LOCUS       sequence-271606-        3497 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid for pop-in/pop-out replacement of Gga2 with Gga2-HaloTagx2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3497)
  AUTHORS   Casler JC, Glick BS
  TITLE     A microscopy-based kinetic analysis of yeast vacuolar protein 
            sorting.
  JOURNAL   Elife. 2020 Jun 25;9. pii: 56844. doi: 10.7554/eLife.56844.
  PUBMED    32584255
REFERENCE   2  (bases 1 to 3497)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2020-06-25"; volume: "9. pii: 56844. doi"; pages: " 
            10.7554/eLife.56844"
COMMENT     Sequence Label: YIplac211-GGA2-HaloTagx2
FEATURES             Location/Qualifiers
     source          1..3497
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             781..1671
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="MAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSS
                     YVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVI
                     HDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLII
                     DQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALV
                     EEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDL
                     IGSEIARWLSTLEISG"
     CDS             1699..2589
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="MAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSS
                     YVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVI
                     HDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLII
                     DQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALV
                     EEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDL
                     IGSEIARWLSTLEISG"
ORIGIN
        1 ccgttacaac aatcttctgg tgctttaacg aatgaaatca acttaatcga tttcaatgat
       61 ttggacgaag cgccctctca aggcaacaac aataccaacg gcacgggtac accagcagca
      121 gcggaaacat ctgtcaatga cttattgggc gatttgaccg atttatctat ttcgaaccct
      181 tcaaccgcta accaagcatc ctttggtctg ggaggcgata tcgtgttggg gtcctctcaa
      241 cccgcaccac cagttactac tactaacaac tcgaacaaca ctttagatct tttgggactt
      301 tcaactcctc aatcgccaac taattcccaa gcagtaaaca gtagtgggtt cgatcttttg
      361 atgggattta atccaacaac aggtactact actgcgcccg caagaaccct agtcaatcag
      421 tctcctaact tgaagattga gttcgaaata tctagggagt caaactcagt tataaggata
      481 aaatcgttct ttacaaactt aagttcgtca ccgatctcca acttagtgtt cttattagca
      541 gtccctaagt caatgtcttt gaaattgcaa ccccaatcga gtaactttat gattggcaac
      601 gctaaagatg gtatctctca agaaggtaca attgaaaatg cgccagctaa cccttcaaag
      661 gctttgaaag tcaagtggaa ggtcaactat tctgtcaact ccacccaagc tgaagaaact
      721 gctgttttta cgttacctaa tgtaacggat ccggctagcg gaggaagttc ggggagttcg
      781 atggcagaaa tcggtactgg ctttccattc gacccccatt atgtggaagt cctgggcgag
      841 cgcatgcact acgtcgatgt tggtccgcgc gatggcaccc ctgtgctgtt cctgcacggt
      901 aacccgacct cctcctacgt gtggcgcaac atcatcccgc atgttgcacc gacccatcgc
      961 tgcattgctc cagacctgat cggtatgggc aaatccgaca aaccagacct gggttatttc
     1021 ttcgacgacc acgtccgctt catggatgcc ttcatcgaag ccctgggtct ggaagaggtc
     1081 gtcctggtca ttcacgactg gggctccgct ctgggtttcc actgggccaa gcgcaatcca
     1141 gagcgcgtca aaggtattgc atttatggag ttcatccgcc ctatcccgac ctgggacgaa
     1201 tggccagaat ttgcccgcga gaccttccag gccttccgca ccaccgacgt cggccgcaag
     1261 ctgatcatcg atcagaacgt ttttatcgag ggtacgctgc cgatgggtgt cgtccgcccg
     1321 ctgactgaag tcgagatgga ccattaccgc gagccgttcc tgaatcctgt tgaccgcgag
     1381 ccactgtggc gcttcccaaa cgagctgcca atcgccggtg agccagcgaa catcgtcgcg
     1441 ctggtcgaag aatacatgga ctggctgcac cagtcccctg tcccgaagct gctgttctgg
     1501 ggcaccccag gcgttctgat cccaccggcc gaagccgctc gcctggccaa aagcctgcct
     1561 aactgcaagg ctgtggacat cggcccgggt ctgaatctgc tgcaagaaga caacccggac
     1621 ctgatcggca gcgagatcgc gcgctggctg tcgacgctcg agatttccgg ctctagcgga
     1681 ggaagttcgg ggagttcgat ggcagaaatc ggtactggct ttccattcga cccccattat
     1741 gtggaagtcc tgggcgagcg catgcactac gtcgatgttg gtccgcgcga tggcacccct
     1801 gtgctgttcc tgcacggtaa cccgacctcc tcctacgtgt ggcgcaacat catcccgcat
     1861 gttgcaccga cccatcgctg cattgctcca gacctgatcg gtatgggcaa atccgacaaa
     1921 ccagacctgg gttatttctt cgacgaccac gtccgcttca tggatgcctt catcgaagcc
     1981 ctgggtctgg aagaggtcgt cctggtcatt cacgactggg gctccgctct gggtttccac
     2041 tgggccaagc gcaatccaga gcgcgtcaaa ggtattgcat ttatggagtt catccgccct
     2101 atcccgacct gggacgaatg gccagaattt gcccgcgaga ccttccaggc cttccgcacc
     2161 accgacgtcg gccgcaagct gatcatcgat cagaacgttt ttatcgaggg tacgctgccg
     2221 atgggtgtcg tccgcccgct gactgaagtc gagatggacc attaccgcga gccgttcctg
     2281 aatcctgttg accgcgagcc actgtggcgc ttcccaaacg agctgccaat cgccggtgag
     2341 ccagcgaaca tcgtcgcgct ggtcgaagaa tacatggact ggctgcacca gtcccctgtc
     2401 ccgaagctgc tgttctgggg caccccaggc gttctgatcc caccggccga agccgctcgc
     2461 ctggccaaaa gcctgcctaa ctgcaaggct gtggacatcg gcccgggtct gaatctgctg
     2521 caagaagaca acccggacct gatcggcagc gagatcgcgc gctggctgtc gacgctcgag
     2581 atttccggct ctagataagc ggccgctcct ctggcgtttc ttatcaatcc tttctcttct
     2641 ctatgtatat ttttagaagg ataatacctc tatgtagaca atcttataaa gatcttcgca
     2701 acaatacata tttcacttac taatatatgt tgcgttttca tgcatcaaaa gtagttagca
     2761 atgctagatc gtgtaaagga aaaaaaagag aaatagtata attcgccatc ctcataacca
     2821 ctgaaaaatc gaagttaacc aaaaggaaag ttgaaatgga ggaagtgttc cgtttttact
     2881 cgaactctag aaatatcttc attcataaaa gcctttcatt aaaaccatcc accattgatg
     2941 accccaaaag tggatatggc cttttcgtag aaccttcgaa gttcaagaat gatgaattga
     3001 aaagtgaaac tatccaattg ctccgaatcc ctaagcgctg cacgtttaat ataaacacac
     3061 ttctggctct cttgggagat gaggacgaat tttcttcaaa ggaagaattt caaagaacta
     3121 atgacaaaat taagattgct cttcgtgaaa taatggctca tccgaatttc agtgtgttcc
     3181 tgacagaaac aaatttactc ataatttatt tcatgatatt tcaaactatt cgtagtcgtt
     3241 atgagattcc cgaaaatatc caatattatt tagaaaatgt tttaatgagc attgaagtag
     3301 aaacagccat ggattctatt gaaaacttag ctactgatta tggacattat ccacagattt
     3361 ttggccttcg agagacgcta aatttattta aagaactgtt tcacgacgtc ttgaatttaa
     3421 gtgatatcaa acatctttat tctgcaataa tttcacgttg tttagagata ccagaaaggg
     3481 ccgataccaa aagtgaa
//
