacttgaa aaagtggcac cgagtcggtg
      541 cttttttaag cttggcgtaa ctagatcttg agacaaatgg cagtattcat ccacaatttt
      601 aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca
      661 acagacatac aaactaaaga attacaaaaa caaattaca
//
