LOCUS       sequence-271587-        4554 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses SFPQ 214-535 with TEV-cleavable hexahistidine tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4554)
  AUTHORS   Lee M, Sadowska A, Bekere I, Ho D, Gully BS, Lu Y, Iyer KS, 
            Trewhella J, Fox AH, Bond CS
  TITLE     The structure of human SFPQ reveals a coiled-coil mediated polymer 
            essential for functional aggregation in gene regulation.
  JOURNAL   Nucleic Acids Res. 2015 Apr 20;43(7):3826-40. doi: 
            10.1093/nar/gkv156. Epub 2015 Mar 12.
  PUBMED    25765647
REFERENCE   2  (bases 1 to 4554)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gkv156"; journalName: "Nucleic Acids Res"; date: 
            "2015-04-20- 20"; volume: "43"; issue: "7"; pages: "3826-40"
COMMENT     Sequence Label: pCDF11-SFPQ(214-535)
FEATURES             Location/Qualifiers
     source          1..4554
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             22..66
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     primer_bind     complement(104..122)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      118..165
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(336..1127)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        complement(1128..1219)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(1267..2005)
                     /direction=LEFT
                     /label=CloDF13 ori
                     /note="Plasmids containing the CloDF13 (CDF) origin of 
                     replication can be propagated in E. coli cells that contain
                     additional plasmids with compatible origins."
     CDS             complement(2215..3297)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     3259..3278
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(3298..3375)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     3421..3440
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3421..3439
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    3440..3464
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             3514..3531
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             3562..3582
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 taactgcagc tcgagtctgg taaagaaacc gctgctgcga aatttgaacg ccagcacatg
       61 gactcgtcta ctagcgcagc ttaattaacc taggctgctg ccaccgctga gcaataacta
      121 gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa acctcaggca
      181 tttgagaagc acacggtcac actgcttccg gtagtcaata aaccggtaaa ccagcaatag
      241 acataagcgg ctatttaacg accctgccct gaaccgacga ccgggtcatc gtggccggat
      301 cttgcggccc ctcggcttga acgaattgtt agacattatt tgccgactac cttggtgatc
      361 tcgcctttca cgtagtggac aaattcttcc aactgatctg cgcgcgaggc caagcgatct
      421 tcttcttgtc caagataagc ctgtctagct tcaagtatga cgggctgata ctgggccggc
      481 aggcgctcca ttgcccagtc ggcagcgaca tccttcggcg cgattttgcc ggttactgcg
      541 ctgtaccaaa tgcgggacaa cgtaagcact acatttcgct catcgccagc ccagtcgggc
      601 ggcgagttcc atagcgttaa ggtttcattt agcgcctcaa atagatcctg ttcaggaacc
      661 ggatcaaaga gttcctccgc cgctggacct accaaggcaa cgctatgttc tcttgctttt
      721 gtcagcaaga tagccagatc aatgtcgatc gtggctggct cgaagatacc tgcaagaatg
      781 tcattgcgct gccattctcc aaattgcagt tcgcgcttag ctggataacg ccacggaatg
      841 atgtcgtcgt gcacaacaat ggtgacttct acagcgcgga gaatctcgct ctctccaggg
      901 gaagccgaag tttccaaaag gtcgttgatc aaagctcgcc gcgttgtttc atcaagcctt
      961 acggtcaccg taaccagcaa atcaatatca ctgtgtggct tcaggccgcc atccactgcg
     1021 gagccgtaca aatgtacggc cagcaacgtc ggttcgagat ggcgctcgat gacgccaact
     1081 acctctgata gttgagtcga tacttcggcg atcaccgctt ccctcatact cttccttttt
     1141 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     1201 atttagaaaa ataaacaaat agctagctca ctcggtcgct acgctccggg cgtgagactg
     1261 cggcgggcgc tgcggacaca tacaaagtta cccacagatt ccgtggataa gcaggggact
     1321 aacatgtgag gcaaaacagc agggccgcgc cggtggcgtt tttccatagg ctccgccctc
     1381 ctgccagagt tcacataaac agacgctttt ccggtgcatc tgtgggagcc gtgaggctca
     1441 accatgaatc tgacagtacg ggcgaaaccc gacaggactt aaagatcccc accgtttccg
     1501 gcgggtcgct ccctcttgcg ctctcctgtt ccgaccctgc cgtttaccgg atacctgttc
     1561 cgcctttctc ccttacggga agtgtggcgc tttctcatag ctcacacact ggtatctcgg
     1621 ctcggtgtag gtcgttcgct ccaagctggg ctgtaagcaa gaactccccg ttcagcccga
     1681 ctgctgcgcc ttatccggta actgttcact tgagtccaac ccggaaaagc acggtaaaac
     1741 gccactggca gcagccattg gtaactggga gttcgcagag gatttgttta gctaaacacg
     1801 cggttgctct tgaagtgtgc gccaaagtcc ggctacactg gaaggacaga tttggttgct
     1861 gtgctctgcg aaagccagtt accacggtta agcagttccc caactgactt aaccttcgat
     1921 caaaccacct ccccaggtgg ttttttcgtt tacagggcaa aagattacgc gcagaaaaaa
     1981 aggatctcaa gaagatcctt tgatcttttc tactgaaccg ctctagattt cagtgcaatt
     2041 tatctcttca aatgtagcac ctgaagtcag ccccatacga tataagttgt aattctcatg
     2101 ttagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc
     2161 ggtcgagatc ccggtgccta atgagtgagc taacttacat taattgcgtt gcgctcactg
     2221 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     2281 gggagaggcg gtttgcgtat tgggcgccag ggtggttttt cttttcacca gtgagacggg
     2341 caacagctga ttgcccttca ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct
     2401 ggtttgcccc agcaggcgaa aatcctgttt gatggtggtt aacggcggga tataacatga
     2461 gctgtcttcg gtatcgtcgt atcccactac cgagatgtcc gcaccaacgc gcagcccgga
     2521 ctcggtaatg gcgcgcattg cgcccagcgc catctgatcg ttggcaacca gcatcgcagt
     2581 gggaacgatg ccctcattca gcatttgcat ggtttgttga aaaccggaca tggcactcca
     2641 gtcgccttcc cgttccgcta tcggctgaat ttgattgcga gtgagatatt tatgccagcc
     2701 agccagacgc agacgcgccg agacagaact taatgggccc gctaacagcg cgatttgctg
     2761 gtgacccaat gcgaccagat gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat
     2821 aatactgttg atgggtgtct ggtcagagac atcaagaaat aacgccggaa cattagtgca
     2881 ggcagcttcc acagcaatgg catcctggtc atccagcgga tagttaatga tcagcccact
     2941 gacgcgttgc gcgagaagat tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc
     3001 taccatcgac accaccacgc tggcacccag ttgatcggcg cgagatttaa tcgccgcgac
     3061 aatttgcgac ggcgcgtgca gggccagact ggaggtggca acgccaatca gcaacgactg
     3121 tttgcccgcc agttgttgtg ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc
     3181 ttccactttt tcccgcgttt tcgcagaaac gtggctggcc tggttcacca cgcgggaaac
     3241 ggtctgataa gagacaccgg catactctgc gacatcgtat aacgttactg gtttcacatt
     3301 caccaccctg aattgactct cttccgggcg ctatcatgcc ataccgcgaa aggttttgcg
     3361 ccattcgatg gtgtccggga tctcgacgct ctcccttatg cgactcctgc attaggaaat
     3421 taatacgact cactataggg gaattgtgag cggataacaa ttcccctgta gaaataattt
     3481 tgtttaactt taataaggag atataccatg aaacatcacc atcaccatca ccccatgagc
     3541 gattacgaca tccccactac tgagaatctt tattttcagg gcgccatggg cgggccgaag
     3601 ccaggtggcg gcccgggcct aagtacgcct ggcggccacc ccaagccgcc gcatcgaggc
     3661 ggcggggagc cccgcggggg ccgccagcac cacccgccct accaccagca gcatcaccag
     3721 gggcccccgc ccggcgggcc cggcggccgc agcgaggaga agatctcgga ctcggagggg
     3781 tttaaagcca atttgtctct cttgaggagg cctggagaga aaacttacac acagcgatgt
     3841 cggttgtttg ttgggaatct acctgctgat atcacggagg atgagttcaa aagactattt
     3901 gctaaatatg gagaaccagg agaagttttt atcaacaaag gcaaaggatt cggatttatt
     3961 aagcttgaat ctagagcttt ggctgaaatt gccaaagccg aactggatga tacacccatg
     4021 agaggtagac agcttcgagt tcgctttgcc acacatgctg ctgccctttc tgttcgtaat
     4081 ctttcacctt atgtttccaa tgaactgttg gaagaagcct ttagccaatt tggtcctatt
     4141 gaaagggctg ttgtaatagt ggatgatcgt ggaagatcta cagggaaagg cattgttgaa
     4201 tttgcttcta agccagcagc aagaaaggca tttgaacgat gcagtgaagg tgttttctta
     4261 ctgacgacaa ctcctcgtcc agtcattgtg gaaccacttg aacaactaga tgatgaagat
     4321 ggtcttcctg aaaaacttgc ccagaagaat ccaatgtatc aaaaggagag agaaacccct
     4381 cctcgttttg cccagcatgg cacgtttgag tacgaatatt ctcagcgatg gaagtctttg
     4441 gatgaaatgg aaaaacagca aagggaacaa gttgaaaaaa acatgaaaga tgcaaaagac
     4501 aaattggaaa gtgaaatgga agatgcctat catgaacatc aggcaaatct tttg
//
