LOCUS       sequence-271607-        6425 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid for pop-in/pop-out replacement of Sec7 with 
            Sec7-mScarlet2Ix6.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6425)
  AUTHORS   Casler JC, Glick BS
  TITLE     A microscopy-based kinetic analysis of yeast vacuolar protein 
            sorting.
  JOURNAL   Elife. 2020 Jun 25;9. pii: 56844. doi: 10.7554/eLife.56844.
  PUBMED    32584255
REFERENCE   2  (bases 1 to 6425)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2020-06-25"; volume: "9. pii: 56844. doi"; pages: " 
            10.7554/eLife.56844"
COMMENT     Sequence Label: YIplac211-Sec7-mScarlet2Ix6
FEATURES             Location/Qualifiers
     source          1..6425
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 aattctattg ttgtcaaatg tgttttacag cttttgatga tagagctgct caacgaatta
       61 ttcgagaacg aagattttgc ccactgtatc ccttacaagg aagccattag aattacaaga
      121 ttgttggaga aatcgtacga attttctcgt gactttaatg aagattatgg gttaagaaca
      181 agactagtag aggctcgtgt agttgataaa ataccaaacc tgttgaaaca agaaacgagt
      241 gctgctgcag ttctccttga cattatgttt cagttgtatc tgaacgatga tgagaagaag
      301 gctgatttaa taactcgtct gatcaccatt tgcatccagg tcgtagaggg ttatgtttct
      361 ttggatgata gaaccatgga acgtagtatc aatgcctggc gttcagtgat agttgaaata
      421 ttacaaggtt actacgaatt tgatgatgaa gactttagac tatactgtcc tgctatgtat
      481 gctctggtga ttcaaatatt agataagagc gttccaacgg agttgagaca tgctataaaa
      541 caatttctaa gcagagttgg tgaattatac ctttctacgg atccaactag tatggatagt
      601 acagaagcag ttataaagga attcatgaga ttcaaagtcc acatggaagg tagtatgaac
      661 ggtcacgaat tcgaaataga aggtgaaggt gaaggtagac catatgaagg tactcaaaca
      721 gctaaattga aggttactaa aggtggtcca ttgccatttt cttgggatat cttgtctcca
      781 caattcatgt acggttcaag agcttttatt aaacatccag cagatattcc agattactac
      841 aagcaatcat ttccagaagg ttttaaatgg gaaagagtta tgaacttcga agatggtggt
      901 gcagttactg ttacacaaga tacttctttg gaagatggta cattgatcta taaggttaaa
      961 ttgagaggta caaattttcc accagatggt ccagttatgc aaaagaaaac tatgggttgg
     1021 gaagcatcta cagaaagatt gtacccagaa gatggtgttt tgaagggtga catcaagatg
     1081 gcattgagat tgaaggatgg tggtagatat ttggctgatt tcaagactac atacaaggct
     1141 aagaaaccag ttcaaatgcc aggtgcttac aacgttgata gaaaattgga tatcacttct
     1201 cataacgaag attacacagt tgttgaacaa tacgaaagaa gtgaaggtag acatagtaca
     1261 ggtggtagtg gtgctagtat ggatagtaca gaagcagtta taaaggaatt catgagattc
     1321 aaagtccaca tggaaggtag tatgaacggt cacgaattcg aaatagaagg tgaaggtgaa
     1381 ggtagaccat atgaaggtac tcaaacagct aaattgaagg ttactaaagg tggtccattg
     1441 ccattttctt gggatatctt gtctccacaa ttcatgtacg gttcaagagc ttttattaaa
     1501 catccagcag atattccaga ttactacaag caatcatttc cagaaggttt taaatgggaa
     1561 agagttatga acttcgaaga tggtggtgca gttactgtta cacaagatac ttctttggaa
     1621 gatggtacat tgatctataa ggttaaattg agaggtacaa attttccacc agatggtcca
     1681 gttatgcaaa agaaaactat gggttgggaa gcatctacag aaagattgta cccagaagat
     1741 ggtgttttga agggtgacat caagatggca ttgagattga aggatggtgg tagatatttg
     1801 gctgatttca agactacata caaggctaag aaaccagttc aaatgccagg tgcttacaac
     1861 gttgatagaa aattggatat cacttctcat aacgaagatt acacagttgt tgaacaatac
     1921 gaaagaagtg aaggtagaca tagtacaggt ggtagtggtg ctagtatgga tagtacagaa
     1981 gcagttataa aggaattcat gagattcaaa gtccacatgg aaggtagtat gaacggtcac
     2041 gaattcgaaa tagaaggtga aggtgaaggt agaccatatg aaggtactca aacagctaaa
     2101 ttgaaggtta ctaaaggtgg tccattgcca ttttcttggg atatcttgtc tccacaattc
     2161 atgtacggtt caagagcttt tattaaacat ccagcagata ttccagatta ctacaagcaa
     2221 tcatttccag aaggttttaa atgggaaaga gttatgaact tcgaagatgg tggtgcagtt
     2281 actgttacac aagatacttc tttggaagat ggtacattga tctataaggt taaattgaga
     2341 ggtacaaatt ttccaccaga tggtccagtt atgcaaaaga aaactatggg ttgggaagca
     2401 tctacagaaa gattgtaccc agaagatggt gttttgaagg gtgacatcaa gatggcattg
     2461 agattgaagg atggtggtag atatttggct gatttcaaga ctacatacaa ggctaagaaa
     2521 ccagttcaaa tgccaggtgc ttacaacgtt gatagaaaat tggatatcac ttctcataac
     2581 gaagattaca cagttgttga acaatacgaa agaagtgaag gtagacatag tacaggtggt
     2641 agtggtgcta gtatggatag tacagaagca gttataaagg aattcatgag attcaaagtc
     2701 cacatggaag gtagtatgaa cggtcacgaa ttcgaaatag aaggtgaagg tgaaggtaga
     2761 ccatatgaag gtactcaaac agctaaattg aaggttacta aaggtggtcc attgccattt
     2821 tcttgggata tcttgtctcc acaattcatg tacggttcaa gagcttttat taaacatcca
     2881 gcagatattc cagattacta caagcaatca tttccagaag gttttaaatg ggaaagagtt
     2941 atgaacttcg aagatggtgg tgcagttact gttacacaag atacttcttt ggaagatggt
     3001 acattgatct ataaggttaa attgagaggt acaaattttc caccagatgg tccagttatg
     3061 caaaagaaaa ctatgggttg ggaagcatct acagaaagat tgtacccaga agatggtgtt
     3121 ttgaagggtg acatcaagat ggcattgaga ttgaaggatg gtggtagata tttggctgat
     3181 ttcaagacta catacaaggc taagaaacca gttcaaatgc caggtgctta caacgttgat
     3241 agaaaattgg atatcacttc tcataacgaa gattacacag ttgttgaaca atacgaaaga
     3301 agtgaaggta gacatagtac aggtggtagt ggtgctagta tggatagtac agaagcagtt
     3361 ataaaggaat tcatgagatt caaagtccac atggaaggta gtatgaacgg tcacgaattc
     3421 gaaatagaag gtgaaggtga aggtagacca tatgaaggta ctcaaacagc taaattgaag
     3481 gttactaaag gtggtccatt gccattttct tgggatatct tgtctccaca attcatgtac
     3541 ggttcaagag cttttattaa acatccagca gatattccag attactacaa gcaatcattt
     3601 ccagaaggtt ttaaatggga aagagttatg aacttcgaag atggtggtgc agttactgtt
     3661 acacaagata cttctttgga agatggtaca ttgatctata aggttaaatt gagaggtaca
     3721 aattttccac cagatggtcc agttatgcaa aagaaaacta tgggttggga agcatctaca
     3781 gaaagattgt acccagaaga tggtgttttg aagggtgaca tcaagatggc attgagattg
     3841 aaggatggtg gtagatattt ggctgatttc aagactacat acaaggctaa gaaaccagtt
     3901 caaatgccag gtgcttacaa cgttgataga aaattggata tcacttctca taacgaagat
     3961 tacacagttg ttgaacaata cgaaagaagt gaaggtagac atagtacagg tggtagtggt
     4021 gctagtatgg atagtacaga agcagttata aaggaattca tgagattcaa agtccacatg
     4081 gaaggtagta tgaacggtca cgaattcgaa atagaaggtg aaggtgaagg tagaccatat
     4141 gaaggtactc aaacagctaa attgaaggtt actaaaggtg gtccattgcc attttcttgg
     4201 gatatcttgt ctccacaatt catgtacggt tcaagagctt ttattaaaca tccagcagat
     4261 attccagatt actacaagca atcatttcca gaaggtttta aatgggaaag agttatgaac
     4321 ttcgaagatg gtggtgcagt tactgttaca caagatactt ctttggaaga tggtacattg
     4381 atctataagg ttaaattgag aggtacaaat tttccaccag atggtccagt tatgcaaaag
     4441 aaaactatgg gttgggaagc atctacagaa agattgtacc cagaagatgg tgttttgaag
     4501 ggtgacatca agatggcatt gagattgaag gatggtggta gatatttggc tgatttcaag
     4561 actacataca aggctaagaa accagttcaa atgccaggtg cttacaacgt tgatagaaaa
     4621 ttggatatca cttctcataa cgaagattac acagttgttg aacaatacga aagaagtgaa
     4681 ggtagacata gtacaggtgg tagtggtgct agctaagcgg ccgggctttg ttgaatggtc
     4741 cagcagatta aaatatgctt agttgtagaa cacagaaaaa atgagaaggt tgatatactt
     4801 ttactcatat taatcctgat ttagtggcgg tgatcctttt tagattcgtt taattcataa
     4861 acaaggtaca tcatatagca ataacaaata tgtaaactat gtagaaagac ttatcaagtt
     4921 tagccatttt ggtcctcatt catgggcatt ttatactaac ttcatttttg ccgaaataag
     4981 aaaggatgta atcttaacct aaaatatgta agcaattaag ggcgcacgta gtcacttgcg
     5041 aacaaatagg aaaagaaaga aacaagcacg gatactgaat gactgagtgt attactacac
     5101 gatttatctc aacgaattta agtgtggagg acgatcggct gcacgtagga gtcaacgttt
     5161 ttttactgtg ttcatcacaa aaccattaag ctgagtcatc catattgcaa acaataaggt
     5221 agcaccgctt ccatcaaaac actagaacat accgatgttt tcacggattt attggaaaca
     5281 aatagcagat gagggcgttt ttacttgtaa tgaaggcgaa tgagaaggca aggcttacag
     5341 gcttgttgtt tatgccaagc atcaatactc gctatggagg agcgccatca gcgataacaa
     5401 caaagtttgc aagtgcttgc ttggatataa aaggcttgac gcttaagata aatagggcca
     5461 tacttgcttg cttttactat ttttgttcct cataagcctg attgactacg ctattataaa
     5521 cccctacatt atcctataat caacttcact tgtgtaacgt tgaatgagtt caactgagta
     5581 atactcggtc ggaaatatac gctccatctg gcaggaaaca taggctgcat ttatatcttg
     5641 cacaattatg cccttcgtag gtgtatgttt tcatgttttc cattatgtca atgtagatgt
     5701 atctttggac aggcaacttt tggatcaata aagcacaatt tttgagcaat ttgagatatc
     5761 cagtatttgt acgggagtcc caagattgtt tagcctaaaa ggttgaggac ttggattatt
     5821 cttccatcaa gcgcctccac ccctcgcatc ctatttcttt tgaagcatag gggacgagac
     5881 tgtctgtaat ttatggcgaa gtatgacaag aaagattcaa actttgtttt acgtatgccc
     5941 caataaattc tatggtagca ctactagaga cgtattttgg ttttgtaata attttcaaac
     6001 tgctcgctat gatttgttac aagaaacaaa caaaagacac gaccataggg ttcatgttgg
     6061 tggttgcgaa gccacatctt taaaacatta ctgcaaattc agcaggagtg agtagcaaaa
     6121 tcgacaaaaa ctagagtacg caacattccc ggtcgtaaat ttcttatcgt ggcgccagaa
     6181 cattcccgtc caatgatgag aatgtctcta aaacaaatgc agtcacagag attttctgcg
     6241 acttctcgag tgagaatgtt gcttattggt gctagtggct tgagatcttc gtcaagcaaa
     6301 ttggaatgcc ccagattctc aaacaagcac ggcagaaact gaaatacggc caaaattatg
     6361 aacccttttg tgggatcttt gtagaaatag gacctcagga tagtatgatg aagaatgttg
     6421 gcatg
//
